##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139148_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9698408
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.638383742981325	33.0	33.0	33.0	33.0	33.0
2	32.670636665316614	33.0	33.0	33.0	33.0	33.0
3	32.707577470446694	33.0	33.0	33.0	33.0	33.0
4	36.63962765847756	37.0	37.0	37.0	37.0	37.0
5	36.651835022820244	37.0	37.0	37.0	37.0	37.0
6	36.628832175342595	37.0	37.0	37.0	37.0	37.0
7	36.60992061789935	37.0	37.0	37.0	37.0	37.0
8	36.59054145793825	37.0	37.0	37.0	37.0	37.0
9	36.62211406243169	37.0	37.0	37.0	37.0	37.0
10-11	36.599168131511895	37.0	37.0	37.0	37.0	37.0
12-13	36.59249342778732	37.0	37.0	37.0	37.0	37.0
14-15	38.72931103744037	40.0	37.0	40.0	37.0	40.0
16-17	38.670238404076215	40.0	37.0	40.0	37.0	40.0
18-19	38.693861353327264	40.0	37.0	40.0	37.0	40.0
20-21	38.70308846664043	40.0	37.0	40.0	37.0	40.0
22-23	38.695346727974865	40.0	37.0	40.0	37.0	40.0
24-25	38.697091843149735	40.0	37.0	40.0	37.0	40.0
26-27	38.62747690090789	40.0	37.0	40.0	37.0	40.0
28-29	38.521794839439906	40.0	37.0	40.0	37.0	40.0
30-31	38.38719946647507	40.0	37.0	40.0	37.0	40.0
32-33	38.27250901024345	40.0	37.0	40.0	37.0	40.0
34-35	38.22742253162719	40.0	37.0	40.0	37.0	40.0
36-37	38.203306532312624	40.0	37.0	40.0	37.0	40.0
38-39	38.15928186581105	40.0	37.0	40.0	37.0	40.0
40-41	38.12259641876791	40.0	37.0	40.0	37.0	40.0
42-43	38.077286302482825	40.0	37.0	40.0	37.0	40.0
44-45	37.976251795445634	40.0	37.0	40.0	33.0	40.0
46-47	37.89100314775855	40.0	37.0	40.0	33.0	40.0
48-49	37.82122212897205	40.0	37.0	40.0	33.0	40.0
50-51	37.78408401127927	40.0	37.0	40.0	33.0	40.0
52-53	37.72847344462646	40.0	37.0	40.0	33.0	40.0
54-55	37.61892458769609	40.0	37.0	40.0	33.0	40.0
56-57	37.45084941253053	40.0	37.0	40.0	33.0	40.0
58-59	37.2302722434942	38.5	37.0	40.0	33.0	40.0
60-61	37.071992899306316	37.0	37.0	40.0	33.0	40.0
62-63	36.94227228915935	37.0	37.0	40.0	33.0	40.0
64-65	36.76742861935176	37.0	37.0	40.0	33.0	40.0
66-67	36.54832159006031	37.0	37.0	40.0	33.0	40.0
68-69	36.34431530442471	37.0	37.0	40.0	33.0	40.0
70-71	36.117904863318486	37.0	37.0	38.5	33.0	40.0
72-73	35.82127753094788	37.0	37.0	37.0	33.0	40.0
74-75	35.48753454918393	37.0	33.0	37.0	33.0	40.0
76-77	33.22392455891577	35.0	33.0	37.0	27.0	37.0
78-79	34.997630702539496	37.0	33.0	37.0	33.0	37.0
80-81	35.240974384489874	37.0	33.0	37.0	33.0	37.0
82-83	35.100544493561735	37.0	33.0	37.0	33.0	37.0
84-85	34.92928206506429	37.0	33.0	37.0	33.0	37.0
86-87	34.81388844159492	37.0	33.0	37.0	33.0	37.0
88-89	34.70693968078366	37.0	33.0	37.0	33.0	37.0
90-91	34.6359259817726	37.0	33.0	37.0	33.0	37.0
92-93	34.559851834521474	37.0	33.0	37.0	33.0	37.0
94-95	34.556149219075664	37.0	33.0	37.0	33.0	37.0
96-97	34.595687734058544	37.0	33.0	37.0	33.0	37.0
98-99	34.606809052207666	37.0	33.0	37.0	33.0	37.0
100	34.5351323935343	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	6.0
20	35.0
21	246.0
22	897.0
23	3008.0
24	7510.0
25	14894.0
26	26949.0
