##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139144_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8890312
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70364144700434	33.0	33.0	33.0	33.0	33.0
2	32.619165559093986	33.0	33.0	33.0	33.0	33.0
3	32.63793734123167	33.0	33.0	33.0	33.0	33.0
4	36.507891286605016	37.0	37.0	37.0	37.0	37.0
5	36.5556342679537	37.0	37.0	37.0	37.0	37.0
6	36.569923755206794	37.0	37.0	37.0	37.0	37.0
7	36.50673947101069	37.0	37.0	37.0	37.0	37.0
8	36.58426329694616	37.0	37.0	37.0	37.0	37.0
9	36.56535856109437	37.0	37.0	37.0	37.0	37.0
10-11	36.55835695080218	37.0	37.0	37.0	37.0	37.0
12-13	36.56156887407326	37.0	37.0	37.0	37.0	37.0
14-15	38.393979761340205	40.0	37.0	40.0	37.0	40.0
16-17	38.462840955413036	40.0	37.0	40.0	37.0	40.0
18-19	38.56252918907683	40.0	37.0	40.0	37.0	40.0
20-21	38.562761829911956	40.0	37.0	40.0	37.0	40.0
22-23	38.548330612453796	40.0	37.0	40.0	37.0	40.0
24-25	38.52058371767984	40.0	37.0	40.0	37.0	40.0
26-27	38.424857850199174	40.0	37.0	40.0	37.0	40.0
28-29	38.36187030878996	40.0	37.0	40.0	37.0	40.0
30-31	38.41184840895468	40.0	37.0	40.0	37.0	40.0
32-33	38.34861279783651	40.0	37.0	40.0	37.0	40.0
34-35	38.20158280481607	40.0	37.0	40.0	37.0	40.0
36-37	38.10951337971704	40.0	37.0	40.0	37.0	40.0
38-39	38.04403386015383	40.0	37.0	40.0	37.0	40.0
40-41	38.26422066571989	40.0	37.0	40.0	37.0	40.0
42-43	38.23977906472241	40.0	37.0	40.0	37.0	40.0
44-45	38.149142368204906	40.0	37.0	40.0	37.0	40.0
46-47	38.0905771623017	40.0	37.0	40.0	37.0	40.0
48-49	37.94297623442955	40.0	37.0	40.0	33.0	40.0
50-51	37.950603642984134	40.0	37.0	40.0	33.0	40.0
52-53	38.00343697989774	40.0	37.0	40.0	33.0	40.0
54-55	37.99768952019453	40.0	37.0	40.0	33.0	40.0
56-57	37.7552501768796	40.0	37.0	40.0	33.0	40.0
58-59	37.42225446485922	40.0	37.0	40.0	33.0	40.0
60-61	37.12674602759333	37.0	37.0	40.0	33.0	40.0
62-63	37.11031245276122	37.0	37.0	40.0	33.0	40.0
64-65	36.91959672414978	37.0	37.0	40.0	33.0	40.0
66-67	36.49264243670342	37.0	37.0	40.0	33.0	40.0
68-69	36.15837671524618	37.0	37.0	37.0	33.0	40.0
70-71	35.689899561052954	37.0	35.0	37.0	33.0	40.0
72-73	35.363853900376995	37.0	33.0	37.0	33.0	37.0
74-75	35.0912462581399	37.0	33.0	37.0	33.0	37.0
76-77	33.00645210618171	33.0	33.0	37.0	27.0	37.0
78-79	34.63472667968587	37.0	33.0	37.0	33.0	37.0
80-81	34.782353068655354	37.0	33.0	37.0	33.0	37.0
82-83	34.6616621924304	37.0	33.0	37.0	33.0	37.0
84-85	34.519350137403634	37.0	33.0	37.0	33.0	37.0
86-87	34.65188477273389	37.0	33.0	37.0	33.0	37.0
88-89	34.51091474861661	37.0	33.0	37.0	33.0	37.0
90-91	34.49137128545944	37.0	33.0	37.0	33.0	37.0
92-93	34.54667659260549	37.0	33.0	37.0	33.0	37.0
94-95	34.578826168123285	37.0	33.0	37.0	33.0	37.0
96-97	34.62309389820934	37.0	33.0	37.0	33.0	37.0
98-99	34.62065934163303	37.0	33.0	37.0	33.0	37.0
100	34.511264893996575	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	1.0
15	1.0
16	2.0
17	11.0
18	21.0
19	41.0
20	110.0
21	251.0
22	704.0
23	2105.0
24	4999.0
25	10061.0
26	17991.0
27	28935.0
28	44898.0
29	64608.0
30	92010.0
31	127495.0
32	177687.0
33	251606.0
34	369347.0
35	572383.0
36	1072956.0
37	2809683.0
38	3106543.0
39	135861.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	62.02312832475714	12.519460792766418	7.405601446338604	18.051809436137837
2	9.727761350363453	26.111122609301297	30.945746639478944	33.215369400856304
