##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139145_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9886403
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67956030115301	33.0	33.0	33.0	33.0	33.0
2	32.692725453332216	33.0	33.0	33.0	33.0	33.0
3	32.65163356177166	33.0	33.0	33.0	33.0	33.0
4	36.60278283213824	37.0	37.0	37.0	37.0	37.0
5	36.633699840073284	37.0	37.0	37.0	37.0	37.0
6	36.608556013749386	37.0	37.0	37.0	37.0	37.0
7	36.5079846532657	37.0	37.0	37.0	37.0	37.0
8	36.551897287618154	37.0	37.0	37.0	37.0	37.0
9	36.604367938470645	37.0	37.0	37.0	37.0	37.0
10-11	36.588333087372625	37.0	37.0	37.0	37.0	37.0
12-13	36.57143715464563	37.0	37.0	37.0	37.0	37.0
14-15	38.644200777573	40.0	37.0	40.0	37.0	40.0
16-17	38.58188746705956	40.0	37.0	40.0	37.0	40.0
18-19	38.633431542290964	40.0	37.0	40.0	37.0	40.0
20-21	38.64982959154057	40.0	37.0	40.0	37.0	40.0
22-23	38.62754156600485	40.0	37.0	40.0	37.0	40.0
24-25	38.644132874212445	40.0	37.0	40.0	37.0	40.0
26-27	38.55216764164987	40.0	37.0	40.0	37.0	40.0
28-29	38.432299017746814	40.0	37.0	40.0	37.0	40.0
30-31	38.25393892666003	40.0	37.0	40.0	37.0	40.0
32-33	38.155992465772755	40.0	37.0	40.0	37.0	40.0
34-35	38.12477913801135	40.0	37.0	40.0	37.0	40.0
36-37	38.09231057925858	40.0	37.0	40.0	37.0	40.0
38-39	38.035396580426095	40.0	37.0	40.0	35.0	40.0
40-41	38.02183502647407	40.0	37.0	40.0	35.0	40.0
42-43	37.98562892239963	40.0	37.0	40.0	33.0	40.0
44-45	37.87564704517394	40.0	37.0	40.0	33.0	40.0
46-47	37.78891009681381	40.0	37.0	40.0	33.0	40.0
48-49	37.72676425975226	40.0	37.0	40.0	33.0	40.0
50-51	37.65113177379126	40.0	37.0	40.0	33.0	40.0
52-53	37.602706204899576	40.0	37.0	40.0	33.0	40.0
54-55	37.51498685740735	40.0	37.0	40.0	33.0	40.0
56-57	37.33582066192497	40.0	37.0	40.0	33.0	40.0
58-59	37.1285395705672	37.0	37.0	40.0	33.0	40.0
60-61	36.94732289933914	37.0	37.0	40.0	33.0	40.0
62-63	36.825915955670034	37.0	37.0	40.0	33.0	40.0
64-65	36.69966447720397	37.0	37.0	40.0	33.0	40.0
66-67	36.5270387069794	37.0	37.0	40.0	33.0	40.0
68-69	36.35506654389632	37.0	37.0	40.0	33.0	40.0
70-71	36.16363555523327	37.0	37.0	38.5	33.0	40.0
72-73	35.886532169309994	37.0	37.0	37.0	33.0	40.0
74-75	35.5493800700008	37.0	33.0	37.0	33.0	40.0
76-77	33.35624268945193	35.0	33.0	37.0	27.0	37.0
78-79	35.041666032883036	37.0	33.0	37.0	33.0	37.0
80-81	35.27328866594259	37.0	33.0	37.0	33.0	37.0
82-83	35.152553353233245	37.0	33.0	37.0	33.0	37.0
84-85	34.99304346474291	37.0	33.0	37.0	33.0	37.0
86-87	34.88771938994264	37.0	33.0	37.0	33.0	37.0
88-89	34.80365315405038	37.0	33.0	37.0	33.0	37.0
90-91	34.71954568238542	37.0	33.0	37.0	33.0	37.0
92-93	34.7053306686543	37.0	33.0	37.0	33.0	37.0
94-95	34.67745274526129	37.0	33.0	37.0	33.0	37.0
96-97	34.68554469561785	37.0	33.0	37.0	33.0	37.0
98-99	34.707373586495834	37.0	33.0	37.0	33.0	37.0
100	34.66315522562806	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	3.0
19	17.0
20	60.0
21	280.0
22	1034.0
23	2985.0
24	7175.0
25	14269.0
26	25554.0
27	41607.0
28	62365.0
29	88599.0
30	122518.0
31	167110.0
32	227147.0
33	312039.0
34	441090.0
35	664915.0
36	1221535.0
37	3000563.0
38	3361632.0
39	123903.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.694673350325026	21.124247223610773	14.9980043295832	23.183075096481005
2	14.119238199046322	20.702306874864586	43.43712075281079	21.7413341732783
3	17.26604886186097	27.512688640417416	31.876581591563845	23.344680906157773
4	11.627678097653726	18.917600154232044	38.93656155191747	30.51816019619676
5	12.285025431901312	38.148519552411486	35.99157711774213	13.574877897945075
6	27.731521767825974	38.922649622921504	18.93632092480956	14.409507684442968
7	24.57298170021999	33.968147970500496	23.207095644391597	18.251774684887923
8	23.750680606485496	36.521493206376476	21.707864832133588	18.019961355004444
