##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139131_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12536034
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65924589866301	33.0	33.0	33.0	33.0	33.0
2	32.66050578675839	33.0	33.0	33.0	33.0	33.0
3	32.6248786498186	33.0	33.0	33.0	33.0	33.0
4	36.56896846323167	37.0	37.0	37.0	37.0	37.0
5	36.60067083417292	37.0	37.0	37.0	37.0	37.0
6	36.582709172613924	37.0	37.0	37.0	37.0	37.0
7	36.486599111010705	37.0	37.0	37.0	37.0	37.0
8	36.526578820701985	37.0	37.0	37.0	37.0	37.0
9	36.57679103295349	37.0	37.0	37.0	37.0	37.0
10-11	36.5627440065973	37.0	37.0	37.0	37.0	37.0
12-13	36.546827449574565	37.0	37.0	37.0	37.0	37.0
14-15	38.594664269417265	40.0	37.0	40.0	37.0	40.0
16-17	38.53142185957696	40.0	37.0	40.0	37.0	40.0
18-19	38.5828600576546	40.0	37.0	40.0	37.0	40.0
20-21	38.604615759729946	40.0	37.0	40.0	37.0	40.0
22-23	38.58763525347668	40.0	37.0	40.0	37.0	40.0
24-25	38.6012789422295	40.0	37.0	40.0	37.0	40.0
26-27	38.51655909145131	40.0	37.0	40.0	37.0	40.0
28-29	38.39477657812997	40.0	37.0	40.0	37.0	40.0
30-31	38.20916929520345	40.0	37.0	40.0	37.0	40.0
32-33	38.10725648696465	40.0	37.0	40.0	37.0	40.0
34-35	38.07610051988268	40.0	37.0	40.0	37.0	40.0
36-37	38.051965520588745	40.0	37.0	40.0	35.0	40.0
38-39	38.02333824272769	40.0	37.0	40.0	33.0	40.0
40-41	38.001488641512424	40.0	37.0	40.0	33.0	40.0
42-43	37.958673443560436	40.0	37.0	40.0	33.0	40.0
44-45	37.85252296961252	40.0	37.0	40.0	33.0	40.0
46-47	37.764993509689106	40.0	37.0	40.0	33.0	40.0
48-49	37.711680135750306	40.0	37.0	40.0	33.0	40.0
50-51	37.66715138173492	40.0	37.0	40.0	33.0	40.0
52-53	37.61021326302148	40.0	37.0	40.0	33.0	40.0
54-55	37.5127865232605	40.0	37.0	40.0	33.0	40.0
56-57	37.337341601401135	38.5	37.0	40.0	33.0	40.0
58-59	37.128577143076555	37.0	37.0	40.0	33.0	40.0
60-61	36.96489516143459	37.0	37.0	40.0	33.0	40.0
62-63	36.82916498262399	37.0	37.0	40.0	33.0	40.0
64-65	36.67050228274449	37.0	37.0	40.0	33.0	40.0
66-67	36.478216451950885	37.0	37.0	40.0	33.0	40.0
68-69	36.28478494843188	37.0	37.0	40.0	33.0	40.0
70-71	36.0883859917697	37.0	37.0	37.0	33.0	40.0
72-73	35.80614940853266	37.0	37.0	37.0	33.0	40.0
74-75	35.47405159247408	37.0	33.0	37.0	33.0	40.0
76-77	33.27008319571	35.0	33.0	37.0	27.0	37.0
78-79	34.989004296558086	37.0	33.0	37.0	33.0	37.0
80-81	35.23759357726185	37.0	33.0	37.0	33.0	37.0
82-83	35.124969691553545	37.0	33.0	37.0	33.0	37.0
84-85	34.96755732359529	37.0	33.0	37.0	33.0	37.0
86-87	34.86231529929345	37.0	33.0	37.0	33.0	37.0
88-89	34.77395304850457	37.0	33.0	37.0	33.0	37.0
90-91	34.69957310762143	37.0	33.0	37.0	33.0	37.0
92-93	34.68877627860429	37.0	33.0	37.0	33.0	37.0
94-95	34.65503678995311	37.0	33.0	37.0	33.0	37.0
96-97	34.663827768295945	37.0	33.0	37.0	33.0	37.0
98-99	34.6841442055715	37.0	33.0	37.0	33.0	37.0
100	34.53979678178746	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	1.0
17	1.0
18	11.0
19	32.0
20	117.0
21	364.0
22	1280.0
23	3627.0
24	8505.0
25	16813.0
26	30863.0
27	50904.0
28	77368.0
29	112963.0
30	157984.0
31	216806.0
32	296461.0
