##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139142_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8040239
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86510251747492	33.0	33.0	33.0	33.0	33.0
2	32.91456634560241	33.0	33.0	33.0	33.0	33.0
3	33.58586069394206	33.0	33.0	33.0	33.0	37.0
4	36.74177546214733	37.0	37.0	37.0	37.0	37.0
5	36.756717555286606	37.0	37.0	37.0	37.0	37.0
6	36.75231420359519	37.0	37.0	37.0	37.0	37.0
7	36.74512673068549	37.0	37.0	37.0	37.0	37.0
8	36.73391835740206	37.0	37.0	37.0	37.0	37.0
9	36.72950070265324	37.0	37.0	37.0	37.0	37.0
10-11	36.718233749021636	37.0	37.0	37.0	37.0	37.0
12-13	36.95902298426701	37.0	37.0	37.0	37.0	38.5
14-15	38.935380216931364	40.0	40.0	40.0	37.0	40.0
16-17	38.886808775211776	40.0	38.5	40.0	37.0	40.0
18-19	38.931041913057555	40.0	40.0	40.0	37.0	40.0
20-21	38.96620601328681	40.0	40.0	40.0	37.0	40.0
22-23	38.99572371528002	40.0	40.0	40.0	37.0	40.0
24-25	38.976484318190266	40.0	40.0	40.0	37.0	40.0
26-27	38.89273343977102	40.0	40.0	40.0	37.0	40.0
28-29	38.82272520330574	40.0	38.5	40.0	37.0	40.0
30-31	38.75283962171009	40.0	37.0	40.0	37.0	40.0
32-33	38.71234591569376	40.0	37.0	40.0	37.0	40.0
34-35	38.68384713570754	40.0	37.0	40.0	37.0	40.0
36-37	38.647852189890344	40.0	37.0	40.0	37.0	40.0
38-39	38.59907662402194	40.0	37.0	40.0	37.0	40.0
40-41	38.5506992196308	40.0	37.0	40.0	37.0	40.0
42-43	38.48196687171628	40.0	37.0	40.0	37.0	40.0
44-45	38.392439867222116	40.0	37.0	40.0	37.0	40.0
46-47	38.307660239519215	40.0	37.0	40.0	37.0	40.0
48-49	38.25624609392748	40.0	37.0	40.0	37.0	40.0
50-51	38.20800342997599	40.0	37.0	40.0	37.0	40.0
52-53	38.14678146323941	40.0	37.0	40.0	37.0	40.0
54-55	38.07197827296812	40.0	37.0	40.0	37.0	40.0
56-57	37.968666959970705	40.0	37.0	40.0	33.0	40.0
58-59	37.83703585136662	40.0	37.0	40.0	33.0	40.0
60-61	37.665678831160214	40.0	37.0	40.0	33.0	40.0
62-63	37.46956423415392	37.0	37.0	40.0	33.0	40.0
64-65	37.24629618902152	37.0	37.0	40.0	33.0	40.0
66-67	37.00850856678801	37.0	37.0	40.0	33.0	40.0
68-69	36.744256837221485	37.0	37.0	40.0	33.0	40.0
70-71	36.48616940131509	37.0	37.0	40.0	33.0	40.0
72-73	36.231639700015165	37.0	37.0	37.0	33.0	40.0
74-75	35.767733981476724	37.0	35.0	37.0	33.0	40.0
76-77	34.52694518701939	35.0	33.0	37.0	30.0	37.0
78-79	35.63977971112943	37.0	35.0	37.0	33.0	37.0
80-81	35.73837754006386	37.0	37.0	37.0	33.0	37.0
82-83	35.64440760810655	37.0	37.0	37.0	33.0	37.0
84-85	35.53881430295917	37.0	37.0	37.0	33.0	37.0
86-87	35.44242895882968	37.0	33.0	37.0	33.0	37.0
88-89	35.34663024797416	37.0	33.0	37.0	33.0	37.0
90-91	35.27671431187065	37.0	33.0	37.0	33.0	37.0
92-93	35.20125534453089	37.0	33.0	37.0	33.0	37.0
94-95	35.156550629653125	37.0	33.0	37.0	33.0	37.0
96-97	35.11919616815146	37.0	33.0	37.0	33.0	37.0
98-99	35.07851952556267	37.0	33.0	37.0	33.0	37.0
100	34.99068557608208	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	7.0
20	46.0
21	154.0
22	559.0
23	1471.0
24	3671.0
25	7745.0
26	14024.0
27	23231.0
28	35685.0
29	51191.0
30	71312.0
31	97364.0
32	132063.0
33	180770.0
34	258831.0
35	396289.0