9	24.88815193958814	16.456551487937524	21.80293479842972	36.85236177404461
10-11	23.579445426208096	26.922789815466757	28.9227487489636	20.575016009361544
12-13	24.779486247819847	24.572496974947242	29.153856176013665	21.49416060121925
14-15	21.87515014308035	25.948107719258463	27.437749604178585	24.7389925334826
16-17	20.504110544552958	29.24481735166976	28.127090307769166	22.123981796008113
18-19	20.393114664656096	27.444046130832415	31.087985185309563	21.07485401920193
20-21	21.28525845261378	26.54921279684903	31.05011688016176	21.11541187037543
22-23	20.98740858473075	27.058348103315737	30.939575238591345	21.01466807336217
24-25	20.886576246327262	27.174143202579653	31.23192724465449	20.70735330643859
26-27	20.95603935231916	27.44210634165049	31.124451193004106	20.47740311302625
28-29	20.31934180817298	27.33151999870283	31.15201304465911	21.197125148465084
30-31	20.644330214919083	27.304740453086	31.322491757209615	20.7284375747853
32-33	19.987447327976188	27.217142321078434	31.667812088222142	21.12759826272324
34-35	20.034453551423155	27.491252267859473	31.263005166497347	21.21128901422002
36-37	20.341729675137966	27.60841068431753	31.377340960914385	20.672518679630116
38-39	20.567127278672512	27.253645263952652	31.170100837243115	21.00912662013172
40-41	20.613472290611572	27.28432120385942	31.16539547464881	20.936811030880197
42-43	21.085389639174515	27.375177598830547	30.72269806913036	20.81673469286458
44-45	20.63863214961062	28.225441858056833	30.045379698599806	21.09054629373275
46-47	21.413198343120253	27.67279275122714	29.022233286044184	21.891775619608424
48-49	21.496087016401418	27.66189898654866	28.91627781990583	21.925736177144092
50-51	20.962073665341528	27.905081004366615	29.095858367622906	22.03698696266895
52-53	21.01969045363153	28.27682995716326	29.15702308646859	21.546456502736618
54-55	20.976267666603434	28.41991068461342	28.585037176709537	22.01878447207361
56-57	21.837351963751892	28.574938752335328	27.845823678926212	21.741885604986567
58-59	20.975518583500747	29.191662029669025	28.740049960784724	21.09276942604551
60-61	21.587967447565727	29.62804630541375	27.596066121279673	21.18792012574085
62-63	21.21772950403485	29.798175948089558	27.497635390411574	21.486459157464026
64-65	21.140508683408974	29.59840889279692	27.681840192436418	21.579242231357693
66-67	21.418697343457985	29.00115251442008	27.719063998433196	21.861086143688734
68-69	21.453637869476182	28.565397378263725	28.0077256359736	21.97323911628649
70-71	21.464299408942463	28.159530499700384	28.09947285513773	22.276697236219423
72-73	21.7239416621673	28.232653102354504	28.16041831295913	21.88298692251907
74-75	21.675514065839252	28.336943084495854	28.063399745770024	21.924143103894874
76-77	21.548514390216926	28.199698887068227	27.95160528561117	22.300181437103674
78-79	21.746040522521156	28.047224005691884	28.017777863200845	22.18895760858612
80-81	21.602321583118776	28.061838671970314	28.14192474888398	22.193914996026933
82-83	22.02717882876485	27.69204371171256	28.33115445694404	21.94962300257855
84-85	22.332011993845615	27.838254974546427	27.8921705489252	21.93756248268275
86-87	21.658783609875396	28.03362763348114	28.098915563244812	22.20867319339865
88-89	22.176420531248382	27.5870142471949	28.00570669454876	22.230858527007957
90-91	22.179297429141155	27.443000520099144	28.174152221249905	22.203549829509804
92-93	22.188967420557518	27.606227024701397	28.045728715665142	22.159076839075944
94-95	21.806579451468934	27.51827848909789	28.23869512662337	22.436446932809805
96-97	21.93959496198124	27.635424789128265	28.24427257393737	22.180707674953123
98-99	23.18318746760887	29.751433804349414	28.17262892636352	18.892749801678193
