FastQCFastQC Report
Fri 27 May 2016
SRR2139151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14864597
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA244200.051.1971051
GTATCAA459350.039.2098431
ATCAACG568200.031.1125563
TCAACGC573700.030.8365964
CAACGCA580000.030.4575085
TATCAAC589550.030.3695452
AACGCAG598250.029.6237956
CTTATAC154900.028.9517481
ACGCAGA670650.026.3326157
CGCAGAG679400.025.993658
TATACAC198650.025.367833
GCAGAGT759200.023.1265289
TACATGG482000.021.3261872
GAGTACT508000.020.63228212-13
ACATGGG490400.020.2995433
GTACATG534400.019.8898141
CAGAGTA751900.019.84612810-11
AGAGTAC718200.019.5651310-11
ACACATC240850.019.1000066
TTATACA259300.018.8090632
Produced by FastQC (version 0.11.5)