##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139139_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7483199
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81394147075335	33.0	33.0	33.0	33.0	33.0
2	32.80847108302211	33.0	33.0	33.0	33.0	33.0
3	32.80859696501456	33.0	33.0	33.0	33.0	33.0
4	36.75523302266851	37.0	37.0	37.0	37.0	37.0
5	36.76989105862346	37.0	37.0	37.0	37.0	37.0
6	36.77134738766134	37.0	37.0	37.0	37.0	37.0
7	36.75895549483583	37.0	37.0	37.0	37.0	37.0
8	36.75927501059373	37.0	37.0	37.0	37.0	37.0
9	36.74662801296611	37.0	37.0	37.0	37.0	37.0
10-11	36.72300222137618	37.0	37.0	37.0	37.0	37.0
12-13	36.70757332525835	37.0	37.0	37.0	37.0	37.0
14-15	38.977229858513724	40.0	40.0	40.0	37.0	40.0
16-17	38.932513621514005	40.0	40.0	40.0	37.0	40.0
18-19	38.974491457463586	40.0	40.0	40.0	37.0	40.0
20-21	39.00923971030005	40.0	40.0	40.0	37.0	40.0
22-23	39.03722898423676	40.0	40.0	40.0	37.0	40.0
24-25	39.02514048113514	40.0	40.0	40.0	37.0	40.0
26-27	38.93648378129967	40.0	40.0	40.0	37.0	40.0
28-29	38.862234620279295	40.0	40.0	40.0	37.0	40.0
30-31	38.792667313896104	40.0	37.0	40.0	37.0	40.0
32-33	38.75277829265006	40.0	37.0	40.0	37.0	40.0
34-35	38.72826418217988	40.0	37.0	40.0	37.0	40.0
36-37	38.69503234402923	40.0	37.0	40.0	37.0	40.0
38-39	38.646966630551745	40.0	37.0	40.0	37.0	40.0
40-41	38.60835978673852	40.0	37.0	40.0	37.0	40.0
42-43	38.55121164651772	40.0	37.0	40.0	37.0	40.0
44-45	38.46595207346847	40.0	37.0	40.0	37.0	40.0
46-47	38.38165331226787	40.0	37.0	40.0	37.0	40.0
48-49	38.33706411563156	40.0	37.0	40.0	37.0	40.0
50-51	38.285826404433124	40.0	37.0	40.0	37.0	40.0
52-53	38.22544688720674	40.0	37.0	40.0	37.0	40.0
54-55	38.154657738054034	40.0	37.0	40.0	37.0	40.0
56-57	38.05275938466073	40.0	37.0	40.0	37.0	40.0
58-59	37.92354478671292	40.0	37.0	40.0	33.0	40.0
60-61	37.757726595084335	40.0	37.0	40.0	33.0	40.0
62-63	37.57411911621489	38.5	37.0	40.0	33.0	40.0
64-65	37.356727839022135	37.0	37.0	40.0	33.0	40.0
66-67	37.127435726715035	37.0	37.0	40.0	33.0	40.0
68-69	36.865776801928575	37.0	37.0	40.0	33.0	40.0
70-71	36.61122304626463	37.0	37.0	40.0	33.0	40.0
72-73	36.37268314151228	37.0	37.0	37.0	33.0	40.0
74-75	36.11216280195107	37.0	37.0	37.0	33.0	40.0
76-77	34.46632769831183	35.0	33.0	37.0	30.0	37.0
78-79	35.71333019055736	37.0	35.0	37.0	33.0	37.0
80-81	35.832139648441306	37.0	37.0	37.0	33.0	37.0
82-83	35.73927738389415	37.0	37.0	37.0	33.0	37.0
84-85	35.62909621318798	37.0	37.0	37.0	33.0	37.0
86-87	35.53330702420363	37.0	37.0	37.0	33.0	37.0
88-89	35.438862788145286	37.0	33.0	37.0	33.0	37.0
90-91	35.37756029349283	37.0	33.0	37.0	33.0	37.0
92-93	35.307313606115756	37.0	33.0	37.0	33.0	37.0
94-95	35.26842512626693	37.0	33.0	37.0	33.0	37.0
96-97	35.2296726008236	37.0	33.0	37.0	33.0	37.0
98-99	35.193830104216666	37.0	33.0	37.0	33.0	37.0
100	35.125497377539496	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	4.0
19	10.0
20	23.0
21	107.0
22	462.0
23	1294.0
24	3177.0
25	6654.0
26	12286.0
27	20400.0
28	30527.0
29	44870.0
