##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139137_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6875187
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.818034767636135	33.0	33.0	33.0	33.0	33.0
2	32.813948478783196	33.0	33.0	33.0	33.0	33.0
3	32.8151293921169	33.0	33.0	33.0	33.0	33.0
4	36.76332658297149	37.0	37.0	37.0	37.0	37.0
5	36.77588216873228	37.0	37.0	37.0	37.0	37.0
6	36.77841824520555	37.0	37.0	37.0	37.0	37.0
7	36.76676503490014	37.0	37.0	37.0	37.0	37.0
8	36.76728763886713	37.0	37.0	37.0	37.0	37.0
9	36.754535985712096	37.0	37.0	37.0	37.0	37.0
10-11	36.731609263864385	37.0	37.0	37.0	37.0	37.0
12-13	36.71568147891831	37.0	37.0	37.0	37.0	37.0
14-15	39.00170097191538	40.0	40.0	40.0	37.0	40.0
16-17	38.95550186489473	40.0	40.0	40.0	37.0	40.0
18-19	38.99961921035748	40.0	40.0	40.0	37.0	40.0
20-21	39.03176598915162	40.0	40.0	40.0	37.0	40.0
22-23	39.06072784632343	40.0	40.0	40.0	37.0	40.0
24-25	39.05103166728643	40.0	40.0	40.0	37.0	40.0
26-27	38.966720714941204	40.0	40.0	40.0	37.0	40.0
28-29	38.8977777410519	40.0	40.0	40.0	37.0	40.0
30-31	38.831171883280874	40.0	38.5	40.0	37.0	40.0
32-33	38.78846010743503	40.0	37.0	40.0	37.0	40.0
34-35	38.7631238477457	40.0	37.0	40.0	37.0	40.0
36-37	38.72746467996792	40.0	37.0	40.0	37.0	40.0
38-39	38.68205324417633	40.0	37.0	40.0	37.0	40.0
40-41	38.64190334630118	40.0	37.0	40.0	37.0	40.0
42-43	38.58251274620322	40.0	37.0	40.0	37.0	40.0
44-45	38.4938612324714	40.0	37.0	40.0	37.0	40.0
46-47	38.4030190740703	40.0	37.0	40.0	37.0	40.0
48-49	38.34920239479651	40.0	37.0	40.0	37.0	40.0
50-51	38.29749059129652	40.0	37.0	40.0	37.0	40.0
52-53	38.24415492828763	40.0	37.0	40.0	37.0	40.0
54-55	38.17602625817872	40.0	37.0	40.0	37.0	40.0
56-57	38.06662487691064	40.0	37.0	40.0	37.0	40.0
58-59	37.943276452237384	40.0	37.0	40.0	33.0	40.0
60-61	37.780814448952725	40.0	37.0	40.0	33.0	40.0
62-63	37.60435229291666	38.5	37.0	40.0	33.0	40.0
64-65	37.3923476077636	37.0	37.0	40.0	33.0	40.0
66-67	37.161536141055976	37.0	37.0	40.0	33.0	40.0
68-69	36.8983934843463	37.0	37.0	40.0	33.0	40.0
70-71	36.64413359528581	37.0	37.0	40.0	33.0	40.0
72-73	36.404632477274795	37.0	37.0	37.0	33.0	40.0
74-75	36.13634484330316	37.0	37.0	37.0	33.0	40.0
76-77	34.47391657332651	35.0	33.0	37.0	30.0	37.0
78-79	35.70996581662409	37.0	35.0	37.0	33.0	37.0
80-81	35.820762730034794	37.0	37.0	37.0	33.0	37.0
82-83	35.727557585531514	37.0	37.0	37.0	33.0	37.0
84-85	35.6260466312645	37.0	37.0	37.0	33.0	37.0
86-87	35.53304039310953	37.0	37.0	37.0	33.0	37.0
88-89	35.44167359342666	37.0	33.0	37.0	33.0	37.0
90-91	35.38995369698327	37.0	33.0	37.0	33.0	37.0
92-93	35.332502308657894	37.0	33.0	37.0	33.0	37.0
94-95	35.27482319303403	37.0	33.0	37.0	33.0	37.0
96-97	35.228232475401114	37.0	33.0	37.0	33.0	37.0
98-99	35.19384693321588	37.0	33.0	37.0	33.0	37.0
100	35.12342777727648	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	8.0
20	21.0
21	95.0
22	417.0
23	1208.0
24	3006.0
25	6032.0
26	11212.0
27	18602.0
28	28836.0
29	41212.0
30	57700.0
31	78354.0
32	105788.0
33	144773.0
34	208380.0
35	320903.0
36	622709.0
37	1938089.0
38	3156452.0
