##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139135_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8638458
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.718929466346886	33.0	33.0	33.0	33.0	33.0
2	32.650469678732016	33.0	33.0	33.0	33.0	33.0
3	32.721440331133174	33.0	33.0	33.0	33.0	33.0
4	36.70141384029418	37.0	37.0	37.0	37.0	37.0
5	36.70895060206347	37.0	37.0	37.0	37.0	37.0
6	36.70041655582513	37.0	37.0	37.0	37.0	37.0
7	36.68629725351446	37.0	37.0	37.0	37.0	37.0
8	36.68052052808499	37.0	37.0	37.0	37.0	37.0
9	36.67687415971693	37.0	37.0	37.0	37.0	37.0
10-11	36.68288946939373	37.0	37.0	37.0	37.0	37.0
12-13	36.68071489147716	37.0	37.0	37.0	37.0	37.0
14-15	38.87992098821341	40.0	37.0	40.0	37.0	40.0
16-17	38.81665205757787	40.0	37.0	40.0	37.0	40.0
18-19	38.87376306049066	40.0	37.0	40.0	37.0	40.0
20-21	38.892313744969435	40.0	37.0	40.0	37.0	40.0
22-23	38.89641399819281	40.0	37.0	40.0	37.0	40.0
24-25	38.88750039620942	40.0	37.0	40.0	37.0	40.0
26-27	38.80498565520901	40.0	37.0	40.0	37.0	40.0
28-29	38.69977086150514	40.0	37.0	40.0	37.0	40.0
30-31	38.53058889164281	40.0	37.0	40.0	37.0	40.0
32-33	38.42313904148483	40.0	37.0	40.0	37.0	40.0
34-35	38.39120156888391	40.0	37.0	40.0	37.0	40.0
36-37	38.36501470152133	40.0	37.0	40.0	37.0	40.0
38-39	38.318792788753086	40.0	37.0	40.0	37.0	40.0
40-41	38.28886258690844	40.0	37.0	40.0	37.0	40.0
42-43	38.25749501484939	40.0	37.0	40.0	37.0	40.0
44-45	38.15911257416527	40.0	37.0	40.0	37.0	40.0
46-47	37.77868000562597	40.0	37.0	40.0	33.0	40.0
48-49	37.95155041542998	40.0	37.0	40.0	33.0	40.0
50-51	37.96388207289179	40.0	37.0	40.0	33.0	40.0
52-53	37.92923735924829	40.0	37.0	40.0	33.0	40.0
54-55	37.83500464575052	40.0	37.0	40.0	33.0	40.0
56-57	37.663344576996494	40.0	37.0	40.0	33.0	40.0
58-59	37.49338440140848	40.0	37.0	40.0	33.0	40.0
60-61	37.32073181927352	37.0	37.0	40.0	33.0	40.0
62-63	37.17930126468711	37.0	37.0	40.0	33.0	40.0
64-65	37.00304254720754	37.0	37.0	40.0	33.0	40.0
66-67	36.80620567994488	37.0	37.0	40.0	33.0	40.0
68-69	36.60700100180462	37.0	37.0	40.0	33.0	40.0
70-71	36.39660455785686	37.0	37.0	40.0	33.0	40.0
72-73	36.12270377389945	37.0	37.0	37.0	33.0	40.0
74-75	35.794084782160134	37.0	37.0	37.0	33.0	40.0
76-77	33.613182337452	35.0	33.0	37.0	30.0	37.0
78-79	35.29495997667221	37.0	33.0	37.0	33.0	37.0
80-81	35.506248953495515	37.0	33.0	37.0	33.0	37.0
82-83	35.33748303168012	37.0	33.0	37.0	33.0	37.0
84-85	35.190525863152	37.0	33.0	37.0	33.0	37.0
86-87	35.092458913467446	37.0	33.0	37.0	33.0	37.0
88-89	34.9250456854168	37.0	33.0	37.0	33.0	37.0
90-91	34.86631865555911	37.0	33.0	37.0	33.0	37.0
92-93	34.90527719304254	37.0	33.0	37.0	33.0	37.0
94-95	34.890912084642835	37.0	33.0	37.0	33.0	37.0
96-97	34.88638940806028	37.0	33.0	37.0	33.0	37.0
98-99	34.893549449475444	37.0	33.0	37.0	33.0	37.0
100	34.83826010156942	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	3.0
18	7.0
19	20.0
20	53.0
21	187.0
22	650.0
23	1821.0
24	4382.0
