##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139146_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8012598
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63936041718304	33.0	33.0	33.0	33.0	33.0
2	32.67300930359916	33.0	33.0	33.0	33.0	33.0
3	32.70877560561506	33.0	33.0	33.0	33.0	33.0
4	36.6435872609608	37.0	37.0	37.0	37.0	37.0
5	36.65358638983261	37.0	37.0	37.0	37.0	37.0
6	36.634186315100294	37.0	37.0	37.0	37.0	37.0
7	36.61402206874724	37.0	37.0	37.0	37.0	37.0
8	36.57387391704913	37.0	37.0	37.0	37.0	37.0
9	36.60041599491201	37.0	37.0	37.0	37.0	37.0
10-11	36.5996160046966	37.0	37.0	37.0	37.0	37.0
12-13	36.592493782915355	37.0	37.0	37.0	37.0	37.0
14-15	38.72195341635759	40.0	37.0	40.0	37.0	40.0
16-17	38.66594449141215	40.0	37.0	40.0	37.0	40.0
18-19	38.706665179009356	40.0	37.0	40.0	37.0	40.0
20-21	38.70654677924025	40.0	37.0	40.0	37.0	40.0
22-23	38.70879370657799	40.0	37.0	40.0	37.0	40.0
24-25	38.705845868181385	40.0	37.0	40.0	37.0	40.0
26-27	38.62789415373112	40.0	37.0	40.0	37.0	40.0
28-29	38.53121492623964	40.0	37.0	40.0	37.0	40.0
30-31	38.36080358627642	40.0	37.0	40.0	37.0	40.0
32-33	38.25794014995692	40.0	37.0	40.0	37.0	40.0
34-35	38.221176572798285	40.0	37.0	40.0	37.0	40.0
36-37	38.20261838573363	40.0	37.0	40.0	37.0	40.0
38-39	38.156135435830194	40.0	37.0	40.0	37.0	40.0
40-41	38.14013285276009	40.0	37.0	40.0	37.0	40.0
42-43	38.10576975245992	40.0	37.0	40.0	37.0	40.0
44-45	38.00290292684679	40.0	37.0	40.0	33.0	40.0
46-47	37.92245677591523	40.0	37.0	40.0	33.0	40.0
48-49	37.857670639264796	40.0	37.0	40.0	33.0	40.0
50-51	37.8160506142966	40.0	37.0	40.0	33.0	40.0
52-53	37.75778046770601	40.0	37.0	40.0	33.0	40.0
54-55	37.65266390151072	40.0	37.0	40.0	33.0	40.0
56-57	37.48125266882628	40.0	37.0	40.0	33.0	40.0
58-59	37.25944028619614	38.5	37.0	40.0	33.0	40.0
60-61	37.106575024303744	37.0	37.0	40.0	33.0	40.0
62-63	36.9851345599774	37.0	37.0	40.0	33.0	40.0
64-65	36.8189033719389	37.0	37.0	40.0	33.0	40.0
66-67	36.61778446996253	37.0	37.0	40.0	33.0	40.0
68-69	36.42078577228456	37.0	37.0	40.0	33.0	40.0
70-71	36.208425682266736	37.0	37.0	40.0	33.0	40.0
72-73	35.917146366550824	37.0	37.0	37.0	33.0	40.0
74-75	35.58816777317477	37.0	33.0	37.0	33.0	40.0
76-77	33.333661082129076	35.0	33.0	37.0	27.0	37.0
78-79	35.091262038021	37.0	33.0	37.0	33.0	37.0
80-81	35.330026547912055	37.0	33.0	37.0	33.0	37.0
82-83	35.18400333972238	37.0	33.0	37.0	33.0	37.0
84-85	35.01514265669876	37.0	33.0	37.0	33.0	37.0
86-87	34.896969002987916	37.0	33.0	37.0	33.0	37.0
88-89	34.78908489825427	37.0	33.0	37.0	33.0	37.0
90-91	34.71748922475885	37.0	33.0	37.0	33.0	37.0
92-93	34.64387175317563	37.0	33.0	37.0	33.0	37.0
94-95	34.640486206625354	37.0	33.0	37.0	33.0	37.0
96-97	34.679351458419944	37.0	33.0	37.0	33.0	37.0
98-99	34.69142586813095	37.0	33.0	37.0	33.0	37.0
100	34.61494606637002	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	7.0
19	11.0
20	38.0
21	210.0
22	808.0
23	2489.0
24	5957.0
25	11906.0
26	21456.0
27	35095.0
28	52776.0
29	75044.0
30	103223.0
31	138406.0
32	185653.0
33	251433.0
34	352031.0
35	527553.0
36	961840.0
37	2423740.0
38	2783718.0
39	79202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56959347886834	20.52142395280831	14.780551174606686	23.12843139371667
2	14.078288650882568	20.28315727058666	43.280409466165636	22.358144612365134
3	17.825306826238734	26.883942871456036	30.567779709874337	24.722970592430894
4	12.189580981047717	17.929180763739897	39.47192152556781	30.40931672964458
5	12.647009122384526	38.28781875741177	35.81110646010195	13.254065660101755
