##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139134_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9993177
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68396286786474	33.0	33.0	33.0	33.0	33.0
2	32.61657709054888	33.0	33.0	33.0	33.0	33.0
3	32.68611533649409	33.0	33.0	33.0	33.0	33.0
4	36.65888555761596	37.0	37.0	37.0	37.0	37.0
5	36.667619616864584	37.0	37.0	37.0	37.0	37.0
6	36.659637470646224	37.0	37.0	37.0	37.0	37.0
7	36.6426683926443	37.0	37.0	37.0	37.0	37.0
8	36.63761324351605	37.0	37.0	37.0	37.0	37.0
9	36.63698761665084	37.0	37.0	37.0	37.0	37.0
10-11	36.64106609939962	37.0	37.0	37.0	37.0	37.0
12-13	36.63898097672042	37.0	37.0	37.0	37.0	37.0
14-15	38.7819249073643	40.0	37.0	40.0	37.0	40.0
16-17	38.716649920240584	40.0	37.0	40.0	37.0	40.0
18-19	38.78137223027271	40.0	37.0	40.0	37.0	40.0
20-21	38.806498720237514	40.0	37.0	40.0	37.0	40.0
22-23	38.81275516996959	40.0	37.0	40.0	37.0	40.0
24-25	38.80120578479149	40.0	37.0	40.0	37.0	40.0
26-27	38.708977564830676	40.0	37.0	40.0	37.0	40.0
28-29	38.598001616007565	40.0	37.0	40.0	37.0	40.0
30-31	38.419572706561276	40.0	37.0	40.0	37.0	40.0
32-33	38.301409201482514	40.0	37.0	40.0	37.0	40.0
34-35	38.264619907707356	40.0	37.0	40.0	37.0	40.0
36-37	38.23383099128594	40.0	37.0	40.0	37.0	40.0
38-39	38.193903106977956	40.0	37.0	40.0	37.0	40.0
40-41	38.16564711057143	40.0	37.0	40.0	37.0	40.0
42-43	38.13169261866123	40.0	37.0	40.0	37.0	40.0
44-45	38.032793771047864	40.0	37.0	40.0	35.0	40.0
46-47	37.6501012208702	40.0	37.0	40.0	33.0	40.0
48-49	37.82427415027199	40.0	37.0	40.0	33.0	40.0
50-51	37.84267096786924	40.0	37.0	40.0	33.0	40.0
52-53	37.81357048054455	40.0	37.0	40.0	33.0	40.0
54-55	37.722891065735865	40.0	37.0	40.0	33.0	40.0
56-57	37.556689564503515	40.0	37.0	40.0	33.0	40.0
58-59	37.39245954218868	38.5	37.0	40.0	33.0	40.0
60-61	37.21750432912597	37.0	37.0	40.0	33.0	40.0
62-63	37.085798410319406	37.0	37.0	40.0	33.0	40.0
64-65	36.91607031729849	37.0	37.0	40.0	33.0	40.0
66-67	36.71999630560114	37.0	37.0	40.0	33.0	40.0
68-69	36.525634598199254	37.0	37.0	40.0	33.0	40.0
70-71	36.3132667125267	37.0	37.0	40.0	33.0	40.0
72-73	36.04228825857036	37.0	37.0	37.0	33.0	40.0
74-75	35.71108594798483	37.0	35.0	37.0	33.0	40.0
76-77	33.51916694659451	35.0	33.0	37.0	27.0	37.0
78-79	35.2273426957963	37.0	33.0	37.0	33.0	37.0
80-81	35.45231908178768	37.0	33.0	37.0	33.0	37.0
82-83	35.28071340579043	37.0	33.0	37.0	33.0	37.0
84-85	35.12660370859819	37.0	33.0	37.0	33.0	37.0
86-87	35.02563323108035	37.0	33.0	37.0	33.0	37.0
88-89	34.84939108921232	37.0	33.0	37.0	33.0	37.0
90-91	34.78751837753424	37.0	33.0	37.0	33.0	37.0
92-93	34.82521175897388	37.0	33.0	37.0	33.0	37.0
94-95	34.80949180160603	37.0	33.0	37.0	33.0	37.0
96-97	34.80386253627992	37.0	33.0	37.0	33.0	37.0
98-99	34.809687186237504	37.0	33.0	37.0	33.0	37.0
100	34.75524541983266	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	6.0
19	21.0
20	46.0
21	241.0
22	832.0
23	2393.0
24	5952.0
25	11843.0
26	21604.0
27	35779.0
28	55157.0
29	78430.0
30	109232.0
31	150189.0
32	204696.0
33	281280.0
34	404155.0