3	33.76935857474502	24.517893693482716	31.527839689747637	10.184908042024626
4	9.848654338414033	16.947776067676372	53.64950674953891	19.554062844370687
5	10.532758640277038	42.4118458848065	32.387933335515484	14.667462139400971
6	17.87644999145699	36.91275817010226	19.651371026277932	25.559420812162813
7	19.50124129515829	37.35423878872179	29.747721986328713	13.396797929791207
8	16.213491719975632	17.7022021274394	21.097651016072327	44.986655136512645
9	15.563570772319352	29.65661947522202	35.47480673344198	19.305003019016656
10-11	26.871109810319364	20.577703009748138	21.00259810904274	31.548589070889747
12-13	35.925196014472235	21.654263661027993	25.31488781950059	17.105652504999178
14-15	30.020571831449782	20.705538793239203	34.91620429069306	14.357685084617952
16-17	14.925319831295008	32.14934414000318	35.03212260717059	17.89321342153121
18-19	16.46720047620376	36.67007974523278	23.156976942991427	23.705742835572025
20-21	19.030835516706677	29.06288396648815	21.301508145788365	30.604772371016807
22-23	19.466116116203604	22.52872410843659	29.5660890897755	28.4390706855843
24-25	30.725386453243676	22.069165865950175	22.371723185184123	24.83372449562203
26-27	21.191414155500574	21.35992311116342	40.985601370339204	16.463061362996807
28-29	17.90296945190139	34.25856191859122	26.51722850078847	21.321240128718927
30-31	17.32921548370589	27.120063534659927	27.61075988860306	27.93996109303112
32-33	25.303828641900044	23.494946168427067	26.765256731447472	24.435968458225418
34-35	29.511187143234253	24.251350677825975	23.901761061940157	22.335701116999616
36-37	23.3726028942942	20.70771779200501	37.744106768543034	18.175572545157756
38-39	22.15047110331378	30.208089320590204	29.561249364231845	18.080190211864174
40-41	18.1240736883062	32.58862587662264	30.98828903654981	18.29901139852134
42-43	17.251622054532287	28.644344070898587	19.22923334445256	34.87480053011657
44-45	20.052854884500466	23.753306979596	25.33857894863075	30.855259187272782
46-47	32.347366732315905	23.54976062252915	26.20539063000109	17.897482015153855
48-49	21.71970538238904	24.068439501154444	32.53984483740492	21.67201027905159
50-51	15.56047016412792	28.312848735204092	36.82933049124401	19.297350609423976
52-53	18.030149071829193	31.97570954699541	26.739835991536793	23.2543053896386
54-55	18.234428222079238	23.147203333081723	29.261928792359313	29.356439652479725
56-57	26.106147108151145	21.617863024899105	21.864492688230822	30.411497178718932
58-59	29.16508437492555	23.363405761380356	28.5445517850686	18.926958078625496
60-61	27.481841705282473	22.72353367860094	32.929628978782794	16.864995637333795
62-63	16.79087413389031	31.964555133032015	32.78709082837501	18.457479904702673
64-65	17.902835283201913	32.39462924025322	23.391677880775163	26.310857595769704
66-67	18.824084665138873	27.788307486026458	23.273195236733365	30.114412612101304
68-69	21.8306643006889	22.355971245296228	29.511975580793692	26.301388873221182
70-71	27.608790432305252	21.52665544121295	25.605114886466602	25.259439240015197
72-73	19.620884409243537	23.126688373422535	38.18844017367956	19.063987043654365
74-75	18.627126944158263	30.169339104177638	27.252731810679037	23.950802140985058
76-77	17.472280886182705	25.127809982774462	29.204518265790757	28.195390865252072
