FastQCFastQC Report
Fri 27 May 2016
SRR2139134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9993177
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA288350.047.5616151
GTATCAA530900.036.1376761
ATCAACG686500.027.7037013
TATCAAC689250.027.6876682
TCAACGC694200.027.4081444
AACGCAG697250.027.2931676
CAACGCA700400.027.154855
ACGCAGA775450.024.5211457
CGCAGAG781350.024.3359858
GCAGAGT841500.022.4029149
GAGTACT583700.020.22038712-13
GTACATG489500.020.133421
TACATGG481050.019.953962
ACATGGG482100.019.4545353
CAGAGTA842600.019.29306210-11
AGAGTAC823400.019.13222710-11
AGTACTT620250.018.65141712-13
CATGGGG309100.018.319084
GTACTTT649650.018.19761314-15
GGTTCAC44150.017.7061546
Produced by FastQC (version 0.11.5)