FastQCFastQC Report
Fri 27 May 2016
SRR2139152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8418527
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149550.046.1522561
GTATCAA260950.035.626881
CTTATAC76000.030.3086411
ATCAACG309050.029.4371873
TATCAAC333350.027.8142412
TCAACGC330500.027.577064
ACGCAGA329900.027.5679157
AACGCAG332800.027.3019336
CAACGCA333500.027.2675785
TATACAC94900.025.7592723
CGCAGAG380150.023.8804658
GCAGAGT422800.021.4518859
ACACATC106800.021.220346
ACATGGG267200.020.7828063
TACATGG276600.020.6166972
GAGTACT277250.020.02351412-13
GTGGTAT76250.019.5952031
GTACATG299700.019.5539761
TGGTATC75750.018.7382052
CAGAGTA424500.018.69150710-11
Produced by FastQC (version 0.11.5)