##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139154_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8273599
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71123521940089	33.0	33.0	33.0	33.0	33.0
2	32.72661776332162	33.0	33.0	33.0	33.0	33.0
3	32.700037674052126	33.0	33.0	33.0	33.0	33.0
4	36.64788346643341	37.0	37.0	37.0	37.0	37.0
5	36.67410712073428	37.0	37.0	37.0	37.0	37.0
6	36.64220165855271	37.0	37.0	37.0	37.0	37.0
7	36.54812760444397	37.0	37.0	37.0	37.0	37.0
8	36.583160000865405	37.0	37.0	37.0	37.0	37.0
9	36.635049994567055	37.0	37.0	37.0	37.0	37.0
10-11	36.61812072352068	37.0	37.0	37.0	37.0	37.0
12-13	36.604072907086746	37.0	37.0	37.0	37.0	37.0
14-15	38.7762329307959	40.0	37.0	40.0	37.0	40.0
16-17	38.72101409555866	40.0	37.0	40.0	37.0	40.0
18-19	38.744543275544295	40.0	37.0	40.0	37.0	40.0
20-21	38.754597455040724	40.0	37.0	40.0	37.0	40.0
22-23	38.732358581269594	40.0	37.0	40.0	37.0	40.0
24-25	38.752891554706274	40.0	37.0	40.0	37.0	40.0
26-27	38.68499808231093	40.0	37.0	40.0	37.0	40.0
28-29	38.58340586470298	40.0	37.0	40.0	37.0	40.0
30-31	38.45189589105038	40.0	37.0	40.0	37.0	40.0
32-33	38.360769654082205	40.0	37.0	40.0	37.0	40.0
34-35	38.33013595317209	40.0	37.0	40.0	37.0	40.0
36-37	38.3042845694644	40.0	37.0	40.0	37.0	40.0
38-39	38.26309390644009	40.0	37.0	40.0	37.0	40.0
40-41	38.22519101862774	40.0	37.0	40.0	37.0	40.0
42-43	38.175453993794754	40.0	37.0	40.0	37.0	40.0
44-45	38.07950629268126	40.0	37.0	40.0	35.0	40.0
46-47	37.994080319605374	40.0	37.0	40.0	33.0	40.0
48-49	37.931873113158844	40.0	37.0	40.0	33.0	40.0
50-51	37.888504537760575	40.0	37.0	40.0	33.0	40.0
52-53	37.82368822957638	40.0	37.0	40.0	33.0	40.0
54-55	37.72936143831333	40.0	37.0	40.0	33.0	40.0
56-57	37.57376253294786	40.0	37.0	40.0	33.0	40.0
58-59	37.37508923317499	40.0	37.0	40.0	33.0	40.0
60-61	37.21608442865474	37.0	37.0	40.0	33.0	40.0
62-63	37.07998067853901	37.0	37.0	40.0	33.0	40.0
64-65	36.908932265713744	37.0	37.0	40.0	33.0	40.0
66-67	36.70345209952753	37.0	37.0	40.0	33.0	40.0
68-69	36.50620117568025	37.0	37.0	40.0	33.0	40.0
70-71	36.28619368401496	37.0	37.0	40.0	33.0	40.0
72-73	35.997305133193606	37.0	37.0	37.0	33.0	40.0
74-75	35.663114275053005	37.0	35.0	37.0	33.0	40.0
76-77	33.53877012434631	35.0	33.0	37.0	27.0	37.0
78-79	35.17210157706623	37.0	33.0	37.0	33.0	37.0
80-81	35.37024725182109	37.0	33.0	37.0	33.0	37.0
82-83	35.23111039557474	37.0	33.0	37.0	33.0	37.0
84-85	35.05441768730152	37.0	33.0	37.0	33.0	37.0
86-87	34.93276456404619	37.0	33.0	37.0	33.0	37.0
88-89	34.826269275113894	37.0	33.0	37.0	33.0	37.0
90-91	34.73445547264707	37.0	33.0	37.0	33.0	37.0
92-93	34.71318776494611	37.0	33.0	37.0	33.0	37.0
94-95	34.673610943508606	37.0	33.0	37.0	33.0	37.0
96-97	34.674697473833916	37.0	33.0	37.0	33.0	37.0
98-99	34.70354519603248	37.0	33.0	37.0	33.0	37.0
100	34.68113010569242	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	3.0
20	29.0
21	155.0
22	749.0
23	2232.0
24	5517.0
25	11614.0
26	20700.0
27	33547.0
28	51348.0
29	73432.0
30	102244.0
31	137640.0
32	184210.0
33	252291.0
34	356237.0
35	532985.0
36	965281.0
37	2455899.0
38	3006235.0
