##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139152_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8418527
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70128325299663	33.0	33.0	33.0	33.0	33.0
2	32.77918904340391	33.0	33.0	33.0	33.0	33.0
3	33.51127269651805	33.0	33.0	33.0	33.0	37.0
4	36.61144354588398	37.0	37.0	37.0	37.0	37.0
5	36.625578916596695	37.0	37.0	37.0	37.0	37.0
6	36.599723918447964	37.0	37.0	37.0	37.0	37.0
7	36.59168617027658	37.0	37.0	37.0	37.0	37.0
8	36.56996906941084	37.0	37.0	37.0	37.0	37.0
9	36.60641392490634	37.0	37.0	37.0	37.0	37.0
10-11	36.59916420057809	37.0	37.0	37.0	37.0	37.0
12-13	36.84095685622913	37.0	37.0	37.0	37.0	38.5
14-15	38.669100841513014	40.0	37.0	40.0	37.0	40.0
16-17	38.60527031629167	40.0	37.0	40.0	37.0	40.0
18-19	38.636952224539996	40.0	37.0	40.0	37.0	40.0
20-21	38.649605853099004	40.0	37.0	40.0	37.0	40.0
22-23	38.64896996991058	40.0	37.0	40.0	37.0	40.0
24-25	38.63924879740591	40.0	37.0	40.0	37.0	40.0
26-27	38.55836031646829	40.0	37.0	40.0	37.0	40.0
28-29	38.44547722441912	40.0	37.0	40.0	37.0	40.0
30-31	38.30144322686833	40.0	37.0	40.0	37.0	40.0
32-33	38.18154513653445	40.0	37.0	40.0	37.0	40.0
34-35	38.145351328795044	40.0	37.0	40.0	37.0	40.0
36-37	38.1307378101189	40.0	37.0	40.0	37.0	40.0
38-39	38.08261311310337	40.0	37.0	40.0	37.0	40.0
40-41	38.076487388890556	40.0	37.0	40.0	37.0	40.0
42-43	38.03477088784872	40.0	37.0	40.0	35.0	40.0
44-45	37.93138573168795	40.0	37.0	40.0	33.0	40.0
46-47	37.85492737705779	40.0	37.0	40.0	33.0	40.0
48-49	37.805718177923524	40.0	37.0	40.0	33.0	40.0
50-51	37.74943392402127	40.0	37.0	40.0	33.0	40.0
52-53	37.70916564166582	40.0	37.0	40.0	33.0	40.0
54-55	37.59973926358727	40.0	37.0	40.0	33.0	40.0
56-57	37.418869201059294	40.0	37.0	40.0	33.0	40.0
58-59	37.21174533408619	37.0	37.0	40.0	33.0	40.0
60-61	37.06980602577436	37.0	37.0	40.0	33.0	40.0
62-63	36.95666626965872	37.0	37.0	40.0	33.0	40.0
64-65	36.79126137648926	37.0	37.0	40.0	33.0	40.0
66-67	36.59709549095572	37.0	37.0	40.0	33.0	40.0
68-69	36.39852146033108	37.0	37.0	40.0	33.0	40.0
70-71	36.17855030576861	37.0	37.0	38.5	33.0	40.0
72-73	35.870066425191126	37.0	37.0	37.0	33.0	40.0
74-75	35.26361368102757	37.0	33.0	37.0	30.0	40.0
76-77	33.581299169382895	35.0	33.0	37.0	27.0	37.0
78-79	35.13508765111405	37.0	33.0	37.0	33.0	37.0
80-81	35.32331222396837	37.0	33.0	37.0	33.0	37.0
82-83	35.17320380137019	37.0	33.0	37.0	33.0	37.0
84-85	35.01632766475082	37.0	33.0	37.0	33.0	37.0
86-87	34.89689912791731	37.0	33.0	37.0	33.0	37.0
88-89	34.8021535658309	37.0	33.0	37.0	33.0	37.0
90-91	34.729378239750396	37.0	33.0	37.0	33.0	37.0
92-93	34.6605265449775	37.0	33.0	37.0	33.0	37.0
94-95	34.66542748915499	37.0	33.0	37.0	33.0	37.0
96-97	34.696670611650504	37.0	33.0	37.0	33.0	37.0
98-99	34.69151367206666	37.0	33.0	37.0	33.0	37.0
100	34.6010065927513	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	11.0
20	42.0
21	212.0
22	823.0
23	2474.0
24	5832.0
