##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139133_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8249358
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73352035394754	33.0	33.0	33.0	33.0	33.0
2	32.663762198221974	33.0	33.0	33.0	33.0	33.0
3	32.73825235854717	33.0	33.0	33.0	33.0	33.0
4	36.72586351568207	37.0	37.0	37.0	37.0	37.0
5	36.73078086803846	37.0	37.0	37.0	37.0	37.0
6	36.72719986209836	37.0	37.0	37.0	37.0	37.0
7	36.71524002231446	37.0	37.0	37.0	37.0	37.0
8	36.71089313859333	37.0	37.0	37.0	37.0	37.0
9	36.70149410414726	37.0	37.0	37.0	37.0	37.0
10-11	36.7112849872681	37.0	37.0	37.0	37.0	37.0
12-13	36.70962910083427	37.0	37.0	37.0	37.0	37.0
14-15	38.94595700659373	40.0	40.0	40.0	37.0	40.0
16-17	38.885154032592595	40.0	37.0	40.0	37.0	40.0
18-19	38.94472363788794	40.0	38.5	40.0	37.0	40.0
20-21	38.96306216040166	40.0	40.0	40.0	37.0	40.0
22-23	38.967281676617674	40.0	40.0	40.0	37.0	40.0
24-25	38.95885053589008	40.0	40.0	40.0	37.0	40.0
26-27	38.88037206721465	40.0	38.5	40.0	37.0	40.0
28-29	38.78146180482521	40.0	37.0	40.0	37.0	40.0
30-31	38.609988167821356	40.0	37.0	40.0	37.0	40.0
32-33	38.503568156257586	40.0	37.0	40.0	37.0	40.0
34-35	38.474115556567426	40.0	37.0	40.0	37.0	40.0
36-37	38.441874510212436	40.0	37.0	40.0	37.0	40.0
38-39	38.39895727539701	40.0	37.0	40.0	37.0	40.0
40-41	38.36779119037358	40.0	37.0	40.0	37.0	40.0
42-43	38.33115260099244	40.0	37.0	40.0	37.0	40.0
44-45	38.23172748709875	40.0	37.0	40.0	37.0	40.0
46-47	37.839971896706416	40.0	37.0	40.0	33.0	40.0
48-49	38.01612591957405	40.0	37.0	40.0	35.0	40.0
50-51	38.02492370118826	40.0	37.0	40.0	37.0	40.0
52-53	37.98609189336821	40.0	37.0	40.0	35.0	40.0
54-55	37.89046868470999	40.0	37.0	40.0	33.0	40.0
56-57	37.715521460860714	40.0	37.0	40.0	33.0	40.0
58-59	37.539057034753945	40.0	37.0	40.0	33.0	40.0
60-61	37.36033385018416	37.0	37.0	40.0	33.0	40.0
62-63	37.213800279772414	37.0	37.0	40.0	33.0	40.0
64-65	37.03089691265014	37.0	37.0	40.0	33.0	40.0
66-67	36.82782183960746	37.0	37.0	40.0	33.0	40.0
68-69	36.62433328626574	37.0	37.0	40.0	33.0	40.0
70-71	36.4040838906638	37.0	37.0	38.5	33.0	40.0
72-73	36.12636001570037	37.0	37.0	37.0	33.0	40.0
74-75	35.79783174253354	37.0	37.0	37.0	33.0	40.0
76-77	33.64153656300827	35.0	33.0	37.0	30.0	37.0
78-79	35.29241009867336	37.0	33.0	37.0	33.0	37.0
80-81	35.50301396262167	37.0	33.0	37.0	33.0	37.0
82-83	35.34192864419343	37.0	33.0	37.0	33.0	37.0
84-85	35.199292747210635	37.0	33.0	37.0	33.0	37.0
86-87	35.109278508117	37.0	33.0	37.0	33.0	37.0
88-89	34.94010375389233	37.0	33.0	37.0	33.0	37.0
90-91	34.88768077773791	37.0	33.0	37.0	33.0	37.0
92-93	34.94055903806445	37.0	33.0	37.0	33.0	37.0
94-95	34.9313478070315	37.0	33.0	37.0	33.0	37.0
96-97	34.93138293318843	37.0	33.0	37.0	33.0	37.0
98-99	34.936499731945695	37.0	33.0	37.0	33.0	37.0
100	34.87942103629627	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	0.0
17	2.0
18	4.0
19	17.0
20	36.0
21	148.0
22	580.0
23	1541.0
24	3794.0
25	8002.0
26	14856.0
27	24873.0
28	37536.0
29	54700.0
30	76875.0
31	105714.0
32	144620.0
33	201232.0
34	292029.0
35	462335.0
36	932570.0
37	2680347.0
38	3086779.0
39	120766.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8510402147853	20.10271349732355	14.560897738174408	22.48534854971674
2	14.946757436157473	20.94675428438292	42.281261694144284	21.825226585315324
