##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139147_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31623305
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60026338170536	33.0	33.0	33.0	33.0	33.0
2	32.63306972500186	33.0	33.0	33.0	33.0	33.0
3	32.67131740341498	33.0	33.0	33.0	33.0	33.0
4	36.59556184908567	37.0	37.0	37.0	37.0	37.0
5	36.608525073517775	37.0	37.0	37.0	37.0	37.0
6	36.586303171031616	37.0	37.0	37.0	37.0	37.0
7	36.5673979048047	37.0	37.0	37.0	37.0	37.0
8	36.54771593291719	37.0	37.0	37.0	37.0	37.0
9	36.581956819503844	37.0	37.0	37.0	37.0	37.0
10-11	36.55700931638866	37.0	37.0	37.0	37.0	37.0
12-13	36.5508094109708	37.0	37.0	37.0	37.0	37.0
14-15	38.64923554005503	40.0	37.0	40.0	37.0	40.0
16-17	38.58860272511048	40.0	37.0	40.0	37.0	40.0
18-19	38.61287335400269	40.0	37.0	40.0	37.0	40.0
20-21	38.62162899353004	40.0	37.0	40.0	37.0	40.0
22-23	38.61448538430301	40.0	37.0	40.0	37.0	40.0
24-25	38.61617471787198	40.0	37.0	40.0	37.0	40.0
26-27	38.54129375947903	40.0	37.0	40.0	37.0	40.0
28-29	38.43191355775103	40.0	37.0	40.0	37.0	40.0
30-31	38.29546256096967	40.0	37.0	40.0	37.0	40.0
32-33	38.18159347829511	40.0	37.0	40.0	37.0	40.0
34-35	38.14021565194569	40.0	37.0	40.0	37.0	40.0
36-37	38.119551774758854	40.0	37.0	40.0	37.0	40.0
38-39	38.08359754768088	40.0	37.0	40.0	37.0	40.0
40-41	38.0534411083551	40.0	37.0	40.0	37.0	40.0
42-43	38.012660166188496	40.0	37.0	40.0	33.0	40.0
44-45	37.915079473203036	40.0	37.0	40.0	33.0	40.0
46-47	37.832757640750515	40.0	37.0	40.0	33.0	40.0
48-49	37.76710573300657	40.0	37.0	40.0	33.0	40.0
50-51	37.736313670468576	40.0	37.0	40.0	33.0	40.0
52-53	37.68670456161696	40.0	37.0	40.0	33.0	40.0
54-55	37.58072718828963	40.0	37.0	40.0	33.0	40.0
56-57	37.419925433576886	40.0	37.0	40.0	33.0	40.0
58-59	37.20355037573232	38.5	37.0	40.0	33.0	40.0
60-61	37.051879551922404	37.0	37.0	40.0	33.0	40.0
62-63	36.93192882645205	37.0	37.0	40.0	33.0	40.0
64-65	36.7648456557382	37.0	37.0	40.0	33.0	40.0
66-67	36.55245575420528	37.0	37.0	40.0	33.0	40.0
68-69	36.35159486851763	37.0	37.0	40.0	33.0	40.0
70-71	36.123302084808586	37.0	37.0	38.5	33.0	40.0
72-73	35.82596247227123	37.0	37.0	37.0	33.0	40.0
74-75	35.489781705838595	37.0	33.0	37.0	33.0	40.0
76-77	33.22781602445342	35.0	33.0	37.0	27.0	37.0
78-79	34.99751981009676	37.0	33.0	37.0	33.0	37.0
80-81	35.235720265542184	37.0	33.0	37.0	33.0	37.0
82-83	35.09008723872877	37.0	33.0	37.0	33.0	37.0
84-85	34.919815097328524	37.0	33.0	37.0	33.0	37.0
86-87	34.80255285175693	37.0	33.0	37.0	33.0	37.0
88-89	34.6929341879207	37.0	33.0	37.0	33.0	37.0
90-91	34.619725178396855	37.0	33.0	37.0	33.0	37.0
92-93	34.54398507253417	37.0	33.0	37.0	33.0	37.0
94-95	34.54138887301251	37.0	33.0	37.0	33.0	37.0
96-97	34.585507082733116	37.0	33.0	37.0	33.0	37.0
98-99	34.5982542864451	37.0	33.0	37.0	33.0	37.0
100	34.52771800726475	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	28.0
20	193.0
21	944.0
22	3800.0
23	11261.0
24	26537.0
25	53932.0
26	95896.0
27	154237.0
28	230141.0
29	323477.0
30	441419.0
31	589001.0
32	781546.0
33	1049086.0
34	1455613.0
35	2148477.0
36	3816786.0
37	9266194.0
38	1.088621E7
39	288524.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.485161022859565	20.221551795424293	14.845203561107862	23.44808362060828