25	11480.0
26	20830.0
27	34167.0
28	52033.0
29	75370.0
30	104347.0
31	142712.0
32	195347.0
33	267065.0
34	379062.0
35	568733.0
36	1024132.0
37	2493947.0
38	2921096.0
39	118810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.89728477150016	0.02073999247848612	25.322034115743413	29.75994112027794
2	14.384342213608397	23.726163828554895	39.98562453109268	21.903869426744027
3	16.506286254862193	24.803475097659614	33.26160660429153	25.428632043186656
4	12.519577355991137	23.47332258956941	38.04473157833906	25.96236847610039
5	16.85471817100545	36.55676343379311	32.63284657755448	13.955671817646959
6	27.117214210989644	37.81825490373791	20.548796719426093	14.515734165846354
7	25.075289299422572	33.38453389767592	23.14997623693551	18.390200565965994
8	23.3746026323373	32.758727596731305	22.66292222652754	21.203747544403853
9	23.37014662200639	21.101113046263013	24.22464381498026	31.304096516750334
10-11	24.574937804721525	26.363373898824182	28.669761973248146	20.391926323206146
12-13	24.673979684144264	24.264319715579447	28.83538131639304	22.22631928388325
14-15	21.960203356916715	26.541554498016808	27.24081810783609	24.25742403723039
16-17	20.881158901076162	28.946732605359582	28.30502889638532	21.867079597178936
18-19	20.820358478389387	27.896477614195454	30.286361260111182	20.996802647303976
20-21	21.83818407938776	27.43466877509533	30.035510241972958	20.691636903543962
22-23	21.418110183555306	27.070448661312795	30.037583770786995	21.473857384344903
24-25	21.125868376185476	27.33099768120113	30.38132118361704	21.16181275899636
26-27	21.192950890624594	27.751330257818545	29.984569330257106	21.071149521299755
28-29	20.985449553314474	27.568995678303626	30.186709250573866	21.25884551780803
30-31	21.31528067855873	27.670173500329348	29.984643204737253	21.02990261637467
32-33	21.398676765094915	27.7295708120196	29.803836585376636	21.067915837508846
34-35	21.165665145031912	27.59074150665831	29.990794861435916	21.25279848687386
36-37	21.27589303248176	27.79639443247028	29.89658227170151	21.031130263346455
38-39	21.067096263740005	27.738907878368806	29.98926919541467	21.204726662476514
40-41	21.48644861561066	27.67822046594171	29.607498287565175	21.22783263088245
42-43	21.639135925569807	27.495948465206737	29.54577382939369	21.31914177982976
44-45	21.408221059973105	28.30543417074436	28.985991324997922	21.300353444284607
46-47	21.569691279704976	27.794585420629364	28.55049955421437	22.085223745451284
48-49	21.69303148050832	27.80454108985645	28.72919551516872	21.77323191446651
50-51	21.37043489221276	28.123601005851974	28.448379121707557	22.05758498022771
52-53	21.59396279749453	28.017741037008303	28.497650177373878	21.89064598812329
54-55	21.684080792104634	28.09057612483572	28.307068358618388	21.918274724441254
56-57	21.925905097445504	28.539946236091186	27.733560690568364	21.800587975894942
58-59	21.6218067039554	28.47933961200715	28.374826339922656	21.5240273441148