33	408114.0
34	575597.0
35	861544.0
36	1575525.0
37	3869422.0
38	4117901.0
39	153828.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.45483061070192	20.336511531477978	14.555273222775241	22.653384635044862
2	14.834205223684146	20.86677706888492	42.54151649890954	21.757501208521393
3	18.335870818474167	27.126585649017866	30.615264763959637	23.92227876854833
4	12.239532853851545	18.04804454104065	38.739421096018084	30.973001509089716
5	12.785104124637826	37.86933730396711	35.35477807415008	13.99078049724498
6	28.896651046096395	37.88036950123141	18.884672776094895	14.338306676577298
7	25.486058828493924	33.497292684432736	23.290531917829835	17.726116569243512
8	24.123530615823157	36.16261729985735	21.406195930866172	18.307656153453316
9	25.63822018989419	16.35994286550276	21.88815059052967	36.11368635407339
10-11	24.370059940807433	26.792975354087268	28.39215736013479	20.444807344970506
12-13	25.356472344983256	24.57915290341737	29.02035261680035	21.044022134799032
14-15	22.304418606394975	25.669454151129457	27.888226052992515	24.137901189483056
16-17	20.693543962432134	28.678330696800174	28.310658563199382	22.31746677756831
18-19	20.43210160662468	27.213581042742945	31.46160352321983	20.89271382741255
20-21	21.81062385283117	26.288302231414264	31.392869224279035	20.50820469147553
22-23	21.50696345616152	26.400122166107533	31.059468254304807	21.033446123426145
24-25	20.806920738872954	26.64251029415814	31.45948197809047	21.091086988878438
26-27	20.94311303786833	26.917208101318668	31.276907116528935	20.862771744284068
28-29	20.71412948433908	27.26493108900336	31.085885608752843	20.935053817904723
30-31	21.117551125373893	27.16818959476074	30.886502785891963	20.827756493973407
32-33	20.805904151718924	27.232742829612043	30.906194392488757	21.055158626180273
34-35	20.77390848586984	27.464779838368088	30.785436158761453	20.975875517000624
36-37	21.038448491729504	27.474401873880478	30.514989464665792	20.97216016972422
38-39	21.025313963237107	27.45856032019854	30.466881899131348	21.049243817433005
40-41	21.260256908339432	27.49938236149914	30.22107798715471	21.019282743006716
42-43	21.426726634177744	27.706653731777525	29.663552905957964	21.203066728086768
44-45	21.416496285984092	28.158292480896435	29.008693400870243	21.416517832249227
46-47	21.54366332271754	28.049607271810928	28.476413727465598	21.930315678005936
48-49	21.854371196738686	28.144324939777388	28.019261396001678	21.98204246748225
50-51	21.57249671710357	28.275123479324314	27.952138426551365	22.200241377020753
52-53	21.826884263791637	28.290733067021257	27.70367281448966	22.17870985469744
54-55	21.84326710919678	28.33545629823227	27.640933062884105	22.180343529686844
56-57	21.998976602827796	28.34311317570951	27.444485049444673	22.213425172018024
58-59	21.85789178891088	28.458243786363084	27.695174093960368	21.98869033076567
60-61	22.06377944804655	28.345319293448263	27.45816754101748	22.13273371748771
62-63	22.02382281511974	28.328917784423695	27.361189080942506	22.286070319514057
