FastQCFastQC Report
Fri 27 May 2016
SRR2139153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9521476
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143850.051.2722661
GTATCAA266250.037.8549271
ATCAACG324800.030.3865723
TCAACGC326000.030.353324
TATCAAC331450.029.9961132
CAACGCA332900.029.724195
AACGCAG340900.029.1173936
CTTATAC66600.028.0866491
ACGCAGA387450.025.5641127
GTGGTAT69550.025.4830281
CGCAGAG390500.025.4081958
TGGTATC65650.025.1103422
GCAGAGT449450.021.9711179
TATACAC108050.021.6597563
GAGTACT303300.020.340812-13
GTACATG271900.020.2147981
TACATGG267350.019.839992
CAGAGTA440150.019.43685210-11
ACATGGG269950.019.174193
AGAGTAC413900.018.99267210-11
Produced by FastQC (version 0.11.5)