35	625062.0
36	1220795.0
37	3191528.0
38	3472210.0
39	121720.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.44906573226885	20.63199339869395	14.952116314596346	21.966824554440862
2	14.670554539190238	21.217875167390062	42.80960732407457	21.30196296934513
3	17.644992707522885	27.913400896111597	31.464994858991265	22.976611537374257
4	11.83474721497422	18.34459018279976	39.81531395177208	30.005348650453932
5	12.312493276661321	38.49928576253293	35.65053148774038	13.537689473065367
6	27.97319170333753	38.86529556422258	19.18372289087302	13.97778984156687
7	24.709534257130493	34.40240964458243	23.81818591188486	17.06987018640222
8	24.12110265256037	36.37167366727792	21.46980314064349	18.03742053951822
9	25.85905880763621	16.549945337692975	22.175574829821354	35.41542102484946
10-11	23.857219775205834	27.26034406955623	28.827774938848492	20.054661216389437
12-13	25.068489072196932	25.19103858851139	29.425304265915088	20.31516807337659
14-15	22.030048304963305	26.180245399460595	28.36470107201154	23.42500522356456
16-17	20.1215009122225	29.275987934166274	28.920375264080207	21.682135889531015
18-19	19.952100313253766	27.572695607482544	32.323632646918256	20.151571432345435
20-21	21.227449659049825	26.65372169126819	32.36000007605469	19.758828573627298
22-23	20.90449910569154	26.543286361162906	32.22404921614021	20.328165317005354
24-25	20.321089620374817	26.795566033958035	32.45551517699824	20.42782916866891
26-27	20.30419522246806	27.08973575760781	32.24614697637297	20.35992204355116
28-29	20.23094406626925	27.41756126683827	32.02225412871754	20.32924053817494
30-31	20.623301705110052	27.25389218096553	31.84089324501048	20.28191286891393
32-33	20.269951739098875	27.52935429701693	31.812895779666743	20.387798184217452
34-35	20.36693635174615	27.694592520656748	31.63752403123079	20.30094709636631
36-37	20.521461304769502	27.641388411637546	31.48332439965093	20.353825883942022
38-39	20.520905325584383	27.775446078569576	31.3152214681582	20.38842712768784
40-41	20.781469802479783	27.7777355240711	31.075662219191997	20.36513245425712
42-43	20.81607556438906	27.959496784937667	30.54246569399817	20.681961956675103
44-45	20.875576022098056	28.419134071259773	29.863662574378928	20.84162733226324
46-47	21.11502317508971	28.318081318710338	29.265834826188318	21.301060680011634
48-49	21.280833951336653	28.56569706360137	28.86131784793587	21.29215113712611
50-51	21.1317260532454	28.670668402786028	28.717041819877377	21.480563724091194
52-53	21.35892282424273	28.698297686278977	28.422536938955652	21.520242550522646
54-55	21.343636620281348	28.726320949323046	28.401442257571013	21.52860017282459
56-57	21.454466603634962	28.84167917940763	28.22822939072408	21.47562482623333
58-59	21.43217580557325	28.729630459233864	28.406502965644663	21.431690769548222
60-61	21.447030944008304	28.657305851455284	28.40898061337102	21.48668259116539
62-63	21.47868822223054	28.66949122842481	28.246177757210088	21.605642792134557
64-65	21.45687565281696	28.685869621786747	28.251321973259163	21.605932752137125
