##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139140_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6175251
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.804669964022516	33.0	33.0	33.0	33.0	33.0
2	32.800176867304664	33.0	33.0	33.0	33.0	33.0
3	32.80113941927219	33.0	33.0	33.0	33.0	33.0
4	36.743698515250635	37.0	37.0	37.0	37.0	37.0
5	36.75835233256106	37.0	37.0	37.0	37.0	37.0
6	36.76049022136914	37.0	37.0	37.0	37.0	37.0
7	36.746960083080026	37.0	37.0	37.0	37.0	37.0
8	36.748050727006884	37.0	37.0	37.0	37.0	37.0
9	36.7361383367251	37.0	37.0	37.0	37.0	37.0
10-11	36.71088762221973	37.0	37.0	37.0	37.0	37.0
12-13	36.694535331438345	37.0	37.0	37.0	37.0	37.0
14-15	38.950703704189515	40.0	40.0	40.0	37.0	40.0
16-17	38.90173581608262	40.0	40.0	40.0	37.0	40.0
18-19	38.94463472011097	40.0	40.0	40.0	37.0	40.0
20-21	38.97541878301682	40.0	40.0	40.0	37.0	40.0
22-23	39.00356275835422	40.0	40.0	40.0	37.0	40.0
24-25	38.993501835771085	40.0	40.0	40.0	37.0	40.0
26-27	38.90412591465953	40.0	40.0	40.0	37.0	40.0
28-29	38.827538515452076	40.0	38.5	40.0	37.0	40.0
30-31	38.75076110910344	40.0	37.0	40.0	37.0	40.0
32-33	38.713500565800175	40.0	37.0	40.0	37.0	40.0
34-35	38.68646096505074	40.0	37.0	40.0	37.0	40.0
36-37	38.64422864075975	40.0	37.0	40.0	37.0	40.0
38-39	38.59425403446028	40.0	37.0	40.0	37.0	40.0
40-41	38.55322740918008	40.0	37.0	40.0	37.0	40.0
42-43	38.49806237054305	40.0	37.0	40.0	37.0	40.0
44-45	38.410350066446114	40.0	37.0	40.0	37.0	40.0
46-47	38.321369891563634	40.0	37.0	40.0	37.0	40.0
48-49	38.2747176314782	40.0	37.0	40.0	37.0	40.0
50-51	38.205725031903825	40.0	37.0	40.0	37.0	40.0
52-53	38.141176463299445	40.0	37.0	40.0	37.0	40.0
54-55	38.07065658581988	40.0	37.0	40.0	37.0	40.0
56-57	37.96049452103131	40.0	37.0	40.0	33.0	40.0
58-59	37.824970352409395	40.0	37.0	40.0	33.0	40.0
60-61	37.654689912490596	40.0	37.0	40.0	33.0	40.0
62-63	37.4677040343924	37.0	37.0	40.0	33.0	40.0
64-65	37.24776786589013	37.0	37.0	40.0	33.0	40.0
66-67	37.018922886925665	37.0	37.0	40.0	33.0	40.0
68-69	36.76624170965986	37.0	37.0	40.0	33.0	40.0
70-71	36.51622372997064	37.0	37.0	40.0	33.0	40.0
72-73	36.280105757203685	37.0	37.0	37.0	33.0	40.0
74-75	36.02180731142485	37.0	37.0	37.0	33.0	40.0
76-77	34.37198187658553	35.0	33.0	37.0	30.0	37.0
78-79	35.63994952554903	37.0	35.0	37.0	33.0	37.0
80-81	35.771842784170914	37.0	37.0	37.0	33.0	37.0
82-83	35.685083795723386	37.0	37.0	37.0	33.0	37.0
84-85	35.57805994563945	37.0	37.0	37.0	33.0	37.0
86-87	35.48939603874976	37.0	35.0	37.0	33.0	37.0
88-89	35.3999540312547	37.0	33.0	37.0	33.0	37.0
90-91	35.34021288126617	37.0	33.0	37.0	33.0	37.0
92-93	35.26797158756641	37.0	33.0	37.0	33.0	37.0
94-95	35.22789484249185	37.0	33.0	37.0	33.0	37.0
96-97	35.19725080478736	37.0	33.0	37.0	33.0	37.0
98-99	35.16058977876352	37.0	33.0	37.0	33.0	37.0
100	35.087612996159336	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	3.0
19	2.0
20	21.0
21	91.0
22	401.0
23	1252.0
24	3129.0
25	6295.0
26	11260.0
27	18418.0
28	27642.0
29	39382.0
30	54405.0
31	73694.0
32	99251.0
33	136473.0
34	196392.0
35	301649.0
36	587101.0
37	1767572.0
38	2734342.0
