##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139151_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14864597
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.596448528002476	33.0	33.0	33.0	33.0	33.0
2	32.6306663409711	33.0	33.0	33.0	33.0	33.0
3	32.66654218745386	33.0	33.0	33.0	33.0	33.0
4	36.593123715362076	37.0	37.0	37.0	37.0	37.0
5	36.6072949707281	37.0	37.0	37.0	37.0	37.0
6	36.58654849505842	37.0	37.0	37.0	37.0	37.0
7	36.565027427248786	37.0	37.0	37.0	37.0	37.0
8	36.54634094688205	37.0	37.0	37.0	37.0	37.0
9	36.58142410453509	37.0	37.0	37.0	37.0	37.0
10-11	36.555862933922796	37.0	37.0	37.0	37.0	37.0
12-13	36.54876223687732	37.0	37.0	37.0	37.0	37.0
14-15	38.620969071680854	40.0	37.0	40.0	37.0	40.0
16-17	38.55544765189396	40.0	37.0	40.0	37.0	40.0
18-19	38.58565196890302	40.0	37.0	40.0	37.0	40.0
20-21	38.59654709996772	40.0	37.0	40.0	37.0	40.0
22-23	38.58854748953719	40.0	37.0	40.0	37.0	40.0
24-25	38.58696735325647	40.0	37.0	40.0	37.0	40.0
26-27	38.50879776993621	40.0	37.0	40.0	37.0	40.0
28-29	38.39018855568244	40.0	37.0	40.0	37.0	40.0
30-31	38.24571837371429	40.0	37.0	40.0	37.0	40.0
32-33	38.12097554658571	40.0	37.0	40.0	37.0	40.0
34-35	38.06606965222791	40.0	37.0	40.0	35.0	40.0
36-37	38.04503729589342	40.0	37.0	40.0	33.0	40.0
38-39	37.990019888667035	40.0	37.0	40.0	33.0	40.0
40-41	37.97317073449919	40.0	37.0	40.0	33.0	40.0
42-43	37.934313097582596	40.0	37.0	40.0	33.0	40.0
44-45	37.823929277831255	40.0	37.0	40.0	33.0	40.0
46-47	37.740551695158125	40.0	37.0	40.0	33.0	40.0
48-49	37.66386212673112	40.0	37.0	40.0	33.0	40.0
50-51	37.606315344578285	40.0	37.0	40.0	33.0	40.0
52-53	37.54713230499023	40.0	37.0	40.0	33.0	40.0
54-55	37.432810611749986	40.0	37.0	40.0	33.0	40.0
56-57	37.250110025832356	38.5	37.0	40.0	33.0	40.0
58-59	37.00894498154534	37.0	37.0	40.0	33.0	40.0
60-61	36.84956595974419	37.0	37.0	40.0	33.0	40.0
62-63	36.72175528153912	37.0	37.0	40.0	33.0	40.0
64-65	36.55249221506472	37.0	37.0	40.0	33.0	40.0
66-67	36.348861973639686	37.0	37.0	40.0	33.0	40.0
68-69	36.14903115619869	37.0	37.0	40.0	33.0	40.0
70-71	35.93651963600573	37.0	37.0	38.5	33.0	40.0
72-73	35.646412650788335	37.0	33.0	37.0	33.0	40.0
74-75	35.310888346007246	37.0	33.0	37.0	33.0	40.0
76-77	33.01642394623664	33.0	33.0	37.0	27.0	37.0
78-79	34.83774151506509	37.0	33.0	37.0	30.0	37.0
80-81	35.11026695367549	37.0	33.0	37.0	33.0	37.0
82-83	34.97219804635654	37.0	33.0	37.0	33.0	37.0
84-85	34.80009022037354	37.0	33.0	37.0	33.0	37.0
86-87	34.68148957958118	37.0	33.0	37.0	33.0	37.0
88-89	34.57359673787104	37.0	33.0	37.0	33.0	37.0
90-91	34.50269483887615	37.0	33.0	37.0	33.0	37.0
92-93	34.432496937560686	37.0	33.0	37.0	33.0	37.0
94-95	34.42666593565944	37.0	33.0	37.0	33.0	37.0
96-97	34.47198479334353	37.0	33.0	37.0	33.0	37.0
98-99	34.488521187092225	37.0	33.0	37.0	33.0	37.0
100	34.41678918232526	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	3.0
18	4.0
19	11.0
20	103.0
21	467.0
22	1792.0
23	5658.0
24	13246.0
25	26946.0
26	47235.0
27	76302.0
28	112383.0
29	158171.0
30	212617.0
31	282452.0
32	373059.0
33	504528.0
34	703463.0
35	1056048.0
36	1928067.0
37	4582179.0
38	4639538.0