36	763004.0
37	2261341.0
38	3557901.0
39	183574.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.008090569604704	0.0	24.39485213411316	29.59705729628213
2	15.010087444418312	23.884252529758925	38.50923727825937	22.5964227475634
3	16.582135367822467	24.589416444237703	33.13837527767344	25.690072910266395
4	12.915538398597182	23.250857450562172	37.35684434807822	26.476759802762423
5	17.268834578119257	36.313281800502146	32.33468104809544	14.083202573283158
6	27.922839109633436	37.19788677923629	20.394816621744702	14.484457489385576
7	26.01244813742477	33.003484100410446	22.453586267771396	18.53048149439339
8	24.12061382752428	32.0760862954447	22.162152144979768	21.641147732051248
9	23.860310620119627	20.274173441859126	23.7072679058421	32.158248032179145
10-11	24.64051752690436	25.944577020658215	28.68686490538403	20.72804054705339
12-13	25.029933811669032	24.22056732393154	28.539512320467093	22.209986543932338
14-15	22.4833117522999	26.481694138694134	26.790155118523217	24.244838990482744
16-17	21.410259073144466	28.792004814782246	27.668823028768173	22.12891308330511
18-19	21.47475342461835	27.746693848279886	29.60328666846844	21.175266058633333
20-21	22.32965222517295	27.013722796927443	29.510664500584603	21.145960477315
22-23	22.175223887874704	26.85166181300542	29.285060176940952	21.68805412217892
24-25	21.80083590519937	27.050404177634586	29.543890622546293	21.60486929461975
26-27	21.778858137087536	27.300988182568602	29.35861065278032	21.561543027563545
28-29	21.70021847189568	27.162022095454503	29.37542487483777	21.76233455781205
30-31	21.95821162303148	27.137101043576994	29.344030633404362	21.560656699987167
32-33	21.795626859852497	27.354883676612808	29.273593727062497	21.5758957364722
34-35	21.86027810286258	27.22666994249307	29.24852078315578	21.664531171488573
36-37	21.902445867388227	27.317083402964883	29.16097514700105	21.619495582645843
38-39	21.73390157421027	27.50261506408745	29.159675838531772	21.603807523170506
40-41	22.034510535312933	27.256459796358513	28.867616438817763	21.8414132295108
42-43	21.910993306764773	27.336462989941694	28.862530652198544	21.890013051094993
44-45	22.024771499660844	27.643565492355766	28.500838818221837	21.830824189761557
46-47	22.13003184581586	27.477766657308162	28.156838888791608	22.235362608084376
48-49	22.0032887929584	27.691043179583414	28.159170213053475	22.14649781440471
50-51	22.078854799920585	27.9459616197213	27.83190699900554	22.14327658135258
52-53	22.25200173149665	27.740370604133247	27.811366816164394	22.196260848205707
54-55	22.185693683800935	27.657405348250503	27.910749532209334	22.246151435739232
56-57	22.163686090862267	27.99607905205011	27.657326017005285	22.182908840082334
58-59	22.171596359128216	27.719364675207096	27.77445275134374	22.334586214320943
60-61	22.343658588214506	27.781598996828137	27.836171745955728	22.038570669001622
62-63	22.04810500479158	27.97097371551508	27.769548680519907	22.211372599173433
64-65	22.18399680920578	27.72887159599114	27.67288418944492	22.41424740535816
