FastQCFastQC Report
Fri 27 May 2016
SRR2139132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10454685
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA246200.049.5046351
CTTATAC123100.048.3394281
TATACAC157350.040.109473
GTATCAA465200.036.4362561
TTATACA176600.034.809162
TATCAAC582900.028.8680742
ATCAACG583100.028.7035483
TCAACGC586450.028.6234444
CAACGCA589100.028.4459885
AACGCAG594200.028.2529536
TACACAT232400.027.3859795
ACACATC228400.026.3430776
ATACACA262100.025.111434
ACGCAGA673200.024.895217
CGCAGAG677700.024.8085258
ACATCTC248600.024.2853538
CACATCT254900.023.4439327
GCAGAGT747600.022.4176759
CATCTCC284400.021.1706899
GTACATG462250.020.8256741
Produced by FastQC (version 0.11.5)