39	131387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58942876753752	20.290502643782634	14.56609107505003	23.55397751362981
2	14.492638527504779	21.15700998387389	41.69636404071627	22.653987447905052
3	17.667302140845457	26.64294947289388	31.065480312445594	24.624268073815074
4	12.327788475624065	18.63690737087509	39.08897021417982	29.94633393932103
5	13.17202791668982	37.75168180629327	35.58775722406463	13.488533052952278
6	29.046119036471296	38.16622006063253	19.092411595495513	13.695249307400656
7	25.852402269203733	33.183824672696176	23.182380348345433	17.78139270975466
8	24.39479827966861	36.00995871094124	21.509044044911068	18.086198964479074
9	25.578140638211007	16.75881688745339	21.31766015964366	36.345382314691946
10-11	23.880899530441862	26.75253778551769	29.026010492514605	20.340552191525845
12-13	25.038155325811502	24.507558848944765	28.934252988318722	21.52003283692502
14-15	22.274914122335872	25.791073028268173	26.771729990762434	25.162282858633517
16-17	21.568729694188683	28.983873456823794	27.354761405035237	22.09263544395229
18-19	21.614532084727294	27.817766993101422	29.349172611595876	21.218528310575408
20-21	22.45387739013028	26.907938779792197	29.348179840996767	21.290003989080756
22-23	22.14115270324289	27.03211615591803	29.135554028491445	21.69117711234763
24-25	21.994581171436973	27.177995763916368	29.15653974058626	21.670883324060398
26-27	21.874669361701788	27.325612850942633	29.194947581018354	21.604770206337232
28-29	21.772424777912374	27.212617852024273	29.279676243199166	21.735281126864187
30-31	21.954408247268677	27.015988798708783	29.277800891738554	21.751802062283986
32-33	21.68279152645182	27.368231164571476	29.09346378763553	21.855513521341177
34-35	21.959191267221243	27.31839000561803	29.126238695269063	21.596180031891667
36-37	21.921902159686827	27.32592065364129	29.054074522871858	21.698102663800025
38-39	21.765482892435607	27.31278102351546	29.00110616748745	21.920629916561488
40-41	22.042474238558107	27.141392949800146	29.01953760342136	21.796595208220385
42-43	22.012435388217046	27.218919919020724	28.786583634529244	21.982061058232986
44-45	21.95244601320295	27.42315585548744	28.589983020471543	22.034415110838065
46-47	22.19520166835467	27.33126062963609	28.22803476276902	22.24550293924022
48-49	22.104888293765793	27.612043988080938	28.038197926690735	22.244869791462534
50-51	22.02611211448798	27.77279059952719	27.898222609968442	22.302874676016383
52-53	22.082907715144213	27.627669324773574	27.901640585623678	22.38778237445854
54-55	22.18730797303549	27.410106558977247	28.03630476245951	22.366280705527753
56-57	22.22200549172539	27.291408101765686	27.91200287561491	22.574583530894017
58-59	21.836287477199214	27.25395223411552	28.237934551861482	22.67182573682378
60-61	22.156519969268235	27.52496168375046	28.217867872622275	22.10065047435903
62-63	21.644443419578458	27.834666675148316	28.178586962485397	22.34230294278783
64-65	21.525622881002366	28.04577311947531	27.892399391355738	22.536204608166585
66-67	21.674944989324203	28.409772993796818	27.489816550984457	22.42546546589452
68-69	21.71452512430533	28.334950796961607	27.369692274296114	22.58083180443695
