##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139149_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7191556
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69164948447874	33.0	33.0	33.0	33.0	33.0
2	32.76366644436892	33.0	33.0	33.0	33.0	33.0
3	33.47802533971786	33.0	33.0	33.0	33.0	37.0
4	36.602753145494525	37.0	37.0	37.0	37.0	37.0
5	36.61404903194802	37.0	37.0	37.0	37.0	37.0
6	36.58965194736716	37.0	37.0	37.0	37.0	37.0
7	36.57854934314632	37.0	37.0	37.0	37.0	37.0
8	36.55409622062319	37.0	37.0	37.0	37.0	37.0
9	36.590326627505924	37.0	37.0	37.0	37.0	37.0
10-11	36.58271923350107	37.0	37.0	37.0	37.0	37.0
12-13	36.81888961165011	37.0	37.0	37.0	37.0	38.5
14-15	38.642914064772626	40.0	37.0	40.0	37.0	40.0
16-17	38.584465587141366	40.0	37.0	40.0	37.0	40.0
18-19	38.62547173379447	40.0	37.0	40.0	37.0	40.0
20-21	38.635995160702315	40.0	37.0	40.0	37.0	40.0
22-23	38.63282120451284	40.0	37.0	40.0	37.0	40.0
24-25	38.61927349154911	40.0	37.0	40.0	37.0	40.0
26-27	38.53944038241015	40.0	37.0	40.0	37.0	40.0
28-29	38.429763221277234	40.0	37.0	40.0	37.0	40.0
30-31	38.28132544719621	40.0	37.0	40.0	37.0	40.0
32-33	38.16564469118944	40.0	37.0	40.0	37.0	40.0
34-35	38.12921421300847	40.0	37.0	40.0	37.0	40.0
36-37	38.10724466622091	40.0	37.0	40.0	37.0	40.0
38-39	38.06922785367226	40.0	37.0	40.0	37.0	40.0
40-41	38.04631495201694	40.0	37.0	40.0	37.0	40.0
42-43	37.998013850620126	40.0	37.0	40.0	33.0	40.0
44-45	37.89427823832043	40.0	37.0	40.0	33.0	40.0
46-47	37.814697803074694	40.0	37.0	40.0	33.0	40.0
48-49	37.74758301782032	40.0	37.0	40.0	33.0	40.0
50-51	37.711561090550695	40.0	37.0	40.0	33.0	40.0
52-53	37.652623135532394	40.0	37.0	40.0	33.0	40.0
54-55	37.53788897140903	40.0	37.0	40.0	33.0	40.0
56-57	37.36385609754606	40.0	37.0	40.0	33.0	40.0
58-59	37.14070594793607	37.0	37.0	40.0	33.0	40.0
60-61	36.99926260912496	37.0	37.0	40.0	33.0	40.0
62-63	36.87482529001407	37.0	37.0	40.0	33.0	40.0
64-65	36.69345015119248	37.0	37.0	40.0	33.0	40.0
66-67	36.48614263720838	37.0	37.0	40.0	33.0	40.0
68-69	36.28145530424942	37.0	37.0	40.0	33.0	40.0
70-71	36.057179294627204	37.0	37.0	38.5	33.0	40.0
72-73	35.745440169235366	37.0	35.0	37.0	33.0	40.0
74-75	35.13933985822409	37.0	33.0	37.0	30.0	40.0
76-77	33.44878725608541	35.0	33.0	37.0	27.0	37.0
78-79	35.01984176555866	37.0	33.0	37.0	33.0	37.0
80-81	35.2217151445121	37.0	33.0	37.0	33.0	37.0
82-83	35.071704956589116	37.0	33.0	37.0	33.0	37.0
84-85	34.912915141628645	37.0	33.0	37.0	33.0	37.0
86-87	34.79451891460218	37.0	33.0	37.0	33.0	37.0
88-89	34.69687769253247	37.0	33.0	37.0	33.0	37.0
90-91	34.62286465534135	37.0	33.0	37.0	33.0	37.0
92-93	34.55593758754873	37.0	33.0	37.0	33.0	37.0
94-95	34.564084988759454	37.0	33.0	37.0	33.0	37.0
96-97	34.60064808343278	37.0	33.0	37.0	33.0	37.0
98-99	34.599537656196915	37.0	33.0	37.0	33.0	37.0
100	34.5049509359326	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	0.0
18	1.0
19	14.0
20	68.0
21	215.0
22	830.0
23	2300.0
24	5603.0
25	11349.0