30	62362.0
31	85473.0
32	115228.0
33	159337.0
34	229493.0
35	353586.0
36	688189.0
37	2093063.0
38	3420828.0
39	155824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.97533701829926	20.116837732098265	14.353740960249754	23.554084289352723
2	14.585767397071761	20.93035344910646	41.94277073214276	22.541108421679017
3	17.93268784255923	26.385400888256953	30.816649840074994	24.865261429108823
4	12.249385668275035	18.341393889480965	38.92096538491662	30.48825505732738
5	13.116056394423639	37.76935145290176	35.605197871102625	13.509394281571977
6	29.402465442920867	37.99491367261515	18.95347163692961	13.649149247534375
7	26.04648092346602	33.06555124352567	22.99626135827739	17.891706474730928
8	24.334806544634187	36.4652069255408	21.20617131790829	17.993815211916722
9	25.527317929136988	16.78699176648917	21.232964671927075	36.452725632446764
10-11	24.02380987061817	26.766807885237316	28.871449229133155	20.337933015011362
12-13	25.126333002770608	24.490207997943124	28.89541625179285	21.48804274749342
14-15	22.280778581459614	25.774097414755374	26.805380960736176	25.139743043048835
16-17	21.56421337986602	28.946590889805286	27.41097089627043	22.07822483405827
18-19	21.511361651614504	27.872792104018618	29.44645064229883	21.169395602068047
20-21	22.406246469085342	26.930928811214578	29.435809366322633	21.227015353377446
22-23	22.1589874850951	27.07083573562521	29.24240067208041	21.527776107199283
24-25	21.872867299702804	27.248104240408104	29.309179228974656	21.569849230914436
26-27	21.746499239148264	27.504783586045278	29.251451397936297	21.49726577687016
28-29	21.697684944645626	27.45293166191386	29.282155501030527	21.56722789240999
30-31	22.04897742866556	27.246805997612555	29.212854232751823	21.491362340970056
32-33	21.766139397303675	27.4283251708008	29.19138385193063	21.614151579964897
34-35	21.872163576238492	27.359365901560572	29.242865746154855	21.52560477604608
36-37	21.815632917653662	27.533562956911734	29.01113841376346	21.639665711671142
38-39	21.714177419916584	27.410141522782087	29.14854504610401	21.727136011197313
40-41	21.933037303048675	27.284424410772708	29.066592293806405	21.715945992372216
42-43	21.93027617338098	27.378529606822326	28.862374393171603	21.82881982662509
44-45	21.93906426371878	27.582964356415374	28.59835104383077	21.879620336035075
46-47	22.14578813148537	27.521403566954994	28.123415288637148	22.209393012922483
48-49	22.085778216768443	27.686599056321736	28.03881299331795	22.188809733591867
50-51	22.055785601928825	27.824692062762907	27.968405790468896	22.151116544839365
52-53	22.14227529547912	27.793529402749467	27.966965155542034	22.09723014622938
54-55	22.135287691903518	27.79687044433361	27.825640581790605	22.242201281972264
56-57	22.26783876622502	27.91272440685407	27.636928205901178	22.182508621019732
58-59	22.058853417124112	28.077010887391136	27.83912040453843	22.02501529094632
60-61	22.279836701736276	28.13023218480501	27.605225107164205	21.984706006294513