70-71	21.740550944080717	27.806248197915806	27.34828046970998	23.104920388293497
72-73	22.028035061368616	27.34553473962748	27.291083663496536	23.335346535507366
74-75	22.027645187205316	27.415271474493697	27.51102248533739	23.046060852963603
76-77	22.366249662742526	27.264517440053552	26.9430726461136	23.426160251090323
78-79	22.56263877206087	27.280804734107576	26.52659726485708	23.629959228974474
80-81	22.40734973935867	27.65789234789629	26.641497106858107	23.293260805886934
82-83	22.38166971284991	27.44858287229097	26.72714533715288	23.442602077706244
84-85	22.52451903301836	27.7757155711029	26.63956502072258	23.06020037515616
86-87	22.89028720823898	27.65670032796983	26.754546629065736	22.698465834725457
88-89	22.697951659365323	27.67777142173358	27.077043160134373	22.54723375876672
90-91	22.662538859845167	27.35525256151204	26.926641886770074	23.05556669187272
92-93	22.679564197182277	26.854360162703312	27.182571632073614	23.283504008040797
94-95	22.006986233301298	27.27668604100754	27.753399087271703	22.96292863841946
96-97	22.156419062451977	27.215399641525583	27.891903319645618	22.73627797637682
98-99	23.371197124809456	29.017091286478713	28.2570925791439	19.354619009567934
100	22.259214830777616	30.125304536600122	21.969495915626773	25.645984716995486
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1359.0
1	1300.5
2	1899.5
3	2734.5
4	3781.5
5	6264.5
6	8408.5
7	9941.5
8	11192.5
9	11441.5
10	10815.5
11	10151.0
12	11112.0
13	14250.5
14	21742.0
15	32233.5
16	41239.0
17	47026.0
18	48383.0
19	45583.0
20	40674.5
21	35187.5
22	31189.0
23	30981.0
24	34692.5
25	41885.0
26	52283.0
27	64354.0
28	77622.0
29	93543.0
30	109919.5
31	124885.5
32	140078.0
33	155500.0
34	170166.0
35	183436.5
36	198101.0
37	213011.5
38	224261.0
39	233117.0
40	242049.0
41	250180.5
42	256300.0
43	262770.0
44	268393.5
45	273433.0
46	277520.5
47	281383.5
48	290749.0
49	292158.5
50	283163.5
51	275033.5
52	263486.0
53	247061.0
54	229878.5
55	211457.5
56	192358.5
57	172610.0
58	152002.0
59	131820.0
60	110418.0
61	90287.5
62	71595.5
63	54898.0
64	41917.0
65	31952.0
66	24359.5
67	18681.5
68	14256.5
69	10021.0
70	6705.5
71	4820.5
72	3323.5
73	2220.5
74	1493.0
75	989.0
76	659.5
77	417.0
78	253.0
79	165.0
80	105.0
81	66.5
82	51.5
83	41.5
84	27.5
85	16.5
86	13.5
87	13.5
88	10.5
89	7.0
90	5.0
91	4.5
92	4.0
93	2.5
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	8.727035351911155E-5
4	0.014690509509050445
5	0.010661528188251461
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.009239341056015942
26-27	0.052910526552896665
28-29	0.08222843500494986
30-31	0.0707894422270102
32-33	0.03335694686721152
34-35	0.004072357292808202
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.9591192228356164E-4
56-57	6.709931158446988E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.316082519387062E-5
72-73	0.014650995292342194
74-75	0.025537789842502594
76-77	0.0010160311790085566
78-79	0.0
80-81	1.7440907806227677E-5
82-83	0.012995437843102925
84-85	0.05019296933033114
86-87	0.03880721439273604
88-89	0.009530117771280273
90-91	6.466244494801093E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	100.0