26	20374.0
27	33567.0
28	50158.0
29	71168.0
30	96713.0
31	130442.0
32	174556.0
33	236309.0
34	328233.0
35	488007.0
36	880086.0
37	2146405.0
38	2424707.0
39	90450.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.609610058919166	0.02088562851836628	25.223512378697883	29.145991933864586
2	14.667812272649403	23.508190739780765	39.68405358743946	22.139943400130377
3	16.635192249798546	24.917768132205065	33.03780614901784	25.40923346897854
4	12.5584800505593	23.086606443251842	38.20374035935205	26.151173146836808
5	16.98663223739511	36.67130405037575	32.35316979429344	13.988893917935691
6	27.43146649090496	37.69328052138932	20.393656170327557	14.481596817378161
7	25.529120540812027	33.56258367452051	22.60293877986906	18.305357004798406
8	23.670134808099945	32.5014364068082	22.539183453483503	21.28924533160835
9	23.69080049630462	21.105780936546235	23.85992952007154	31.343489047077604
10-11	24.610577236948654	26.406785668572084	28.54149048958358	20.44114660489568
12-13	24.926186638780383	24.459784372649008	28.619281307058298	21.99474768151231
14-15	22.167215367860628	26.606112080770593	27.46761884261592	23.759053708752855
16-17	20.726349068268394	28.961242879844086	28.378594284741716	21.9338137671458
18-19	20.679676275899123	27.802147407320476	30.676664410316768	20.841511906463637
20-21	21.892749063540208	27.139426912681095	30.536595371150955	20.431228652627738
22-23	21.433198015154126	26.857180644658392	30.48683044985153	21.22279089033595
24-25	21.009276881726972	27.106521915272513	30.804025395363748	21.08017580763676
26-27	21.100602675372134	27.34709425865085	30.440206281683597	21.112096784293424
28-29	20.87687800769502	27.581757499358005	30.50425944483778	21.037105048109193
30-31	21.336892543882133	27.331503651378142	30.362530758276023	20.969073046463695
32-33	21.10640651101486	27.637519342722268	30.21147203850754	21.044602107755328
34-35	21.119515806459287	27.587041509732146	30.227036249321838	21.066406434486733
36-37	21.304006461693657	27.468723215891817	30.166938843173423	21.060331479241103
38-39	21.060970044821584	27.838268487881173	30.067557507670138	21.0332039596271
40-41	21.52294547255184	27.673962272148366	29.776124233215366	21.02696802208443
42-43	21.440029441949168	27.642040351881477	29.668914350477017	21.249015855692342
44-45	21.501744456809732	28.24310379627238	29.005231477287985	21.249920269629907
46-47	21.629796429161832	27.973161093452752	28.565313614552274	21.831728862833145
48-49	21.66314660719009	28.021543984568403	28.64469052031039	21.67061888793112
50-51	21.473096524008632	28.303864964524863	28.31857649462462	21.904462016841887
52-53	21.724054530046068	28.14115707346689	28.23342832475332	21.901360071733723
54-55	21.89107757038783	28.01929253140632	28.173065839890388	21.916564058315462
56-57	21.846207801436986	28.35208141378934	27.977114677339713	21.824596107433962
58-59	21.769307855636864	28.33016391070487	28.18336987158197	21.7171583620763