39	116475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.65257412208832	20.03889396560561	14.423122234221736	23.88540967808434
2	14.58729369866909	20.748921784717737	41.820081483327556	22.84370303328561
3	17.95577341465804	26.110899243673735	30.822841846760735	25.110485494907493
4	12.34207167752886	18.343250754738982	38.16533642571166	31.149341142020496
5	13.298189356395557	37.46754445632106	35.461795096038614	13.772471091244775
6	29.83120848043262	37.60035017200111	18.55488950975434	14.013551837811935
7	26.19360735296428	32.86423499222946	22.569884203897136	18.37227345090912
8	24.576992902798604	36.30835410576833	20.952848718214046	18.161804273219015
9	25.278114201349872	16.803997116878325	20.93317340461141	36.9847152771604
10-11	24.114315353335435	26.67221947739452	28.52812784452	20.685337324750037
12-13	25.18903280206748	24.29320686721884	28.498339581662346	22.01942074905133
14-15	22.380458705241292	25.637743307923838	26.2536373015445	25.728160685290362
16-17	21.82300768017365	28.942710182954507	26.800894408988395	22.43338772788345
18-19	21.895271949269755	27.626626027023033	28.821686762206106	21.65641526150111
20-21	22.595897855779782	26.751417626109454	28.958073958820503	21.69461055929026
22-23	22.406073475035658	27.08851620359386	28.682687603235653	21.82272271813483
24-25	22.434608574868133	27.10473498443877	28.842811904587172	21.617844536105924
26-27	22.278366948833234	27.320675721158988	28.856873586654192	21.544083743353585
28-29	22.140568905320702	27.104137687965352	28.877066263891116	21.878227142822833
30-31	22.22952976261838	27.133455235778236	29.020644464956458	21.616370536646922
32-33	22.287192738232594	27.284475483592384	28.797178221794056	21.63115355638096
34-35	22.518096083366228	27.09497035743993	28.90253449213333	21.484399067060515
36-37	22.117475808197717	27.330799160877717	28.612609420178565	21.939115610745997
38-39	21.99144770988163	26.99817782405945	28.85697204875755	22.153402417301375
40-41	22.127450320610297	26.844259149278443	28.9254524321498	22.10283809796146
42-43	22.239814708328765	26.894631074868215	28.77573904043092	22.089815176372102
44-45	22.05944215651085	27.228503169672614	28.498067363301967	22.213987310514568
46-47	22.510514010130468	26.98793059637023	27.877307547642182	22.62424784585712
48-49	22.406242109714533	27.192658159830955	27.8685356026031	22.53256412785141
50-51	22.275301988903923	27.423846427390696	27.877298602294225	22.423552981411152
52-53	22.415070317632562	27.503252687562902	27.94557970018493	22.13609729461961
54-55	22.330264649710063	27.50241995282856	27.636866676120707	22.53044872134067
56-57	22.625570296538175	27.76849566359173	27.238735541596075	22.367198498274018
58-59	22.168154756016538	27.98387064863813	27.721744857755297	22.126229737590034
60-61	22.54016216584524	28.175557019237317	27.192568534375074	22.09171228054237
62-63	22.246314130782647	28.415560512666183	27.240536785431065	22.097588571120102
64-65	22.385703363736926	28.107291616861808	27.234408777847975	22.272596241553288
66-67	22.46488742398008	27.971599807229495	27.117712556923312	22.445800211867116
68-69	22.489463941151765	27.754973004559098	27.2297488541394	22.52581420014974
