##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139143_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8162561
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86114590751603	33.0	33.0	33.0	33.0	33.0
2	32.912832626916966	33.0	33.0	33.0	33.0	33.0
3	33.586495463862384	33.0	33.0	33.0	33.0	37.0
4	36.742461342708495	37.0	37.0	37.0	37.0	37.0
5	36.75755721764285	37.0	37.0	37.0	37.0	37.0
6	36.751200879233856	37.0	37.0	37.0	37.0	37.0
7	36.74496925658504	37.0	37.0	37.0	37.0	37.0
8	36.73362232760037	37.0	37.0	37.0	37.0	37.0
9	36.72936753550754	37.0	37.0	37.0	37.0	37.0
10-11	36.71663672712523	37.0	37.0	37.0	37.0	37.0
12-13	36.956926569982144	37.0	37.0	37.0	37.0	38.5
14-15	38.939554203637805	40.0	40.0	40.0	37.0	40.0
16-17	38.88842202833155	40.0	38.5	40.0	37.0	40.0
18-19	38.93028658530086	40.0	40.0	40.0	37.0	40.0
20-21	38.964911303610975	40.0	40.0	40.0	37.0	40.0
22-23	38.99142635632158	40.0	40.0	40.0	37.0	40.0
24-25	38.971914340935164	40.0	40.0	40.0	37.0	40.0
26-27	38.87934978709814	40.0	40.0	40.0	37.0	40.0
28-29	38.800266440513546	40.0	38.5	40.0	37.0	40.0
30-31	38.73201510440151	40.0	37.0	40.0	37.0	40.0
32-33	38.683922587604854	40.0	37.0	40.0	37.0	40.0
34-35	38.65957995839118	40.0	37.0	40.0	37.0	40.0
36-37	38.62617278862821	40.0	37.0	40.0	37.0	40.0
38-39	38.554857378102476	40.0	37.0	40.0	37.0	40.0
40-41	38.52341030197737	40.0	37.0	40.0	37.0	40.0
42-43	38.46250122235206	40.0	37.0	40.0	37.0	40.0
44-45	38.37492125548602	40.0	37.0	40.0	37.0	40.0
46-47	38.289052079620724	40.0	37.0	40.0	37.0	40.0
48-49	38.243545962055336	40.0	37.0	40.0	37.0	40.0
50-51	38.16294244711605	40.0	37.0	40.0	37.0	40.0
52-53	38.09779704110876	40.0	37.0	40.0	37.0	40.0
54-55	38.02146870460978	40.0	37.0	40.0	37.0	40.0
56-57	37.90620920990868	40.0	37.0	40.0	33.0	40.0
58-59	37.77321957048916	40.0	37.0	40.0	33.0	40.0
60-61	37.59144767526536	38.5	37.0	40.0	33.0	40.0
62-63	37.396271173165886	37.0	37.0	40.0	33.0	40.0
64-65	37.177906050384536	37.0	37.0	40.0	33.0	40.0
66-67	36.94324381687295	37.0	37.0	40.0	33.0	40.0
68-69	36.68321584714343	37.0	37.0	40.0	33.0	40.0
70-71	36.4309422049146	37.0	37.0	38.5	33.0	40.0
72-73	36.181258790394104	37.0	37.0	37.0	33.0	40.0
74-75	35.72668865652338	37.0	35.0	37.0	33.0	40.0
76-77	34.485212426396814	35.0	33.0	37.0	30.0	37.0
78-79	35.60124355598017	37.0	35.0	37.0	33.0	37.0
80-81	35.71144280793554	37.0	37.0	37.0	33.0	37.0
82-83	35.623100904303506	37.0	37.0	37.0	33.0	37.0
84-85	35.515475167659616	37.0	37.0	37.0	33.0	37.0
86-87	35.426039432242376	37.0	33.0	37.0	33.0	37.0
88-89	35.3361574323433	37.0	33.0	37.0	33.0	37.0
90-91	35.27078888544834	37.0	33.0	37.0	33.0	37.0
92-93	35.19641883310839	37.0	33.0	37.0	33.0	37.0
94-95	35.15739541934817	37.0	33.0	37.0	33.0	37.0
96-97	35.12209678755295	37.0	33.0	37.0	33.0	37.0
98-99	35.08033808456963	37.0	33.0	37.0	33.0	37.0
100	34.995283270445206	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	8.0
20	35.0
21	164.0
22	562.0
23	1686.0
24	4147.0
25	8589.0
26	15352.0
27	25038.0
28	37324.0
29	53743.0
30	73806.0
31	100093.0
32	134614.0
33	183884.0
34	262228.0
35	402571.0
36	779784.0