60-61	21.832352959342487	28.239211722863335	28.032801150413896	21.895634167380283
62-63	21.857984588435478	28.19122021122822	27.983221328881225	21.96757387145508
64-65	21.75902147192049	28.255260793530784	27.934918050977863	22.050799683570858
66-67	21.84162534477993	28.34794886531547	27.746045222782143	22.064380567122452
68-69	22.015988290723044	28.211162919757538	27.912037626761926	21.86081116275749
70-71	21.962953787711193	27.623469920712733	28.040907817590856	22.372668473985215
72-73	22.00260152523642	27.883366080103105	28.021647952398876	22.0923844422616
74-75	21.796680943548978	28.00804539963592	28.052411574464315	22.142862082350785
76-77	21.983981099721934	27.949852123011194	27.687706934052965	22.378459843213907
78-79	21.90836178560068	28.005327112713392	27.83101685929382	22.25529424239211
80-81	21.88823005215047	27.945322623728643	27.870001975539292	22.296445348581596
82-83	22.191707241227775	27.629262275699247	28.01630002399302	22.162730459079963
84-85	22.510337706423037	27.558122854515606	27.891382378829437	22.04015706023192
86-87	21.932306478595205	27.85019848859459	27.826593005294654	22.390902027515555
88-89	22.27868242212645	27.652130987305412	27.867901767136168	22.20128482343197
90-91	22.33228228087683	27.668440190837952	27.905928911873083	22.09334861641214
92-93	22.14419969144504	27.761056468287904	27.913941403699138	22.180802436567923
94-95	22.233974664383137	27.57662612589442	27.837640901646864	22.351758308075574
96-97	22.100990412419254	27.920840335505336	28.19611219766286	21.78205705441255
98-99	23.252920877957266	29.967173638810436	27.552948626936256	19.22695685629604
100	22.63522668910428	30.351070339733155	21.808213040770163	25.205489930392407
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1189.0
1	1222.0
2	2470.5
3	3909.0
4	5860.5
5	10946.0
6	14938.5
7	16916.5
8	17707.5
9	16352.0
10	14027.0
11	11958.0
12	12847.0
13	17288.0
14	28936.5
15	47780.0
16	65327.5
17	75743.5
18	76680.0
19	70567.0
20	61725.0
21	52527.0
22	46037.0
23	44740.0
24	49224.0
25	59532.0
26	75825.5
27	93653.0
28	104261.5
29	114953.0
30	126700.0
31	136310.0
32	151566.0
33	169095.0
34	188251.0
35	205463.5
36	226174.0
37	245356.0
38	248829.0
39	252652.5
40	264907.5
41	274023.0
42	277832.5
43	283873.0
44	290406.0
45	295957.5
46	302813.0
47	308219.0
48	307916.0
49	302609.0
50	293331.0
51	279093.0
52	262024.0
53	244648.0
54	226834.5
55	206295.0
56	182392.0
57	158004.5
58	136672.0
59	117592.5
60	96035.5
61	73348.5
62	54170.5
63	38609.5
64	27230.5
65	19548.5
66	14174.0
67	10669.0
68	7868.5
69	5678.0
70	4345.0
71	3194.0
72	2369.5
73	1680.0
74	1169.0
75	807.0
76	550.5
77	349.0
78	212.0
79	124.0
80	81.0
81	49.5
82	33.5
83	26.0
84	21.5
85	16.5
86	12.5
87	13.5
88	10.0
89	4.0
90	1.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.1124157275560393E-4
2	2.7810393188900984E-4
3	1.3905196594450492E-5
4	1.3905196594450492E-5
5	1.3905196594450492E-5
6	1.3905196594450492E-5
7	0.0
8	0.0
9	6.952598297225246E-5
10-11	6.465916416419479E-4
12-13	0.006201717681124919