39	140324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.28502660180113	20.643690595001075	14.61557479366239	23.45570800953541
2	14.26556280613505	20.49071216979932	43.050607824961375	22.193117199104258
3	17.954594931473412	26.677004877899137	30.625474113120866	24.74292607750658
4	11.953422750271853	17.949892482782577	39.489233343442365	30.6074514235032
5	12.793364851624625	38.06893238134423	35.82151173163401	13.316191035397127
6	29.149968152514877	38.50903179608783	18.667301822397324	13.673698228999967
7	25.878120064615278	33.14697553838665	23.17125874123992	17.803645655758153
8	24.0839576131097	36.869128498346	21.230748551793805	17.81616533675049
9	25.251900556194062	17.039750020787647	21.627308269932126	36.08104115308616
10-11	24.137196588646166	27.162128916108525	28.641112840126105	20.059561655119207
12-13	24.93185998950181	24.386289137279384	28.94452819165105	21.737322681567765
14-15	22.037874725941553	25.748432678328882	27.125035234884653	25.088657360844913
16-17	21.19394155119039	28.979494028664217	27.634509701137546	22.192054719007857
18-19	21.03457631579248	27.758091928089275	30.004234221755222	21.203097534363025
20-21	22.221474713940168	27.09708589726805	29.78065146871698	20.9007879200748
22-23	21.63764828065739	27.074058774985737	29.736082203851876	21.552210740504993
24-25	21.34597863197954	27.169535889195867	30.1543812678602	21.330104210964393
26-27	21.41991961708136	27.47030422636868	29.82939287440104	21.280383282148918
28-29	21.137748169338373	27.600105962099086	29.85435330317917	21.40779256538337
30-31	21.854339895472048	27.376482818679886	29.694395043427768	21.0747822424203
32-33	21.63752969222268	27.55119628668089	29.634668791088835	21.176605230007592
34-35	21.45856023017398	27.670276179300302	29.75816248414157	21.113001106384147
36-37	21.740581368178503	27.24563233524882	29.69626386809289	21.317522428479787
38-39	21.340499433898067	27.43151284473136	29.81403981341455	21.41394790795602
40-41	21.725390973976918	27.420533646059603	29.561002289475415	21.29307309048807
42-43	21.789549365759495	27.407628906173542	29.34541541338595	21.457406314681013
44-45	21.64775830772707	27.92958282622957	28.899927655397544	21.522731210645812
46-47	21.953411969065677	27.476718282972453	28.380702648046757	22.189167099915117
48-49	21.96323440133132	27.600107832171684	28.467311792075385	21.969345974421618
50-51	21.563112631457983	27.829080872188083	28.447621752591047	22.160184743762894
52-53	21.873350219455688	27.902944903875294	28.265864432784525	21.9578404438845
54-55	22.031515507629308	27.758353638443367	28.107412361688848	22.102718492238473
56-57	22.163507123096807	28.027905389342628	27.749690582346286	22.058896905214272
58-59	21.78994431337157	28.28113842775789	28.2293282422351	21.69958901663544
60-61	21.948520748340574	28.418888188224905	27.742300011480147	21.890291051954375
62-63	21.831297416995245	28.44421300220006	27.66813333646142	22.056356244343274
64-65	21.82491381715449	28.389642564824324	27.761576084918776	22.023867533102408
66-67	22.031035059309474	28.39638999263263	27.37940218611102	22.193172761946876
