FastQCFastQC Report
Fri 27 May 2016
SRR2139133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8249358
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA226750.048.885421
GTATCAA410300.036.2832871
TATCAAC523150.028.2986982
ATCAACG524800.028.2174193
AACGCAG529400.028.023446
TCAACGC531600.027.8564744
CAACGCA531750.027.8418665
ACGCAGA588650.025.1333857
CGCAGAG591050.025.0103728
GCAGAGT646700.022.8070959
TACATGG352450.021.587892
GTACATG361400.021.464511
GAGTACT456250.021.20257812-13
ACATGGG361350.020.6676083
CAGAGTA637500.020.06284310-11
AGAGTAC622300.019.16610710-11
GTACTTT506650.019.10476314-15
AGTACTT475850.019.08416412-13
CATGGGG227350.018.757944
GTACTGG58150.018.5299031
Produced by FastQC (version 0.11.5)