##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139150_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15031378
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59676305126516	33.0	33.0	33.0	33.0	33.0
2	32.628992564753545	33.0	33.0	33.0	33.0	33.0
3	32.66525258030235	33.0	33.0	33.0	33.0	33.0
4	36.591089253427064	37.0	37.0	37.0	37.0	37.0
5	36.60600584989613	37.0	37.0	37.0	37.0	37.0
6	36.5847668124639	37.0	37.0	37.0	37.0	37.0
7	36.56369269670419	37.0	37.0	37.0	37.0	37.0
8	36.54622763129235	37.0	37.0	37.0	37.0	37.0
9	36.57986533237339	37.0	37.0	37.0	37.0	37.0
10-11	36.55629952889216	37.0	37.0	37.0	37.0	37.0
12-13	36.54813231361756	37.0	37.0	37.0	37.0	37.0
14-15	38.63152486751381	40.0	37.0	40.0	37.0	40.0
16-17	38.57055424326366	40.0	37.0	40.0	37.0	40.0
18-19	38.60466545382599	40.0	37.0	40.0	37.0	40.0
20-21	38.61624984678006	40.0	37.0	40.0	37.0	40.0
22-23	38.60955139500315	40.0	37.0	40.0	37.0	40.0
24-25	38.60904026441092	40.0	37.0	40.0	37.0	40.0
26-27	38.53236808970402	40.0	37.0	40.0	37.0	40.0
28-29	38.42038630052142	40.0	37.0	40.0	37.0	40.0
30-31	38.27723918100109	40.0	37.0	40.0	37.0	40.0
32-33	38.156061606349425	40.0	37.0	40.0	37.0	40.0
34-35	38.11110396612148	40.0	37.0	40.0	37.0	40.0
36-37	38.09504265634324	40.0	37.0	40.0	37.0	40.0
38-39	38.05372169760783	40.0	37.0	40.0	37.0	40.0
40-41	38.031415333321696	40.0	37.0	40.0	37.0	40.0
42-43	37.99317046860311	40.0	37.0	40.0	33.0	40.0
44-45	37.8892493339473	40.0	37.0	40.0	33.0	40.0
46-47	37.81201709970077	40.0	37.0	40.0	33.0	40.0
48-49	37.74271321329357	40.0	37.0	40.0	33.0	40.0
50-51	37.70872950620369	40.0	37.0	40.0	33.0	40.0
52-53	37.65898446427835	40.0	37.0	40.0	33.0	40.0
54-55	37.553556792310246	40.0	37.0	40.0	33.0	40.0
56-57	37.386071060922944	40.0	37.0	40.0	33.0	40.0
58-59	37.16267328238537	37.0	37.0	40.0	33.0	40.0
60-61	37.01344901393436	37.0	37.0	40.0	33.0	40.0
62-63	36.89477430895042	37.0	37.0	40.0	33.0	40.0
64-65	36.72427900770145	37.0	37.0	40.0	33.0	40.0
66-67	36.512096532611764	37.0	37.0	40.0	33.0	40.0
68-69	36.30712384652032	37.0	37.0	40.0	33.0	40.0
70-71	36.091290932618506	37.0	37.0	38.5	33.0	40.0
72-73	35.80276296443421	37.0	35.0	37.0	33.0	40.0
74-75	35.47470902135986	37.0	33.0	37.0	33.0	40.0
76-77	33.19309539092362	35.0	33.0	37.0	27.0	37.0
78-79	34.98118794631509	37.0	33.0	37.0	33.0	37.0
80-81	35.23201623411755	37.0	33.0	37.0	33.0	37.0
82-83	35.091082918079024	37.0	33.0	37.0	33.0	37.0
84-85	34.92409751331966	37.0	33.0	37.0	33.0	37.0
86-87	34.81024862196088	37.0	33.0	37.0	33.0	37.0
88-89	34.70612816052593	37.0	33.0	37.0	33.0	37.0
90-91	34.63749737477272	37.0	33.0	37.0	33.0	37.0
92-93	34.56983694204774	37.0	33.0	37.0	33.0	37.0
94-95	34.5651378131937	37.0	33.0	37.0	33.0	37.0
96-97	34.61033720941577	37.0	33.0	37.0	33.0	37.0
98-99	34.62568945637601	37.0	33.0	37.0	33.0	37.0
100	34.55876537703325	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	4.0
19	11.0
20	96.0
21	444.0
22	1806.0
23	5414.0
24	12967.0
25	26133.0
26	45966.0
27	74218.0
28	109531.0
29	152753.0
30	207079.0
31	276345.0
32	366799.0
33	494084.0
34	686495.0
35	1016610.0
36	1823194.0
37	4495933.0
38	5084267.0