39	81249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.545027744274286	20.16106896164535	14.87753999196722	23.416363302113144
2	14.168659763709822	20.528778677511824	43.13522504712605	22.167336511652305
3	18.474233522799448	25.860595854355523	30.2595883605188	25.405582262326227
4	12.158481454080624	17.92148737206142	39.293069436891976	30.62696173696598
5	13.209390496203646	37.55243637019392	35.65697346463129	13.581199668971145
6	29.58112908300245	37.96732232248626	18.932087474870368	13.519461119640921
7	26.37580090599025	32.75660326298144	23.132375644504886	17.735220186523424
8	23.68892908636254	37.595138463926034	21.03384512592404	17.68208732378739
9	24.95772396027412	17.743535793794212	21.78808762667855	35.51065261925312
10-11	24.00613082650005	27.179145375549385	28.730272037598148	20.08445176035242
12-13	24.87089389148366	24.581429559146336	29.25405896744123	21.29361758192877
14-15	21.75759303780616	25.96018371207016	27.343886257963433	24.93833699216024
16-17	21.028786867722257	29.123855289578334	28.017142237616305	21.830215605083108
18-19	20.948434895140554	28.044028964903905	30.218638829365553	20.788897310589984
20-21	21.71394154775601	27.15681641781402	30.348133026510794	20.78110900791917
22-23	21.424252191567042	27.079919405670914	30.21745201936396	21.278376383398083
24-25	21.184219394769922	27.243816329176784	30.293686772594874	21.27827750345842
26-27	21.168084783719713	27.41068813365089	30.23714267815995	21.184084404469445
28-29	21.03401168265475	27.59616852289672	30.173308703924445	21.19651109052409
30-31	21.299193907803204	27.396810267986897	30.13682736838026	21.167168455829636
32-33	21.111036256777744	27.534336460903692	30.08411895516475	21.27050832715381
34-35	21.28468093118171	27.594250870022048	30.00742783220266	21.113640366593582
36-37	21.27556989922152	27.529311655637066	29.971885188894426	21.22323325624699
38-39	21.151001583675217	27.74180991191857	29.94643981682778	21.160748687578433
40-41	21.432621962478596	27.63364934683958	29.705852365978902	21.227876324702926
42-43	21.319055147112227	27.71168425854959	29.505620530885775	21.463640063452406
44-45	21.445940976528448	27.90974677089732	29.21548782441005	21.428824428164184
46-47	21.49186905073813	28.00279469815426	28.884144035112747	21.62119221599486
48-49	21.502253127046675	28.051377012419636	28.81744458578189	21.6289252747518
50-51	21.535599981871943	28.168634195943575	28.64725518618692	21.64851063599756
52-53	21.62775073991914	28.189212392304274	28.459108166038227	21.72392870173836
54-55	21.653288944652388	28.08252326971445	28.504597116718735	21.75959066891443
56-57	21.667097965078852	28.164325969815913	28.4364646447907	21.73211142031454
58-59	21.668130398659603	28.14953310067384	28.503417111595063	21.678919389071496
60-61	21.719912837029103	28.12641436178913	28.49737045330742	21.65630234787435
62-63	21.67144854833253	28.20218183423758	28.425911539266696	21.70045807816319
64-65	21.63202474930382	28.20555859369369	28.3626832195341	21.799733437468387
66-67	21.742213178419664	28.15259086329946	28.232082728099527	21.873113230181353
68-69	21.686668109365552	28.191761740589143	28.269697839060143	21.85187231098516