100	22.439071279992756	30.004317463798408	21.95935308016957	25.59725817603927
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2298.0
1	2499.0
2	5198.0
3	8163.0
4	12267.5
5	22908.0
6	31037.5
7	34262.5
8	34282.0
9	30816.0
10	26816.5
11	23194.0
12	25305.5
13	35803.0
14	60902.0
15	97222.0
16	127507.5
17	143538.0
18	144931.5
19	134056.0
20	115837.0
21	95467.0
22	79330.0
23	72442.0
24	74706.5
25	84688.5
26	99732.5
27	115427.0
28	130117.0
29	149094.5
30	168354.0
31	186901.5
32	210977.5
33	234640.5
34	259466.0
35	284639.5
36	309721.5
37	340825.0
38	358216.0
39	361531.5
40	370688.0
41	378012.5
42	387945.5
43	406026.5
44	425889.5
45	449168.5
46	474715.0
47	497375.5
48	518006.5
49	502832.5
50	461709.0
51	434708.0
52	402161.5
53	357200.5
54	307824.0
55	257254.0
56	213152.0
57	172456.5
58	136001.5
59	111639.0
60	88323.0
61	66099.5
62	49858.0
63	37219.5
64	26903.0
65	18698.5
66	13762.0
67	10694.5
68	8643.0
69	6796.5
70	4820.5
71	3364.5
72	2420.5
73	1923.5
74	1633.0
75	1179.5
76	759.0
77	500.5
78	293.5
79	162.0
80	109.5
81	73.5
82	55.5
83	39.5
84	27.5
85	19.5
86	12.5
87	9.0
88	6.5
89	4.5
90	3.5
91	2.5
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0114902255147802E-5
2	4.1471099246105994E-4
3	1.0114902255147802E-5
4	1.0114902255147802E-5
5	1.0114902255147802E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.006321813909467377
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.0115333515443055E-5
22-23	1.0116714000242295E-5
24-25	3.541754093179907E-5
26-27	0.0
28-29	1.0134134389364186E-5
30-31	1.0155439667319984E-5
32-33	1.0191337780731228E-5
34-35	0.0016837533513216875
36-37	0.03578684195940833
38-39	0.11626082795532021
40-41	0.15265483005563835
42-43	0.05404709913634413
44-45	0.001401413801871968
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.009582415618905169
64-65	0.07387459758153349
66-67	0.17823708635978022
68-69	0.19846219251059846
70-71	0.07889205785981594
72-73	0.005837733248484676
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	223.0
20-21	1081.0
22-23	2215.0
24-25	4026.0
26-27	7861.0
28-29	16248.0
30-31	32919.0
32-33	40270.0
34-35	50882.0
36-37	63437.0
38-39	69729.0
40-41	97167.0
42-43	119080.0
44-45	136326.0
46-47	145146.0
48-49	117389.0
50-51	99639.0
52-53	93378.0
54-55	104064.0
56-57	111883.0
58-59	112644.0
60-61	109500.0
62-63	104462.0
64-65	131412.0
66-67	158367.0
68-69	126410.0
70-71	113466.0
72-73	104083.0
74-75	102078.0
76-77	97061.0
78-79	103872.0
80-81	110257.0
82-83	104594.0
84-85	111234.0
86-87	121380.0
88-89	126461.0
90-91	123875.0
92-93	117793.0
94-95	129505.0
96-97	293617.0
98-99	526447.0
100-101	5544922.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.81751945274618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.47067596923608	67.2893350932814
2	8.21438292017377	12.784458053658565
3	2.4586345132045833	5.739745171754724
4	1.1287244453596477	3.5133814593425807
5	0.5820293246989607	2.264603914841505
6	0.3464056751361245	1.6173858218068229
7	0.23143117173550914	1.2606579795949877
8	0.14087164882366723	0.8769825818140923
9	0.1060168546318338	0.742497178286852
>10	0.3170032321254882	3.6192679718952383
>50	0.0030895310730004945	0.16090646781495996
>100	6.529978076664737E-4	0.09075942428035477
>500	8.171599373506774E-5	0.04001888162795549
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0114902255147802E-5
2	0.0	0.0	0.0	0.0	1.0114902255147802E-5
3	0.0	0.0	0.0	0.0	1.0114902255147802E-5
4	0.0	0.0	0.0	0.0	1.0114902255147802E-5
5	0.0	0.0	0.0	0.0	2.0229804510295604E-5
6	0.0	0.0	0.0	0.0	2.0229804510295604E-5
7	0.0	0.0	0.0	0.0	2.0229804510295604E-5
8	0.0	0.0	0.0	0.0	2.0229804510295604E-5
9	0.0	0.0	0.0	0.0	2.0229804510295604E-5
10-11	0.0	0.0	0.0	0.0	2.0229804510295604E-5
12-13	0.0	0.0	0.0	5.057451127573901E-6	7.586176691360852E-5