60-61	21.796798476676486	28.535933057712633	27.876212181198884	21.791056284412004
62-63	21.750783548356612	28.57531490343323	27.844929588223028	21.828971959987133
64-65	21.618058145291865	28.500191181546384	27.8910828872662	21.99066778589555
66-67	21.8096862565726	28.47934173987262	27.6852086574648	22.02576334608998
68-69	21.928534890177552	28.305125645778716	27.919342884407765	21.846996579635963
70-71	21.918964429601168	27.686359585363274	28.072101790424455	22.322574194611104
72-73	21.878296806135427	27.93730914996624	28.039834665615153	22.14455937828318
74-75	21.666172720071575	27.993599216189775	28.16863557158976	22.171592492148896
76-77	21.894062621044302	27.992111178968532	27.69838938303348	22.415436816953687
78-79	21.82120772949398	28.02879497657505	27.859562448529328	22.290434845401638
80-81	21.811540777121284	27.982732326368907	27.91385915032866	22.29186774618115
82-83	22.118067131961638	27.622165682595814	28.083460667927955	22.176306517514597
84-85	22.488613192035327	27.54133469242278	27.931715089673297	22.038337025868593
86-87	21.836660710906088	27.857982738410207	27.93644522677464	22.368911323909057
88-89	22.119840912009796	27.65055085344178	28.011212993945193	22.218395240603233
90-91	22.318060118029283	27.62434996087297	27.96368374701958	22.093906174078167
92-93	22.129626451047287	27.638984979878106	27.976272604121554	22.25511596495305
94-95	22.2731031929799	27.487304904336234	27.85393174259314	22.385660160090723
96-97	22.018247827984208	27.93296238619451	28.260600188472306	21.78818959734898
98-99	23.241263265899935	29.899338044993033	27.622991929432306	19.236406759674725
100	22.54114771286603	30.271311860176013	21.805386318470177	25.382154108487782
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1073.0
1	1133.0
2	2658.0
3	4372.0
4	6418.0
5	12207.0
6	16837.0
7	18689.0
8	18856.0
9	16959.5
10	14575.5
11	12181.0
12	12699.5
13	16888.0
14	28302.5
15	47267.5
16	65205.5
17	76626.0
18	79053.0
19	73766.5
20	64957.5
21	55419.5
22	48657.0
23	47371.5
24	51757.5
25	62518.5
26	80912.5
27	101067.0
28	115815.0
29	131974.5
30	146864.5
31	160565.0
32	181353.0
33	205230.0
34	232793.0
35	258500.0
36	285986.5
37	312855.0
38	320309.5
39	322583.0
40	333869.5
41	339971.0
42	342234.0
43	350817.0
44	359183.5
45	366539.0
46	374432.5
47	381010.0
48	381778.0
49	374622.5
50	363526.0
51	344921.5
52	318293.5
53	290657.5
54	264017.0
55	236215.5
56	207406.5
57	178619.5
58	151329.0
59	128833.5
60	106168.5
61	80343.5
62	59081.0
63	43241.5
64	30317.0
65	21202.0
66	15197.0
67	10912.5
68	7889.0
69	5718.0
70	4493.5
71	3540.0
72	2671.5
73	1900.5
74	1310.0
75	870.0
76	625.0
77	438.0
78	296.0
79	208.5
80	129.5
81	68.5
82	50.5
83	32.5
84	19.0
85	14.0
86	11.5
87	10.0
88	7.0
89	4.5
90	2.5
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0690706343283094E-4
2	2.2569268946930976E-4
3	1.1878562603647884E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	1.1878562603647884E-5