20-21	402.0
22-23	923.0
24-25	2927.0
26-27	2835.0
28-29	5375.0
30-31	13063.0
32-33	17535.0
34-35	24222.0
36-37	34248.0
38-39	26488.0
40-41	44865.0
42-43	53464.0
44-45	64914.0
46-47	63086.0
48-49	55923.0
50-51	30911.0
52-53	10297.0
54-55	11507.0
56-57	13502.0
58-59	35837.0
60-61	60141.0
62-63	61146.0
64-65	63679.0
66-67	69774.0
68-69	61715.0
70-71	59729.0
72-73	57387.0
74-75	58582.0
76-77	59076.0
78-79	62075.0
80-81	62968.0
82-83	60785.0
84-85	62087.0
86-87	64140.0
88-89	68537.0
90-91	71985.0
92-93	74326.0
94-95	81054.0
96-97	233798.0
98-99	448155.0
100-101	4521624.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.4393250707164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.42102125081246	76.40071702803604
2	7.624028620064159	13.02783719236214
3	1.7309615342989482	4.436765556416215
4	0.6066086543265562	2.0731293603086587
5	0.25471906055534466	1.0881512308247794
6	0.13688813504787783	0.7017377921207857
7	0.06738958445946003	0.4030404429108612
8	0.03969495813763949	0.2713208345592208
9	0.02763740643696907	0.21251892174117393
>10	0.08870073957405598	1.1367804557164878
>50	0.0018136672285292662	0.10035632851048701
>100	4.580982535173452E-4	0.08788878254046464
>500	5.219387888524636E-5	0.03007871754614584
>1k	2.6096925626960522E-5	0.029677356406562803
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4545058919851925E-5
2	0.0	0.0	0.0	0.0	1.4545058919851925E-5
3	0.0	0.0	0.0	0.0	1.4545058919851925E-5
4	0.0	8.727035351911155E-5	0.0	0.0	1.4545058919851925E-5
5	0.0	1.0181541243896348E-4	0.0	0.0	1.4545058919851925E-5
6	4.3635176759555775E-5	1.0181541243896348E-4	0.0	0.0	1.4545058919851925E-5
7	4.3635176759555775E-5	1.0181541243896348E-4	0.0	0.0	1.4545058919851925E-5
8	4.3635176759555775E-5	1.3090553027866734E-4	0.0	0.0	1.4545058919851925E-5
9	4.3635176759555775E-5	2.181758837977789E-4	0.0	0.0	4.3635176759555775E-5
10-11	7.272529459925963E-5	2.181758837977789E-4	0.0	0.0	4.3635176759555775E-5
12-13	1.0181541243896348E-4	2.181758837977789E-4	0.0	0.0	4.3635176759555775E-5
14-15	1.163604713588154E-4	3.1271876677681644E-4	0.0	5.090770621948174E-5	5.81802356794077E-5
16-17	1.3090553027866734E-4	3.199912962367424E-4	0.0	1.0181541243896348E-4	5.81802356794077E-5
18-19	1.4545058919851925E-4	3.4180888461652026E-4	0.0	1.2363300081874137E-4	5.81802356794077E-5
20-21	1.4545058919851925E-4	3.6362647299629814E-4	0.0	1.3817805973859328E-4	7.272529459925963E-5
22-23	1.4545058919851925E-4	3.85444061376076E-4	0.0	4.2907923813563184E-4	7.999782405918558E-5
24-25	1.4545058919851925E-4	4.072616497558539E-4	0.0	0.0015272311865844523	8.727035351911155E-5
26-27	1.4545058919851925E-4	4.072616497558539E-4	0.0	0.004465333088394541	8.727035351911155E-5
28-29	1.4545058919851925E-4	4.072616497558539E-4	0.0	0.01284328702622925	8.727035351911155E-5
30-31	1.4545058919851925E-4	4.5816935597533566E-4	0.0	0.014690509509050445	8.727035351911155E-5
32-33	1.4545058919851925E-4	4.654418854352616E-4	0.0	0.018995846949326614	8.727035351911155E-5
34-35	1.4545058919851925E-4	5.381671800345212E-4	0.0	0.02442842645589131	8.727035351911155E-5
36-37	1.4545058919851925E-4	6.108924746337809E-4	0.0	0.03463905781762736	8.727035351911155E-5