68-69	22.133909928704274	28.055625455594452	27.74802033264661	22.062444283054663
70-71	22.064518528469044	27.54926608124651	27.877385673121335	22.508829717163113
72-73	22.20901316774645	27.636004463152826	27.911082160571993	22.243900208528732
74-75	21.87347255750883	27.956168244372382	27.781514943556758	22.388844254562027
76-77	22.022473315884778	27.933195515730887	27.58054317651288	22.463787991871456
78-79	22.212754902224066	27.87638601828054	27.544027999828423	22.366831079666973
80-81	22.050135409743007	27.696164558393228	27.660733983748376	22.592966048115393
82-83	22.28466990949483	27.564814001316336	27.807195585750595	22.343320503438242
84-85	22.689723754840234	27.458017775597614	27.55157686191867	22.300681607643483
86-87	21.995871450001292	27.728071732611532	27.69785848080586	22.578198336581316
88-89	22.431037514395545	27.58418501365465	27.652973708909883	22.331803763039925
90-91	22.48582445514327	27.51889132986973	27.78144998108004	22.213834233906958
92-93	22.46384733290825	27.575711020213877	27.60609577837021	22.35434586850766
94-95	22.249846380435944	27.573347218949785	27.68031997834451	22.496486422269765
96-97	22.218981735964903	27.736133150335746	27.93889632172543	22.105988791973918
98-99	23.36249439827536	29.655819133335477	27.993451853379902	18.988234615009265
100	22.698490864147512	30.519054183668526	21.63592734521132	25.14652760697264
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2094.0
1	2201.5
2	4475.5
3	7066.5
4	10299.5
5	19136.0
6	26532.5
7	30423.0
8	31820.0
9	29431.0
10	25564.0
11	21980.0
12	23763.0
13	33342.0
14	56601.0
15	90027.5
16	118439.5
17	134997.0
18	137544.0
19	128130.5
20	112313.0
21	94806.5
22	82635.0
23	80294.0
24	87470.0
25	106450.0
26	140778.0
27	178113.0
28	199714.5
29	222173.5
30	243927.0
31	261166.0
32	294109.5
33	335327.0
34	381261.5
35	423914.5
36	473933.5
37	515666.5
38	517548.5
39	519257.0
40	542593.0
41	553906.5
42	551077.0
43	561811.0
44	576506.5
45	584968.5
46	592282.0
47	605022.5
48	626852.0
49	625559.0
50	597512.0
51	570269.0
52	541173.0
53	507610.0
54	470119.5
55	428343.5
56	383773.0
57	338347.5
58	297127.5
59	265854.0
60	226329.0
61	174434.0
62	129080.5
63	92305.5
64	65063.5
65	45723.5
66	32467.0
67	24470.0
68	18066.5
69	12876.0
70	9491.0
71	6711.5
72	4925.0
73	3518.0
74	2415.0
75	1647.5
76	1098.0
77	742.0
78	474.5
79	311.5
80	204.0
81	128.5
82	92.0
83	63.0
84	47.0
85	34.5
86	26.5
87	21.0
88	18.5
89	14.5
90	9.0
91	6.0
92	5.0
93	3.5
94	1.5
95	1.5
96	1.5
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.345478790982359E-5
2	2.5564097028664824E-4
3	6.727393954911795E-6
4	6.727393954911795E-6
5	6.727393954911795E-6
6	6.727393954911795E-6
7	6.727393954911795E-6
8	6.727393954911795E-6
9	6.727393954911795E-6
10-11	0.0
12-13	0.0072857676531694745
14-15	4.5073539497909025E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.6820397247994622E-5
24-25	2.3552547887713294E-5
26-27	0.0
28-29	1.0103173949141093E-5
30-31	6.7437820390762375E-6
32-33	6.757927293690362E-6
34-35	0.0027849408693020133
36-37	0.03149453929768197
38-39	0.13557511594400165
40-41	0.18453005385993454
42-43	0.057634562990281926
44-45	0.007109546223308971