70-71	21.73356939416325	27.98906843279077	28.309552503668016	21.967809669377964
72-73	21.78449460591398	28.01612149395116	28.28771965421743	21.91166424591743
74-75	21.657518568629484	28.137738284824003	28.218382619503856	21.986360527042656
76-77	21.784567019513336	28.09453694767816	28.109896912287063	22.010999120521443
78-79	21.76805435011964	28.067421185708174	28.107603523589276	22.05692094058291
80-81	21.776725725046507	28.117588740214767	28.107543491751823	21.998142042986903
82-83	21.848179258108033	28.060948335487556	28.179280367894293	21.911592038510115
84-85	21.986465994288192	28.015287987706973	28.087411727348126	21.910834290656705
86-87	21.778708186745174	28.097889718305	28.157446782293295	21.965955312656526
88-89	21.91213926685049	28.055786023583945	28.062404898396693	21.969669811168867
90-91	21.949327836703667	28.037290990642443	28.069727126842952	21.94365404581094
92-93	21.95909653505679	28.045000856461016	28.068215746740634	21.92768686174156
94-95	21.831488479816315	28.09739597714656	28.11013718728844	21.960978355748686
96-97	21.87751290792392	28.193010073210722	28.198739285975314	21.73073773289004
98-99	22.078128753798445	28.966150713762488	27.22689125820821	21.72882927423086
100	20.25891158094297	28.6492357687196	28.292287435213247	22.799565215124183
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2684.0
1	2228.5
2	2709.5
3	3872.5
4	5172.0
5	8478.5
6	11504.5
7	14028.0
8	16133.0
9	16206.0
10	14736.5
11	13149.5
12	13870.0
13	18016.0
14	28302.0
15	42558.5
16	54241.5
17	61375.0
18	62865.0
19	59119.0
20	53037.5
21	46354.0
22	41498.0
23	41620.0
24	46306.5
25	55068.0
26	68123.0
27	83304.0
28	99719.5
29	117573.5
30	135312.5
31	153625.5
32	174086.5
33	193969.5
34	213410.0
35	231471.5
36	249905.5
37	267191.5
38	280015.5
39	291653.5
40	304151.0
41	316690.5
42	325881.5
43	333047.0
44	338123.5
45	342407.0
46	345270.5
47	343062.5
48	337542.5
49	327117.0
50	312296.5
51	294822.5
52	273326.5
53	251458.0
54	230567.5
55	207162.5
56	183969.5
57	161020.5
58	138238.5
59	118383.5
60	97703.0
61	78701.0
62	62445.0
63	47665.5
64	35443.5
65	25848.5
66	19435.0
67	15008.0
68	11501.0
69	8383.0
70	5992.5
71	4380.0
72	3094.0
73	2086.5
74	1397.5
75	955.0
76	677.0
77	462.0
78	306.5
79	228.5
80	161.0
81	112.5
82	89.0
83	66.5
84	50.5
85	40.5
86	34.0
87	29.0
88	18.0
89	8.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.197254544243684E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.006756430907516789
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.0224318849638245E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0016823179936906393
36-37	0.03696172097375907
38-39	0.11761507465252005
40-41	0.15336047981654072
42-43	0.05431446914942458
44-45	0.001477354504379707
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.008965425986826182
64-65	0.07077218901488708
66-67	0.16964089366336563
68-69	0.18676580607409146
70-71	0.07257678941025565
72-73	0.004784609910753976
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	103.0
20-21	478.0
22-23	1019.0
24-25	2290.0
26-27	3778.0
28-29	6103.0
30-31	11248.0
32-33	12344.0
34-35	16022.0
36-37	22662.0
38-39	25657.0
40-41	35848.0
42-43	36951.0