25	8991.0
26	16446.0
27	27233.0
28	41890.0
29	60788.0
30	83743.0
31	115858.0
32	159056.0
33	221956.0
34	324005.0
35	509684.0
36	1020052.0
37	2772645.0
38	3155370.0
39	113617.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.44815452017015	20.36959605522189	14.739100427414245	22.443148997193713
2	14.73099624456396	21.097514346099068	42.57890716243995	21.592582246897027
3	17.80432341049757	27.40669688965322	31.077595098569677	23.711384601279534
4	11.978260471949971	18.33080626195092	39.5389779055475	30.151955360551618
5	12.600200174614498	38.24522848869555	35.49386939196787	13.660701944722078
6	28.273298313194324	38.59837021838851	19.18722068220972	13.941110786207444
7	25.10520974923997	33.981817125232304	23.59206932533561	17.320903800192117
8	24.24157181756281	36.14156600634048	21.512346300693945	18.104515875402765
9	25.89693669865617	16.576905276381503	21.988854955363564	35.537303069598764
10-11	23.968723994857562	27.12453023972093	28.73664431450785	20.170101450913666
12-13	25.14054276304841	24.930920345623157	29.284181672298683	20.64435521902975
14-15	22.11018447968376	25.937771532836067	28.183004420464858	23.76903956701532
16-17	20.407617887359063	29.02104171832519	28.660855907385326	21.91048448693042
18-19	20.161007920743256	27.56685019095424	31.855307029947593	20.416834858354914
20-21	21.566962465761527	26.643543597098287	31.69179103825349	20.097702898886695
22-23	21.214647502868168	26.62528602072305	31.518282459668807	20.64178401673998
24-25	20.583171732039673	26.8512907161432	31.81675368720205	20.748783864615078
26-27	20.61469951506002	27.21885096278342	31.5834208573433	20.583028664813263
28-29	20.434070550424877	27.581121414350886	31.44674894168912	20.53805909353512
30-31	20.89894657159383	27.33501168864973	31.27925903485523	20.486782704901213
32-33	20.5781977065308	27.56987613231978	31.209676864334625	20.64224929681479
34-35	20.524976735062808	27.79858856646976	31.13880900863363	20.537625689833803
36-37	20.746687573574523	27.700356246118485	31.028563638495093	20.5243925418119
38-39	20.750644894650865	27.804577162439763	30.91861165071044	20.52616629219893
40-41	20.99570504329829	27.820667641810964	30.581112397323945	20.602514917566804
42-43	21.05276353440605	28.00513712162645	30.07251548485355	20.86958385911395
44-45	21.11553036511206	28.38428038736384	29.526634425223413	20.973554822300688
46-47	21.260739575176967	28.394281095876593	28.799783442539763	21.545195886406677
48-49	21.55158523148599	28.50062250734897	28.558947919810386	21.388844341354655
50-51	21.320679102489542	28.605938643529967	28.357785101958793	21.715597152021697
52-53	21.483580564757006	28.617432471899484	28.097277935122527	21.801709028220984
54-55	21.556030264346244	28.58767607960702	28.129618664202333	21.7266749918444
56-57	21.7046883046424	28.63767606221742	27.929948553243854	21.727687079896327
58-59	21.578362362382737	28.703613614264473	28.1582437703562	21.559780252996593
