FastQCFastQC Report
Fri 27 May 2016
SRR2139144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8890312
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGG655.385673E-5178.3882494
TGCGAGT1650.002190504770.2741694
CTGACGG1700.002465786268.20727594
CAGACGT1750.002766070766.2584994
TTGTGGG1800.003092804264.4179894
AACTAAT1800.003092804264.4179894
AGCACGT1800.003092804264.4179894
ACAATTG1850.003447471762.67695294
TCAGGGG3308.186555E-658.56180694
CACCAGG2200.00684485952.7056294
GCCAGGG3053.9099768E-450.68955694
TATACGG2350.00888382349.3414394
CTTATCG4002.5565212E-548.31348894
CGTACAT1709.4807547E-745.47151694
CTTATGT6001.1920747E-745.09258394
ATATGAT3507.7056844E-444.1723394
CTTGGAT3800.001155013640.6850494
GAGACAG11550.040.15666694
AGCGGCG3850.001231702540.15666294
CTTGGTG6903.5720223E-739.21094594
Produced by FastQC (version 0.11.5)