66-67	22.091457714552803	27.851423533297297	27.801775859645435	22.255342892504466
68-69	22.17287293347949	28.001858957381813	27.596936761531705	22.22833134760699
70-71	22.212953667814343	27.646947632894385	27.50520508748141	22.634893611809854
72-73	22.19903544205249	27.69149224490433	27.52016956593862	22.58930274710456
74-75	22.114418043313137	27.861625839653726	27.627945888618775	22.396010228414365
76-77	22.33706327558439	27.50147973461774	27.486434639874187	22.67502234992368
78-79	22.181444293664804	27.548828710196894	27.45468709347335	22.815039902664957
80-81	22.429286556824394	27.84516833642381	27.319529135232845	22.40601597151895
82-83	22.477877889905944	27.47196095950013	27.42124552063367	22.62891562996025
84-85	22.354698667511048	27.504559587360284	27.477370573947614	22.66337117118106
86-87	22.299835717983253	27.716308815327217	27.434795696576593	22.54905977011294
88-89	22.607590096472457	27.56593527058502	27.4025946437325	22.42387998921003
90-91	22.533076002234882	27.616217770380146	27.451175743775135	22.39953048360984
92-93	22.4250187743681	27.38104046004917	27.488168547532588	22.70577221805014
94-95	22.591209388026044	27.526695568158555	27.54215673311181	22.339938310703594
96-97	22.767097523830934	27.59853696588857	27.669136052129552	21.965229458150944
98-99	23.554579860081617	29.751086361700636	27.30018892130321	19.39414485691454
100	22.68325922022876	30.022103172027958	21.648662235054932	25.645975372688344
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1850.0
1	1692.0
2	2430.5
3	3557.5
4	5011.5
5	8347.0
6	11116.0
7	13109.5
8	14715.5
9	14875.0
10	13760.0
11	12546.5
12	13581.5
13	17309.0
14	26362.0
15	40026.0
16	52178.0
17	59373.0
18	60465.0
19	56743.0
20	51154.0
21	45428.0
22	41640.0
23	42290.5
24	47788.5
25	57457.5
26	70121.5
27	84340.0
28	99990.5
29	117814.0
30	135268.5
31	151913.5
32	169850.0
33	187541.0
34	204730.0
35	219445.0
36	234997.0
37	252729.0
38	266474.5
39	277616.5
40	288565.0
41	296813.0
42	302739.5
43	310047.5
44	316187.5
45	320299.5
46	322029.5
47	323058.0
48	325841.5
49	326056.5
50	323316.5
51	315947.0
52	302241.5
53	287454.0
54	272371.0
55	253176.0
56	233941.0
57	212669.0
58	189179.0
59	165809.0
60	139591.0
61	114053.5
62	90508.0
63	70139.5
64	53549.5
65	40380.5
66	30776.5
67	23832.5
68	18434.0
69	13436.5
70	9411.5
71	6735.5
72	4809.0
73	3389.5
74	2309.0
75	1536.0
76	1030.5
77	657.0
78	405.5
79	260.5
80	183.5
81	130.5
82	89.0
83	59.5
84	40.5
85	27.5
86	21.5
87	17.5
88	14.0
89	9.5
90	6.0
91	4.5
92	4.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.7412417715443533E-4
2	5.721222963645732E-4
3	0.002723799628344381
4	0.012437441225316809
5	0.008755958622623034
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.218893096730155E-6
22-23	4.913343852035459E-4
24-25	0.01421468883113018
26-27	0.055025440086835894
28-29	0.0787794539547728
30-31	0.0640052935152443
32-33	0.028970229292333238
34-35	0.003900238422452632
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.3470487139288138E-5
54-55	4.208808493104003E-4
56-57	5.955730847242418E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	2.1649343703753845E-5
