FastQCFastQC Report
Fri 27 May 2016
SRR2139147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31623305
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA484750.050.93381
GTATCAA884800.037.845541
ATCAACG1076400.030.5795963
TCAACGC1080000.030.4736964
CAACGCA1102800.029.8320085
TATCAAC1117800.029.7191892
AACGCAG1125700.029.2155696
ACGCAGA1272350.025.8369437
CGCAGAG1297600.025.3343018
GTGGTAT236900.024.1791821
TGGTATC226950.023.5213992
GCAGAGT1486100.022.0602979
GAGTACT1004700.020.63700512-13
GTACATG911100.020.5631071
TACATGG891500.020.3616242
ACATGGG888000.019.6457863
CAGAGTA1468950.019.44899710-11
AGAGTAC1375250.018.99503710-11
GTACTTT1105500.018.75943614-15
AGTACTT1080200.017.82411812-13
Produced by FastQC (version 0.11.5)