FastQCFastQC Report
Fri 27 May 2016
SRR2139136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9255533
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC139650.051.1227761
TATACAC162150.045.5614433
GGTATCA221950.044.7793661
TTATACA178600.040.7037732
GTATCAA397000.035.145081
TACACAT247400.030.2079285
ATACACA258150.029.5021554
ATCAACG496800.027.682723
ACACATC262300.027.6555866
TATCAAC500450.027.5299722
TCAACGC502800.027.4010054
AACGCAG504700.027.3329526
CAACGCA505750.027.1689575
ACATCTC289950.025.089548
CACATCT292850.024.7158377
ACGCAGA564950.024.3684127
CGCAGAG569000.024.2092868
CATCTCC327250.022.0430269
GCAGAGT625300.021.9317999
TACATGG374250.020.698632
Produced by FastQC (version 0.11.5)