60-61	21.783403302389683	28.54613664094259	28.005329373138206	21.665130683529526
62-63	21.766469084168342	28.52302694283822	27.840206580223214	21.870297392770226
64-65	21.590099388972533	28.601776687827567	27.985568507337334	21.822555415862567
66-67	21.828883701505735	28.518784609819413	27.70327376598568	21.949057922689175
68-69	21.79542135262642	28.353999290741143	27.872592141550573	21.977987215081864
70-71	21.715951119108514	27.989710913670407	27.94433894080711	22.34999902641396
72-73	21.881247902124596	28.063035714259843	28.096500312843293	21.959216070772268
74-75	21.755665548978943	28.202856545549327	27.983114939738062	22.058362965733668
76-77	21.72781348610161	28.09367375727772	27.779048966563103	22.39946379005757
78-79	21.902566117372537	28.00338044104511	27.83092597331323	22.263127468269122
80-81	21.839449070823623	28.011107875029634	27.957172338381316	22.192270715765428
82-83	22.175492461124655	27.73370451877561	28.060920803002364	22.02988221709737
84-85	22.5406620628754	27.73395969410346	27.730074385292554	21.99530385772859
86-87	21.88106270209007	27.90443014767159	27.929930997699927	22.284576152538417
88-89	22.211046979100665	27.58844765983287	27.837459846724393	22.363045514342065
90-91	22.439229994214273	27.45909552301874	27.89903862826886	22.20263585449813
92-93	22.380013822066356	27.47427779786632	27.838154986648362	22.30755339341896
94-95	22.10369113455146	27.515767292383963	28.02272362286431	22.357817950200268
96-97	22.166469057773252	27.64074209479978	28.050606238793822	22.14218260863314
98-99	23.3526867606361	29.481850360760635	28.132368273656873	19.03309460494639
100	22.57583338970819	30.34881870180566	21.8227112908357	25.252636617650452
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2372.0
1	2620.0
2	5553.0
3	8647.0
4	12873.0
5	23379.5
6	31166.5
7	34536.0
8	34821.0
9	31174.5
10	26219.5
11	21890.5
12	24122.5
13	34739.5
14	59101.0
15	92295.5
16	118529.0
17	131986.5
18	131685.0
19	119980.0
20	102426.0
21	83593.0
22	69888.5
23	65618.5
24	69206.5
25	79988.0
26	97377.5
27	114383.5
28	127016.0
29	141389.0
30	155137.0
31	166339.0
32	182067.5
33	201090.0
34	221802.5
35	242124.0
36	263494.5
37	286686.5
38	300467.0
39	305707.0
40	317552.0
41	328926.0
42	338320.0
43	353324.5
44	367725.5
45	382361.0
46	391647.5
47	394203.5
48	390035.0
49	378761.0
50	361343.5
51	341330.5
52	319731.5
53	298059.0
54	273836.0
55	238812.5
56	202986.5
57	168401.5
58	137153.5
59	114812.0
60	92638.5
61	70625.5
62	52058.0
63	37866.0
64	28138.0
65	20149.5
66	14931.0
67	11912.0
68	9649.5
69	7301.0
70	5302.5
71	3738.5
72	2604.0
73	1926.5
74	1540.0
75	1112.0
76	758.0
77	492.5
78	332.5
79	248.0
80	175.0
81	126.0
82	110.5
83	85.0
84	52.0
85	32.5
86	25.0
87	18.0
88	13.5
89	12.0
90	7.0
91	3.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.440785843955021E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0016495999633267882
12-13	0.009081481903367475
14-15	0.0
16-17	0.0
18-19	1.157614009352132E-5
20-21	5.788291836876211E-6
