FastQCFastQC Report
Fri 27 May 2016
SRR2139145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9886403
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC146850.051.6571961
TATACAC176450.044.740673
GGTATCA269550.043.9465681
TTATACA192350.040.366732
GTATCAA467050.034.2752881
TACACAT267250.029.5549165
ATACACA280600.028.8000554
ACACATC275350.027.907936
ATCAACG586650.026.8930343
TATCAAC595600.026.7957382
AACGCAG598650.026.513876
CAACGCA595400.026.5046375
TCAACGC603800.026.2166144
ACATCTC301950.025.4631168
CACATCT304500.025.1031557
ACGCAGA658400.023.972267
CGCAGAG659900.023.9362328
CATCTCC338350.022.6157349
GCAGAGT718250.021.895549
GTACTAG32550.021.5835571
Produced by FastQC (version 0.11.5)