27	44273.0
28	65774.0
29	93441.0
30	128291.0
31	171783.0
32	230707.0
33	310084.0
34	436525.0
35	649368.0
36	1177853.0
37	2908534.0
38	3342045.0
39	86182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.83564972725421	19.940221116702865	14.778373935186066	23.445755220856867
2	14.44362597913381	20.40671172716894	42.65364028257125	22.496022011125998
3	18.537898178752638	25.44255717020773	30.12825403921963	25.89129061182
4	12.414377699927657	17.685789255308705	38.82710440723879	31.07272863752484
5	13.43417393865055	37.29418271534875	35.46075809555548	13.81088525044523
6	30.13814226004928	37.634269459482425	18.559891479096365	13.667696801371937
7	26.730634553629834	32.58464688225119	22.701014434534	17.98370412958498
8	24.090438348232	37.19705337205859	20.806229228549675	17.90627905115974
9	25.18626768434572	17.411208107557446	21.513159685589635	35.8893645225072
10-11	24.198801494018397	27.000281901936894	28.500120844575726	20.300795759468976
12-13	24.969035137301574	24.39066517469555	29.03536106630804	21.604938621694835
14-15	21.939439545362237	25.846121279183482	27.028161518261058	25.186277657193223
16-17	21.28600384722936	29.006353413879886	27.730644039722808	21.976998699167947
18-19	21.180641193894918	27.8527877977499	29.969279494119032	20.997291514236153
20-21	21.92585004307507	27.050236719551137	30.08497466278484	20.938938574588956
22-23	21.621812811338064	26.923924488657182	29.997214733718337	21.457047966286417
24-25	21.38170725846093	27.064744102653908	30.075799815219	21.47774882366616
26-27	21.33942084549915	27.280961270623212	29.97632376229794	21.4032941215797
28-29	21.292383029115854	27.404306308999516	29.944660923379807	21.358649738504823
30-31	21.62268194346389	27.180101584446202	29.856406979301177	21.34080949278873
32-33	21.3407183861934	27.380457907786916	29.778277591713557	21.500546114306125
34-35	21.489443257424345	27.445460574232257	29.744157766783015	21.320938401560387
36-37	21.522886095896144	27.363974169282233	29.65514484283065	21.45799489199097
38-39	21.387921569086892	27.54337928297801	29.64492423018467	21.42377491775043
40-41	21.70835297685623	27.442350519206492	29.44626575294046	21.40303075099682
42-43	21.610518004591956	27.568291883302443	29.164827490897434	21.65636262120817
44-45	21.72328026597698	27.757670512638	28.876007448145657	21.64304177323936
46-47	21.805681765881364	27.760356293087668	28.53871990084417	21.895242040186794
48-49	21.750562920774684	27.898453380396898	28.43426711612228	21.916716582706137
50-51	21.77158550882289	28.064121916346192	28.281230377883233	21.883062196947684
52-53	21.928481385192164	27.99557979639904	28.095578006135803	21.980360812273
54-55	21.96287002664144	27.88524581071271	28.123713419091388	22.02817074355446
56-57	21.959853315310276	28.039541877950885	28.05050020278977	21.95010460394907
58-59	21.90898225950824	28.037077844471074	28.08112209207676	21.972817803943926
60-61	22.006339640362377	27.959317845752192	28.113286130778842	21.921056383106592