70-71	22.484972416595326	27.546357072372658	27.29297262263957	22.675697888392442
72-73	22.556316765611033	27.565590070430527	27.199859857120256	22.678233306838177
74-75	22.45389914016521	27.682776742084737	27.191010069013505	22.672314048736546
76-77	22.570926189054152	27.66653700911984	27.08427473514405	22.678262066681956
78-79	22.48922956023526	27.623274017925866	27.129372922394307	22.758123499444576
80-81	22.499515242188636	27.647638151938693	27.165817654955195	22.687028950917476
82-83	22.65548430541314	27.618544762604387	27.215051295001096	22.510919636981374
84-85	22.6951901346269	27.59052815787105	27.1215267648803	22.592754942621752
86-87	22.508548493070872	27.695033694889077	27.197648828383937	22.59876898365612
88-89	22.656732410258723	27.558607892306885	27.164226396046242	22.62043330138815
90-91	22.659465645697658	27.68733506365919	27.121322791052275	22.53187649959087
92-93	22.7348574065092	27.57689464791725	27.233085951850157	22.455161993723397
94-95	22.482306913868207	27.734415132762564	27.254688860667336	22.528589092701893
96-97	22.594771989891107	27.824453763915635	27.305212285312226	22.275561960881028
98-99	23.748441974477643	29.69709775548994	27.44229437894439	19.11216589108803
100	22.531831425127784	30.72487558833285	21.388044909712963	25.3552480768264
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2564.0
1	2138.0
2	2244.5
3	2917.5
4	3802.0
5	5955.0
6	7848.0
7	9298.0
8	10468.0
9	10683.0
10	9969.5
11	9145.5
12	9982.0
13	13073.0
14	20297.5
15	30144.5
16	38269.0
17	43278.0
18	44422.5
19	41978.0
20	37655.5
21	33027.5
22	30055.5
23	30498.0
24	34406.5
25	41259.0
26	50135.5
27	60542.0
28	72323.0
29	85276.0
30	97483.0
31	109271.5
32	122546.0
33	136029.5
34	148976.0
35	160083.5
36	172535.5
37	184815.0
38	194699.5
39	203427.5
40	211344.0
41	218357.0
42	223356.5
43	229549.5
44	236470.5
45	246251.5
46	261265.5
47	275595.0
48	276152.0
49	270115.5
50	267008.0
51	258559.0
52	242151.5
53	224068.5
54	210353.5
55	197729.5
56	183621.0
57	167961.0
58	151533.5
59	132448.0
60	110368.0
61	91512.5
62	74555.5
63	59000.5
64	46142.0
65	34533.0
66	26478.0
67	20260.5
68	14445.0
69	10020.0
70	7139.0
71	5114.5
72	3553.5
73	2478.0
74	1850.0
75	1386.0
76	909.0
77	521.5
78	347.5
79	258.5
80	166.5
81	93.0
82	75.0
83	58.0
84	42.0
85	28.5
86	17.0
87	10.0
88	7.0
89	6.5
90	6.0
91	4.0
92	2.5
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	9.716204248215984E-5
4	0.015076310258481803
5	0.010542081609314342
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.009460098874232036
26-27	0.05208061573742036
28-29	0.08117448363243371
30-31	0.06945593768722036
32-33	0.03302094816736178
34-35	0.004430947960107433
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.733904248972251E-4
56-57	5.519973489882155E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	4.805134035850336E-5
72-73	0.015506481670523556
74-75	0.026672580411022214
76-77	0.0012955696857264417
78-79	0.0
80-81	2.0187723623199774E-5
82-83	0.013792397083928163
84-85	0.05025397530485024
86-87	0.03937975992823531
88-89	0.010100792001099606
