FastQCFastQC Report
Fri 27 May 2016
SRR2139142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8040239
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129750.049.460251
GTATCAA232950.036.7678831
ATCAACG275000.030.591933
TCAACGC283300.029.6798154
AACGCAG288800.029.182876
CAACGCA291200.028.9296385
ACGCAGA294800.028.588927
TATCAAC296650.028.5874732
CGCAGAG340650.024.716168
GTGGTAT59850.022.8861521
GCAGAGT386150.021.7600779
GTACATG252800.021.121181
TACATGG248450.020.7591742
ACATGGG244800.020.4468693
TGGTATC62900.020.432062
GAGTACT247300.020.21050312-13
CAGAGTA378100.019.33365410-11
AGAGTAC354050.019.05321910-11
CGAGACC74000.018.67324686-87
AGACCGA77000.018.35412488-89
Produced by FastQC (version 0.11.5)