62-63	22.114393085806228	28.26706132402717	27.58943247922841	22.029113110938194
64-65	22.114834165672413	28.13456983506686	27.576950109810127	22.1736458894506
66-67	22.247206253842904	28.072140754678777	27.407896816283795	22.272756175194523
68-69	22.219842610114597	27.947959053507333	27.540912748986422	22.291285587391645
70-71	22.204873373019876	27.695253380082736	27.62387593330643	22.47599731359096
72-73	22.29368930975957	27.687357337304313	27.572777332219832	22.446176020716283
74-75	22.189213493479528	27.814556865453817	27.546821185461944	22.449408455604708
76-77	22.33734974373258	27.73998131264203	27.47200690424267	22.450662039382717
78-79	22.33947421648132	27.69251316533078	27.41729788078164	22.550714737406267
80-81	22.289692437747643	27.749053559307313	27.468744854273865	22.492509148671182
82-83	22.363585757298708	27.71489595873204	27.558594430755782	22.362923853213466
84-85	22.50723012203531	27.638880182434573	27.451955902608944	22.401933792921174
86-87	22.347296697703914	27.70934300851967	27.525493005188935	22.41786728858748
88-89	22.46604866587377	27.602970255702548	27.496991138732536	22.433989939691145
90-91	22.50667507073615	27.627808455612808	27.48121075773507	22.38430571591597
92-93	22.515220676825365	27.505324693705173	27.58715968092973	22.392294948539732
94-95	22.384466933243438	27.58241667163653	27.61823571053676	22.414880684583274
96-97	22.437838550970195	27.664209548795654	27.673540027754733	22.22441187247942
98-99	23.591676741703342	29.52205588754948	27.889919811581255	18.996347559165926
100	22.462792334064705	30.530455641076397	21.706828378133352	25.299923646725542
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1760.0
1	1582.5
2	2249.0
3	3302.5
4	4655.5
5	7701.0
6	10195.5
7	12058.5
8	13419.0
9	13414.5
10	12282.5
11	11156.0
12	12287.0
13	16350.0
14	25719.5
15	38233.0
16	48612.5
17	55163.5
18	56668.0
19	53665.5
20	48330.0
21	42533.0
22	38926.5
23	39702.0
24	44935.5
25	53784.5
26	65206.5
27	78269.0
28	93094.5
29	110689.5
30	128295.5
31	144582.5
32	160617.0
33	176188.5
34	191411.5
35	205464.5
36	221286.5
37	237095.5
38	249128.5
39	257928.5
40	267106.5
41	275452.0
42	281167.0
43	287752.0
44	292644.0
45	299547.5
46	308707.0
47	315514.0
48	313556.5
49	307983.5
50	304529.5
51	294648.5
52	280981.5
53	265557.0
54	249702.5
55	232245.0
56	213508.5
57	194184.0
58	172425.0
59	150057.5
60	125881.0
61	102584.0
62	81357.5
63	62716.0
64	47627.0
65	35788.5
66	27053.5
67	20379.0
68	15508.5
69	11432.5
70	8238.5
71	5961.0
72	4146.5
73	2767.5
74	1790.0
75	1186.5
76	790.0
77	500.5
78	324.5
79	208.5
80	133.5
81	88.5
82	63.0
83	40.0
84	26.5
85	18.5
86	16.0
87	15.0
88	12.0
89	9.0
90	6.5
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	5.345307534919224E-5
4	0.014499146688468393
5	0.011064786597282794
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.009377153177835672
26-27	0.05406120241581235
28-29	0.08415348589812058
30-31	0.07156399405575509
32-33	0.034032073786368924
34-35	0.004659261224308416
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.6426984877264377E-4