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.012773464502984532
64-65	0.07470395194379878
66-67	0.2019816889332335
68-69	0.21909430639685942
70-71	0.08657752557980297
72-73	0.005911709541094611
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	215.0
20-21	1009.0
22-23	2164.0
24-25	4369.0
26-27	7068.0
28-29	14107.0
30-31	29707.0
32-33	36577.0
34-35	49099.0
36-37	58531.0
38-39	64402.0
40-41	82150.0
42-43	96860.0
44-45	118730.0
46-47	118923.0
48-49	97236.0
50-51	84465.0
52-53	80328.0
54-55	100844.0
56-57	109655.0
58-59	107635.0
60-61	120618.0
62-63	127468.0
64-65	143133.0
66-67	167246.0
68-69	143077.0
70-71	138111.0
72-73	129900.0
74-75	132152.0
76-77	121483.0
78-79	133071.0
80-81	139082.0
82-83	140461.0
84-85	150174.0
86-87	157130.0
88-89	170978.0
90-91	181359.0
92-93	182775.0
94-95	198906.0
96-97	486316.0
98-99	919252.0
100-101	9517831.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.0866226105605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.48217560993473	65.04050035562761
2	8.636437166965608	13.142346708454609
3	2.746643800756736	6.269485509414399
4	1.2722705345951835	3.8721107209711905
5	0.6703921636801674	2.5503937779505
6	0.37091938398076213	1.6933201912731493
7	0.23580034351740053	1.2558876224054283
8	0.16092480507673143	0.9795379930041018
9	0.10239403415059291	0.7011734610590105
>10	0.3177419385124187	3.5425554557629155
>50	0.0025814945107427503	0.1320232424094472
>100	0.0013021771928917617	0.1917363224601338
>500	2.461589843845587E-4	0.11891473642132516
>1k	1.3252411177946705E-4	0.2577150436579644
>5k	1.893201493003927E-5	0.10093394838202438
>10k+	1.893201493003927E-5	0.1513649107461556
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0182181864735383E-5
2	0.0	4.0364363729470766E-5	0.0	0.0	2.690957581964718E-5
3	0.0	4.0364363729470766E-5	0.0	0.0	2.690957581964718E-5
4	0.0	6.054654559420615E-5	0.0	2.0182181864735383E-5	3.363696977455898E-5
5	0.0	6.054654559420615E-5	0.0	2.0182181864735383E-5	3.363696977455898E-5
6	0.0	6.054654559420615E-5	0.0	2.690957581964718E-5	5.381915163929436E-5
7	0.0	6.054654559420615E-5	0.0	2.690957581964718E-5	6.054654559420615E-5
8	0.0	6.054654559420615E-5	0.0	2.690957581964718E-5	8.072872745894153E-5
9	0.0	1.4800266700805949E-4	0.0	4.0364363729470766E-5	9.418351536876512E-5
10-11	0.0	1.4800266700805949E-4	0.0	9.081981839130924E-5	9.418351536876512E-5
12-13	0.0	1.4800266700805949E-4	0.0	1.345478790982359E-4	9.754721234622103E-5
14-15	0.0	2.320950914444569E-4	0.0	1.6482115189533897E-4	1.513663639855154E-4
16-17	0.0	2.3545878842191282E-4	0.0	2.0518551562480976E-4	1.6818484887279485E-4
18-19	0.0	2.556409702866482E-4	0.0	2.3882248539936873E-4	1.8163963678261846E-4
20-21	0.0	2.758231521513836E-4	0.0	2.859142430837513E-4	1.8163963678261846E-4
22-23	0.0	3.027327279710308E-4	0.0	5.079182435958405E-4	2.1191290957972152E-4
24-25	0.0	3.2964230379067794E-4	0.0	0.00118065763908702	2.4554987935428054E-4
26-27	0.0	3.430970917005015E-4	0.0	0.002616956248460688	2.489135763317364E-4
28-29	0.0	3.430970917005015E-4	0.0	0.00719831153175562	2.522772733091923E-4
30-31	0.0	3.834614554299723E-4	0.0	0.00807623644287161	2.556409702866482E-4