70-71	0.0010497177680696703
72-73	0.016180597255437964
74-75	0.022441740120189308
76-77	8.858809643249729E-4
78-79	7.5853363615007465E-6
80-81	0.0010504239534077939
82-83	0.018051809079602667
84-85	0.04886773811154003
86-87	0.03563601050941515
88-89	0.0086729107883716
90-91	5.683821359768191E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	148.0
20-21	500.0
22-23	1204.0
24-25	3063.0
26-27	3612.0
28-29	8719.0
30-31	17960.0
32-33	23747.0
34-35	32956.0
36-37	46215.0
38-39	53636.0
40-41	56384.0
42-43	67362.0
44-45	80423.0
46-47	78516.0
48-49	68566.0
50-51	59489.0
52-53	57269.0
54-55	60719.0
56-57	59188.0
58-59	44345.0
60-61	65067.0
62-63	64293.0
64-65	67897.0
66-67	72625.0
68-69	70224.0
70-71	67802.0
72-73	65491.0
74-75	66391.0
76-77	67199.0
78-79	70118.0
80-81	70790.0
82-83	68861.0
84-85	69123.0
86-87	73359.0
88-89	78408.0
90-91	82009.0
92-93	86971.0
94-95	109784.0
96-97	398989.0
98-99	1371093.0
100-101	4129724.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.93641305308131
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.69699192459922	74.44907350748977
2	7.851063459509813	13.179802108867383
3	1.9585821971335067	4.931890928910286
4	0.7315249253845765	2.456063131828172
5	0.30742731892519887	1.2902173212553425
6	0.15126553197698614	0.7618011703628633
7	0.09419599134481668	0.5534531546224103
8	0.05598131702125569	0.3759096759001287
9	0.03374705793278861	0.25493462945755274
>10	0.11621233852523966	1.5185440890472435
>50	0.002547071399416304	0.13894743136715226
>100	4.1237077044063876E-4	0.0622531250714895
>500	4.849547671678577E-5	0.02710972582024344
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.731232367595043E-5	0.0	0.0	1.243744122531681E-5
3	0.0	3.731232367595043E-5	0.0	1.243744122531681E-5	1.243744122531681E-5
4	0.0	3.731232367595043E-5	0.0	1.243744122531681E-5	2.487488245063362E-5
5	0.0	3.731232367595043E-5	0.0	2.487488245063362E-5	2.487488245063362E-5
6	0.0	3.731232367595043E-5	0.0	2.487488245063362E-5	2.487488245063362E-5
7	0.0	3.731232367595043E-5	0.0	3.731232367595043E-5	2.487488245063362E-5
8	0.0	4.974976490126724E-5	0.0	4.974976490126724E-5	2.487488245063362E-5
9	0.0	1.2437441225316808E-4	0.0	9.949952980253448E-5	2.487488245063362E-5
10-11	0.0	1.2437441225316808E-4	0.0	1.368118534784849E-4	2.487488245063362E-5
12-13	0.0	1.2437441225316808E-4	0.0	1.6168673592911853E-4	2.487488245063362E-5
14-15	0.0	1.8656161837975215E-4	0.0	1.9899905960506895E-4	4.353104428860883E-5
16-17	0.0	2.1143650083038578E-4	0.0	2.4874882450633617E-4	4.974976490126724E-5
18-19	0.0	2.1143650083038578E-4	0.0	4.0421683982279626E-4	4.974976490126724E-5
20-21	0.0	2.1143650083038578E-4	0.0	9.203706506734439E-4	4.974976490126724E-5
22-23	0.0	2.2387394205570257E-4	0.0	0.0017163668890937196	5.596848551392564E-5
24-25	0.0	2.3009266266836099E-4	0.0	0.0027113621871190643	6.218720612658404E-5
26-27	0.0	2.3631138328101937E-4	0.0	0.006187627009595113	6.218720612658404E-5
28-29	0.0	2.3631138328101937E-4	0.0	0.01540377095755487	6.218720612658404E-5
30-31	0.0	3.1093603063292024E-4	0.0	0.017039294478684026	6.218720612658404E-5