2	14.325538588961997	20.552706450722173	42.8393968375358	22.28235812278003
3	18.4231249706506	25.65129419584702	30.22687856313564	25.69870227036674
4	12.27749597962642	17.905054515965364	39.162608082868	30.654841421540226
5	13.298926219128582	37.481680045776365	35.56642798720754	13.652965747887515
6	29.761323176056393	37.8446307240815	18.851081504605542	13.542964595256567
7	26.50615740511626	32.68486959222004	22.929184663019882	17.879788339643817
8	23.89731244093557	37.33438045137913	20.99468414196429	17.77362296572101
9	25.13916239937603	17.49015797052206	21.68696156205052	35.6837180680514
10-11	24.036820313373315	27.12143148858097	28.687079038702628	20.154669159343086
12-13	24.836494248716022	24.526456443913467	29.18106355487215	21.45598575249836
14-15	21.8177938400605	25.974270780696855	27.216700713716023	24.991234665526616
16-17	21.136214257175208	29.085435567218543	27.91777298419631	21.860577191409945
18-19	21.045358794724333	28.056219930206538	30.068330935049325	20.830090340019805
20-21	21.820772159854968	27.14208526598454	30.229751324094558	20.807391250065933
22-23	21.495504902645006	27.127240051353567	30.023870783627967	21.353384262373464
24-25	21.27617177757776	27.236647176362656	30.135918533052397	21.351262513007192
26-27	21.24221319710219	27.453255450801585	30.002996536737637	21.301534815358593
28-29	21.179135828620534	27.626445216928396	29.938238238987765	21.2561807154633
30-31	21.465291203098367	27.405488362540865	29.892735022287116	21.23648541207366
32-33	21.23290801968608	27.53655643172977	29.830930384442915	21.399605164141235
34-35	21.39300663628755	27.64853917176051	29.7826371459667	21.175817045985244
36-37	21.39921061123162	27.582807129317516	29.693107524059865	21.324874735391
38-39	21.28690387605852	27.752842056275256	29.680378271603015	21.2798757960632
40-41	21.599986849738176	27.637681783745567	29.459882146446624	21.30244922006963
42-43	21.473627211477524	27.757262184173552	29.2254360593057	21.54367454504322
44-45	21.595792422887573	27.8926649583411	29.006432378079634	21.505110240691693
46-47	21.636134063659394	27.936838587274448	28.65570247832864	21.77132487073752
48-49	21.664654832268692	28.019920293891314	28.52082035116229	21.7946045226777
50-51	21.681299317485767	28.16451005812266	28.35968205213722	21.79450857225435
52-53	21.814943551838294	28.13742457849242	28.212344789451265	21.835287080218023
54-55	21.810428427764183	28.040376686120243	28.26612522030676	21.883069665808815
56-57	21.788453008469606	28.14432155148768	28.20905276446195	21.85817267558077
58-59	21.814140482913807	28.205512603119782	28.196674692990932	21.783672220975483
60-61	21.923256965914874	28.141656480263283	28.163028376179998	21.772058177641846
62-63	21.895522987416914	28.18078134205104	28.105628248360375	21.81806742217167
64-65	21.84956486435759	28.149910318288608	28.05553893830939	21.944985879044424
66-67	21.885521138355056	28.16626497938622	27.950768514935202	21.997445367323518
68-69	21.88641048207735	28.119792158039914	28.012131725124327	21.981665634758407
70-71	21.89356121050321	27.94548849622453	27.99969768893769	22.16125260433457
72-73	21.955097851563533	27.978523927690564	27.96741802569353	22.09896019505237