9	1.0690706343283094E-4
10-11	9.799814148009503E-4
12-13	0.006770780684079293
14-15	3.3259975290214074E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.158020706111512E-5
24-25	4.752973980378654E-5
26-27	5.943559365905426E-6
28-29	1.7843745178471356E-5
30-31	2.9788932899058358E-5
32-33	3.106904892980869E-4
34-35	0.005601458058333128
36-37	0.04428038229553963
38-39	0.1425111475939865
40-41	0.1641667184502515
42-43	0.04839535184699557
44-45	0.005577002415535268
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	6.5781112558587944E-6
56-57	1.993273366811467E-5
58-59	6.713766423635036E-5
60-61	0.0016832620695829317
62-63	0.01908193457469517
64-65	0.09321295915358194
66-67	0.20327997587968036
68-69	0.19960042951055385
70-71	0.07386128482696865
72-73	0.004752516344768465
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	123.0
20-21	627.0
22-23	1465.0
24-25	2802.0
26-27	5111.0
28-29	10657.0
30-31	22495.0
32-33	29149.0
34-35	39558.0
36-37	56429.0
38-39	66324.0
40-41	70391.0
42-43	83494.0
44-45	95410.0
46-47	92479.0
48-49	80869.0
50-51	71028.0
52-53	70768.0
54-55	74607.0
56-57	77734.0
58-59	79760.0
60-61	79893.0
62-63	78566.0
64-65	82537.0
66-67	92718.0
68-69	77921.0
70-71	76478.0
72-73	75296.0
74-75	73352.0
76-77	70534.0
78-79	77687.0
80-81	79549.0
82-83	74658.0
84-85	83196.0
86-87	89054.0
88-89	95736.0
90-91	96462.0
92-93	102021.0
94-95	124873.0
96-97	380017.0
98-99	1418454.0
100-101	4058245.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.14017415188007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.19516223642474	66.4910982596736
2	8.312873256292859	12.825129813858748
3	2.6633344541701165	6.163502508581557
4	1.1604346385964102	3.5806452045280444
5	0.5971710535150871	2.3032939533307752
6	0.32941564370024823	1.5246708074034494
7	0.20987827936218392	1.1333032914487062
8	0.13964145976132997	0.8617573219849397
9	0.09035758761956221	0.6273180040425111
>10	0.2956434703625874	3.3213339541754925
>50	0.003264663957070404	0.16973066651986174
>100	0.002094817920617288	0.31668563339427275
>500	3.642191957741907E-4	0.19708018364255347
>1k	3.6421912146796746E-4	0.48445039741548046
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.3757125207295764E-5	0.0	0.0	0.0
3	0.0	2.3757125207295764E-5	0.0	0.0	0.0
4	0.0	2.3757125207295764E-5	0.0	0.0	0.0
5	0.0	2.3757125207295764E-5	0.0	0.0	0.0
6	0.0	2.3757125207295764E-5	0.0	0.0	0.0
7	0.0	2.3757125207295764E-5	0.0	0.0	0.0
8	0.0	3.563568781094365E-5	0.0	0.0	0.0
9	0.0	8.314993822553519E-5	0.0	1.1878562603647882E-5	0.0
10-11	0.0	8.314993822553519E-5	0.0	1.1878562603647882E-5	0.0
12-13	0.0	8.314993822553519E-5	0.0	1.1878562603647882E-5	0.0
14-15	0.0	1.6629987645107037E-4	0.0	1.7817843905471822E-5	0.0
16-17	0.0	1.900570016583661E-4	0.0	2.3757125207295764E-5	0.0
18-19	0.0	1.900570016583661E-4	0.0	2.969640650911971E-5	0.0
20-21	0.0	1.900570016583661E-4	0.0	4.157496911276759E-5	0.0
22-23	0.0	2.1381412686566188E-4	0.0	4.751425041459153E-5	0.0
