FastQCFastQC Report
Fri 27 May 2016
SRR2139141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7850449
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126100.048.559931
GTATCAA225050.036.424161
TCAACGC273950.029.2838254
ATCAACG274400.029.2513483
TATCAAC279550.028.95672
CAACGCA278750.028.7602235
AACGCAG282400.028.3402866
ACGCAGA323650.024.7282457
CGCAGAG330900.024.1993458
GCAGAGT378100.021.0994459
GTGGTAT63000.020.9972481
GTACATG258600.020.9728891
GAGTACT221450.020.51681712-13
TACATGG257450.020.469882
TGGTATC62600.019.7680992
ACATGGG264650.019.5905043
CAGAGTA364200.019.13441810-11
AGAGTAC338750.018.8529210-11
GTACTTT245500.018.46377414-15
CATGGGG195700.018.2069384
Produced by FastQC (version 0.11.5)