6	28.89716918282934	38.808823305499665	18.702460799855427	13.59154671181557
7	25.6800603250032	33.4516719795502	23.223416424984755	17.64485127046184
8	23.8391842446108	37.026480050540414	21.456274232152918	17.678061472695873
9	24.985291911562268	17.15436116974794	21.731341070648995	36.1290058480408
10-11	23.946109613885532	27.146501047475486	28.802462072850776	20.1049272657882
12-13	24.97005114333614	24.442148719070115	29.201364654383077	21.38643548321067
14-15	21.761257914602357	25.96131849446925	27.528494939758975	24.748928651169418
16-17	21.04079101435015	28.947801948881	27.91185206096699	22.099554975801855
18-19	20.897691360530004	27.724777905992536	30.27768271913804	21.099848014339422
20-21	21.798606319481916	26.940616844068145	30.43191340801733	20.82886342843261
22-23	21.252505178236884	26.98859180852197	30.621292940017202	21.137610073223943
24-25	20.992321598876153	27.071774165509982	30.740313768733397	21.19559046688047
26-27	21.263595134848654	27.350504093338813	30.29765683889044	21.088243932922094
28-29	20.778441770715496	27.58271618559684	30.64807400362376	20.99076804006391
30-31	21.579062530572457	27.468396680922762	30.09501325233889	20.857527536165886
32-33	20.882870911579175	27.985762806949992	30.057595757819822	21.07377052365101
34-35	20.997264336951385	28.037446747147342	30.163151836553347	20.802137079347922
36-37	21.164987525004666	27.80105352528362	30.045008555425984	20.988950394285734
38-39	21.043690717983175	27.699022570447195	30.150599437533465	21.106687274036158
40-41	21.350751668745904	27.59154897309834	29.989544991094768	21.068154367060984
42-43	21.452167618719415	27.561369325183577	29.74179575660886	21.244667299488153
44-45	21.423379931493976	28.002547508230602	29.267482433178515	21.30659012709691
46-47	21.589670226016242	27.724393991627615	28.779672817589656	21.906262964766483
48-49	21.7292855706492	27.8261133990449	28.70552575519526	21.739075275110643
50-51	21.355578072283958	28.04640024326484	28.67701988995212	21.92100179449908
52-53	21.563207823021877	28.094782276002427	28.535516275457823	21.806493625517874
54-55	21.731913353717445	28.031511941921227	28.323218329969635	21.91335637439169
56-57	21.859471618373423	28.17892811203007	28.102910342124726	21.85868992747178
58-59	21.581037942467248	28.362822404410554	28.496911747254373	21.55922790586783
60-61	21.76697797525199	28.510663465496528	28.087459642228023	21.634898917023452
62-63	21.658364076215367	28.581775612729132	27.93091872893672	21.82894158211878
64-65	21.659548647448336	28.566349823601684	27.942488745537926	21.83161278341206
66-67	21.824795077206293	28.44621254768112	27.81636853735777	21.912623837754815
68-69	21.8074089005604	28.198008970817508	28.019212206457205	21.975369922164884
70-71	21.802867448106877	27.82243066281777	28.114546682342116	22.260155206733234
72-73	21.99117428656607	27.896913181176004	28.13994565052772	21.97196688173021
74-75	21.69588110819902	28.11107303008725	28.099078226684814	22.09396763502891
76-77	21.829870512473693	28.043056254706798	27.899228782673035	22.22784445014647
78-79	21.87964938186067	27.98458528327919	27.95077658649688	22.18498874836326
80-81	21.793225786505165	27.882138345181893	28.050937028834365	22.273698839478577
82-83	22.040647540559302	27.734822363740548	28.14634152890808	22.078188566792072
84-85	22.389418909158966	27.67844993821132	27.91755050118823	22.01458065144148
86-87	21.838100635945064	27.89844076142766	28.026970530787114	22.236488071840157
88-89	22.19624489332693	27.701243858654134	27.956889764325897	22.14562148369303