3	17.84977128514896	27.211242575934726	31.217931583742004	23.721054555174316
4	12.15250161982361	18.119598441332684	39.07569743331448	30.652202505529218
5	12.626647780341616	38.07696480512719	35.47096712409637	13.825420290434828
6	28.701565152669513	38.16700820852055	18.924776543135795	14.206650095674147
7	25.14043825244519	33.981468854231636	23.490600174195436	17.387492719127735
8	24.223124299263567	36.082872883304724	21.38632138238168	18.30768143505003
9	25.93579111681068	16.2879967493224	21.869922710339726	35.90628942352719
10-11	24.11434867430888	26.768173569749067	28.74411058347235	20.373367172469706
12-13	25.173942173422027	24.810664392030862	29.284483217943695	20.730910216603416
14-15	22.173198205242155	25.848563003327047	28.113018006977025	23.865220784453772
16-17	20.418970785362934	28.89589711102367	28.636089014442096	22.049043089171303
18-19	20.32428462917963	27.287414366063395	31.81197251955849	20.576328485198484
20-21	21.53080454848629	26.340889898672266	31.93324487087884	20.195060681962605
22-23	21.272752082627747	26.328792671492664	31.675509735579645	20.722945510299947
24-25	20.695491206391207	26.56467786275834	31.937783232055956	20.802047698794492
26-27	20.69578368413138	26.87035828224119	31.83505706955077	20.59880096407666
28-29	20.52451897266689	27.12936356888031	31.677373163501144	20.66874429495165
30-31	20.84433833300096	26.977611672431834	31.528032771466425	20.650017223100782
32-33	20.505895559458963	27.189189311225824	31.470362700819678	20.83455242849553
34-35	20.612381947413976	27.356208631694546	31.296168535082575	20.735240885808906
36-37	20.851387742036028	27.3703308049009	31.07166942188218	20.70661203118089
38-39	20.77961026181695	27.430067695440318	30.936139308074072	20.85418273466866
40-41	20.990048248830668	27.455716676764098	30.748041317555085	20.806193756850153
42-43	21.13558520498884	27.68408010391314	30.139790912644866	21.04054377845316
44-45	21.189558212862583	28.052853474710382	29.554325996517754	21.203262315909278
46-47	21.37156060262107	28.05193700783535	28.976232899094683	21.600269490448902
48-49	21.633989513205933	28.181982128759476	28.510440955598497	21.67358740243609
50-51	21.44175715954163	28.253616365393107	28.404577068660597	21.900049406404662
52-53	21.668395646516377	28.309364100009628	28.05351254515534	21.96872770831866
54-55	21.712053896628444	28.34430885967195	28.064347407006174	21.879289836693427
56-57	21.794759847056948	28.311478629232933	27.8849978292873	22.00876369442282
58-59	21.648804353098363	28.376171559786755	28.089709896331705	21.885314190783177
60-61	21.842773556525643	28.183944646276704	28.06691266406679	21.906369133130863
62-63	21.827310993780365	28.242443622588816	27.935836374761326	21.9944090088695
64-65	21.756732923546043	28.301640116804	27.942664812460055	21.998962147189904
66-67	21.94436607490478	28.236008875389185	27.667613335671405	22.152011714034632
68-69	21.93388855651672	28.16301749182908	27.710903236244448	22.192190715409758
70-71	21.856487267147887	27.79362807312287	27.7785192497765	22.57136540995274
72-73	22.048716800241117	27.89775425092063	27.890578187398113	22.16295076144014
74-75	21.960425642795784	28.023323790215983	27.785042791643473	22.231207775344764
76-77	21.971277420993005	27.816683140947244	27.59026489136889	22.62177454669087
78-79	22.03746080995322	27.785157687211115	27.67788943717135	22.499492065664317