32-33	0.0	3.868251524074282E-4	0.0	0.01020882032657865	2.8927794006120716E-4
34-35	0.0	4.440080010241785E-4	0.0	0.01251295275613594	3.3973339472304564E-4
36-37	0.0	5.011908496409287E-4	0.0	0.01615247288574322	3.5991557658778103E-4
38-39	0.0	5.281004254605759E-4	0.0	0.02606528787830575	3.7000666752014873E-4
40-41	0.0	5.684647891900467E-4	0.0	0.03623374384115492	3.868251524074282E-4
42-43	0.0	6.021017589646056E-4	0.0	0.04284340840185577	3.901888493848841E-4
44-45	0.0	7.030126682882826E-4	0.0	0.05068754975328292	4.002799403172518E-4
46-47	0.0	7.669229108599446E-4	0.0	0.05915397504553942	4.2382581915944304E-4
48-49	0.0	7.669229108599446E-4	0.0	0.06834023149097146	4.4400800102417843E-4
50-51	0.0	8.274694564541507E-4	0.0	0.07759376187595264	4.4400800102417843E-4
52-53	0.0	8.341968504090626E-4	0.0	0.10419051387669642	4.675538798663697E-4
54-55	0.0	8.375605473865185E-4	0.0	0.1390081412903424	5.011908496409287E-4
56-57	0.0	8.409242443639743E-4	0.0	0.17668491113482593	5.112819405732964E-4
58-59	0.0	8.51015335296342E-4	0.0	0.20043933918961948	5.112819405732964E-4
60-61	0.0	8.913796990258128E-4	0.0	0.21821984141245135	5.2473672848312E-4
62-63	3.3636969774558973E-6	9.216529718229159E-4	0.0	0.23511905502718977	5.314641224380318E-4
64-65	6.727393954911795E-6	0.0010562008509211519	0.0	0.2416378997694993	5.348278194154877E-4
66-67	6.727393954911795E-6	0.0010662919418535195	0.0	0.24862093469469776	5.381915163929436E-4
68-69	6.727393954911795E-6	0.0011201110934928138	0.0	0.2560210680451007	5.381915163929436E-4
70-71	6.727393954911795E-6	0.001147020669312461	0.0	0.261379437330188	5.718284861675026E-4
72-73	6.727393954911795E-6	0.0011571117602448286	0.0	0.26551342091548125	6.189202438518851E-4
74-75	6.727393954911795E-6	0.0011772939421095642	0.0	0.2689477555294637	6.189202438518851E-4
76-77	6.727393954911795E-6	0.0012176583058390348	0.0	0.27186071711194054	6.189202438518851E-4
78-79	6.727393954911795E-6	0.0012378404877037702	0.0	0.27376456960118056	6.189202438518851E-4
80-81	6.727393954911795E-6	0.0012378404877037702	0.0	0.27451467402715324	6.424661226940764E-4
82-83	6.727393954911795E-6	0.0012412041846812261	0.0	0.27506968402843346	6.761030924686353E-4
84-85	6.727393954911795E-6	0.001251295275613594	0.0	0.275332052392675	6.794667894460913E-4
86-87	6.727393954911795E-6	0.001278204851433241	0.0	0.2755002372415478	6.828304864235472E-4
88	6.727393954911795E-6	0.0012983870332979764	0.0	0.2755069646355027	6.929215773559149E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	24420	0.0	51.197105	1
GTATCAA	45935	0.0	39.209843	1
ATCAACG	56820	0.0	31.112556	3
TCAACGC	57370	0.0	30.836596	4
CAACGCA	58000	0.0	30.457508	5
TATCAAC	58955	0.0	30.369545	2
AACGCAG	59825	0.0	29.623795	6
CTTATAC	15490	0.0	28.951748	1
ACGCAGA	67065	0.0	26.332615	7
CGCAGAG	67940	0.0	25.99365	8
TATACAC	19865	0.0	25.36783	3
GCAGAGT	75920	0.0	23.126528	9
TACATGG	48200	0.0	21.326187	2
GAGTACT	50800	0.0	20.632282	12-13
ACATGGG	49040	0.0	20.299543	3
GTACATG	53440	0.0	19.889814	1
CAGAGTA	75190	0.0	19.846128	10-11
AGAGTAC	71820	0.0	19.56513	10-11
ACACATC	24085	0.0	19.100006	6
TTATACA	25930	0.0	18.809063	2
>>END_MODULE