FastQCFastQC Report
Fri 27 May 2016
SRR2139150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15031378
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA273150.048.5387271
GTATCAA486350.037.7118231
ATCAACG615700.029.4801963
TCAACGC625050.029.0797634
TATCAAC629850.029.0527822
CAACGCA631000.028.7854715
AACGCAG645650.028.2392756
ACGCAGA716850.025.3184197
CGCAGAG728750.024.8938878
CGCTACG4800.024.8043494
GCAGAGT812650.022.2249779
GTGGTAT126050.021.0835861
GAGTACT545450.020.3283412-13
TACATGG507650.020.324412
ACATGGG505750.019.9245153
GTACATG546050.019.50641
TGGTATC130500.019.3286062
CAGAGTA805750.019.31313510-11
AGAGTAC776950.019.0935310-11
GTACTTT596900.018.45735714-15
Produced by FastQC (version 0.11.5)