FastQCFastQC Report
Fri 27 May 2016
SRR2139139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7483199
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121050.049.930371
GTATCAA221700.036.7822841
TCAACGC267300.029.763724
ATCAACG268550.029.625183
CAACGCA271500.029.3462945
TATCAAC275850.029.1018492
AACGCAG275300.028.9694296
ACGCAGA313450.025.4435637
CGCAGAG319400.024.9828228
CTTATAC76700.023.546691
GCAGAGT363700.021.8468079
GTGGTAT57100.021.6293811
TGGTATC55250.021.1287672
GAGTACT225000.020.37282612-13
GTACATG245450.020.2130741
TACATGG243650.019.9804952
CAGAGTA357050.019.36391810-11
ACATGGG247050.019.3117493
TATACAC110700.019.10383
AGAGTAC337800.018.88383310-11
Produced by FastQC (version 0.11.5)