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.005717773958117306
36-37	0.046398963811459255
38-39	0.15010417934939588
40-41	0.17830072139208775
42-43	0.05508463035313842
44-45	0.004971873925062442
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.014907473280932051
64-65	0.10239176212859641
66-67	0.22177557922977179
68-69	0.24557662395184648
70-71	0.11293714519437931
72-73	0.0074529232733499765
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	165.0
20-21	851.0
22-23	1889.0
24-25	3336.0
26-27	6669.0
28-29	14534.0
30-31	29667.0
32-33	36057.0
34-35	45123.0
36-37	56366.0
38-39	71076.0
40-41	87285.0
42-43	107058.0
44-45	133022.0
46-47	127285.0
48-49	102852.0
50-51	87976.0
52-53	80399.0
54-55	80501.0
56-57	79607.0
58-59	78435.0
60-61	71365.0
62-63	62499.0
64-65	76862.0
66-67	92934.0
68-69	88954.0
70-71	91172.0
72-73	85594.0
74-75	82569.0
76-77	78434.0
78-79	83580.0
80-81	89098.0
82-83	86434.0
84-85	95090.0
86-87	96933.0
88-89	102795.0
90-91	99759.0
92-93	98718.0
94-95	112858.0
96-97	256229.0
98-99	486201.0
100-101	5070227.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.51651861933708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.57579246728798	66.24478236307104
2	7.8835746660622945	12.064473754453793
3	2.4557703366039996	5.63720990056727
4	1.124742981448017	3.442456691277436
5	0.6474963258489319	2.4772082336386076
6	0.39086704607175365	1.7944671365060776
7	0.24120647930106603	1.2919396045181335
8	0.15721501827487874	0.9623636698455343
9	0.11553565164679118	0.7956347256386047
>10	0.40065057322458025	4.74076031666894
>50	0.005583220857541365	0.2890228103601375
>100	0.0014260447793314369	0.18377130469376676
>500	1.3918859276124842E-4	0.0759094887606488
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1576140093521321E-5
2	0.0	0.0	0.0	0.0	1.1576140093521321E-5
3	0.0	0.0	0.0	0.0	1.1576140093521321E-5
4	0.0	0.0	0.0	0.0	2.3152280187042643E-5
5	0.0	0.0	0.0	0.0	3.472842028056396E-5
6	0.0	0.0	0.0	0.0	6.945684056112792E-5
7	0.0	0.0	0.0	0.0	6.945684056112792E-5
8	0.0	0.0	0.0	0.0	8.103298065464925E-5
9	0.0	0.0	0.0	1.1576140093521321E-5	1.0418526084169188E-4
10-11	1.1576140093521321E-5	0.0	0.0	1.1576140093521321E-5	1.157614009352132E-4
12-13	2.3152280187042643E-5	0.0	0.0	1.1576140093521321E-5	1.2154947098197386E-4
14-15	2.89403502338033E-5	0.0	0.0	1.1576140093521321E-5	1.9100631154310178E-4
16-17	4.0516490327324624E-5	0.0	0.0	1.1576140093521321E-5	2.315228018704264E-4
18-19	4.6304560374085285E-5	0.0	0.0	1.736421014028198E-5	2.89403502338033E-4
20-21	4.6304560374085285E-5	0.0	0.0	2.3152280187042643E-5	2.89403502338033E-4
22-23	4.6304560374085285E-5	0.0	0.0	2.89403502338033E-5	3.5307227285240024E-4
24-25	5.209263042084594E-5	0.0	0.0	9.839719079493122E-5	4.2831718346028884E-4
26-27	5.78807004676066E-5	0.0	0.0	2.778273622445117E-4	4.4568139360057083E-4
28-29	6.945684056112792E-5	0.0	0.0	9.434554176219877E-4	4.5725753369409215E-4
30-31	6.945684056112792E-5	0.0	0.0	0.0028361543229127235	4.630456037408528E-4