14-15	0.0	0.0	0.0	1.0114902255147802E-5	1.1126392480662582E-4
16-17	0.0	0.0	0.0	2.0229804510295604E-5	1.4160863157206924E-4
18-19	0.0	0.0	0.0	3.540215789301731E-5	1.5678098495479093E-4
20-21	0.0	0.0	0.0	8.091921804118243E-5	1.6689588720993873E-4
22-23	0.0	0.0	0.0	1.3149372931692144E-4	1.8206824059266045E-4
24-25	0.0	0.0	0.0	2.6804490976141675E-4	1.8206824059266045E-4
26-27	0.0	0.0	0.0	4.501131503540772E-4	1.8712569172023435E-4
28-29	0.0	0.0	0.0	0.0011632137593419972	1.9218314284780825E-4
30-31	0.0	0.0	0.0	0.003079987736692506	1.9218314284780825E-4
32-33	0.0	0.0	0.0	0.006033539195195664	2.0735549623052994E-4
34-35	0.0	0.0	0.0	0.009250078112332666	2.1747039848567777E-4
36-37	0.0	0.0	0.0	0.01373097981136314	2.2252784961325167E-4
38-39	0.0	0.0	0.0	0.01956222096145585	2.2758530074082556E-4
40-41	0.0	0.0	0.0	0.026111620171664053	2.3770020299597336E-4
42-43	0.0	0.0	0.0	0.03177090798341925	2.57930007506269E-4
44-45	0.0	0.0	0.0	0.037951113261314555	2.731023608889907E-4
46-47	0.0	1.0114902255147802E-5	0.0	0.044844419148197784	2.8827471427171234E-4
48-49	0.0	1.0114902255147802E-5	0.0	0.05153542698997805	3.1356196990958186E-4
50-51	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.05746275971149467	3.540215789301731E-4
52-53	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.06300572614731566	4.045960902059121E-4
54-55	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.06795191335008294	4.349407969713555E-4
56-57	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.07245304485362372	4.501131503540772E-4
58-59	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.07673164850755124	4.5517060148165113E-4
60-61	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.0811164586351578	4.652855037367989E-4
62-63	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.08490954698083822	5.158600150125379E-4
64-65	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.08877849709343227	5.209174661401118E-4
66-67	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.09402813136385398	5.310323683952597E-4
68-69	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.10042074958910738	5.360898195228335E-4
70-71	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.1061154395587556	5.360898195228335E-4
72-73	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.11128415461113612	5.462047217779814E-4
74-75	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.11478896824254484	5.563196240331291E-4
76-77	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.11798021990404398	5.563196240331291E-4
78-79	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.12025101546032466	5.61377075160703E-4
80-81	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.12101974803171589	5.765494285434247E-4
82-83	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.1217530784452141	6.372388420743116E-4
84-85	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.1221020425730167	6.524111954570332E-4
86-87	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.12222847885120605	7.080431578603461E-4
88	1.0114902255147802E-5	1.0114902255147802E-5	0.0	0.12224870865571634	7.586176691360851E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	14685	0.0	51.657196	1
TATACAC	17645	0.0	44.74067	3
GGTATCA	26955	0.0	43.946568	1
TTATACA	19235	0.0	40.36673	2
GTATCAA	46705	0.0	34.275288	1
TACACAT	26725	0.0	29.554916	5
ATACACA	28060	0.0	28.800055	4
ACACATC	27535	0.0	27.90793	6
ATCAACG	58665	0.0	26.893034	3
TATCAAC	59560	0.0	26.795738	2
AACGCAG	59865	0.0	26.51387	6
CAACGCA	59540	0.0	26.504637	5
TCAACGC	60380	0.0	26.216614	4
ACATCTC	30195	0.0	25.463116	8
CACATCT	30450	0.0	25.103155	7
ACGCAGA	65840	0.0	23.97226	7
CGCAGAG	65990	0.0	23.936232	8
CATCTCC	33835	0.0	22.615734	9
GCAGAGT	71825	0.0	21.89554	9
GTACTAG	3255	0.0	21.583557	1
>>END_MODULE