74-75	21.857510364358404	28.104171205314515	27.90144637032812	22.136872059998964
76-77	21.953233890467946	28.00898283265157	27.847378561860996	22.190404715019486
78-79	21.919642996989566	27.978056204354218	27.84216097702904	22.260139821627174
80-81	21.950894152835815	28.018505432578657	27.856071329794375	22.174529084791157
82-83	21.998179159125733	28.01499609458406	27.929895850674324	22.056928895615886
84-85	22.135258790245725	27.99644989140714	27.80037427725696	22.067917041090173
86-87	21.957560963577507	28.04626364736331	27.869503495359282	22.126671893699896
88-89	22.05129823927956	27.986620098648174	27.812046187091266	22.150035474981
90-91	22.064126836014555	28.010050804446802	27.810126387575146	22.1156959719635
92-93	22.094358660134763	27.991192316959363	27.84727708990815	22.067171932997724
94-95	21.978690998572123	28.072789842196343	27.859926117327532	22.088593041904005
96-97	22.042005052777473	28.158473893181966	27.914365682909686	21.885155371130875
98-99	23.137068164062097	30.015753341851187	28.149463814850357	18.69771467923636
100	22.08181017761885	31.032886282169294	21.875749475328647	25.00955406488321
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9946.0
1	8606.0
2	10814.5
3	15343.0
4	20619.5
5	33546.0
6	44733.5
7	52927.0
8	59292.5
9	59177.5
10	54277.5
11	48900.5
12	52544.0
13	69825.5
14	110537.0
15	166814.0
16	213845.5
17	242609.5
18	249412.0
19	237228.5
20	214638.0
21	189400.0
22	172797.5
23	174347.5
24	195028.5
25	233577.5
26	286114.0
27	344177.0
28	403972.0
29	471398.0
30	537880.0
31	603021.0
32	673925.0
33	744825.5
34	817980.5
35	886594.0
36	959338.0
37	1034169.5
38	1094418.5
39	1143791.5
40	1192211.0
41	1235145.5
42	1266946.5
43	1296407.0
44	1320172.5
45	1344769.5
46	1358586.0
47	1353773.5
48	1341875.5
49	1315103.0
50	1275908.5
51	1213007.0
52	1128493.5
53	1040791.0
54	957601.5
55	868794.5
56	774273.5
57	679180.0
58	585187.0
59	496812.0
60	404491.0
61	320874.5
62	250147.5
63	189788.0
64	141362.5
65	105128.0
66	80458.5
67	60940.5
68	44907.0
69	33117.0
70	23694.5
71	16487.0
72	11787.5
73	8378.5
74	6066.5
75	4238.0
76	2817.0
77	1898.5
78	1330.0
79	968.0
80	710.0
81	493.0
82	359.0
83	251.0
84	157.5
85	102.0
86	76.0
87	59.5
88	45.0
89	35.0
90	23.0
91	15.5
92	12.5
93	7.0
94	2.5
95	2.0
96	2.0
97	2.0
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.6998030408270103E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.007344267147282677
14-15	4.854015100572189E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.487780505637662E-6
24-25	4.5866092267141326E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0028027170940868626
36-37	0.031220559393717578
38-39	0.1364219412075952
40-41	0.18674063270811872
42-43	0.0580931444052302
44-45	0.00708196704095858
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	1.7456420439143138E-6
62-63	0.012300615145075237
64-65	0.0758937123768087
66-67	0.2013016705527564
68-69	0.21783786954513157
70-71	0.08775957942216518
72-73	0.005798822696218624
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	568.0
20-21	2112.0
22-23	4720.0
24-25	9423.0
26-27	15633.0
28-29	28722.0
30-31	61998.0
32-33	78213.0
34-35	106359.0
36-37	152244.0