78-79	24.36389271023012	21.330759666183425	27.5927574025015	26.71259022108495
80-81	26.493563032257754	23.087009524435985	26.102087032460286	24.317340410845972
82-83	21.32446519939524	20.863107216696957	37.343860176919996	20.468567406987813
84-85	21.157636572739605	26.551140155464008	30.596134350757293	21.695088921039098
86-87	15.959411815038422	29.14985280368158	32.847327188541456	22.043408192738536
88-89	16.43587531846918	23.459824289649063	23.244612542215425	36.85968784966633
90-91	16.88598113681983	22.089397328579487	28.469428897402736	32.55519263719795
92-93	26.77999844396874	19.48204922812594	31.158963102796378	22.57898922510894
94-95	18.258318531081382	16.444890463335575	38.865836402500825	26.430954603082213
96-97	13.753932428727698	23.071058081727656	40.77161248256917	22.403397006975474
98-99	16.891990413639306	24.248390883470766	38.04062503184152	20.81899367104841
100	28.143825880588135	0.0	71.85617411941186	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	436.0
1	752.5
2	1963.5
3	4093.5
4	9068.5
5	17011.0
6	23152.5
7	26824.5
8	27990.0
9	26198.5
10	22650.0
11	18724.5
12	16917.0
13	16778.0
14	18509.5
15	21271.0
16	22819.5
17	23076.0
18	22253.5
19	20779.5
20	18949.0
21	16927.5
22	14969.0
23	13518.5
24	12614.0
25	12126.5
26	11993.0
27	12081.0
28	12406.0
29	13129.0
30	13837.0
31	14850.5
32	16117.0
33	17585.5
34	19995.5
35	25627.0
36	32801.5
37	41144.5
38	57454.0
39	96375.5
40	166111.5
41	284370.0
42	490614.0
43	816982.5
44	1221435.5
45	1606442.5
46	1816484.5
47	1704870.0
48	1295606.5
49	776809.5
50	402890.5
51	208735.0
52	106581.0
53	64886.5
54	39498.0
55	23207.5
56	16457.5
57	11899.0
58	8470.0
59	6739.5
60	4921.0
61	3330.0
62	2484.0
63	1896.5
64	1317.0
65	877.0
66	658.0
67	527.0
68	413.0
69	304.0
70	221.5
71	157.5
72	117.0
73	88.5
74	63.5
75	43.0
76	27.0
77	14.0
78	7.5
79	5.0
80	5.5
81	4.5
82	3.5
83	3.0
84	1.5
85	0.5
86	0.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.1248199163313953E-5
2	2.699567799195349E-4
3	1.1248199163313953E-5
4	1.1248199163313953E-5
5	1.1248199163313953E-5
6	1.1248199163313953E-5
7	1.1248199163313953E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.006732047199243401
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.388478180233606E-5
26-27	5.668930062579886E-6
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.00201071246635867
36-37	0.03914116432513339
38-39	0.12497986633021067
40-41	0.16243263658992857
42-43	0.05801468904535141
44-45	0.0016432301966747185
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.010745959626238663
64-65	0.08124766061602458
66-67	0.19218681056094888
68-69	0.21239228259897638
70-71	0.08714339866187545
72-73	0.006269945897431633
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2792.0
20-21	17889.0
22-23	13100.0
24-25	22932.0
26-27	154381.0
28-29	59075.0
30-31	35943.0
32-33	45648.0
34-35	33224.0
36-37	51528.0
38-39	56135.0
40-41	62825.0
42-43	47872.0
44-45	48631.0
46-47	63774.0
48-49	83546.0
50-51	157248.0
52-53	77357.0
54-55	130554.0
56-57	109699.0
58-59	74498.0
60-61	53326.0
62-63	75016.0
64-65	145130.0
66-67	156688.0
68-69	153987.0
70-71	123383.0
72-73	194808.0
74-75	221642.0
76-77	262181.0
78-79	308580.0
80-81	237039.0
82-83	329603.0
84-85	419719.0
86-87	297505.0
88-89	260964.0
90-91	252310.0
92-93	224540.0
94-95	263403.0
96-97	414352.0
98-99	2270202.0