80-81	22.052268267581205	27.692479225816964	27.752964252629564	22.50228825397227
82-83	22.297719094764055	27.496292677344602	27.814850781416705	22.391137446474637
84-85	22.57626125965735	27.412474092328072	27.69037886616775	22.32088578184683
86-87	22.129719301173036	27.548215711502017	27.79469101687033	22.52737397045461
88-89	22.368087750554423	27.31177466092746	27.69924637237149	22.620891216146628
90-91	22.545591207386366	27.131931411245674	27.754691113439883	22.567786267928078
92-93	22.478472248128327	27.219709257349262	27.77029638740793	22.53152210711448
94-95	22.354975589252344	27.139132155156005	27.905639630869356	22.600252624722298
96-97	22.455854374491405	27.394497656975087	27.833544802182164	22.316103166351343
98-99	23.64523303536648	29.171924228324396	27.919328894545526	19.263513841763597
100	22.73663584317653	29.759428166267764	21.621763778784704	25.88217221177101
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2437.0
1	2647.5
2	5454.0
3	8518.5
4	12265.5
5	21919.5
6	29257.5
7	32777.5
8	33857.5
9	30915.5
10	26526.5
11	22793.5
12	25342.5
13	35322.5
14	58369.5
15	90979.5
16	116954.5
17	130510.0
18	131213.0
19	119970.5
20	101991.5
21	82128.5
22	66012.0
23	58568.0
24	58864.0
25	66052.0
26	77740.5
27	90098.5
28	103388.5
29	119616.5
30	135345.5
31	149923.5
32	167715.5
33	185391.0
34	203384.5
35	219779.5
36	237027.5
37	256517.0
38	269963.5
39	279775.5
40	293947.5
41	307462.5
42	319357.0
43	335432.0
44	351628.0
45	367220.5
46	377457.5
47	379633.0
48	375287.0
49	362165.5
50	346744.0
51	331343.0
52	311439.0
53	289494.0
54	262446.0
55	228998.5
56	196598.0
57	163054.0
58	133640.0
59	111625.5
60	89053.0
61	69078.0
62	52359.0
63	38238.0
64	28126.0
65	21030.0
66	15832.0
67	12031.5
68	8944.5
69	6440.0
70	4489.5
71	3158.0
72	2234.5
73	1494.5
74	1021.0
75	647.0
76	422.5
77	278.0
78	167.5
79	98.5
80	69.5
81	48.0
82	33.5
83	22.0
84	13.5
85	9.5
86	8.0
87	7.5
88	5.5
89	3.5
90	2.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.848862178123436E-5
2	4.4851975147641796E-4
3	3.636646633592578E-5
4	3.636646633592578E-5
5	3.636646633592578E-5
6	3.636646633592578E-5
7	3.636646633592578E-5
8	1.212215544530859E-5
9	1.212215544530859E-5
10-11	0.0016061855965033886
12-13	0.008624913599337064
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.0358103582699448E-5
30-31	4.2586243073879894E-5
32-33	3.661097791155972E-5
34-35	0.0056081170269534925
36-37	0.04575404605058638
38-39	0.14901554112943444
40-41	0.17581701549211912
42-43	0.05274213407064346
44-45	0.004499842505512307
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.014533011035865516
64-65	0.10002643898609487
66-67	0.21868233436009085
68-69	0.24208140954400767
70-71	0.1104105430799253
72-73	0.00705596456231493
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	135.0
20-21	726.0
22-23	1595.0
24-25	3046.0
26-27	6121.0
28-29	13128.0
30-31	24087.0
32-33	26237.0
34-35	33371.0
36-37	48351.0
38-39	62604.0
40-41	77026.0
42-43	83580.0
44-45	104500.0
46-47	106689.0
48-49	87483.0
50-51	79964.0
52-53	65188.0
54-55	70640.0
56-57	77970.0
58-59	71869.0
60-61	34183.0
62-63	64590.0
64-65	85310.0
66-67	106187.0
68-69	100188.0
70-71	101845.0
72-73	95108.0
74-75	89798.0
76-77	88249.0
78-79	93280.0
80-81	92929.0
82-83	93077.0
84-85	99682.0
86-87	106038.0
88-89	110624.0
90-91	107507.0
92-93	106921.0
94-95	115884.0
96-97	255737.0
98-99	466868.0
