FastQCFastQC Report
Fri 27 May 2016
SRR2139149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7191556
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139850.047.6208271
GTATCAA252600.037.72851
ATCAACG301850.031.1677423
TATCAAC320250.029.6139092
ACGCAGA321750.029.3753327
TCAACGC321100.029.3254854
CAACGCA324350.029.045045
AACGCAG325600.028.9871316
CGCAGAG371900.025.3802978
GCAGAGT411800.022.8392589
GTGGTAT68750.022.1328261
TGGTATC67350.021.9670542
ACATGGG241650.020.4603733
TACATGG249300.020.4581072
GAGTACT280550.020.34499412-13
CGCTACG2050.00418185520.2435494
GTACATG271650.019.628321
CAGAGTA410950.019.45506310-11
AGAGTAC395400.018.79682210-11
GTACTTT304000.018.73990614-15
Produced by FastQC (version 0.11.5)