64-65	21.905617892344576	28.441095911591685	27.43613038795226	22.217155808111478
66-67	22.106373645532855	28.249625137065397	27.245669088208157	22.398332129193594
68-69	22.14494247393726	28.09375987965951	27.340880282230245	22.420417364172987
70-71	22.025625460895917	27.775666030951758	27.384247801431606	22.814460706720723
72-73	22.280612988433663	27.884991008723613	27.5257525548899	22.30864344795282
74-75	22.15744042588016	27.883714752594003	27.405753353350132	22.553091468175708
76-77	22.153659020156645	27.73707790325076	27.280433259046855	22.828829817545742
78-79	22.291247253425873	27.66799824360598	27.302933081950865	22.737821421017287
80-81	22.133845669695297	27.64858440247897	27.420229311405446	22.79734061642029
82-83	22.535500680788175	27.386473717659143	27.5143471112137	22.56367849033898
84-85	22.846183741950586	27.370791607341687	27.287367568892044	22.495657081815686
86-87	22.29159698831898	27.531387166839462	27.40350448315832	22.77351136168324
88-89	22.62543136979914	27.245478333519124	27.434783598897706	22.69430669778403
90-91	22.810820050759606	27.083226470951644	27.362757063509175	22.743196414779575
92-93	22.780112131276177	27.150692817245627	27.35269722273249	22.71649782874571
94-95	22.469027811420872	27.130573062339224	27.59051781143487	22.80988131480503
96-97	22.593884360166825	27.2921911302433	27.49648575167613	22.617438757913742
98-99	23.939709020252742	29.09855762651948	27.550209961530687	19.41152339169709
100	31.078623639133006	40.22631975459372	28.695056606273273	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3023.0
1	3346.5
2	7707.5
3	12269.0
4	18234.5
5	34725.0
6	47350.0
7	51505.5
8	50279.0
9	43942.0
10	37196.5
11	30740.0
12	32701.0
13	46852.0
14	81247.0
15	130974.5
16	172722.5
17	196507.5
18	199998.0
19	185123.0
20	159470.0
21	130752.5
22	107528.5
23	96875.0
24	98766.5
25	111969.5
26	132159.5
27	151794.5
28	171195.0
29	194693.5
30	216666.5
31	235822.5
32	262338.5
33	295406.0
34	325572.5
35	353710.5
36	384040.0
37	412224.0
38	430727.0
39	441516.0
40	456653.0
41	473881.5
42	491881.5
43	516302.5
44	551142.0
45	584519.5
46	597934.5
47	604903.5
48	606308.5
49	596408.5
50	580388.5
51	554968.5
52	521382.0
53	490945.5
54	450851.0
55	395432.5
56	338272.0
57	279058.0
58	225262.0
59	179062.0
60	141317.0
61	110451.5
62	83381.0
63	62120.0
64	45860.5
65	33887.5
66	26336.0
67	20749.5
68	15672.0
69	11289.5
70	7934.5
71	5461.5
72	3917.5
73	3132.0
74	2421.5
75	1666.0
76	1087.0
77	710.5
78	427.5
79	239.0
80	169.0
81	129.5
82	98.5
83	63.0
84	38.0
85	28.0
86	21.5
87	15.0
88	7.5
89	3.5
90	3.5
91	4.0
92	2.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.307582445931464E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.006700683804782278
14-15	0.0
16-17	7.977004529502712E-6
18-19	7.977004529502712E-6
20-21	0.0
22-23	0.0
24-25	1.5961479204546916E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0018215318175867847
36-37	0.03643407636903836
38-39	0.11727833450581432
40-41	0.15341375142119756
42-43	0.05523823931172044
44-45	0.0016891986519160557
46-47	8.786454450361145E-6