100-101	4791043.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.30002387558412
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.45919391212034	65.9683855975854
2	7.891531031005409	12.042480121612515
3	2.578483065553074	5.9021495839346665
4	1.1709273380068357	3.5736713538598286
5	0.619648200440946	2.363958624405346
6	0.36598874598080344	1.6754970033918233
7	0.24492603504151955	1.3081503624994064
8	0.15777276551846134	0.9630452620780429
9	0.11744328494195648	0.8064832900588478
>10	0.38729803556712045	4.44480241707848
>50	0.005151337604785909	0.26231920748527743
>100	0.0011096925880889213	0.16076874984504066
>500	1.880555836128947E-4	0.10069879407840099
>1k	3.385000470222806E-4	0.42758963208702866
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	3.030538861327148E-5	0.0
20-21	0.0	0.0	0.0	3.636646633592578E-5	0.0
22-23	0.0	0.0	0.0	6.667185494919727E-5	0.0
24-25	0.0	0.0	0.0	1.454658653437031E-4	0.0
26-27	0.0	0.0	0.0	3.8790897424987496E-4	0.0
28-29	0.0	0.0	0.0	9.697724356246874E-4	0.0
30-31	0.0	0.0	0.0	0.0022547209128273983	0.0
32-33	0.0	0.0	0.0	0.004788251400896894	0.0
34-35	0.0	0.0	0.0	0.0072126824899586125	0.0
36-37	0.0	0.0	0.0	0.01045535907157866	0.0
38-39	0.0	0.0	0.0	0.016061855965033886	0.0
40-41	0.0	0.0	0.0	0.02269873607134034	0.0
42-43	0.0	0.0	0.0	0.028953768281119573	6.061077722654296E-6
44-45	0.0	0.0	0.0	0.03476634181714504	2.4244310890617185E-5
46-47	0.0	0.0	0.0	0.04106986264870551	3.636646633592578E-5
48-49	0.0	0.0	0.0	0.048391644537671905	3.636646633592578E-5
50-51	0.0	0.0	0.0	0.05655591623008724	3.636646633592578E-5
52-53	0.0	0.0	0.0	0.06502324180863528	4.2427544058580074E-5
54-55	0.0	0.0	0.0	0.07207227520008223	6.061077722654296E-5
56-57	0.0	0.0	0.0	0.0771575194093892	7.273293267185156E-5
58-59	0.0	0.0	0.0	0.08215184745285634	7.273293267185156E-5
60-61	0.0	0.0	0.0	0.08597032641812855	7.273293267185156E-5
62-63	0.0	0.0	0.0	0.08869781139332297	7.273293267185156E-5
64-65	0.0	0.0	0.0	0.0928193442447279	7.273293267185156E-5
66-67	0.0	0.0	0.0	0.0993956135738078	8.485508811716015E-5
68-69	0.0	0.0	0.0	0.10729319784642635	8.485508811716015E-5
70-71	0.0	0.0	0.0	0.11383310070917034	8.485508811716015E-5
72-73	0.0	0.0	0.0	0.11937292574767637	8.485508811716015E-5
74-75	0.0	0.0	0.0	0.12369447416392887	8.485508811716015E-5
76-77	0.0	0.0	0.0	0.12743415911880657	8.485508811716015E-5
78-79	0.0	0.0	0.0	0.12980404050836442	8.485508811716015E-5
80-81	0.0	0.0	0.0	0.13059198061230948	8.485508811716015E-5
82-83	0.0	0.0	0.0	0.13122233269546552	8.485508811716015E-5
84-85	0.0	0.0	0.0	0.131634485980606	9.091616583981445E-5
86-87	1.2122155445308593E-5	0.0	0.0	0.13175570753505908	9.697724356246874E-5
88	1.2122155445308593E-5	0.0	0.0	0.13175570753505908	9.697724356246874E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	22675	0.0	48.88542	1
GTATCAA	41030	0.0	36.283287	1
TATCAAC	52315	0.0	28.298698	2
ATCAACG	52480	0.0	28.217419	3
AACGCAG	52940	0.0	28.02344	6
TCAACGC	53160	0.0	27.856474	4
CAACGCA	53175	0.0	27.841866	5
ACGCAGA	58865	0.0	25.133385	7
CGCAGAG	59105	0.0	25.010372	8
GCAGAGT	64670	0.0	22.807095	9
TACATGG	35245	0.0	21.58789	2
GTACATG	36140	0.0	21.46451	1
GAGTACT	45625	0.0	21.202578	12-13
ACATGGG	36135	0.0	20.667608	3
CAGAGTA	63750	0.0	20.062843	10-11
AGAGTAC	62230	0.0	19.166107	10-11
GTACTTT	50665	0.0	19.104763	14-15
AGTACTT	47585	0.0	19.084164	12-13
CATGGGG	22735	0.0	18.75794	4
GTACTGG	5815	0.0	18.529903	1
>>END_MODULE