39	151226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.23068032955331	20.686195283372907	14.857580912247762	23.225543474826026
2	14.178304307837134	20.603067261202852	43.19251296875026	22.026115462209752
3	17.775823199697538	26.72438459896256	30.786746949397916	24.71304525194199
4	11.86691013072189	18.004504843435168	39.919642757945596	30.208942267897342
5	12.619621434575059	38.2161768535127	35.964008090276224	13.20019362163602
6	28.864705551280796	38.8038408720744	18.86658694898099	13.46486662766381
7	25.677971773446185	33.35693507275248	23.477368475465124	17.48772467833621
8	23.95922050526572	37.12813954914845	21.154141689471185	17.758498256114642
9	25.224194348648542	17.203133338806328	21.86427618279575	35.70839612974938
10-11	23.96172193926598	27.30063404699157	28.865410742780867	19.872233270961583
12-13	24.86277540808103	24.61773684028788	29.15225105149356	21.36723670013753
14-15	21.87527629836531	25.92567977298046	27.539814407331896	24.659229521322334
16-17	20.899557578819454	29.01550676192163	28.09571417870005	21.989221480558868
18-19	20.72630998967626	27.942215277933936	30.40933772006798	20.922137012321823
20-21	21.930168384317884	27.189273061220216	30.277615658499123	20.602942895962777
22-23	21.367959878613316	27.063352546781278	30.235256477922523	21.333431096682883
24-25	21.023610781140924	27.143657152211976	30.63808803183153	21.19464403481557
26-27	21.03659720888227	27.40591097780458	30.359286198863185	21.198205614449964
28-29	20.846478506819093	27.64510044977002	30.307276399545685	21.201144643865206
30-31	21.31095032342802	27.360298754405036	30.182388094011763	21.14636282815518
32-33	21.078840485830394	27.600578525879865	30.078905122417783	21.241675865871958
34-35	21.133769721354863	27.794969935911208	30.10926384824564	20.961996494488297
36-37	21.33272022950746	27.507255020745337	29.96097243398826	21.19905231575894
38-39	21.1259139658214	27.712595157814242	30.010741915930893	21.150748960433468
40-41	21.494080069524074	27.667707192778124	29.76682701878187	21.071385718915934
42-43	21.479551746701738	27.732581186754977	29.507365135915038	21.280501930628244
44-45	21.49741365133394	28.113696380265925	29.113958371273135	21.274931597127004
46-47	21.63742042662767	27.799656885821488	28.666776668446726	21.896146019104112
48-49	21.698108483652902	27.945662750214133	28.62136081345427	21.73486795267869
50-51	21.453889432814	28.180517050335418	28.449610389377728	21.915983127472856
52-53	21.609708697197032	28.206125476284704	28.297110659822227	21.887055166696033
54-55	21.78616272848083	28.016836116001713	28.286460494062027	21.910540661455432
56-57	21.858915273848588	28.206398094398516	28.037599897426812	21.897086734326084
58-59	21.63335645162657	28.333738745374998	28.379542349571317	21.65336245342711
60-61	21.793136410183646	28.411658562404373	28.012187665403033	21.78301736200895
62-63	21.746475207646224	28.31743622476178	27.965900741672506	21.970187825919485
64-65	21.70000719986189	28.3774854499463	27.97842749463329	21.944079855558524
66-67	21.851617669243176	28.43394091222855	27.712473900079086	22.001967518449188