62-63	21.95791623472512	28.056276230843054	28.009054180488935	21.976753353942897
64-65	21.957332191921296	28.043842968310113	27.98465557127081	22.014169268497785
66-67	22.027401249890968	28.025186967192145	27.83525294616221	22.112158836754677
68-69	21.993511070220805	28.032085895064103	27.876706313579223	22.09769672113587
70-71	22.01230203830021	27.80783671997758	27.936330097544303	22.243531144177908
72-73	22.086214215958318	27.837441059951278	27.880283785743277	22.196060938347124
74-75	21.944236853987924	27.983272441219558	27.83172390900961	22.24076679578291
76-77	22.123838797987915	27.923903605824517	27.72706118698409	22.22519640920347
78-79	22.059753983628703	27.9222920531218	27.685178178039198	22.332775785210295
80-81	22.09157763173955	27.89717512365509	27.734827773524547	22.276419471080818
82-83	22.142153497734636	27.88877458294379	27.76936060565809	22.19971131366348
84-85	22.250162543425198	27.870351118878045	27.6836242170955	22.195862120601255
86-87	22.081151537229655	27.892538682175072	27.765899496329144	22.260410284266133
88-89	22.20653782969249	27.871171774092257	27.673260985167314	22.249029411047935
90-91	22.200049573800037	27.885389017398328	27.70799728868377	22.20656412011786
92-93	22.20675348448567	27.885385258582623	27.69484924343865	22.213012013493056
94-95	22.10590439574356	27.929197907500075	27.727474939683137	22.237422757073226
96-97	22.19562609914153	28.02275129911705	27.788925120233472	21.992697481507953
98-99	23.308861587076738	29.942327351244714	27.963298832496264	18.785512229182288
100	22.293564172493607	30.904079778812555	21.75495583874801	25.047400209945824
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2905.0
1	2517.0
2	3161.0
3	4537.0
4	6053.5
5	9838.0
6	13353.5
7	15970.5
8	18110.5
9	18284.5
10	16657.0
11	14731.5
12	15761.5
13	21088.0
14	33506.5
15	50869.5
16	65663.0
17	74997.0
18	77475.0
19	73441.0
20	65629.0
21	57021.5
22	50906.0
23	50027.5
24	55183.0
25	66161.0
26	81888.0
27	99082.0
28	116037.5
29	135516.0
30	156052.5
31	175605.5
32	196907.5
33	219219.0
34	240965.0
35	261662.5
36	284702.0
37	307021.0
38	323436.0
39	336468.0
40	352274.5
41	367193.5
42	377169.0
43	387132.0
44	394335.0
45	400994.5
46	404880.0
47	404470.0
48	403763.0
49	397310.0
50	384998.5
51	367075.0
52	343876.0
53	319449.5
54	293285.0
55	265686.0
56	238737.5
57	210490.0
58	183082.5
59	157136.0
60	129566.0
61	104123.0
62	81632.5
63	61738.5
64	45705.5
65	33717.0
66	25188.5
67	19304.5
68	14703.5
69	10670.5
70	7610.0
71	5521.5
72	4050.0
73	2857.5
74	1991.0
75	1410.0
76	961.0
77	599.0
78	368.5
79	256.5
80	193.0
81	129.0
82	82.5
83	57.5
84	50.0
85	46.0
86	37.5
87	29.0
88	19.0
89	9.5
90	5.0
91	2.5
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.124388250112802E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0074496762767662485
14-15	5.103930459514593E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.5779947830666372E-5
24-25	5.1568683246273366E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.002708207175151068