44-45	43052.0
46-47	49667.0
48-49	41902.0
50-51	39997.0
52-53	32454.0
54-55	37250.0
56-57	39517.0
58-59	38536.0
60-61	21936.0
62-63	37223.0
64-65	49895.0
66-67	62565.0
68-69	58970.0
70-71	60150.0
72-73	57672.0
74-75	55435.0
76-77	52792.0
78-79	57977.0
80-81	59332.0
82-83	60978.0
84-85	64215.0
86-87	71191.0
88-89	77579.0
90-91	80435.0
92-93	86653.0
94-95	102721.0
96-97	285114.0
98-99	45241.0
100-101	6328647.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.9995394341281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.6383514747629	72.73942809171561
2	8.551091326947056	14.194732835915454
3	2.2032105894356797	5.485963925986658
4	0.7811058316768638	2.5932569711396507
5	0.3550444297258544	1.4734262072949287
6	0.17532718585428841	0.8731245405712631
7	0.09361959122641407	0.5439268067662594
8	0.062235380500832625	0.4132406334461459
9	0.036471883945062164	0.2724434612761617
>10	0.10152394376067352	1.21554678358189
>50	0.001556829317345385	0.08966251537154857
>100	3.910557344367284E-4	0.07063737615056925
>500	7.047711253128909E-5	0.034609850783797386
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2086638474985312E-5
2	0.0	3.625991542495594E-5	0.0	1.2086638474985312E-5	1.2086638474985312E-5
3	0.0	3.625991542495594E-5	0.0	1.2086638474985312E-5	1.2086638474985312E-5
4	0.0	7.251983084991188E-5	0.0	1.2086638474985312E-5	1.2086638474985312E-5
5	0.0	7.251983084991188E-5	0.0	1.2086638474985312E-5	1.2086638474985312E-5
6	1.5712630017480905E-4	7.251983084991188E-5	0.0	1.2086638474985312E-5	1.2086638474985312E-5
7	1.5712630017480905E-4	7.251983084991188E-5	0.0	1.2086638474985312E-5	1.2086638474985312E-5
8	1.5712630017480905E-4	7.251983084991188E-5	0.0	1.2086638474985312E-5	1.2086638474985312E-5
9	1.5712630017480905E-4	9.66931077998825E-5	0.0	1.2086638474985312E-5	4.834655389994125E-5
10-11	1.631696194123017E-4	9.66931077998825E-5	0.0	6.043319237492656E-5	4.834655389994125E-5
12-13	1.6921293864979436E-4	1.0273642703737515E-4	0.0	6.647651161241922E-5	4.834655389994125E-5
14-15	1.812995771247797E-4	1.5712630017480905E-4	0.0	8.460646932489718E-5	5.4389873137433906E-5
16-17	2.7799268492466214E-4	1.5712630017480905E-4	0.0	1.087797462748678E-4	6.043319237492656E-5
18-19	2.7799268492466214E-4	1.6921293864979436E-4	0.0	1.087797462748678E-4	1.0273642703737516E-4
20-21	2.7799268492466214E-4	1.812995771247797E-4	0.0	1.3295302322483842E-4	1.4503966169982375E-4
22-23	2.840360041621548E-4	2.5381940797469154E-4	0.0	1.4503966169982375E-4	1.4503966169982375E-4
24-25	2.900793233996475E-4	2.961226426371401E-4	0.0	1.6921293864979436E-4	1.5712630017480905E-4
26-27	2.900793233996475E-4	3.142526003496181E-4	0.0	2.054728540747503E-4	1.631696194123017E-4
28-29	2.900793233996475E-4	3.142526003496181E-4	0.0	4.8346553899941247E-4	1.8129957712477966E-4
30-31	2.900793233996475E-4	3.988590696745153E-4	0.0	0.0011844905705485605	1.93386215599765E-4
32-33	2.900793233996475E-4	4.049023889120079E-4	0.0	0.0028101434454340848	1.93386215599765E-4
34-35	3.142526003496181E-4	4.955521774743978E-4	0.0	0.00445996959726958	1.93386215599765E-4
36-37	3.2029591958711073E-4	5.80158646799295E-4	0.0	0.0069860770385415105	1.93386215599765E-4