38-39	184201.0
40-41	198190.0
42-43	239225.0
44-45	282647.0
46-47	276588.0
48-49	239433.0
50-51	208606.0
52-53	197015.0
54-55	215594.0
56-57	215784.0
58-59	209835.0
60-61	215397.0
62-63	206454.0
64-65	214562.0
66-67	240654.0
68-69	223732.0
70-71	225160.0
72-73	223785.0
74-75	225277.0
76-77	209662.0
78-79	225361.0
80-81	234302.0
82-83	259599.0
84-85	263025.0
86-87	280048.0
88-89	306312.0
90-91	322697.0
92-93	333070.0
94-95	397352.0
96-97	1059674.0
98-99	1994523.0
100-101	2.1035549E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.38806367210165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.06807163186141	66.60641407581495
2	8.608243793526976	13.323506375968785
3	2.565952003933328	5.957221711798477
4	1.100247733403475	3.4058416659085453
5	0.5741529381310534	2.2216292066805097
6	0.31668136128574054	1.4704414410569229
7	0.20462196327053353	1.1084708265603347
8	0.13247984193413334	0.8201886754294939
9	0.09558650956931165	0.665752939986946
>10	0.32665197548798874	3.83014467935295
>50	0.00580483006328833	0.297063814496919
>100	0.0013895259864958858	0.16730277224372148
>500	4.868379317473125E-5	0.023390294386344466
>1k	6.720775305263167E-5	0.10263152031516798
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.324449642439334E-6
2	0.0	1.2648899284878668E-5	0.0	6.324449642439334E-6	6.324449642439334E-6
3	0.0	1.2648899284878668E-5	0.0	9.486674463659E-6	6.324449642439334E-6
4	0.0	2.8460023390977003E-5	0.0	1.5811124106098335E-5	6.324449642439334E-6
5	0.0	3.4784473033416336E-5	0.0	1.8973348927318E-5	6.324449642439334E-6
6	0.0	3.4784473033416336E-5	0.0	1.8973348927318E-5	9.486674463659E-6
7	0.0	3.7946697854636E-5	0.0	1.8973348927318E-5	9.486674463659E-6
8	0.0	4.743337231829501E-5	0.0	1.8973348927318E-5	9.486674463659E-6
9	0.0	1.0119119427902935E-4	0.0	1.8973348927318E-5	1.2648899284878668E-5
10-11	0.0	1.0119119427902935E-4	0.0	3.6365585444026165E-5	1.2648899284878668E-5
12-13	0.0	1.0435341910024901E-4	0.0	4.585225990768517E-5	1.2648899284878668E-5
14-15	0.0	1.7234125275647185E-4	0.0	5.375782196073434E-5	1.7392236516708168E-5
16-17	0.0	1.7392236516708168E-4	0.0	6.324449642439334E-5	2.5297798569757337E-5
18-19	0.0	1.8499015204135051E-4	0.0	6.640672124561301E-5	3.162224821219667E-5
20-21	0.0	1.9605793891561935E-4	0.0	8.06367329411015E-5	3.162224821219667E-5
22-23	0.0	2.2768018712781603E-4	0.0	1.0435341910024901E-4	4.2690035086465505E-5
24-25	0.0	2.6878910980367165E-4	0.0	1.2648899284878668E-4	5.375782196073434E-5
26-27	3.162224821219667E-6	2.8460023390977E-4	0.0	1.4862456659732432E-4	5.375782196073434E-5
28-29	6.324449642439334E-6	2.877624587309897E-4	0.0	3.3519583104928466E-4	5.6920046781954006E-5
30-31	6.324449642439334E-6	3.6049362961904205E-4	0.0	8.980718492263855E-4	6.166338401378351E-5
32-33	6.324449642439334E-6	3.636558544402617E-4	0.0	0.0021582184404824226	6.640672124561301E-5
34-35	9.486674463659002E-6	4.110892267585567E-4	0.0	0.003663437455382984	6.956894606683267E-5
36-37	1.4230011695488502E-5	4.601037114874615E-4	0.0	0.005897549291574679	6.956894606683267E-5
38-39	1.5811124106098335E-5	4.680092735405107E-4	0.0	0.009527783386334858	6.956894606683267E-5
40-41	1.8973348927318E-5	5.217670955012451E-4	0.0	0.013546971134105053	6.956894606683267E-5