90-91	3.197989104451121E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	83.0
20-21	360.0
22-23	992.0
24-25	2002.0
26-27	3002.0
28-29	5748.0
30-31	13057.0
32-33	17272.0
34-35	23748.0
36-37	34047.0
38-39	39202.0
40-41	41757.0
42-43	50673.0
44-45	60286.0
46-47	59386.0
48-49	52583.0
50-51	45498.0
52-53	43772.0
54-55	51090.0
56-57	56695.0
58-59	56428.0
60-61	57269.0
62-63	59931.0
64-65	61367.0
66-67	67251.0
68-69	55920.0
70-71	51112.0
72-73	48147.0
74-75	48878.0
76-77	48652.0
78-79	52204.0
80-81	52910.0
82-83	50258.0
84-85	49844.0
86-87	52891.0
88-89	55853.0
90-91	58641.0
92-93	60199.0
94-95	70645.0
96-97	194835.0
98-99	362332.0
100-101	3958431.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.46865362058868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.4083845395215	78.1749128714487
2	6.996320544301033	12.099248355275488
3	1.5098059702297726	3.9165266842228514
4	0.5232260511784196	1.8097060873846067
5	0.21603881067121777	0.9340292544266733
6	0.11434530422236278	0.5932370702366595
7	0.06586576953081853	0.3986727087709709
8	0.04083113297286821	0.2824490475173711
9	0.02703135376262927	0.2103628288856748
>10	0.09447792056180741	1.301230271069078
>50	0.0030469783435150685	0.17552284081292005
>100	6.256247039886328E-4	0.10410197994905808
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.619367374702664E-5
2	0.0	8.09683687351332E-5	0.0	0.0	1.619367374702664E-5
3	0.0	8.09683687351332E-5	0.0	0.0	1.619367374702664E-5
4	0.0	1.1335571622918648E-4	0.0	0.0	1.619367374702664E-5
5	0.0	1.2954938997621312E-4	0.0	3.238734749405328E-5	1.619367374702664E-5
6	0.0	1.2954938997621312E-4	0.0	3.238734749405328E-5	1.619367374702664E-5
7	0.0	1.2954938997621312E-4	0.0	4.8581021241079914E-5	1.619367374702664E-5
8	0.0	1.4574306372323976E-4	0.0	4.8581021241079914E-5	1.619367374702664E-5
9	0.0	3.076798011935061E-4	0.0	6.477469498810656E-5	1.619367374702664E-5
10-11	0.0	3.076798011935061E-4	0.0	6.477469498810656E-5	1.619367374702664E-5
12-13	0.0	3.076798011935061E-4	0.0	6.477469498810656E-5	1.619367374702664E-5
14-15	8.09683687351332E-6	4.129386805491793E-4	0.0	8.09683687351332E-5	2.429051062053996E-5
16-17	1.619367374702664E-5	4.2103551742269263E-4	0.0	9.716204248215983E-5	3.238734749405328E-5
18-19	1.619367374702664E-5	4.291323542962059E-4	0.0	1.3764622684972645E-4	6.477469498810656E-5
20-21	1.619367374702664E-5	4.534228649167459E-4	0.0	1.619367374702664E-4	6.477469498810656E-5
22-23	1.619367374702664E-5	5.101007230313391E-4	0.0	1.7003357434377972E-4	7.287153186161988E-5
24-25	1.619367374702664E-5	5.667785811459324E-4	0.0	1.9432408496431966E-4	8.09683687351332E-5
26-27	1.619367374702664E-5	6.153596023870122E-4	0.0	2.5909877995242624E-4	8.09683687351332E-5
28-29	1.619367374702664E-5	6.153596023870122E-4	0.0	6.801342973751189E-4	8.09683687351332E-5
30-31	3.238734749405328E-5	6.639406236280922E-4	0.0	0.0027934087213620953	8.09683687351332E-5
32-33	3.238734749405328E-5	6.639406236280922E-4	0.0	0.006121208676376069	9.716204248215983E-5
34-35	3.238734749405328E-5	8.015868504778186E-4	0.0	0.010614953141175963	9.716204248215983E-5
36-37	3.238734749405328E-5	9.554267510745717E-4	0.0	0.018169301944163888	1.0525887935567316E-4
