FastQCFastQC Report
Fri 27 May 2016
SRR2139131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12536034
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA367700.040.363971
GTATCAA628500.033.0833171
TATCAAC789350.026.220972
ATCAACG798400.025.7295933
AACGCAG805100.025.5091656
TCAACGC811550.025.3518434
CAACGCA814950.025.2265765
ACGGTAC18950.023.8987143
GGTTCAC80950.023.6084656
GTACTGG100700.023.1179221
ACGCAGA892450.022.9191977
CGCAGAG897450.022.8579148
TACTGGT90800.022.7508772
GCAGAGT993550.020.5070429
GTACATG594350.020.3060821
TACATGG587050.020.2066972
TAGCGCG4058.4944095E-619.93541794
CTCACGA14800.019.77546194
GAGTACT650550.019.44494812-13
ACATGGG596550.019.3787173
Produced by FastQC (version 0.11.5)