FastQCFastQC Report
Fri 27 May 2016
SRR2139140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6175251
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95100.050.725861
CTTATAC77100.037.804071
GTATCAA174000.037.265161
TATACAC102900.030.5833263
TCAACGC212350.029.9383624
ATCAACG213850.029.7086143
TATCAAC218750.029.2748722
CAACGCA217800.029.1667525
TTATACA103550.028.963572
AACGCAG221600.028.7391196
ACGCAGA252400.025.2321247
CGCAGAG265550.024.0144428
GTGGTAT45400.024.0053311
TACACAT132550.022.8793055
TGGTATC44200.022.650092
ACACATC129700.022.5324276
ACATCTC132600.021.9759378
GCAGAGT292550.021.7114879
GAGTACT182550.020.23111512-13
CACATCT143600.020.0866787
Produced by FastQC (version 0.11.5)