90-91	22.25765111715526	27.61701683302832	28.062357912067316	22.062974137749105
92-93	22.18241300064723	27.662930644378225	28.000133504870707	22.154522850103838
94-95	22.008688547474232	27.69756710211316	28.0748700318328	22.218874318579807
96-97	22.04802602346405	27.822539680379162	28.19486570868916	21.93456858746763
98-99	23.189312522698792	29.74639792540257	28.325667814118642	18.73862173778
100	22.316545429912	30.682934438226837	22.05854809971101	24.94197203215015
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1081.0
1	1151.0
2	2405.0
3	3819.5
4	5719.0
5	10741.0
6	14887.0
7	17026.5
8	17677.5
9	16289.5
10	14133.5
11	12118.5
12	13261.5
13	18880.0
14	32512.5
15	52135.0
16	68805.5
17	78457.0
18	79976.0
19	74483.0
20	64794.5
21	54128.5
22	46438.0
23	44369.0
24	47912.5
25	57024.5
26	72125.0
27	89603.0
28	102992.5
29	116754.0
30	131207.5
31	144839.5
32	164420.5
33	185241.0
34	206053.5
35	227302.5
36	252193.0
37	277397.0
38	288093.5
39	294638.0
40	307410.0
41	313226.0
42	312577.0
43	314906.5
44	318302.5
45	324814.0
46	334541.5
47	341567.5
48	337748.5
49	324671.5
50	311041.0
51	293849.5
52	273155.0
53	252082.5
54	230546.0
55	208563.0
56	184951.0
57	161105.0
58	138823.5
59	119517.5
60	97688.0
61	75090.5
62	56847.5
63	41463.5
64	29593.0
65	21023.0
66	15526.0
67	11767.0
68	8798.0
69	6522.5
70	4888.0
71	3611.5
72	2652.5
73	1785.0
74	1195.0
75	797.5
76	547.5
77	361.0
78	230.0
79	154.0
80	112.0
81	80.0
82	63.0
83	45.5
84	24.0
85	10.5
86	7.5
87	6.5
88	4.5
89	3.5
90	3.0
91	2.5
92	2.0
93	2.0
94	1.5
95	1.5
96	2.0
97	2.5
98	2.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.2480346574232228E-5
2	2.3712658491041233E-4
3	1.2480346574232228E-5
4	1.2480346574232228E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.00756309002398473
14-15	4.36812130098128E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.240906998503181E-6
24-25	6.866132390018292E-5
26-27	0.0
28-29	1.249529161792722E-5
30-31	1.2510466569093369E-5
32-33	1.2533621439511489E-5
34-35	0.0028638947222251357
36-37	0.032202070988208535
38-39	0.13763034527149237
40-41	0.18644813593904228
42-43	0.05820977583406357
44-45	0.006968479844493956
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.012391980796918627
64-65	0.0754277166809694
66-67	0.19860654260921543
68-69	0.21443174608148013
70-71	0.08633824850078473
72-73	0.005872345890295029
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	121.0
20-21	562.0
22-23	1165.0
24-25	2277.0
26-27	3940.0
28-29	7664.0
30-31	14330.0
32-33	16404.0
34-35	22177.0
36-37	37938.0
38-39	44256.0
40-41	46959.0
42-43	44821.0
44-45	55536.0
46-47	57113.0
48-49	44137.0
50-51	42753.0
52-53	34514.0
54-55	40059.0
56-57	50269.0
58-59	48011.0
60-61	34632.0
62-63	51037.0
64-65	65968.0
66-67	79476.0
68-69	68332.0
70-71	66693.0
72-73	63173.0
74-75	61786.0
76-77	58066.0
78-79	63801.0
80-81	65330.0
82-83	65529.0
84-85	71989.0
86-87	75806.0
88-89	86039.0
90-91	90864.0
92-93	91079.0
94-95	100073.0
96-97	264185.0
98-99	507787.0
100-101	5365947.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.72719840537448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.25248542705191	65.41184363912707
2	9.074106739864067	13.92461576362192
3	2.8145732860330708	6.478629688317787
4	1.2196658895700996	3.7432618678925027
5	0.6029198765630505	2.3130176495798525
6	0.336316242481281	1.5482761838286765
7	0.19339197013069082	1.0386896843556637
8	0.13145719607329479	0.8069073891943923
9	0.09463460845837195	0.6534943541280391
>10	0.27426931458141096	3.0697418688170903