68-69	21.89537833766428	28.228855511517747	27.92341484775221	21.95235130306576
70-71	21.909108757435924	27.699283136336394	28.014303043471806	22.377305062755873
72-73	22.019556228601214	27.821013271680556	28.053534518085765	22.105895981632468
74-75	21.73182982702257	28.019675903177994	27.97965784681409	22.268836422985355
76-77	21.798483114515303	28.035748616471906	27.857704047911607	22.308064221101183
78-79	21.975043071538014	28.012561452611727	27.771594701313695	22.240800774536567
80-81	21.855044245537616	27.824782011940698	27.90113883203591	22.419034910485777
82-83	22.07386357632245	27.683705091213383	28.015553995470505	22.22687733699366
84-85	22.49965874299606	27.61202384383007	27.74671666350618	22.14160074966769
86-87	21.807632784452295	27.87620336128483	27.903063659500276	22.4131001947626
88-89	22.20847185945022	27.678671807963983	27.927509064285978	22.185347268299818
90-91	22.21186164200922	27.602100666529854	28.021919657872445	22.16411803358848
92-93	22.207203130084817	27.626626076244953	27.92095169095762	22.245219102712614
94-95	22.026773963977185	27.713895092630242	27.936489579209884	22.322841364182686
96-97	21.952801970465792	27.853527684779998	28.182117044961828	22.01155329979238
98-99	23.146883773372654	29.74509444524355	28.31125379757144	18.79676798381236
100	22.40925646848535	30.613412875475056	21.910103593958947	25.06722706208065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2555.0
1	2657.5
2	5098.0
3	7934.5
4	11449.0
5	20814.0
6	28716.5
7	32909.0
8	34341.0
9	31988.5
10	28026.0
11	24260.5
12	26415.5
13	36678.0
14	60802.0
15	94892.0
16	123757.0
17	140687.0
18	143878.5
19	135459.0
20	120139.5
21	103148.5
22	91374.5
23	90255.5
24	99285.5
25	118670.5
26	151279.0
27	189618.5
28	215235.0
29	239509.0
30	264032.5
31	286286.5
32	320445.0
33	357409.0
34	399586.5
35	444154.0
36	493932.5
37	534882.0
38	544050.0
39	548808.5
40	568716.0
41	581350.0
42	585361.0
43	597125.0
44	610840.5
45	623114.0
46	632018.0
47	639358.0
48	638187.5
49	624819.0
50	603766.0
51	573378.5
52	536624.5
53	498291.5
54	458569.5
55	417773.0
56	375974.0
57	330647.5
58	286732.0
59	248424.5
60	205488.5
61	156817.5
62	115520.0
63	83843.5
64	59552.5
65	42191.5
66	30015.5
67	22283.5
68	16292.5
69	11343.5
70	8462.0
71	5856.5
72	4161.0
73	2899.5
74	1923.5
75	1284.5
76	858.5
77	601.5
78	370.5
79	228.0
80	163.5
81	122.0
82	96.5
83	75.0
84	66.5
85	57.0
86	45.5
87	33.5
88	21.5
89	10.5
90	3.5
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.652750000698539E-6
2	3.5259575003702254E-4
3	6.652750000698539E-6
4	6.652750000698539E-6
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.007258150250762106
14-15	4.856507500509933E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.3307169220783736E-5
24-25	4.325657742047253E-5
26-27	0.0
28-29	3.330667467092672E-6
30-31	6.670888004595975E-6
32-33	3.3438481753824295E-6
34-35	0.002710563936517117
36-37	0.031207187828725037
38-39	0.13660250028408005
40-41	0.18702498225239367
42-43	0.05820711151717807
44-45	0.00693426848750709
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	3.733965976699455E-6
62-63	0.012698542809251425
64-65	0.07703200463354976