14-15	3.893455046446138E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.0860611480150782E-5
24-25	5.563969900870225E-5
26-27	0.0
28-29	0.0
30-31	4.882125270033836E-5
32-33	2.167834744978368E-4
34-35	0.005319075404349726
36-37	0.042779830117465785
38-39	0.14318764060093817
40-41	0.1661971055785144
42-43	0.04885615764157439
44-45	0.005271392241675569
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	1.5381986248196653E-5
58-59	1.3182754227864373E-4
60-61	0.001454180075809691
62-63	0.017436634937561552
64-65	0.09182165047072305
66-67	0.2025488445660395
68-69	0.1999358378592582
70-71	0.07302260953963709
72-73	0.004275980357235871
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	125.0
20-21	580.0
22-23	1284.0
24-25	2359.0
26-27	4595.0
28-29	9382.0
30-31	17814.0
32-33	22811.0
34-35	31867.0
36-37	42643.0
38-39	50172.0
40-41	54590.0
42-43	66776.0
44-45	76948.0
46-47	74958.0
48-49	62892.0
50-51	54123.0
52-53	50297.0
54-55	52430.0
56-57	53953.0
58-59	52051.0
60-61	52981.0
62-63	51320.0
64-65	54680.0
66-67	60645.0
68-69	57717.0
70-71	60598.0
72-73	56408.0
74-75	54094.0
76-77	51882.0
78-79	57406.0
80-81	58173.0
82-83	57589.0
84-85	64815.0
86-87	70852.0
88-89	77421.0
90-91	80505.0
92-93	82804.0
94-95	102266.0
96-97	335009.0
98-99	1261221.0
100-101	3660520.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.79889646244965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.24151570087582	67.09494750777165
2	8.501695328273296	13.22845029199252
3	2.6217149981444487	6.118996010840741
4	1.1241695834399146	3.498366121131083
5	0.5571114251670742	2.167132704231049
6	0.29902010589848477	1.395806055539217
7	0.18767108494518056	1.022042231465199
8	0.1148221828318122	0.7146431290979698
9	0.08302189024170101	0.581311029872786
>10	0.2612422566404049	3.098542506970333
>50	0.005246401018964587	0.2812413601379192
>100	0.002198227518194214	0.33196243602281267
>500	2.663803368506008E-4	0.14187124150128
>1k	3.044346678266073E-4	0.32468737342548376
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.3905196594450492E-5	0.0
8	0.0	0.0	0.0	1.3905196594450492E-5	0.0
9	0.0	0.0	0.0	1.3905196594450492E-5	0.0
10-11	0.0	0.0	0.0	1.3905196594450492E-5	0.0
12-13	0.0	1.3905196594450492E-5	0.0	1.3905196594450492E-5	1.3905196594450492E-5
14-15	0.0	4.171558978335147E-5	0.0	1.3905196594450492E-5	1.3905196594450492E-5
16-17	0.0	5.5620786377801966E-5	0.0	1.3905196594450492E-5	1.3905196594450492E-5
18-19	0.0	5.5620786377801966E-5	0.0	1.3905196594450492E-5	1.3905196594450492E-5
20-21	0.0	5.5620786377801966E-5	0.0	2.7810393188900983E-5	1.3905196594450492E-5
22-23	0.0	5.5620786377801966E-5	0.0	5.5620786377801966E-5	1.3905196594450492E-5
24-25	0.0	8.343117956670295E-5	0.0	5.5620786377801966E-5	1.3905196594450492E-5
26-27	0.0	9.733637616115345E-5	0.0	1.1819417105282918E-4	1.3905196594450492E-5
28-29	0.0	9.733637616115345E-5	0.0	3.4067731656403703E-4	1.3905196594450492E-5
30-31	0.0	9.733637616115345E-5	0.0	9.525059667198587E-4	1.3905196594450492E-5