32-33	0.0	3.1093603063292024E-4	0.0	0.01976931282764107	6.218720612658404E-5
34-35	0.0	3.669045161468459E-4	0.0	0.02335129590053231	6.218720612658404E-5
36-37	0.0	4.2287300166077155E-4	0.0	0.02928395536500843	6.218720612658404E-5
38-39	0.0	4.353104428860883E-4	0.0	0.04400366705517088	6.218720612658404E-5
40-41	0.0	4.974976490126723E-4	0.0	0.05797713227181431	6.218720612658404E-5
42-43	0.0	5.969971788152068E-4	0.0	0.06815095919412345	6.218720612658404E-5
44-45	0.0	6.964967086177413E-4	0.0	0.08035830775677191	6.218720612658404E-5
46-47	0.0	6.964967086177413E-4	0.0	0.0929325608355672	6.840592673924245E-5
48-49	0.0	6.964967086177413E-4	0.0	0.10570581297396756	8.706208857721766E-5
50-51	0.0	7.897775178076174E-4	0.0	0.11987827725021608	8.706208857721766E-5
52-53	0.0	8.208711208709095E-4	0.0	0.14941098143973086	9.328080918987608E-5
54-55	0.0	8.333085620962263E-4	0.0	0.18432909767980776	9.949952980253448E-5
56-57	0.0	8.333085620962263E-4	0.0	0.2257830892837887	1.0571825041519287E-4
58-59	0.0	8.519647239342014E-4	0.0	0.44088241655503024	1.1193697102785129E-4
60-61	0.0	8.830583269974935E-4	0.0	0.5801568833961279	1.1193697102785129E-4
62-63	0.0	9.141519300607855E-4	0.0	0.5864937597004267	1.368118534784849E-4
64-65	0.0	0.0010198701804759783	0.0	0.5924139817236775	1.368118534784849E-4
66-67	0.0	0.0010198701804759783	0.0	0.5982409229377386	1.4303057409114332E-4
68-69	0.0	0.0010323076217012951	0.0	0.6043477065793691	1.492492947038017E-4
70-71	0.0	0.001044745062926612	0.0	0.6094221825992984	1.6168673592911853E-4
72-73	0.0	0.001044745062926612	0.0	0.6133959450707871	1.6168673592911853E-4
74-75	1.243744122531681E-5	0.0010571825041519288	0.0	0.6164804304946656	1.6168673592911853E-4
76-77	1.243744122531681E-5	0.0010882761072152209	0.0	0.6194529789475164	1.6168673592911853E-4
78-79	1.243744122531681E-5	0.0011131509896658545	0.0	0.621188001998448	1.6168673592911853E-4
80-81	1.243744122531681E-5	0.0011255884308911713	0.0	0.6217476868535874	1.6168673592911853E-4
82-83	1.243744122531681E-5	0.0011442445927291466	0.0	0.6220026543987063	1.6168673592911853E-4
84-85	1.243744122531681E-5	0.0011566820339544634	0.0	0.6222514032232127	1.6168673592911853E-4
86-87	1.243744122531681E-5	0.001200213078243072	0.0	0.6223509027530152	1.6168673592911853E-4
88	1.243744122531681E-5	0.0012934938874329483	0.0	0.6223571214736279	1.6168673592911853E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12975	0.0	49.46025	1
GTATCAA	23295	0.0	36.767883	1
ATCAACG	27500	0.0	30.59193	3
TCAACGC	28330	0.0	29.679815	4
AACGCAG	28880	0.0	29.18287	6
CAACGCA	29120	0.0	28.929638	5
ACGCAGA	29480	0.0	28.58892	7
TATCAAC	29665	0.0	28.587473	2
CGCAGAG	34065	0.0	24.71616	8
GTGGTAT	5985	0.0	22.886152	1
GCAGAGT	38615	0.0	21.760077	9
GTACATG	25280	0.0	21.12118	1
TACATGG	24845	0.0	20.759174	2
ACATGGG	24480	0.0	20.446869	3
TGGTATC	6290	0.0	20.43206	2
GAGTACT	24730	0.0	20.210503	12-13
CAGAGTA	37810	0.0	19.333654	10-11
AGAGTAC	35405	0.0	19.053219	10-11
CGAGACC	7400	0.0	18.673246	86-87
AGACCGA	7700	0.0	18.354124	88-89
>>END_MODULE