32-33	6.945684056112792E-5	0.0	0.0	0.005215051112131355	4.8619788392789546E-4
34-35	7.524491060788859E-5	0.0	0.0	0.008132238415698727	5.093501641149381E-4
36-37	8.103298065464925E-5	0.0	0.0	0.011755570264970901	5.209263042084594E-4
38-39	8.103298065464925E-5	0.0	0.0	0.016791191205652676	5.498666544422627E-4
40-41	1.0418526084169188E-4	0.0	0.0	0.022145155998906284	5.730189346293054E-4
42-43	1.0418526084169188E-4	0.0	0.0	0.027672762893562717	5.903831447695874E-4
44-45	1.0418526084169188E-4	0.0	0.0	0.03399912345467212	6.077473549098694E-4
46-47	1.0418526084169188E-4	0.0	0.0	0.04082325803980294	6.424757751904333E-4
48-49	1.0418526084169188E-4	0.0	0.0	0.04723065158156699	7.061445457048006E-4
50-51	1.0418526084169188E-4	0.0	0.0	0.05320972793987075	7.061445457048006E-4
52-53	1.0418526084169188E-4	0.0	0.0	0.05913092359770691	7.582371761256465E-4
54-55	1.0418526084169188E-4	0.0	0.0	0.06436333891997854	8.161178765932531E-4
56-57	1.0418526084169188E-4	0.0	0.0	0.06895327846705974	8.219059466400137E-4
58-59	1.0418526084169188E-4	0.0	0.0	0.07369370783535673	8.276940166867744E-4
60-61	1.0418526084169188E-4	0.0	0.0	0.07865408386543062	8.450582268270564E-4
62-63	1.0418526084169188E-4	0.0	0.0	0.08280413008895801	8.50846296873817E-4
64-65	1.0418526084169188E-4	0.0	0.0	0.08691944789220483	8.624224369673383E-4
66-67	1.0418526084169188E-4	0.0	0.0	0.09216922742461675	9.02938927294663E-4
68-69	1.0418526084169188E-4	0.0	0.0	0.0981656679930608	9.37667347575227E-4
70-71	1.0418526084169188E-4	0.0	0.0	0.10287715701112399	9.492434876687483E-4
72-73	1.0418526084169188E-4	0.0	0.0	0.10725293796647503	0.0010302764683233975
74-75	1.0418526084169188E-4	0.0	0.0	0.11051740947284805	0.0010302764683233975
76-77	1.0418526084169188E-4	0.0	0.0	0.11377030483912753	0.001036064538370158
78-79	1.3891368112225585E-4	0.0	0.0	0.11587716233614842	0.0010418526084169189
80-81	1.3891368112225585E-4	0.0	0.0	0.11658909495189998	0.0010534287485104402
82-83	1.3891368112225585E-4	0.0	0.0	0.1172026303768566	0.001093945238837765
84-85	1.3891368112225585E-4	0.0	0.0	0.11759043106998956	0.0010997333088845255
86-87	1.3891368112225585E-4	0.0	0.0	0.11780458966171972	0.0011113094489780468
88	1.3891368112225585E-4	0.0	0.0	0.11782195387186	0.0011228855890715681
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25320	0.0	42.668488	1
GTATCAA	43540	0.0	34.310287	1
ATCAACG	55020	0.0	26.852715	3
TATCAAC	55300	0.0	26.828285	2
AACGCAG	55440	0.0	26.728765	6
CAACGCA	56125	0.0	26.332417	5
TCAACGC	56610	0.0	26.134773	4
ACGCAGA	61760	0.0	23.921524	7
CGCAGAG	61940	0.0	23.832115	8
GCAGAGT	67475	0.0	21.761501	9
TACATGG	39195	0.0	20.324562	2
GTACATG	40275	0.0	20.207762	1
GAGTACT	45665	0.0	20.091051	12-13
ACATGGG	39515	0.0	19.65066	3
ACGGTAC	1145	0.0	19.007195	3
CAGAGTA	66935	0.0	18.970192	10-11
AGAGTAC	65060	0.0	18.715141	10-11
AGTACTT	48255	0.0	18.433977	12-13
GTACTAG	2445	0.0	18.306181	1
CATGGGG	25345	0.0	18.291496	4
>>END_MODULE