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.01006197309882805
64-65	0.07748896288140127
66-67	0.18280763385657076
68-69	0.20133319737490646
70-71	0.08154303502081811
72-73	0.005818401859561235
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	292.0
20-21	1339.0
22-23	3051.0
24-25	6205.0
26-27	11499.0
28-29	23008.0
30-31	52461.0
32-33	66166.0
34-35	86966.0
36-37	120629.0
38-39	152538.0
40-41	160915.0
42-43	192443.0
44-45	222578.0
46-47	211113.0
48-49	177207.0
50-51	149432.0
52-53	137352.0
54-55	140833.0
56-57	141416.0
58-59	140251.0
60-61	136837.0
62-63	134746.0
64-65	140891.0
66-67	157708.0
68-69	139124.0
70-71	142058.0
72-73	137193.0
74-75	133535.0
76-77	127168.0
78-79	133226.0
80-81	140983.0
82-83	133555.0
84-85	142385.0
86-87	156962.0
88-89	163016.0
90-91	160819.0
92-93	157303.0
94-95	174717.0
96-97	371104.0
98-99	2418543.0
100-101	4936467.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.13083611223036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.58200544830706	67.55273308613837
2	7.465358611977652	11.516187032389515
3	2.1775654869134233	5.0387234008430495
4	0.9965088898551161	3.074462554711823
5	0.5115487975708088	1.972809323442128
6	0.3405709358500171	1.5761112622581952
7	0.2214953455925468	1.1958884840364385
8	0.15064822763969807	0.9295699005340419
9	0.10926989054702327	0.758527021780542
>10	0.43123376342942926	5.3252942699988015
>50	0.009692620164480957	0.496610789534278
>100	0.003969686578815729	0.48430753017977574
>500	1.0583653642741472E-4	0.054673405327166115
>1k	2.645903757124657E-5	0.024101938825928898
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.977004529502712E-6
2	0.0	0.0	0.0	2.3931013588508135E-5	7.977004529502712E-6
3	0.0	0.0	0.0	2.3931013588508135E-5	7.977004529502712E-6
4	0.0	0.0	0.0	3.190801811801085E-5	7.977004529502712E-6
5	0.0	0.0	0.0	3.988502264751356E-5	7.977004529502712E-6
6	0.0	0.0	0.0	3.988502264751356E-5	7.977004529502712E-6
7	0.0	0.0	0.0	7.17930407655244E-5	7.977004529502712E-6
8	0.0	0.0	0.0	7.17930407655244E-5	7.977004529502712E-6
9	3.190801811801085E-5	0.0	0.0	1.1167806341303796E-4	1.5954009059005424E-5
10-11	3.190801811801085E-5	0.0	0.0	1.356090770015461E-4	1.5954009059005424E-5
12-13	3.190801811801085E-5	0.0	0.0	2.2335612682607592E-4	1.5954009059005424E-5
14-15	3.190801811801085E-5	0.0	0.0	2.3532163362033E-4	2.791951585325949E-5
16-17	3.190801811801085E-5	0.0	0.0	3.111031766506058E-4	3.190801811801085E-5
18-19	3.190801811801085E-5	0.0	0.0	4.0283872873988694E-4	3.190801811801085E-5
20-21	3.988502264751356E-5	0.0	0.0	4.3075824459314646E-4	3.988502264751356E-5
22-23	3.988502264751356E-5	0.0	0.0	5.224937966824277E-4	3.988502264751356E-5
24-25	3.988502264751356E-5	0.0	0.0	6.341718600954655E-4	5.185052944176763E-5
26-27	3.988502264751356E-5	0.0	0.0	8.814590005100497E-4	6.38160362360217E-5
28-29	3.988502264751356E-5	0.0	0.0	0.001523607865135018	6.38160362360217E-5
30-31	3.988502264751356E-5	0.0	0.0	0.0036813875903655017	6.38160362360217E-5
32-33	3.988502264751356E-5	0.0	0.0	0.008846498023218508	6.38160362360217E-5