56-57	8.818815884362982E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.1530612598813004E-5
72-73	0.015332308685068251
74-75	0.026385548724174736
76-77	0.0010550381765564187
78-79	0.0
80-81	8.287026808697468E-6
82-83	0.013847796713338965
84-85	0.053212813577776436
86-87	0.04121055116779918
88-89	0.01042535987840082
90-91	2.6306554488503244E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	114.0
20-21	431.0
22-23	1012.0
24-25	2364.0
26-27	2848.0
28-29	6680.0
30-31	16135.0
32-33	21110.0
34-35	28706.0
36-37	41478.0
38-39	49338.0
40-41	52480.0
42-43	62449.0
44-45	75021.0
46-47	73544.0
48-49	64557.0
50-51	55589.0
52-53	52291.0
54-55	57563.0
56-57	62953.0
58-59	62627.0
60-61	63873.0
62-63	64099.0
64-65	67153.0
66-67	73090.0
68-69	66666.0
70-71	63072.0
72-73	59954.0
74-75	60057.0
76-77	61519.0
78-79	64531.0
80-81	65403.0
82-83	63397.0
84-85	62958.0
86-87	66415.0
88-89	70749.0
90-91	74454.0
92-93	76895.0
94-95	84086.0
96-97	236078.0
98-99	440236.0
100-101	4809224.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.54862357999424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.04527789686742	75.28655682478225
2	7.675097032656927	12.978377799080826
3	1.8808768980733699	4.770766585665378
4	0.661563633107865	2.237371783594012
5	0.29586971169699533	1.250768844149534
6	0.15491729745215665	0.7858826560987436
7	0.08863098222710265	0.5245539287691123
8	0.05261746626578303	0.3558987479230988
9	0.03278702250629825	0.24948878617744294
>10	0.11023352302864213	1.3983783689728084
>50	0.0018702702280053915	0.1043079041680117
>100	2.3313885000438822E-4	0.04137443305468832
>500	2.512703946076245E-5	0.01627333756402512
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3363268837298059E-5
2	0.0	0.0	0.0	0.0	1.3363268837298059E-5
3	0.0	1.3363268837298059E-5	0.0	0.0	1.3363268837298059E-5
4	0.0	1.2026941953568253E-4	0.0	0.0	1.3363268837298059E-5
5	0.0	1.336326883729806E-4	0.0	1.3363268837298059E-5	1.3363268837298059E-5
6	0.0	1.336326883729806E-4	0.0	1.3363268837298059E-5	2.6726537674596118E-5
7	0.0	1.4699595721027865E-4	0.0	1.3363268837298059E-5	2.6726537674596118E-5
8	0.0	1.4699595721027865E-4	0.0	1.3363268837298059E-5	4.008980651189418E-5
9	0.0	2.5390210790866315E-4	0.0	2.6726537674596118E-5	4.008980651189418E-5
10-11	0.0	2.5390210790866315E-4	0.0	6.68163441864903E-5	4.008980651189418E-5
12-13	0.0	2.6058374232731214E-4	0.0	8.017961302378835E-5	4.008980651189418E-5
14-15	0.0	3.340817209324515E-4	0.0	1.0690615069838447E-4	7.349797860513932E-5
16-17	0.0	3.608082586070476E-4	0.0	1.4699595721027865E-4	8.017961302378835E-5
18-19	0.0	4.1426133395623986E-4	0.0	2.873102800019083E-4	8.017961302378835E-5
20-21	0.0	4.944409469800282E-4	0.0	3.8753479628164374E-4	8.017961302378835E-5
22-23	0.0	5.278491190732733E-4	0.0	8.619308400057248E-4	8.686124744243738E-5
24-25	0.0	5.946654632597636E-4	0.0	0.002245029164666074	9.354288186108641E-5
26-27	0.0	6.147103665157108E-4	0.0	0.0055123483953854495	9.354288186108641E-5