42-43	2.5297798569757337E-5	5.470648940710024E-4	0.0	0.017268909748680603	7.273117088805233E-5
44-45	2.5297798569757337E-5	6.245394021908842E-4	0.0	0.021782985680971676	7.5893395709272E-5
46-47	2.5297798569757337E-5	6.782972241516185E-4	0.0	0.027014886647679613	8.06367329411015E-5
48-49	2.687891098036717E-5	6.846216737940579E-4	0.0	0.03221200314135414	8.538007017293101E-5
50-51	2.8460023390977003E-5	7.952995425367462E-4	0.0	0.03717669611066901	8.538007017293101E-5
52-53	2.8460023390977003E-5	8.000428797685757E-4	0.0	0.041901059993571195	8.854229499415068E-5
54-55	2.8460023390977003E-5	8.063673294110151E-4	0.0	0.04642778482514715	9.644785704719984E-5
56-57	2.8460023390977003E-5	8.221784535171134E-4	0.0	0.05113791869635385	1.0119119427902935E-4
58-59	2.8460023390977003E-5	8.269217907489429E-4	0.0	0.05579271363318919	1.0277230668963918E-4
60-61	2.8460023390977003E-5	8.490573644974807E-4	0.0	0.06016132722370417	1.0435341910024901E-4
62-63	2.8460023390977003E-5	8.617062637823593E-4	0.0	0.0649094077927655	1.1067786874268834E-4
64-65	3.162224821219667E-5	9.866141442205361E-4	1.5811124106098336E-6	0.06941715927541414	1.1384009356390801E-4
66-67	3.4784473033416336E-5	0.0010040063807372442	3.162224821219667E-6	0.07461901910632049	1.185834307957375E-4
68-69	3.4784473033416336E-5	0.0010403719661812704	3.162224821219667E-6	0.07961059098661571	1.2016454320634735E-4
70-71	3.4784473033416336E-5	0.0010704131019828571	3.162224821219667E-6	0.08369302323081032	1.2332676802756702E-4
72-73	4.26900350864655E-5	0.001083062001267736	3.162224821219667E-6	0.0869390470097923	1.3913789213366534E-4
74-75	4.585225990768517E-5	0.0011115220246587128	3.162224821219667E-6	0.0896395870071139	1.3913789213366534E-4
76-77	4.743337231829501E-5	0.0011589553969770078	3.162224821219667E-6	0.09227055805836866	1.4071900454427517E-4
78-79	4.743337231829501E-5	0.0011905776451892045	3.162224821219667E-6	0.09395128055084692	1.42300116954885E-4
80-81	4.743337231829501E-5	0.0011937398700104245	3.162224821219667E-6	0.09454103548000438	1.42300116954885E-4
82-83	4.743337231829501E-5	0.0011953209824210342	3.162224821219667E-6	0.09499323362943879	1.51786791418544E-4
84-85	4.743337231829501E-5	0.0012048076568846931	3.162224821219667E-6	0.09522249492897722	1.51786791418544E-4
86-87	4.743337231829501E-5	0.0012174565561695718	3.162224821219667E-6	0.09532368612325626	1.51786791418544E-4
88	4.743337231829501E-5	0.001290187727057624	3.162224821219667E-6	0.09532842946048808	1.51786791418544E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	48475	0.0	50.9338	1
GTATCAA	88480	0.0	37.84554	1
ATCAACG	107640	0.0	30.579596	3
TCAACGC	108000	0.0	30.473696	4
CAACGCA	110280	0.0	29.832008	5
TATCAAC	111780	0.0	29.719189	2
AACGCAG	112570	0.0	29.215569	6
ACGCAGA	127235	0.0	25.836943	7
CGCAGAG	129760	0.0	25.334301	8
GTGGTAT	23690	0.0	24.179182	1
TGGTATC	22695	0.0	23.521399	2
GCAGAGT	148610	0.0	22.060297	9
GAGTACT	100470	0.0	20.637005	12-13
GTACATG	91110	0.0	20.563107	1
TACATGG	89150	0.0	20.361624	2
ACATGGG	88800	0.0	19.645786	3
CAGAGTA	146895	0.0	19.448997	10-11
AGAGTAC	137525	0.0	18.995037	10-11
GTACTTT	110550	0.0	18.759436	14-15
AGTACTT	108020	0.0	17.824118	12-13
>>END_MODULE