36-37	0.03196168677359626
38-39	0.13733526649408614
40-41	0.1878302135302586
42-43	0.05823748468912638
44-45	0.0072900151747001965
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.012732540952734572
64-65	0.07456703078125676
66-67	0.20056646451386498
68-69	0.2166886604969316
70-71	0.08639121454205319
72-73	0.005823057093327882
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	116.0
20-21	571.0
22-23	1275.0
24-25	2843.0
26-27	4554.0
28-29	7972.0
30-31	17099.0
32-33	20518.0
34-35	27078.0
36-37	34271.0
38-39	38832.0
40-41	48257.0
42-43	59834.0
44-45	73505.0
46-47	75463.0
48-49	60389.0
50-51	52054.0
52-53	55044.0
54-55	53662.0
56-57	52207.0
58-59	55295.0
60-61	50875.0
62-63	35917.0
64-65	57233.0
66-67	72752.0
68-69	67523.0
70-71	69921.0
72-73	67335.0
74-75	66878.0
76-77	61070.0
78-79	69479.0
80-81	73937.0
82-83	70641.0
84-85	81390.0
86-87	88106.0
88-89	94553.0
90-91	99248.0
92-93	102854.0
94-95	121960.0
96-97	331405.0
98-99	624205.0
100-101	6650287.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.35112385827262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.41256876383845	73.69286969662743
2	8.003829251741628	13.342563266047636
3	1.969975239844085	4.925989506419239
4	0.759921242523963	2.5336115843258904
5	0.34386422455675675	1.4330734785729549
6	0.17447645001637108	0.8725684917399754
7	0.10517143881262625	0.6136310335976766
8	0.0596182870328233	0.39754009813527225
9	0.03955931842641841	0.2967582284918322
>10	0.1281174456529186	1.615819271142119
>50	0.002212952779623073	0.12112369092051584
>100	5.806718859750768E-4	0.07267130799134226
>500	6.282832912186174E-5	0.031577967352632054
>1k	4.1884559185198445E-5	0.050202378635457574
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.0310970625282005E-5	0.0
3	0.0	0.0	0.0	1.0310970625282005E-5	0.0
4	0.0	4.124388250112802E-5	0.0	3.093291187584601E-5	0.0
5	0.0	4.124388250112802E-5	0.0	4.124388250112802E-5	0.0
6	0.0	4.124388250112802E-5	0.0	4.124388250112802E-5	0.0
7	0.0	4.124388250112802E-5	0.0	5.1554853126410026E-5	0.0
8	0.0	6.186582375169203E-5	0.0	6.186582375169203E-5	0.0
9	0.0	1.4435358875394808E-4	0.0	1.0310970625282005E-4	0.0
10-11	0.0	1.4435358875394808E-4	0.0	1.3919810344130708E-4	0.0
12-13	0.0	1.4435358875394808E-4	0.0	1.4950907406658908E-4	5.1554853126410025E-6
14-15	0.0	1.907529565677171E-4	0.0	1.959084418803581E-4	2.5777426563205013E-5
16-17	0.0	1.959084418803581E-4	0.0	1.959084418803581E-4	3.093291187584601E-5
18-19	0.0	2.216858684435631E-4	0.0	2.216858684435631E-4	4.124388250112802E-5
20-21	0.0	2.577742656320501E-4	0.0	2.371523243814861E-4	4.6399367813769026E-5
22-23	0.0	3.2995106000902416E-4	0.0	2.6292975094469116E-4	8.248776500225604E-5
24-25	0.0	3.4026203063430616E-4	0.0	2.7324072156997316E-4	1.1342067687810205E-4
26-27	0.0	3.6088397188487016E-4	0.0	3.0417363344581916E-4	1.1342067687810205E-4
28-29	0.0	3.6088397188487016E-4	0.0	4.279052809492032E-4	1.1857616219074305E-4
