FastQCFastQC Report
Fri 27 May 2016
SRR2139148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9698408
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142950.052.2662471
GTATCAA266600.039.5799981
TCAACGC325500.031.9380344
ATCAACG326350.031.8149643
TATCAAC336200.031.128052
CAACGCA334600.031.0434915
AACGCAG342600.030.3461176
ACGCAGA392750.026.4964797
CGCAGAG395350.026.3115228
TGGTATC68700.024.6308982
GTGGTAT74650.024.2369841
GCAGAGT457600.022.6373759
GTACATG267850.021.366111
GAGTACT312550.021.00696812-13
TACATGG271000.020.4933532
ACATGGG270550.020.0784023
CAGAGTA446500.019.83237810-11
AGAGTAC417750.019.38970410-11
GTACTTT345750.018.88687314-15
CATGGGG195700.018.5347964
Produced by FastQC (version 0.11.5)