FastQCFastQC Report
Fri 27 May 2016
SRR2139154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8273599
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139100.046.8614271
GTATCAA251250.037.0353361
TCAACGC292350.031.4077514
ATCAACG294550.031.1134763
CAACGCA299300.030.6490635
TATCAAC304750.030.1874892
AACGCAG307700.029.8723936
CGCAGAG349300.026.314898
ACGCAGA349750.026.2684637
GCAGAGT402550.022.768399
GTACATG239900.021.454981
GAGTACT264800.021.33925212-13
TACATGG232700.021.2688242
ACATGGG233850.020.4311893
GTGGTAT73500.019.9737971
AGAGTAC358350.019.8112210-11
CAGAGTA392800.019.7859510-11
TGGTATC71100.019.4734292
GTACTTT294900.019.14564514-15
CATGGGG163250.018.443314
Produced by FastQC (version 0.11.5)