FastQCFastQC Report
Fri 27 May 2016
SRR2139137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6875187
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105400.051.655661
GTATCAA198350.038.597431
ATCAACG237100.031.7098273
TCAACGC237800.031.6164864
CAACGCA242600.031.0401885
TATCAAC245950.030.8830552
AACGCAG247450.030.4106946
ACGCAGA281200.026.69977
CGCAGAG287900.026.1379878
GTGGTAT50950.023.4282511
GCAGAGT325600.022.9138079
TGGTATC49150.022.8884852
GAGTACT194050.021.07090612-13
ATCTCGT106400.020.90775394
TATCTCG120850.020.57344694
GTACATG226400.020.2740521
CAGAGTA318800.019.9756610-11
TACATGG227550.019.6243722
AGAGTAC302500.019.09370410-11
ACATGGG232200.018.7505933
Produced by FastQC (version 0.11.5)