66-67	21.53517845192664	28.746253087757367	27.974532953512966	21.74403550680303
68-69	21.631609416014776	28.545894313942526	28.17633349855709	21.64616277148561
70-71	21.53295168806017	28.18695756581698	28.139746566885375	22.140344179237477
72-73	21.699976238693917	28.259272390550716	28.28492748353152	21.755823887223848
74-75	21.57930637295645	28.44031253561699	28.169953228032856	21.810427863393702
76-77	21.54538821480237	28.287325619777846	28.03624650868156	22.131039656738224
78-79	21.777204339431147	28.23284460986826	28.058704596442833	21.931246454257757
80-81	21.708369478725263	28.09708183993815	28.164360210359668	22.030188470976924
82-83	21.925681344438676	27.924505218693884	28.24866124415028	21.901152192717166
84-85	22.21089321786635	27.87202570407567	28.095749110984247	21.82133196707373
86-87	21.766454199138856	28.051707571788466	28.129300711085747	22.05253751798693
88-89	22.055549915965386	27.852317114107976	28.021752583581204	22.070380386345434
90-91	22.19140776364491	27.71432577317331	28.11470170124664	21.979564761935144
92-93	22.19213051051826	27.74868638401953	27.980542103333516	22.078641002128688
94-95	21.896123988258168	27.743147803548634	28.156899071369857	22.20382913682334
96-97	22.09239775647484	27.798310578705156	28.245133162664104	21.864158502155906
98-99	23.207681040970673	29.701285606771684	28.35665281882653	18.734380533431118
100	22.28192510880678	30.428022380734927	22.26477014600241	25.025282364455887
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3353.0
1	3579.5
2	7057.5
3	10787.5
4	15068.5
5	26740.5
6	36232.5
7	41367.0
8	43631.0
9	40422.0
10	34268.5
11	28795.0
12	31163.5
13	42965.5
14	70999.5
15	110467.0
16	142004.5
17	158738.5
18	160042.5
19	148313.0
20	130267.0
21	109871.0
22	94369.5
23	89540.0
24	93830.5
25	107517.5
26	126734.0
27	145906.0
28	163539.5
29	182335.0
30	199634.0
31	215152.5
32	236173.0
33	257921.0
34	278331.0
35	295531.0
36	317472.0
37	345328.0
38	357045.5
39	355880.5
40	362669.5
41	371033.5
42	375617.0
43	384399.0
44	397791.5
45	414141.0
46	422773.5
47	422309.0
48	419428.0
49	413155.5
50	399080.0
51	382246.0
52	360304.5
53	335445.0
54	311135.5
55	278673.5
56	242683.0
57	203911.5
58	168709.0
59	140961.5
60	112329.5
61	86688.5
62	65432.0
63	47379.0
64	34608.0
65	25662.0
66	19341.0
67	14929.5
68	11322.5
69	8348.0
70	6243.0
71	4422.0
72	3330.0
73	2513.5
74	1800.5
75	1278.5
76	876.5
77	622.5
78	408.5
79	249.5
80	173.0
81	125.5
82	90.0
83	56.0
84	41.0
85	32.5
86	22.5
87	16.0
88	9.0
89	4.5
90	3.0
91	2.0
92	1.5
93	0.5
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.0020482975534204E-5
2	5.103482105840815E-4
3	2.0013655317022807E-5
4	2.0013655317022807E-5
5	2.0013655317022807E-5
6	2.0013655317022807E-5
7	2.0013655317022807E-5
8	2.0013655317022807E-5
9	2.0013655317022807E-5
10-11	0.0016861504604591712
12-13	0.008901073202245893
14-15	1.0006827658511404E-5
16-17	1.0006827658511404E-5
18-19	1.0006827658511404E-5
20-21	1.0007195173329624E-5
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.0027181684109283E-5
30-31	1.5075014781051993E-5
32-33	1.0088296300122376E-5
