FastQCFastQC Report
Fri 27 May 2016
SRR2139135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8638458
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA253200.042.6684881
GTATCAA435400.034.3102871
ATCAACG550200.026.8527153
TATCAAC553000.026.8282852
AACGCAG554400.026.7287656
CAACGCA561250.026.3324175
TCAACGC566100.026.1347734
ACGCAGA617600.023.9215247
CGCAGAG619400.023.8321158
GCAGAGT674750.021.7615019
TACATGG391950.020.3245622
GTACATG402750.020.2077621
GAGTACT456650.020.09105112-13
ACATGGG395150.019.650663
ACGGTAC11450.019.0071953
CAGAGTA669350.018.97019210-11
AGAGTAC650600.018.71514110-11
AGTACTT482550.018.43397712-13
GTACTAG24450.018.3061811
CATGGGG253450.018.2914964
Produced by FastQC (version 0.11.5)