FastQCFastQC Report
Fri 27 May 2016
SRR2139138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6754569
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102350.053.296321
GTATCAA193650.037.804851
ATCAACG233400.030.7705753
TCAACGC241100.029.8568534
CAACGCA245900.029.2707965
TATCAAC248900.029.1084332
AACGCAG247900.029.0494846
ACGCAGA252400.028.4981967
CTTATAC97650.027.2190151
CGCAGAG292850.024.5474958
TATACAC122450.023.460523
GTGGTAT46100.022.7516921
TGGTATC43950.022.4270572
GCAGAGT329950.021.6979969
TTATACA124450.021.6959362
GAGTACT203850.020.62795412-13
CAGAGTA322650.019.37614610-11
AGAGTAC304900.019.17817710-11
ACACATC137200.019.0307716
GTACTTT222650.018.86754814-15
Produced by FastQC (version 0.11.5)