66-67	0.20827239934639113
68-69	0.22510383937288267
70-71	0.08929365549012962
72-73	0.006197496314188178
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	237.0
20-21	1140.0
22-23	2473.0
24-25	4661.0
26-27	7558.0
28-29	16174.0
30-31	35857.0
32-33	44883.0
34-35	60213.0
36-37	84085.0
38-39	101628.0
40-41	113883.0
42-43	133813.0
44-45	157742.0
46-47	153535.0
48-49	129934.0
50-51	111224.0
52-53	102225.0
54-55	110767.0
56-57	118345.0
58-59	119402.0
60-61	119187.0
62-63	117281.0
64-65	118388.0
66-67	135470.0
68-69	121707.0
70-71	122553.0
72-73	116279.0
74-75	117376.0
76-77	108840.0
78-79	120318.0
80-81	126418.0
82-83	129639.0
84-85	138110.0
86-87	143970.0
88-89	158066.0
90-91	167137.0
92-93	171047.0
94-95	186129.0
96-97	477297.0
98-99	912699.0
100-101	9613688.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.0918976546509
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.28617621626738	66.51965065875658
2	8.309500862951353	12.811903801757579
3	2.582403287987064	5.972471099415982
4	1.1025871209650029	3.4000213393908076
5	0.5856798208884852	2.257558440516469
6	0.35383099669560586	1.636650179058046
7	0.2122207272476755	1.1452349009621365
8	0.14057111189088503	0.8669515016874069
9	0.09822014286335358	0.6814779481122158
>10	0.32245573386425014	3.717900079245679
>50	0.004324950587071217	0.21533427801538652
>100	0.0016942392166987127	0.2501845576671816
>500	1.8600578503104607E-4	0.10150617394747913
>1k	1.1158709273161285E-4	0.20392133103311333
>5k	3.719569735113852E-5	0.21923371043392906
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.652750000698539E-6	0.0
5	0.0	0.0	0.0	6.652750000698539E-6	0.0
6	0.0	0.0	0.0	1.9958250002095615E-5	0.0
7	0.0	0.0	0.0	1.9958250002095615E-5	0.0
8	0.0	0.0	0.0	1.9958250002095615E-5	0.0
9	0.0	6.652750000698539E-6	0.0	3.326375000349269E-5	6.652750000698539E-6
10-11	0.0	6.652750000698539E-6	0.0	3.991650000419123E-5	6.652750000698539E-6
12-13	0.0	6.652750000698539E-6	0.0	4.656925000488977E-5	6.652750000698539E-6
14-15	3.3263750003492694E-6	6.652750000698539E-6	0.0	5.987475000628685E-5	6.652750000698539E-6
16-17	6.652750000698539E-6	6.652750000698539E-6	0.0	7.318025000768392E-5	6.652750000698539E-6
18-19	6.652750000698539E-6	6.652750000698539E-6	0.0	1.3638137501432003E-4	6.652750000698539E-6
20-21	6.652750000698539E-6	6.652750000698539E-6	0.0	1.829506250192098E-4	6.652750000698539E-6
22-23	6.652750000698539E-6	6.652750000698539E-6	0.0	4.1912325004400794E-4	6.652750000698539E-6
24-25	6.652750000698539E-6	6.652750000698539E-6	0.0	0.0010977037501152588	6.652750000698539E-6
26-27	6.652750000698539E-6	6.652750000698539E-6	0.0	0.0030236748753174857	6.652750000698539E-6
28-29	6.652750000698539E-6	6.652750000698539E-6	0.0	0.009217385125967825	6.652750000698539E-6
30-31	6.652750000698539E-6	6.652750000698539E-6	0.0	0.010281825126079593	6.652750000698539E-6
32-33	6.652750000698539E-6	6.652750000698539E-6	0.0	0.012656856876328969	6.652750000698539E-6
34-35	6.652750000698539E-6	6.652750000698539E-6	0.0	0.015610677876639122	6.652750000698539E-6
36-37	6.652750000698539E-6	6.652750000698539E-6	0.0	0.020284234752129843	6.652750000698539E-6