28-29	0.0	6.213920009343597E-4	0.0	0.01576197559359306	9.354288186108641E-5
30-31	0.0	6.81526710702201E-4	0.0	0.0175660168866283	9.354288186108641E-5
32-33	0.0	6.948899795394991E-4	0.0	0.02093356063362741	9.354288186108641E-5
34-35	0.0	7.683879581446384E-4	0.0	0.02501603926342197	1.0690615069838447E-4
36-37	0.0	8.285226679124797E-4	0.0	0.03197830232765426	1.0690615069838447E-4
38-39	0.0	8.485675711684267E-4	0.0	0.04866902510543954	1.0690615069838447E-4
40-41	0.0	9.487920874481622E-4	0.0	0.06606131949718295	1.0690615069838447E-4
42-43	0.0	9.755186251227583E-4	0.0	0.07785440424609849	1.0690615069838447E-4
44-45	0.0	0.0010690615069838447	0.0	0.09145153028804927	1.0690615069838447E-4
46-47	0.0	0.001149241120007633	0.0	0.10617785254675173	1.2026941953568253E-4
48-49	0.0	0.001149241120007633	0.0	0.12010906030963495	1.336326883729806E-4
50-51	0.0	0.0012828738083806137	0.0	0.1340269048036809	1.336326883729806E-4
52-53	0.0	0.0013096003460552098	0.0	0.16718117478901737	1.4699595721027865E-4
54-55	0.0	0.001322963614892508	0.0	0.206729768913001	1.603592260475767E-4
56-57	0.0	0.001336326883729806	0.0	0.24632513447791513	1.603592260475767E-4
58-59	0.0	0.001343008518148455	0.0	0.2707398266436587	1.603592260475767E-4
60-61	0.0	0.0013764166902417	0.0	0.2869695166465572	1.7372249488487477E-4
62-63	0.0	0.0014031432279162962	0.0	0.29862896870709976	1.8708576372217283E-4
64-65	0.0	0.0016436820669876613	0.0	0.3043217212317887	1.9376739814082187E-4
66-67	0.0	0.0016503637014063104	0.0	0.30968707366996384	2.0044903255947088E-4
68-69	0.0	0.0016837718734995554	0.0	0.3150390628393017	2.0044903255947088E-4
70-71	0.0	0.0017104984111741516	0.0	0.3202707825891039	2.071306669781199E-4
72-73	0.0	0.0017238616800114496	0.0	0.3242329917993628	2.27175570234067E-4
74-75	0.0	0.001763951486523344	0.0	0.32793461726729434	2.27175570234067E-4
76-77	0.0	0.0018708576372217282	0.0	0.33092798948684915	2.27175570234067E-4
78-79	0.0	0.0019243107125709205	0.0	0.3329792512533744	2.27175570234067E-4
80-81	0.0	0.0019243107125709205	0.0	0.3335805983510528	2.4053883907136506E-4
82-83	0.0	0.0019309923469895695	0.0	0.33410176583570744	2.672653767459612E-4
84-85	0.0	0.0019510372502455167	0.0	0.3343088965026856	2.672653767459612E-4
86-87	0.0	0.002017853594432007	0.0	0.3343690312124534	2.672653767459612E-4
88	0.0	0.0021782128204795837	0.0	0.33438907611570934	2.672653767459612E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12105	0.0	49.93037	1
GTATCAA	22170	0.0	36.782284	1
TCAACGC	26730	0.0	29.76372	4
ATCAACG	26855	0.0	29.62518	3
CAACGCA	27150	0.0	29.346294	5
TATCAAC	27585	0.0	29.101849	2
AACGCAG	27530	0.0	28.969429	6
ACGCAGA	31345	0.0	25.443563	7
CGCAGAG	31940	0.0	24.982822	8
CTTATAC	7670	0.0	23.54669	1
GCAGAGT	36370	0.0	21.846807	9
GTGGTAT	5710	0.0	21.629381	1
TGGTATC	5525	0.0	21.128767	2
GAGTACT	22500	0.0	20.372826	12-13
GTACATG	24545	0.0	20.213074	1
TACATGG	24365	0.0	19.980495	2
CAGAGTA	35705	0.0	19.363918	10-11
ACATGGG	24705	0.0	19.311749	3
TATACAC	11070	0.0	19.1038	3
AGAGTAC	33780	0.0	18.883833	10-11
>>END_MODULE