38-39	1.4545058919851925E-4	6.254375335536328E-4	0.0	0.15953747876239582	8.727035351911155E-5
40-41	1.4545058919851925E-4	7.127078870727444E-4	0.0	0.2875849049633123	8.727035351911155E-5
42-43	1.4545058919851925E-4	7.417980049124482E-4	0.0	0.308529789807899	8.727035351911155E-5
44-45	1.4545058919851925E-4	8.508859468113377E-4	0.0	0.3392416817171664	8.727035351911155E-5
46-47	1.4545058919851925E-4	9.163387119506713E-4	0.0	0.3707608243964855	8.727035351911155E-5
48-49	1.5999564811837116E-4	9.236112414105972E-4	0.0	0.40001093788430775	8.727035351911155E-5
50-51	1.9635829541800098E-4	0.0010254266538495607	0.0	0.42798835871664287	8.727035351911155E-5
52-53	2.0363082487792695E-4	0.0010326991833094867	0.0	0.8652419199652315	1.163604713588154E-4
54-55	2.0363082487792695E-4	0.0010326991833094867	0.0	1.4635383735744205	1.4545058919851925E-4
56-57	2.0363082487792695E-4	0.0010326991833094867	0.0	2.1231567955897055	1.5272311865844522E-4
58-59	2.0363082487792695E-4	0.0010326991833094867	0.0	2.8060618569356732	1.599956481183712E-4
60-61	2.1817588379777886E-4	0.0010472442422293387	0.0	2.988813249734153	1.599956481183712E-4
62-63	2.327209427176308E-4	0.0010472442422293387	0.0	2.9944276424772154	1.599956481183712E-4
64-65	2.3999347217755677E-4	0.001163604713588154	0.0	2.999460232863484	1.599956481183712E-4
66-67	2.618110605573347E-4	0.0011999673608877838	0.0	3.0047764518986906	1.6726817757829716E-4
68-69	2.618110605573347E-4	0.0012363300081874137	0.0	3.0100563082865963	1.745407070382231E-4
70-71	2.690835900172606E-4	0.0012363300081874137	0.0	3.014936175554207	1.8908576595807504E-4
72-73	2.909011783970385E-4	0.0012363300081874137	0.0	3.019256058053403	2.181758837977789E-4
74-75	2.909011783970385E-4	0.0012508750671072655	0.0	3.022368700662251	2.181758837977789E-4
76-77	2.909011783970385E-4	0.0012654201260271175	0.0	3.0253577102702804	2.2544841325770486E-4
78-79	2.909011783970385E-4	0.0012799651849469695	0.0	3.0271903876941817	2.327209427176308E-4
80-81	2.909011783970385E-4	0.0012799651849469695	0.0	3.0281212714650527	2.618110605573347E-4
82-83	2.909011783970385E-4	0.0012872377144068953	0.0	3.028797616704826	3.0544623731689044E-4
84-85	2.909011783970385E-4	0.0012945102438668213	0.0	3.029059427765383	3.0544623731689044E-4
86-87	2.909011783970385E-4	0.0013236003617065253	0.0	3.029153970648362	3.1271876677681644E-4
88	3.0544623731689044E-4	0.001367235538466081	0.0	3.029153970648362	3.199912962367424E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10540	0.0	51.65566	1
GTATCAA	19835	0.0	38.59743	1
ATCAACG	23710	0.0	31.709827	3
TCAACGC	23780	0.0	31.616486	4
CAACGCA	24260	0.0	31.040188	5
TATCAAC	24595	0.0	30.883055	2
AACGCAG	24745	0.0	30.410694	6
ACGCAGA	28120	0.0	26.6997	7
CGCAGAG	28790	0.0	26.137987	8
GTGGTAT	5095	0.0	23.428251	1
GCAGAGT	32560	0.0	22.913807	9
TGGTATC	4915	0.0	22.888485	2
GAGTACT	19405	0.0	21.070906	12-13
ATCTCGT	10640	0.0	20.907753	94
TATCTCG	12085	0.0	20.573446	94
GTACATG	22640	0.0	20.274052	1
CAGAGTA	31880	0.0	19.97566	10-11
TACATGG	22755	0.0	19.624372	2
AGAGTAC	30250	0.0	19.093704	10-11
ACATGGG	23220	0.0	18.750593	3
>>END_MODULE