38-39	3.238734749405328E-5	9.716204248215983E-4	0.0	0.03327799955013974	1.1335571622918648E-4
40-41	3.238734749405328E-5	0.0010202014460626782	0.0	0.048993959921629096	1.1335571622918648E-4
42-43	3.238734749405328E-5	0.0010282982829361916	0.0	0.060370015728915316	1.2145255310269979E-4
44-45	3.238734749405328E-5	0.0011740413466594314	0.0	0.0742156067826231	1.2954938997621312E-4
46-47	3.238734749405328E-5	0.0013116875735091577	0.0	0.08865226692809733	1.3764622684972645E-4
48-49	3.238734749405328E-5	0.001319784410382671	0.0	0.10278124727237808	1.7813041121729302E-4
50-51	3.238734749405328E-5	0.0014331401266118576	0.0	0.11661874148921234	1.7813041121729302E-4
52-53	3.238734749405328E-5	0.001441236963485371	0.0	0.14609932454567434	1.7813041121729302E-4
54-55	3.238734749405328E-5	0.001441236963485371	0.0	0.18021939513065946	1.8622724809080633E-4
56-57	3.238734749405328E-5	0.001441236963485371	0.0	0.21450140245311486	2.1051775871134632E-4
58-59	3.238734749405328E-5	0.0014493338003588843	0.0	0.23973114615098234	2.3480826933188625E-4
60-61	3.238734749405328E-5	0.001473624310979424	0.0	0.2586939381087505	2.4290510620539958E-4
62-63	3.238734749405328E-5	0.0015383990059675307	0.0	0.27320346978608645	2.7529245369945285E-4
64-65	3.238734749405328E-5	0.0016841420696907705	0.0	0.2787498030444431	2.7529245369945285E-4
66-67	3.238734749405328E-5	0.0016841420696907705	0.0	0.2842961363027997	2.833892905729662E-4
68-69	3.238734749405328E-5	0.0017084325803113105	0.0	0.2897453075186741	2.914861274464795E-4
70-71	3.238734749405328E-5	0.0017165294171848236	0.0	0.2946681843377702	3.076798011935061E-4
72-73	3.238734749405328E-5	0.001724626254058337	0.0	0.29917812247631714	3.5626082243458605E-4
74-75	3.238734749405328E-5	0.0017651104384259035	0.0	0.30263547182130734	3.5626082243458605E-4
76-77	3.238734749405328E-5	0.001846078807161037	0.0	0.3057122698332424	3.643576593080994E-4
78-79	3.238734749405328E-5	0.00190275666527563	0.0	0.3077040917041267	3.724544961816127E-4
80-81	3.238734749405328E-5	0.0019189503390226567	0.0	0.3084570975333634	3.724544961816127E-4
82-83	3.238734749405328E-5	0.0019351440127696835	0.0	0.30899958560388885	3.886481699286393E-4
84-85	3.238734749405328E-5	0.0019594345233902234	0.0	0.3092343938732207	3.886481699286393E-4
86-87	3.238734749405328E-5	0.0019594345233902234	0.0	0.3093315559157028	3.9674500680215264E-4
88	3.238734749405328E-5	0.0020404028921253564	0.0	0.3093477495894499	4.0484184367566597E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9510	0.0	50.72586	1
CTTATAC	7710	0.0	37.80407	1
GTATCAA	17400	0.0	37.26516	1
TATACAC	10290	0.0	30.583326	3
TCAACGC	21235	0.0	29.938362	4
ATCAACG	21385	0.0	29.708614	3
TATCAAC	21875	0.0	29.274872	2
CAACGCA	21780	0.0	29.166752	5
TTATACA	10355	0.0	28.96357	2
AACGCAG	22160	0.0	28.739119	6
ACGCAGA	25240	0.0	25.232124	7
CGCAGAG	26555	0.0	24.014442	8
GTGGTAT	4540	0.0	24.005331	1
TACACAT	13255	0.0	22.879305	5
TGGTATC	4420	0.0	22.65009	2
ACACATC	12970	0.0	22.532427	6
ACATCTC	13260	0.0	21.975937	8
GCAGAGT	29255	0.0	21.711487	9
GAGTACT	18255	0.0	20.231115	12-13
CACATCT	14360	0.0	20.086678	7
>>END_MODULE