38-39	6.652750000698539E-6	6.652750000698539E-6	0.0	0.03376935900354578	6.652750000698539E-6
40-41	6.652750000698539E-6	6.652750000698539E-6	0.0	0.04879792125512378	6.652750000698539E-6
42-43	6.652750000698539E-6	6.652750000698539E-6	0.0	0.058517589006144344	6.652750000698539E-6
44-45	6.652750000698539E-6	6.652750000698539E-6	0.0	0.0708018918824342	6.652750000698539E-6
46-47	6.652750000698539E-6	6.652750000698539E-6	0.0	0.0837980390087988	6.652750000698539E-6
48-49	6.652750000698539E-6	6.652750000698539E-6	0.0	0.09610895288509144	6.652750000698539E-6
50-51	6.652750000698539E-6	6.652750000698539E-6	0.0	0.10835333926137711	6.652750000698539E-6
52-53	6.652750000698539E-6	6.652750000698539E-6	0.0	0.13610195951429072	6.652750000698539E-6
54-55	6.652750000698539E-6	6.652750000698539E-6	0.0	0.16720023939255602	6.652750000698539E-6
56-57	6.652750000698539E-6	6.652750000698539E-6	0.0	0.1993762647709345	6.652750000698539E-6
58-59	6.652750000698539E-6	6.652750000698539E-6	0.0	0.22943338927409052	6.652750000698539E-6
60-61	6.652750000698539E-6	6.652750000698539E-6	0.0	0.24222330115043345	6.652750000698539E-6
62-63	6.652750000698539E-6	6.652750000698539E-6	0.0	0.25735498102702226	6.652750000698539E-6
64-65	6.652750000698539E-6	6.652750000698539E-6	0.0	0.2632360120276398	6.652750000698539E-6
66-67	6.652750000698539E-6	6.652750000698539E-6	0.0	0.26785634690312493	6.652750000698539E-6
68-69	6.652750000698539E-6	6.652750000698539E-6	0.0	0.27289247865365374	6.652750000698539E-6
70-71	6.652750000698539E-6	6.652750000698539E-6	0.0	0.27719015515410494	6.652750000698539E-6
72-73	6.652750000698539E-6	6.652750000698539E-6	0.0	0.28096891715450173	6.652750000698539E-6
74-75	6.652750000698539E-6	6.652750000698539E-6	0.0	0.28379966227979897	6.652750000698539E-6
76-77	6.652750000698539E-6	6.652750000698539E-6	0.0	0.2864042139050724	6.652750000698539E-6
78-79	6.652750000698539E-6	6.652750000698539E-6	0.0	0.2881705190302579	6.652750000698539E-6
80-81	6.652750000698539E-6	6.652750000698539E-6	0.0	0.28868610715531207	6.652750000698539E-6
82-83	6.652750000698539E-6	6.652750000698539E-6	0.0	0.2891218622803578	6.652750000698539E-6
84-85	6.652750000698539E-6	6.652750000698539E-6	0.0	0.2893879722803857	6.652750000698539E-6
86-87	6.652750000698539E-6	6.652750000698539E-6	0.0	0.28950106903039763	6.652750000698539E-6
88	6.652750000698539E-6	6.652750000698539E-6	0.0	0.2895077217803983	6.652750000698539E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	27315	0.0	48.538727	1
GTATCAA	48635	0.0	37.711823	1
ATCAACG	61570	0.0	29.480196	3
TCAACGC	62505	0.0	29.079763	4
TATCAAC	62985	0.0	29.052782	2
CAACGCA	63100	0.0	28.785471	5
AACGCAG	64565	0.0	28.239275	6
ACGCAGA	71685	0.0	25.318419	7
CGCAGAG	72875	0.0	24.893887	8
CGCTACG	480	0.0	24.80434	94
GCAGAGT	81265	0.0	22.224977	9
GTGGTAT	12605	0.0	21.083586	1
GAGTACT	54545	0.0	20.32834	12-13
TACATGG	50765	0.0	20.32441	2
ACATGGG	50575	0.0	19.924515	3
GTACATG	54605	0.0	19.5064	1
TGGTATC	13050	0.0	19.328606	2
CAGAGTA	80575	0.0	19.313135	10-11
AGAGTAC	77695	0.0	19.09353	10-11
GTACTTT	59690	0.0	18.457357	14-15
>>END_MODULE