FastQCFastQC Report
Fri 27 May 2016
SRR2139143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8162561
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125900.051.7574231
CTTATAC102800.039.653141
GTATCAA232750.038.443661
ATCAACG276100.031.8892633
TATACAC141600.030.8669643
TCAACGC286150.030.7538094
CAACGCA291350.030.2160245
TATCAAC293700.030.1791862
AACGCAG293850.030.0002426
TTATACA139800.029.609762
ACGCAGA298200.029.590827
ACACATC158250.025.461326
CGCAGAG354950.024.8123938
GTGGTAT56100.024.3706681
TACACAT178400.023.8599055
ACATCTC170700.023.6043018
TGGTATC55150.023.1123472
GCAGAGT396300.022.1173349
ATACACA208650.021.9553554
CACATCT189600.021.096067
Produced by FastQC (version 0.11.5)