100-101	877283.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.00774232969862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.66952913850534	81.59297829044883
2	6.4504420527209945	11.482785682824849
3	1.2006225542583853	3.2059410883396486
4	0.34386237380499135	1.224256542580527
5	0.1394422367790302	0.6205719340552373
6	0.06667488158909446	0.35607484082071716
7	0.041008344972896835	0.2555042141900495
8	0.02581008970234805	0.18378382509864022
9	0.01595906754293296	0.12784325145152697
>10	0.04531008027251748	0.6035668812500378
>50	5.984268220484909E-4	0.0357560125813407
>100	5.257922097969362E-4	0.11991951145910523
>500	9.771056947534675E-5	0.057608756690533246
>1k	1.1725025013197788E-4	0.13340916820904647
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.1248199163313953E-5	0.0
10-11	0.0	0.0	0.0	1.1248199163313953E-5	0.0
12-13	0.0	0.0	0.0	1.1248199163313953E-5	0.0
14-15	0.0	0.0	0.0	1.687229874497093E-5	0.0
16-17	0.0	0.0	0.0	4.4992796653255813E-5	0.0
18-19	0.0	0.0	0.0	6.186509539822674E-5	0.0
20-21	0.0	0.0	0.0	1.2935429037811045E-4	0.0
22-23	0.0	0.0	0.0	2.699567799195349E-4	0.0
24-25	0.0	0.0	0.0	3.880628711343314E-4	0.0
26-27	0.0	0.0	0.0	5.849063564923255E-4	0.0
28-29	0.0	0.0	0.0	0.0014903863891390988	0.0
30-31	0.0	0.0	0.0	0.0027445605958486045	0.0
32-33	0.0	0.0	0.0	0.006822032792549913	0.0
34-35	0.0	0.0	0.0	0.011062603877119274	0.0
36-37	0.0	0.0	0.0	0.013824036771712848	0.0
38-39	0.0	0.0	0.0	0.01864389011319288	0.0
40-41	0.0	0.0	0.0	0.023615594143377647	0.0
42-43	0.0	0.0	0.0	0.030983164595348285	0.0
44-45	0.0	0.0	0.0	0.03797392037534791	0.0
46-47	0.0	0.0	0.0	0.044340401101783604	0.0
48-49	0.0	0.0	0.0	0.050003869380512184	0.0
50-51	0.0	0.0	0.0	0.05641534290360113	0.0
52-53	0.0	0.0	0.0	0.06295054661748653	0.0
54-55	0.0	0.0	0.0	0.06963197692049503	0.0
56-57	0.0	0.0	0.0	0.07732012104862011	0.0
58-59	0.0	0.0	0.0	0.08239305887127471	0.0
60-61	0.0	0.0	0.0	0.08544132084453279	0.0
62-63	0.0	0.0	0.0	0.08778657037008375	0.0
64-65	0.0	0.0	0.0	0.09236458742955253	0.0
66-67	0.0	0.0	0.0	0.10062076561542496	0.0
68-69	0.0	0.0	0.0	0.10933249586741162	0.0
70-71	0.0	0.0	0.0	0.11552462950681597	0.0
72-73	0.0	0.0	0.0	0.11969208729682378	0.0
74-75	0.0	0.0	0.0	0.12333650382573749	0.0
76-77	0.0	0.0	0.0	0.1269078070600897	0.0
78-79	0.0	0.0	0.0	0.1306703296802182	0.0
80-81	0.0	0.0	0.0	0.1317276604015697	0.0
82-83	0.0	0.0	0.0	0.13212697147186736	0.0
84-85	0.0	0.0	0.0	0.13236318365429695	0.0
86-87	0.0	0.0	0.0	0.13245316924760347	0.0
88	0.0	0.0	0.0	0.13248128974551174	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACGG	65	5.385673E-5	178.38824	94
TGCGAGT	165	0.0021905047	70.27416	94
CTGACGG	170	0.0024657862	68.207275	94
CAGACGT	175	0.0027660707	66.25849	94
TTGTGGG	180	0.0030928042	64.41798	94
AACTAAT	180	0.0030928042	64.41798	94
AGCACGT	180	0.0030928042	64.41798	94
ACAATTG	185	0.0034474717	62.676952	94
TCAGGGG	330	8.186555E-6	58.561806	94
CACCAGG	220	0.006844859	52.70562	94
GCCAGGG	305	3.9099768E-4	50.689556	94
TATACGG	235	0.008883823	49.34143	94
CTTATCG	400	2.5565212E-5	48.313488	94
CGTACAT	170	9.4807547E-7	45.471516	94
CTTATGT	600	1.1920747E-7	45.092583	94
ATATGAT	350	7.7056844E-4	44.17233	94
CTTGGAT	380	0.0011550136	40.68504	94
GAGACAG	1155	0.0	40.156666	94
AGCGGCG	385	0.0012317025	40.156662	94
CTTGGTG	690	3.5720223E-7	39.210945	94
>>END_MODULE