37	2330782.0
38	3555842.0
39	192304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.567327751089486	0.0	24.28773466324486	30.144937585665655
2	15.013292463601926	24.048149346035295	38.27707619705679	22.66148199330599
3	16.55666939954846	24.15966335078098	33.20248007797779	26.081187171692765
4	12.951863657499443	23.719240507724983	36.56623380124132	26.762662033534255
5	17.311137741747867	35.656292112791434	32.46689694911492	14.565673196345784
6	28.04368972479222	37.181962521561864	19.948876332915162	14.825471420730752
7	25.939999215932257	32.62491179237886	22.4621564803199	18.97293251136898
8	24.302948547643318	31.991368885329003	21.64759320022233	22.058089366805344
9	23.755363053335834	20.077546740538907	23.449601172965203	32.71748903316006
10-11	24.703239583753188	25.846685862439493	28.21763904735291	21.232435506454408
12-13	25.188895984973342	23.986987662328037	28.079195977830977	22.744920374867643
14-15	22.665956187034926	26.063192667105334	26.2101685978212	25.06068254803854
16-17	21.794869281834465	28.71786195533485	26.999755346391897	22.487513416438787
18-19	21.811280908729557	27.62546752333413	28.88232191357111	21.680929654365197
20-21	22.682154914949095	26.847056611066648	28.823645980446116	21.64714249353814
22-23	22.508852926702243	26.90803903882913	28.603332460747577	21.97977557372105
24-25	22.29945310825206	27.07323132874998	28.871034225870375	21.756281337127586
26-27	22.23740814769061	27.379303632091883	28.73753624060394	21.64575197961357
28-29	21.908662862215998	27.228798774562023	28.784288899114784	22.0782494641072
30-31	22.390406467792854	26.970545019459053	28.821315300599842	21.81773321214825
32-33	22.08987649054431	27.309663273805977	28.856142246952903	21.744317988696814
34-35	22.26280937860151	26.734526608082003	28.902278053132303	22.100385960184184
36-37	22.024434310318643	27.305513619530576	28.779713782907372	21.89033828724341
38-39	22.011898245782657	26.99180466505602	29.017396129075745	21.97890096008558
40-41	22.22905114455831	26.71778933919495	28.70365698843897	22.349502527807772
42-43	22.290342937651687	26.75882530488976	28.751911603250562	22.198920154207986
44-45	22.117294670328018	27.275338442132437	28.427606216631705	22.17976067090784
46-47	22.463218019878664	26.82389957402866	27.94032528720795	22.772557118884727
48-49	22.355945041463997	27.024102699820478	28.105849545858096	22.514102712857422
50-51	22.279289351276788	27.37901366064631	27.75905982958813	22.582637158488776
52-53	22.45663925158783	27.34329079814736	27.891157228925163	22.30891272133964
54-55	22.312855096511175	27.4268994286128	27.806884672708403	22.45336080216762
56-57	22.62602809156686	27.88309809743767	27.041448792491995	22.449425018503476
58-59	22.286023318761945	27.7398591720055	27.7210943661991	22.253023143033452
60-61	22.561715593864047	28.009112990968582	27.240738745666114	22.188432669501253
62-63	22.250451952137443	28.320860788301967	27.233851084598133	22.19483617496246
64-65	22.472815431996313	27.919255442806048	27.139124072626142	22.4688050525715
66-67	22.43197899751356	27.819086288718953	27.1868794851018	22.56205522866568