34-35	0.005874716078621442
36-37	0.04736299244324578
38-39	0.15323091041341314
40-41	0.18080719014727434
42-43	0.05571711302533927
44-45	0.005218083581094386
46-47	1.0866675508518306E-5
48-49	5.517886477321128E-6
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.015391500208448988
64-65	0.10491542837141694
66-67	0.22582732129447086
68-69	0.2496828003631795
70-71	0.11572633347855853
72-73	0.007466555233890002
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	205.0
20-21	964.0
22-23	2372.0
24-25	4382.0
26-27	8641.0
28-29	17914.0
30-31	35568.0
32-33	45332.0
34-35	58912.0
36-37	79033.0
38-39	98667.0
40-41	107826.0
42-43	132690.0
44-45	159946.0
46-47	146732.0
48-49	123506.0
50-51	102361.0
52-53	91325.0
54-55	97883.0
56-57	98617.0
58-59	96498.0
60-61	98702.0
62-63	91324.0
64-65	93508.0
66-67	105371.0
68-69	99739.0
70-71	102622.0
72-73	99333.0
74-75	96217.0
76-77	92699.0
78-79	96340.0
80-81	100837.0
82-83	103615.0
84-85	103893.0
86-87	107972.0
88-89	118326.0
90-91	116750.0
92-93	113871.0
94-95	131298.0
96-97	292331.0
98-99	542301.0
100-101	5776754.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.90613615486961
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.23390983891886	67.08822947393513
2	7.615103618705005	11.712963914671345
3	2.28396063112901	5.269517618099087
4	1.0523040590425603	3.2371455696419638
5	0.5751397685131213	2.2115888672675146
6	0.35270666609204027	1.6275184131122746
7	0.2252939484049708	1.2128540949630597
8	0.1466294902070909	0.9021366030548521
9	0.10276906460699893	0.7113214507657005
>10	0.4028782940282826	4.695282308326213
>50	0.006462913467535265	0.3285489780799864
>100	0.002203113892834726	0.29919545748389376
>500	2.554372179209646E-4	0.14411834886597266
>1k	3.8315577378270337E-4	0.5595789017329889
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0013655317022804E-5
2	0.0	0.0	0.0	0.0	2.0013655317022804E-5
3	0.0	0.0	0.0	0.0	2.0013655317022804E-5
4	0.0	0.0	0.0	2.0013655317022804E-5	3.0020482975534207E-5
5	0.0	0.0	0.0	2.0013655317022804E-5	3.0020482975534207E-5
6	0.0	0.0	0.0	2.0013655317022804E-5	5.0034138292557014E-5
7	0.0	0.0	0.0	2.0013655317022804E-5	6.0040965951068415E-5
8	0.0	0.0	0.0	2.0013655317022804E-5	6.0040965951068415E-5
9	0.0	0.0	0.0	3.0020482975534207E-5	8.005462126809121E-5
10-11	5.0034138292557014E-5	0.0	0.0	3.0020482975534207E-5	8.005462126809121E-5
12-13	5.0034138292557014E-5	0.0	0.0	3.0020482975534207E-5	8.005462126809121E-5
14-15	5.0034138292557014E-5	0.0	0.0	3.0020482975534207E-5	1.0507169041436973E-4
16-17	5.0034138292557014E-5	0.0	0.0	3.502389680478991E-5	1.1007510424362543E-4
18-19	5.0034138292557014E-5	0.0	0.0	5.0034138292557014E-5	1.1007510424362543E-4
20-21	5.0034138292557014E-5	0.0	0.0	5.5037552121812715E-5	1.1507851807288113E-4
22-23	6.0040965951068415E-5	0.0	0.0	7.505120743883551E-5	1.3008875956064823E-4
24-25	6.0040965951068415E-5	0.0	0.0	8.505803509734692E-5	1.4009558721915963E-4
26-27	6.0040965951068415E-5	0.0	0.0	1.8512631168246096E-4	1.5010241487767103E-4
28-29	6.0040965951068415E-5	0.0	0.0	5.303618659011043E-4	1.6010924253618243E-4