38-39	3.263392388246034E-4	5.922452852742803E-4	0.0	0.010726891646549464	1.93386215599765E-4
40-41	3.384258772995887E-4	6.526784776492068E-4	0.0	0.014649005831682198	1.93386215599765E-4
42-43	3.384258772995887E-4	6.768517545991774E-4	0.0	0.01877659287088968	1.93386215599765E-4
44-45	3.384258772995887E-4	7.37284946974104E-4	0.0	0.022233371474735482	1.93386215599765E-4
46-47	3.384258772995887E-4	7.856315008740452E-4	0.0	0.025490720543744023	2.054728540747503E-4
48-49	3.384258772995887E-4	7.91674820111538E-4	0.0	0.029068365532339675	2.054728540747503E-4
50-51	3.5051251577457407E-4	8.883679279114204E-4	0.0	0.03312343274069725	2.054728540747503E-4
52-53	3.625991542495594E-4	8.944112471489131E-4	0.0	0.03754109910330438	2.054728540747503E-4
54-55	3.625991542495594E-4	9.004545663864057E-4	0.0	0.04059901863747566	2.054728540747503E-4
56-57	3.625991542495594E-4	9.185845240988837E-4	0.0	0.04322181918654748	2.054728540747503E-4
58-59	3.625991542495594E-4	9.185845240988837E-4	0.0	0.04612261242054395	2.054728540747503E-4
60-61	4.049023889120079E-4	9.427578010488542E-4	0.0	0.04823777415366638	2.175594925497356E-4
62-63	4.7137890052442716E-4	9.608877587613323E-4	0.0	0.04988760030550188	2.175594925497356E-4
64-65	4.7137890052442716E-4	0.001087797462748678	0.0	0.05240766442753631	2.175594925497356E-4
66-67	4.7742221976191984E-4	0.001087797462748678	0.0	0.05639021180504397	2.2360281178722825E-4
68-69	4.8346553899941247E-4	0.0011361440166486193	0.0	0.060838094763838564	2.2964613102472093E-4
70-71	4.955521774743978E-4	0.0011663606128360826	0.0	0.0646816457988839	2.4173276949970623E-4
72-73	5.559853698493243E-4	0.0011724039320735753	0.0	0.0681323810834922	2.4173276949970623E-4
74-75	5.559853698493243E-4	0.0011784472513110679	0.0	0.07087604801731387	2.4173276949970623E-4
76-77	5.80158646799295E-4	0.001208663847498531	0.0	0.07323898583917349	2.4173276949970623E-4
78-79	5.922452852742803E-4	0.0012328371244485017	0.0	0.07481024884092158	2.4173276949970623E-4
80-81	5.922452852742803E-4	0.0012328371244485017	0.0	0.07544479736085832	2.477760887371989E-4
82-83	5.982886045117729E-4	0.0012509670821609798	0.0	0.07572883336502048	2.5381940797469154E-4
84-85	6.043319237492656E-4	0.0012872269975859358	0.0	0.0759403495383327	2.5986272721218416E-4
86-87	6.043319237492656E-4	0.0012932703168234284	0.0	0.07601286936918263	2.6590604644967684E-4
88	6.043319237492656E-4	0.0013416168707233696	0.0	0.07601286936918263	2.7799268492466214E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13910	0.0	46.861427	1
GTATCAA	25125	0.0	37.035336	1
TCAACGC	29235	0.0	31.407751	4
ATCAACG	29455	0.0	31.113476	3
CAACGCA	29930	0.0	30.649063	5
TATCAAC	30475	0.0	30.187489	2
AACGCAG	30770	0.0	29.872393	6
CGCAGAG	34930	0.0	26.31489	8
ACGCAGA	34975	0.0	26.268463	7
GCAGAGT	40255	0.0	22.76839	9
GTACATG	23990	0.0	21.45498	1
GAGTACT	26480	0.0	21.339252	12-13
TACATGG	23270	0.0	21.268824	2
ACATGGG	23385	0.0	20.431189	3
GTGGTAT	7350	0.0	19.973797	1
AGAGTAC	35835	0.0	19.81122	10-11
CAGAGTA	39280	0.0	19.78595	10-11
TGGTATC	7110	0.0	19.473429	2
GTACTTT	29490	0.0	19.145645	14-15
CATGGGG	16325	0.0	18.44331	4
>>END_MODULE