30-31	0.0	4.175943103239212E-4	0.0	7.475453703329454E-4	1.2373164750338405E-4
32-33	0.0	4.227497956365622E-4	0.0	0.0015311791378543778	1.2373164750338405E-4
34-35	0.0	4.897711047008953E-4	0.0	0.0022941909641252464	1.3404261812866608E-4
36-37	0.0	5.567924137652283E-4	0.0	0.0034335532182189076	1.3404261812866608E-4
38-39	0.0	5.774143550157923E-4	0.0	0.005119396915452516	1.3404261812866608E-4
40-41	0.0	6.495911493927663E-4	0.0	0.006784618671435559	1.4435358875394808E-4
42-43	0.0	6.702130906433303E-4	0.0	0.00854263916304614	1.4950907406658908E-4
44-45	0.0	7.114569731444583E-4	0.0	0.010795586244670259	1.5466455937923008E-4
46-47	0.0	7.527008556455864E-4	0.0	0.013589859284121684	1.5982004469187108E-4
48-49	0.0	7.733227968961504E-4	0.0	0.016533641397639694	1.6497553000451208E-4
50-51	0.0	8.197221647099195E-4	0.0	0.019477423511157707	1.6497553000451208E-4
52-53	0.0	8.300331353352014E-4	0.0	0.022735690228746823	1.7013101531715308E-4
54-55	0.0	8.454995912731244E-4	0.0	0.025932091122584242	1.7528650062979408E-4
56-57	0.0	8.867434737742524E-4	0.0	0.028814007412350563	1.7528650062979408E-4
58-59	0.0	8.970544443995344E-4	0.0	0.031742323069930654	1.8044198594243508E-4
60-61	0.0	9.073654150248164E-4	0.0	0.034582995477195844	1.8559747125507608E-4
62-63	0.0	9.228318709627395E-4	0.0	0.037047317456638244	1.959084418803581E-4
64-65	0.0	0.0010826519156546105	0.0	0.039578660745144975	1.959084418803581E-4
66-67	0.0	0.0010878074009672517	0.0	0.043218433375869524	1.959084418803581E-4
68-69	0.0	0.0011290512834683797	0.0	0.04716753512535253	1.959084418803581E-4
70-71	0.0	0.0011599841953442256	0.0	0.05086917357982877	2.062194125056401E-4
72-73	0.0	0.0011702951659695077	0.0	0.05404495253241563	2.062194125056401E-4
74-75	0.0	0.0011909171072200715	0.0	0.05642678674685577	2.062194125056401E-4
76-77	0.0	0.0012166945337832766	0.0	0.05860755703410292	2.062194125056401E-4
78-79	0.0	0.0012270055044085585	0.0	0.06010264777476881	2.062194125056401E-4
80-81	0.0	0.0012321609897211995	0.0	0.06063366276197083	2.165303831309221E-4
82-83	0.0	0.0012476274456591225	0.0	0.06101001318979363	2.319968390688451E-4
84-85	0.0	0.0012476274456591225	0.0	0.06120076614636134	2.371523243814861E-4
86-87	0.0	0.0012579384162844046	0.0	0.06133996424980265	2.371523243814861E-4
88	0.0	0.0013610481225372246	0.0	0.06139151910292906	2.371523243814861E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14295	0.0	52.266247	1
GTATCAA	26660	0.0	39.579998	1
TCAACGC	32550	0.0	31.938034	4
ATCAACG	32635	0.0	31.814964	3
TATCAAC	33620	0.0	31.12805	2
CAACGCA	33460	0.0	31.043491	5
AACGCAG	34260	0.0	30.346117	6
ACGCAGA	39275	0.0	26.496479	7
CGCAGAG	39535	0.0	26.311522	8
TGGTATC	6870	0.0	24.630898	2
GTGGTAT	7465	0.0	24.236984	1
GCAGAGT	45760	0.0	22.637375	9
GTACATG	26785	0.0	21.36611	1
GAGTACT	31255	0.0	21.006968	12-13
TACATGG	27100	0.0	20.493353	2
ACATGGG	27055	0.0	20.078402	3
CAGAGTA	44650	0.0	19.832378	10-11
AGAGTAC	41775	0.0	19.389704	10-11
GTACTTT	34575	0.0	18.886873	14-15
CATGGGG	19570	0.0	18.534796	4
>>END_MODULE