30-31	6.0040965951068415E-5	0.0	0.0	0.0013409149062405278	1.6010924253618243E-4
32-33	6.0040965951068415E-5	0.0	0.0	0.003062089263504489	1.7011607019469383E-4
34-35	6.0040965951068415E-5	0.0	0.0	0.004998410415426445	1.8012289785320523E-4
36-37	6.0040965951068415E-5	0.0	0.0	0.007925407505541032	1.8012289785320523E-4
38-39	6.0040965951068415E-5	0.0	0.0	0.012863776955016407	1.8012289785320523E-4
40-41	6.0040965951068415E-5	0.0	0.0	0.018777812101196646	1.8012289785320523E-4
42-43	6.0040965951068415E-5	0.0	0.0	0.024331601451670473	1.8512631168246096E-4
44-45	6.0040965951068415E-5	0.0	0.0	0.030700947256312984	1.9012972551171666E-4
46-47	6.0040965951068415E-5	0.0	0.0	0.03792587682575821	2.1014338082873946E-4
48-49	6.0040965951068415E-5	0.0	0.0	0.0450657483601061	2.1014338082873946E-4
50-51	6.0040965951068415E-5	0.0	0.0	0.052100548204039615	2.1014338082873946E-4
52-53	6.0040965951068415E-5	0.0	0.0	0.059190385600094944	2.1514679465799516E-4
54-55	6.0040965951068415E-5	0.0	0.0	0.06540962898985977	2.3015703614576226E-4
56-57	6.0040965951068415E-5	0.0	0.0	0.07186403282959963	2.3015703614576226E-4
58-59	6.0040965951068415E-5	0.0	0.0	0.07824838887572991	2.3015703614576226E-4
60-61	6.0040965951068415E-5	0.0	0.0	0.08506804192500544	2.3015703614576226E-4
62-63	6.0040965951068415E-5	0.0	0.0	0.09169756524876924	2.3015703614576226E-4
64-65	6.0040965951068415E-5	0.0	0.0	0.09765663111941278	2.3015703614576226E-4
66-67	6.0040965951068415E-5	0.0	0.0	0.10393591547512868	2.4016386380427366E-4
68-69	6.0040965951068415E-5	0.0	0.0	0.11026023055530788	2.5017069146278506E-4
70-71	6.0040965951068415E-5	0.0	0.0	0.11540373997178274	2.5517410529204076E-4
72-73	6.0040965951068415E-5	0.0	0.0	0.11939146079369954	3.3022531273087626E-4
74-75	6.0040965951068415E-5	0.0	0.0	0.12272373440398385	3.3022531273087626E-4
76-77	6.0040965951068415E-5	0.0	0.0	0.12613606263553623	3.3022531273087626E-4
78-79	6.0040965951068415E-5	0.0	0.0	0.12819746913318958	3.3022531273087626E-4
80-81	6.0040965951068415E-5	0.0	0.0	0.12901802900118753	3.4523555421864336E-4
82-83	6.0040965951068415E-5	0.0	0.0	0.1295634011085764	3.7025262336492186E-4
84-85	6.0040965951068415E-5	0.0	0.0	0.12993365373194132	3.7025262336492186E-4
86-87	6.0040965951068415E-5	0.0	0.0	0.13007374931916046	3.7525603719417756E-4
88	6.0040965951068415E-5	0.0	0.0	0.13009876638830675	3.802594510234333E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	28835	0.0	47.561615	1
GTATCAA	53090	0.0	36.137676	1
ATCAACG	68650	0.0	27.703701	3
TATCAAC	68925	0.0	27.687668	2
TCAACGC	69420	0.0	27.408144	4
AACGCAG	69725	0.0	27.293167	6
CAACGCA	70040	0.0	27.15485	5
ACGCAGA	77545	0.0	24.521145	7
CGCAGAG	78135	0.0	24.335985	8
GCAGAGT	84150	0.0	22.402914	9
GAGTACT	58370	0.0	20.220387	12-13
GTACATG	48950	0.0	20.13342	1
TACATGG	48105	0.0	19.95396	2
ACATGGG	48210	0.0	19.454535	3
CAGAGTA	84260	0.0	19.293062	10-11
AGAGTAC	82340	0.0	19.132227	10-11
AGTACTT	62025	0.0	18.651417	12-13
CATGGGG	30910	0.0	18.31908	4
GTACTTT	64965	0.0	18.197613	14-15
GGTTCAC	4415	0.0	17.706154	6
>>END_MODULE