32-33	0.0	9.733637616115345E-5	0.0	0.002148352873842601	1.3905196594450492E-5
34-35	0.0	1.1124157275560393E-4	0.0	0.00367097190093493	1.3905196594450492E-5
36-37	0.0	1.3209936764727967E-4	0.0	0.005589889030969098	1.3905196594450492E-5
38-39	0.0	1.7381495743063114E-4	0.0	0.00890627841874554	1.3905196594450492E-5
40-41	0.0	1.946727523223069E-4	0.0	0.01202104245590245	1.3905196594450492E-5
42-43	0.0	2.2248314551120786E-4	0.0	0.015566867587487326	1.3905196594450492E-5
44-45	0.0	2.2248314551120786E-4	0.0	0.01959242200158074	1.3905196594450492E-5
46-47	0.0	2.2248314551120786E-4	0.0	0.023701407595240864	1.3905196594450492E-5
48-49	0.0	2.2248314551120786E-4	0.0	0.02816497570205947	1.3905196594450492E-5
50-51	0.0	2.2248314551120786E-4	0.0	0.03310827309138662	1.3905196594450492E-5
52-53	0.0	2.2248314551120786E-4	0.0	0.03807938087390267	1.3905196594450492E-5
54-55	0.0	2.2248314551120786E-4	0.0	0.0424942807926407	1.3905196594450492E-5
56-57	0.0	2.2248314551120786E-4	0.0	0.046721460557353656	1.3905196594450492E-5
58-59	0.0	2.2248314551120786E-4	0.0	0.05125455464714451	1.3905196594450492E-5
60-61	0.0	2.2248314551120786E-4	0.0	0.05562773897609919	1.3905196594450492E-5
62-63	0.0	2.2248314551120786E-4	0.0	0.06029293243353733	1.3905196594450492E-5
64-65	0.0	2.2248314551120786E-4	0.0	0.06509717785691997	1.3905196594450492E-5
66-67	0.0	2.5029353870010884E-4	0.0	0.07032553177643336	1.3905196594450492E-5
68-69	0.0	2.5029353870010884E-4	0.0	0.07499767783216872	1.3905196594450492E-5
70-71	0.0	2.711513335917846E-4	0.0	0.07919009460539556	1.3905196594450492E-5
72-73	0.0	2.9200912848346034E-4	0.0	0.08247172100168587	1.3905196594450492E-5
74-75	0.0	2.989617267806856E-4	0.0	0.08518323433760372	1.3905196594450492E-5
76-77	0.0	3.0591432507791084E-4	0.0	0.08789474767352157	1.3905196594450492E-5
78-79	0.0	3.0591432507791084E-4	0.0	0.0892296465465888	1.3905196594450492E-5
80-81	0.0	3.0591432507791084E-4	0.0	0.08975804401717792	1.3905196594450492E-5
82-83	0.0	3.0591432507791084E-4	0.0	0.0901960577099031	1.3905196594450492E-5
84-85	0.0	3.0591432507791084E-4	0.0	0.09036292006903651	1.3905196594450492E-5
86-87	0.0	3.0591432507791084E-4	0.0	0.09047416164179212	1.3905196594450492E-5
88	0.0	3.0591432507791084E-4	0.0	0.0904950194366838	1.3905196594450492E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13985	0.0	47.620827	1
GTATCAA	25260	0.0	37.7285	1
ATCAACG	30185	0.0	31.167742	3
TATCAAC	32025	0.0	29.613909	2
ACGCAGA	32175	0.0	29.375332	7
TCAACGC	32110	0.0	29.325485	4
CAACGCA	32435	0.0	29.04504	5
AACGCAG	32560	0.0	28.987131	6
CGCAGAG	37190	0.0	25.380297	8
GCAGAGT	41180	0.0	22.839258	9
GTGGTAT	6875	0.0	22.132826	1
TGGTATC	6735	0.0	21.967054	2
ACATGGG	24165	0.0	20.460373	3
TACATGG	24930	0.0	20.458107	2
GAGTACT	28055	0.0	20.344994	12-13
CGCTACG	205	0.004181855	20.24354	94
GTACATG	27165	0.0	19.62832	1
CAGAGTA	41095	0.0	19.455063	10-11
AGAGTAC	39540	0.0	18.796822	10-11
GTACTTT	30400	0.0	18.739906	14-15
>>END_MODULE