68-69	22.521416193126313	27.70658391587772	27.211185082941835	22.56081480805413
70-71	22.55529446703053	27.304636489703437	27.32308455428507	22.81698448898096
72-73	22.561730034824805	27.374773523963448	27.318297085639177	22.745199355572566
74-75	22.500167361486174	27.580593378756095	27.234041794379642	22.68519746537809
76-77	22.737447606227335	27.34599908612612	27.06708825994648	22.84946504770006
78-79	22.491502292518494	27.44417895384882	27.22508668612789	22.839232067504792
80-81	22.578440650834747	27.484169077060756	27.15156303695713	22.78582723514737
82-83	22.766113107287293	27.30872800927432	27.21249486498393	22.712664018454458
84-85	22.74270082052	27.346785394859886	27.251551639920248	22.658962144699867
86-87	22.618873278726703	27.408056929288175	27.250805729342385	22.722264062642736
88-89	22.754548939186076	27.189603998728117	27.227238408621773	22.828608653464038
90-91	22.784721636508728	27.277797421707227	27.249064119948358	22.688416821835688
92-93	22.751217286041282	27.269270486519048	27.325436163511906	22.654076063927768
94-95	22.683822940686653	27.233066244448718	27.354258743274407	22.72885207159023
96-97	22.71934128473446	27.506169779212726	27.4831398064458	22.29134912960702
98-99	23.858863420832165	29.532242799219215	26.965841386965096	19.643052392983524
100	22.833939311236882	29.936277423363055	21.37863136282805	25.851151902572017
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1960.0
1	1731.0
2	2346.5
3	3442.0
4	4844.5
5	8092.5
6	10815.0
7	12754.5
8	14314.5
9	14525.0
10	13468.5
11	12213.0
12	13229.5
13	17132.0
14	26489.5
15	40248.5
16	52364.0
17	59598.0
18	60528.0
19	56473.5
20	50339.0
21	43736.0
22	38993.5
23	38795.0
24	43285.5
25	51980.5
26	63473.5
27	76930.0
28	91613.0
29	107523.5
30	123541.0
31	139582.0
32	157178.0
33	174616.5
34	192024.5
35	209327.0
36	227068.0
37	243879.0
38	257714.5
39	268473.0
40	279216.5
41	289977.0
42	296897.0
43	304604.0
44	312924.0
45	324776.0
46	344834.0
47	363307.0
48	367769.5
49	364106.5
50	360431.0
51	347195.5
52	324931.0
53	302209.0
54	281907.5
55	264023.0
56	245508.0
57	223397.0
58	199938.5
59	175583.0
60	149245.5
61	125478.0
62	102418.0
63	80341.5
64	62602.5
65	47613.5
66	36172.5
67	27615.0
68	20456.0
69	14539.5
70	10251.5
71	7515.5
72	5453.5
73	3731.0
74	2490.5
75	1868.0
76	1493.0
77	947.5
78	571.0
79	418.0
80	266.0
81	155.5
82	112.0
83	73.5
84	43.0
85	24.0
86	16.5
87	14.0
88	9.0
89	4.5
90	2.5
91	2.5
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.5926374087740354E-4
2	5.022933366133496E-4
3	0.0029035005067649727
4	0.012937116182041396
5	0.008575739893398653
6	1.2251056990569504E-5
7	1.2251056990569504E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	6.125528495284752E-6
20-21	5.513137070554188E-5
22-23	6.555071174595239E-4
24-25	0.014693893957880608
26-27	0.058728152097638917
28-29	0.08290459734142289
30-31	0.06739294323295997
32-33	0.03082721836553694
34-35	0.0037545998479634078
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	2.6506345685422954E-5
54-55	5.276617311592868E-4