34-35	3.988502264751356E-5	0.0	0.0	0.01520815913549692	6.38160362360217E-5
36-37	3.988502264751356E-5	0.0	0.0	0.023771473497918083	6.38160362360217E-5
38-39	3.988502264751356E-5	0.0	0.0	0.03675803687194849	6.38160362360217E-5
40-41	3.988502264751356E-5	0.0	0.0	0.04999587588865825	6.38160362360217E-5
42-43	3.988502264751356E-5	0.0	0.0	0.06246792247053574	6.38160362360217E-5
44-45	3.988502264751356E-5	0.0	0.0	0.0760407956774846	6.38160362360217E-5
46-47	3.988502264751356E-5	0.0	0.0	0.0892826231964591	6.38160362360217E-5
48-49	3.988502264751356E-5	0.0	0.0	0.10140368157903848	6.38160362360217E-5
50-51	3.988502264751356E-5	0.0	0.0	0.11239200531842847	6.38160362360217E-5
52-53	3.988502264751356E-5	0.0	0.0	0.12296552482228432	7.17930407655244E-5
54-55	3.988502264751356E-5	3.988502264751356E-6	0.0	0.13190774689985685	7.17930407655244E-5
56-57	3.988502264751356E-5	7.977004529502712E-6	0.0	0.13865629273181612	7.17930407655244E-5
58-59	3.988502264751356E-5	7.977004529502712E-6	0.0	0.14564016019739576	7.17930407655244E-5
60-61	3.988502264751356E-5	7.977004529502712E-6	0.0	0.15235679801123705	7.17930407655244E-5
62-63	3.988502264751356E-5	7.977004529502712E-6	0.0	0.15946430904702397	7.17930407655244E-5
64-65	3.988502264751356E-5	7.977004529502712E-6	0.0	0.16624077439483653	7.17930407655244E-5
66-67	3.988502264751356E-5	7.977004529502712E-6	0.0	0.17311695229926785	7.977004529502712E-5
68-69	3.988502264751356E-5	7.977004529502712E-6	0.0	0.18028429086902603	7.977004529502712E-5
70-71	3.988502264751356E-5	7.977004529502712E-6	0.0	0.186123458184622	7.977004529502712E-5
72-73	3.988502264751356E-5	7.977004529502712E-6	0.0	0.1908418563638229	7.977004529502712E-5
74-75	3.988502264751356E-5	7.977004529502712E-6	0.0	0.19465486452892516	7.977004529502712E-5
76-77	3.988502264751356E-5	7.977004529502712E-6	0.0	0.1984399531781742	7.977004529502712E-5
78-79	3.988502264751356E-5	7.977004529502712E-6	0.0	0.20070941096681771	7.977004529502712E-5
80-81	3.988502264751356E-5	7.977004529502712E-6	0.0	0.20147919190391475	7.977004529502712E-5
82-83	3.988502264751356E-5	7.977004529502712E-6	0.0	0.20216521429345197	7.977004529502712E-5
84-85	3.988502264751356E-5	7.977004529502712E-6	0.0	0.2025560875153976	7.977004529502712E-5
86-87	3.988502264751356E-5	7.977004529502712E-6	0.0	0.20276747813542945	7.977004529502712E-5
88	3.988502264751356E-5	7.977004529502712E-6	0.0	0.20278343214448843	7.977004529502712E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	36770	0.0	40.36397	1
GTATCAA	62850	0.0	33.083317	1
TATCAAC	78935	0.0	26.22097	2
ATCAACG	79840	0.0	25.729593	3
AACGCAG	80510	0.0	25.509165	6
TCAACGC	81155	0.0	25.351843	4
CAACGCA	81495	0.0	25.226576	5
ACGGTAC	1895	0.0	23.898714	3
GGTTCAC	8095	0.0	23.608465	6
GTACTGG	10070	0.0	23.117922	1
ACGCAGA	89245	0.0	22.919197	7
CGCAGAG	89745	0.0	22.857914	8
TACTGGT	9080	0.0	22.750877	2
GCAGAGT	99355	0.0	20.507042	9
GTACATG	59435	0.0	20.306082	1
TACATGG	58705	0.0	20.206697	2
TAGCGCG	405	8.4944095E-6	19.935417	94
CTCACGA	1480	0.0	19.775461	94
GAGTACT	65055	0.0	19.444948	12-13
ACATGGG	59655	0.0	19.378717	3
>>END_MODULE