>50	0.003683569803266692	0.19225372348348205
>100	0.00194152219136934	0.28849361538127566
>500	2.0788395926477256E-4	0.11338042957836451
>1k	3.464732388702986E-4	0.41739414269391434
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	2.4960693148464456E-5	0.0	0.0	0.0	0.0
4	2.4960693148464456E-5	0.0	0.0	0.0	0.0
5	2.4960693148464456E-5	0.0	0.0	0.0	0.0
6	2.4960693148464456E-5	0.0	0.0	0.0	0.0
7	2.4960693148464456E-5	0.0	0.0	0.0	0.0
8	2.4960693148464456E-5	0.0	0.0	0.0	0.0
9	2.4960693148464456E-5	0.0	0.0	0.0	0.0
10-11	2.4960693148464456E-5	0.0	0.0	0.0	0.0
12-13	2.4960693148464456E-5	0.0	0.0	0.0	0.0
14-15	2.4960693148464456E-5	0.0	0.0	0.0	0.0
16-17	2.4960693148464456E-5	0.0	0.0	0.0	0.0
18-19	2.4960693148464456E-5	0.0	0.0	1.2480346574232228E-5	0.0
20-21	2.4960693148464456E-5	0.0	0.0	1.8720519861348343E-5	0.0
22-23	3.120086643558057E-5	0.0	0.0	3.120086643558057E-5	0.0
24-25	4.3681213009812795E-5	0.0	0.0	6.240173287116114E-5	0.0
26-27	4.992138629692891E-5	0.0	0.0	8.112225273250948E-5	0.0
28-29	4.992138629692891E-5	0.0	0.0	2.4336675819752843E-4	0.0
30-31	4.992138629692891E-5	0.0	0.0	6.05296808850263E-4	0.0
32-33	4.992138629692891E-5	0.0	0.0	0.001410279162888242	0.0
34-35	4.992138629692891E-5	0.0	0.0	0.0023837461956783554	0.0
36-37	4.992138629692891E-5	0.0	0.0	0.0036505013729629266	0.0
38-39	6.240173287116114E-5	0.0	0.0	0.0060217672220670496	0.0
40-41	6.240173287116114E-5	0.0	0.0	0.008761203295111025	0.0
42-43	6.240173287116114E-5	0.0	0.0	0.011463198328432301	0.0
44-45	6.240173287116114E-5	0.0	0.0	0.014077830935733953	0.0
46-47	6.240173287116114E-5	0.0	0.0	0.016499018171135006	0.0
48-49	6.240173287116114E-5	0.0	0.0	0.01947558082908939	0.0
50-51	6.864190615827725E-5	0.0	0.0	0.023082400989042504	0.0
52-53	7.488207944539337E-5	0.0	0.0	0.027132273452380864	0.0
54-55	8.112225273250948E-5	0.0	0.0	0.030583089280156073	0.0
56-57	8.736242601962559E-5	0.0	0.0	0.033403647605932556	0.0
58-59	9.984277259385782E-5	0.0	0.0	0.036155564025550764	0.0
60-61	9.984277259385782E-5	0.0	0.0	0.03869531455340702	0.0
62-63	9.984277259385782E-5	0.0	0.0	0.04096049745663018	0.0
64-65	9.984277259385782E-5	0.0	0.0	0.04376857543583242	0.0
66-67	9.984277259385782E-5	0.0	0.0	0.04780596755259655	0.0
68-69	1.1232311916809004E-4	0.0	0.0	0.052417455611775354	0.0
70-71	1.1232311916809004E-4	0.0	0.0	0.05676061621960817	0.0
72-73	1.2480346574232228E-4	0.0	0.0	0.06081672885623365	0.0
74-75	1.2480346574232228E-4	0.0	0.0	0.06340640077038684	0.0
76-77	1.2480346574232228E-4	0.0	0.0	0.06586502904551059	0.0
78-79	1.2480346574232228E-4	0.0	0.0	0.06759355704604175	0.0
80-81	1.2480346574232228E-4	0.0	0.0	0.06818013333503066	0.0
82-83	1.3104363902943837E-4	0.0	0.0	0.06857950442540608	0.0
84-85	1.372838123165545E-4	0.0	0.0	0.0686980677178613	0.0
86-87	1.372838123165545E-4	0.0	0.0	0.06880415066374226	0.0
88	1.372838123165545E-4	0.0	0.0	0.0688166310103165	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14270	0.0	51.805305	1
GTATCAA	26315	0.0	39.539818	1
ATCAACG	32610	0.0	31.377735	3
TCAACGC	33095	0.0	30.996141	4
TATCAAC	33440	0.0	30.818312	2
CAACGCA	33485	0.0	30.557798	5
AACGCAG	34200	0.0	30.04682	6
CTTATAC	6080	0.0	29.669727	1
ACGCAGA	38290	0.0	26.760096	7
CGCAGAG	38585	0.0	26.555668	8
CTCGTAT	9695	0.0	26.355358	9
CGCTACG	230	1.09121174E-7	25.22513	94
TCTCGTA	10085	0.0	25.164972	8
ATCTCGT	10285	0.0	24.71743	7
TATACAC	9015	0.0	24.366488	3
AATCTCG	10570	0.0	24.131134	6
GCAGAGT	42830	0.0	23.863197	9
GTGGTAT	6690	0.0	22.448843	1
TACATGG	25790	0.0	21.619818	2
GAGTACT	30015	0.0	21.267315	12-13
>>END_MODULE