56-57	6.742588664535244E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	5.04648060965523E-5
70-71	0.0014280288400624456
72-73	0.01731889395686845
74-75	0.02258962532272485
76-77	0.0010652496146159448
78-79	6.821049759557996E-5
80-81	0.0013635604555211176
82-83	0.018801621213928067
84-85	0.05274929481079987
86-87	0.03818799317585468
88-89	0.00869519475194555
90-91	5.6769121685180024E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	152.0
20-21	534.0
22-23	1222.0
24-25	2787.0
26-27	3417.0
28-29	8566.0
30-31	17840.0
32-33	23896.0
34-35	33389.0
36-37	46439.0
38-39	52654.0
40-41	55652.0
42-43	68279.0
44-45	80464.0
46-47	78739.0
48-49	69488.0
50-51	59393.0
52-53	57910.0
54-55	66605.0
56-57	75191.0
58-59	76010.0
60-61	78787.0
62-63	79480.0
64-65	79762.0
66-67	90590.0
68-69	75409.0
70-71	67712.0
72-73	64910.0
74-75	65970.0
76-77	66678.0
78-79	69907.0
80-81	70629.0
82-83	68217.0
84-85	69406.0
86-87	72507.0
88-89	78008.0
90-91	82126.0
92-93	85803.0
94-95	108041.0
96-97	373807.0
98-99	1373767.0
100-101	4162418.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.0731909540361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.0839344652197	74.89570633231253
2	7.5548999612941365	12.703290941690437
3	1.8855079991742458	4.755620221798168
4	0.6773909012336888	2.278016583597861
5	0.3137013324286975	1.318693601190672
6	0.17126344072255054	0.8639198373187341
7	0.09117575159916554	0.5365805462202083
8	0.055898774580515	0.37596706795234053
9	0.03583583206542864	0.2711549477010196
>10	0.12639455363354357	1.6722894265072321
>50	0.0033551619765238966	0.18854915087369734
>100	5.68085695883159E-4	0.09346648068933353
>500	7.37403759619444E-5	0.046744862147685355
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.350634194341702E-5	0.0	0.0	0.0
3	0.0	7.350634194341702E-5	0.0	0.0	0.0
4	0.0	9.800845592455603E-5	0.0	1.2251056990569504E-5	0.0
5	0.0	9.800845592455603E-5	0.0	1.2251056990569504E-5	0.0
6	3.675317097170851E-5	9.800845592455603E-5	0.0	1.2251056990569504E-5	0.0
7	3.675317097170851E-5	9.800845592455603E-5	0.0	1.2251056990569504E-5	0.0
8	3.675317097170851E-5	1.1025951291512554E-4	0.0	1.2251056990569504E-5	0.0
9	4.9004227962278015E-5	1.7151479786797305E-4	0.0	1.2251056990569504E-5	0.0
10-11	5.512975645756276E-5	1.7151479786797305E-4	0.0	1.2251056990569504E-5	0.0
12-13	6.125528495284751E-5	1.8376585485854256E-4	0.0	1.2251056990569504E-5	0.0
14-15	6.125528495284751E-5	2.4502113981139006E-4	0.0	3.675317097170851E-5	0.0
16-17	8.575739893398653E-5	2.5727219680195956E-4	0.0	9.188292742927128E-5	0.0
18-19	9.800845592455603E-5	2.5727219680195956E-4	0.0	3.307785387453766E-4	0.0
20-21	1.1025951291512554E-4	2.5727219680195956E-4	0.0	8.514484608445805E-4	0.0
22-23	1.2251056990569503E-4	2.940253677736681E-4	0.0	0.001384369439934354	0.0
24-25	1.3476162689626453E-4	3.552806527265156E-4	0.0	0.0023522029421893446	0.0
26-27	1.3476162689626453E-4	3.797827667076546E-4	0.0	0.0056109841016808324	0.0
28-29	1.4088715539154929E-4	3.797827667076546E-4	0.0	0.014750272616645682	0.0
30-31	1.4701268388683404E-4	4.2878699466993264E-4	0.0	0.016342910025419717	0.0
