FastQCFastQC Report
Fri 27 May 2016
SRR2139146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8012598
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142700.051.8053051
GTATCAA263150.039.5398181
ATCAACG326100.031.3777353
TCAACGC330950.030.9961414
TATCAAC334400.030.8183122
CAACGCA334850.030.5577985
AACGCAG342000.030.046826
CTTATAC60800.029.6697271
ACGCAGA382900.026.7600967
CGCAGAG385850.026.5556688
CTCGTAT96950.026.3553589
CGCTACG2301.09121174E-725.2251394
TCTCGTA100850.025.1649728
ATCTCGT102850.024.717437
TATACAC90150.024.3664883
AATCTCG105700.024.1311346
GCAGAGT428300.023.8631979
GTGGTAT66900.022.4488431
TACATGG257900.021.6198182
GAGTACT300150.021.26731512-13
Produced by FastQC (version 0.11.5)