24-25	0.0	2.7914622118572525E-4	0.0	5.345353171641547E-5	0.0
26-27	0.0	2.9696406509119707E-4	0.0	1.3066418864012672E-4	0.0
28-29	0.0	2.9696406509119707E-4	0.0	4.3950681633497167E-4	0.0
30-31	0.0	3.444783155057886E-4	0.0	0.001407609668532274	0.0
32-33	0.0	3.444783155057886E-4	0.0	0.0035813866249998364	0.0
34-35	0.0	3.860532846185562E-4	0.0	0.006758902121475645	0.0
36-37	0.0	4.2762825373132376E-4	0.0	0.011148031003523538	0.0
38-39	0.0	4.2762825373132376E-4	0.0	0.017651544029020756	0.0
40-41	0.0	4.513853789386195E-4	0.0	0.02409566424149973	0.0
42-43	0.0	5.345353171641548E-4	0.0	0.03069420576782613	0.0
44-45	0.0	6.1768525538969E-4	0.0	0.038076732425993284	0.0
46-47	0.0	6.1768525538969E-4	0.0	0.045411744833745854	0.0
48-49	0.0	6.1768525538969E-4	0.0	0.053168446213927925	0.0
50-51	0.0	6.889566310115772E-4	0.0	0.061311200878728545	0.0
52-53	0.0	6.889566310115772E-4	0.0	0.06889566310115772	0.0
54-55	0.0	6.948959123134011E-4	0.0	0.07558923312831331	0.0
56-57	0.0	7.008351936152251E-4	0.0	0.08074452929829648	0.0
58-59	5.939281301823941E-6	7.008351936152251E-4	0.0	0.08629181803420005	0.0
60-61	1.1878562603647882E-5	7.008351936152251E-4	0.0	0.09169062473755801	0.0
62-63	1.1878562603647882E-5	7.008351936152251E-4	0.0	0.09712506712872691	0.0
64-65	1.1878562603647882E-5	7.780458505389364E-4	0.0	0.10264859873942318	0.0
66-67	1.1878562603647882E-5	7.839851318407603E-4	0.0	0.10852848722822889	0.0
68-69	1.1878562603647882E-5	8.1368153834988E-4	0.0	0.11408171524543427	0.0
70-71	1.1878562603647882E-5	8.314993822553518E-4	0.0	0.11914198291458826	0.0
72-73	1.1878562603647882E-5	8.314993822553518E-4	0.0	0.12317475491852672	0.0
74-75	1.1878562603647882E-5	8.314993822553518E-4	0.0	0.12665517376139557	0.0
76-77	1.1878562603647882E-5	8.493172261608236E-4	0.0	0.12983268925787136	0.0
78-79	1.1878562603647882E-5	8.552565074626475E-4	0.0	0.13166198789883315	0.0
80-81	1.1878562603647882E-5	8.611957887644714E-4	0.0	0.13219058393469546	0.0
82-83	1.1878562603647882E-5	8.671350700662954E-4	0.0	0.13260633362582314	0.0
84-85	1.1878562603647882E-5	8.671350700662954E-4	0.0	0.13282608703399063	0.0
86-87	1.1878562603647882E-5	8.671350700662954E-4	0.0	0.13295081194132893	0.0
88	1.1878562603647882E-5	9.14649320480887E-4	0.0	0.13295675122263076	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14955	0.0	46.152256	1
GTATCAA	26095	0.0	35.62688	1
CTTATAC	7600	0.0	30.308641	1
ATCAACG	30905	0.0	29.437187	3
TATCAAC	33335	0.0	27.814241	2
TCAACGC	33050	0.0	27.57706	4
ACGCAGA	32990	0.0	27.567915	7
AACGCAG	33280	0.0	27.301933	6
CAACGCA	33350	0.0	27.267578	5
TATACAC	9490	0.0	25.759272	3
CGCAGAG	38015	0.0	23.880465	8
GCAGAGT	42280	0.0	21.451885	9
ACACATC	10680	0.0	21.22034	6
ACATGGG	26720	0.0	20.782806	3
TACATGG	27660	0.0	20.616697	2
GAGTACT	27725	0.0	20.023514	12-13
GTGGTAT	7625	0.0	19.595203	1
GTACATG	29970	0.0	19.553976	1
TGGTATC	7575	0.0	18.738205	2
CAGAGTA	42450	0.0	18.691507	10-11
>>END_MODULE