32-33	1.4701268388683404E-4	4.2878699466993264E-4	0.0	0.01960781671340649	0.0
34-35	1.653892693726883E-4	5.390465075850581E-4	0.0	0.024097829100450212	0.0
36-37	1.7151479786797305E-4	6.248039065190447E-4	0.0	0.0317363631340703	0.0
38-39	1.7151479786797305E-4	6.493060205001837E-4	0.0	0.04891847056334403	0.0
40-41	1.8376585485854256E-4	7.228123624436007E-4	0.0	0.0646365766822447	0.0
42-43	2.0826796883968157E-4	7.90193175891733E-4	0.0	0.07614644472488476	0.0
44-45	2.0826796883968157E-4	8.453229323492958E-4	0.0	0.09010039863714342	0.0
46-47	2.0826796883968157E-4	8.453229323492958E-4	0.0	0.10453826930052958	0.0
48-49	2.0826796883968157E-4	8.514484608445805E-4	0.0	0.1180266830471466	0.0
50-51	2.3277008282082055E-4	9.61707973759706E-4	0.0	0.13306485550307068	0.0
52-53	2.4502113981139006E-4	9.800845592455602E-4	0.0	0.16668788141368868	0.0
54-55	2.633977252972443E-4	9.86210087740845E-4	0.0	0.20639355712012444	0.0
56-57	2.8177431078309857E-4	0.0010168377302172688	0.0	0.24431057850593704	0.0
58-59	2.940253677736681E-4	0.0010168377302172688	0.0	0.26820626516604285	0.0
60-61	2.940253677736681E-4	0.0010658419581795469	0.0	0.2832628142074528	0.0
62-63	3.1240195325952234E-4	0.001084218543665401	0.0	0.293872229561286	0.0
64-65	3.185274817548071E-4	0.00123735675604752	0.0	0.2992381925231554	0.0
66-67	3.430295957359461E-4	0.0012496078130380893	0.0	0.3049900637802278	1.2251056990569504E-5
68-69	3.6140618122180036E-4	0.0012986120410003674	0.0	0.3105826712964228	1.2251056990569504E-5
70-71	3.797827667076546E-4	0.0013231141549815064	0.0	0.3151829431963816	1.2251056990569504E-5
72-73	4.0428488068879363E-4	0.0013231141549815064	0.0	0.31896851980646757	1.2251056990569504E-5
74-75	4.1653593767936313E-4	0.0013598673259532148	0.0	0.3219945308831383	1.2251056990569504E-5
76-77	4.1653593767936313E-4	0.0014272481394013471	0.0	0.3248551526904363	1.2251056990569504E-5
78-79	4.1653593767936313E-4	0.001457875781877771	0.0	0.3265335474981443	1.2251056990569504E-5
80-81	4.2878699466993264E-4	0.001457875781877771	0.0	0.3271583514046633	1.2251056990569504E-5
82-83	4.4103805166050214E-4	0.0014640013103730557	0.0	0.32750138100039927	1.2251056990569504E-5
84-85	4.4103805166050214E-4	0.00148237789585891	0.0	0.32774640214021067	1.2251056990569504E-5
86-87	4.4103805166050214E-4	0.0015191310668306185	0.0	0.3278382850676399	1.2251056990569504E-5
88	4.4103805166050214E-4	0.0015681352947928965	0.0	0.3278382850676399	1.2251056990569504E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12590	0.0	51.757423	1
CTTATAC	10280	0.0	39.65314	1
GTATCAA	23275	0.0	38.44366	1
ATCAACG	27610	0.0	31.889263	3
TATACAC	14160	0.0	30.866964	3
TCAACGC	28615	0.0	30.753809	4
CAACGCA	29135	0.0	30.216024	5
TATCAAC	29370	0.0	30.179186	2
AACGCAG	29385	0.0	30.000242	6
TTATACA	13980	0.0	29.60976	2
ACGCAGA	29820	0.0	29.59082	7
ACACATC	15825	0.0	25.46132	6
CGCAGAG	35495	0.0	24.812393	8
GTGGTAT	5610	0.0	24.370668	1
TACACAT	17840	0.0	23.859905	5
ACATCTC	17070	0.0	23.604301	8
TGGTATC	5515	0.0	23.112347	2
GCAGAGT	39630	0.0	22.117334	9
ATACACA	20865	0.0	21.955355	4
CACATCT	18960	0.0	21.09606	7
>>END_MODULE