FastQCFastQC Report
Fri 27 May 2016
SRR2139130_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139130_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8612248
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145700.052.0153241
CTTATAC102500.040.853811
GTATCAA277000.038.39631
TATACAC131750.034.156013
ATCAACG345250.030.2229543
TCAACGC348700.029.9976024
TATCAAC354450.029.7165012
CAACGCA354500.029.506815
TTATACA150700.029.2645282
AACGCAG359850.029.1434066
ACGCAGA402350.026.0545147
CGCAGAG404600.025.8778698
GCAGAGT462750.022.5982489
TACACAT207550.021.7643035
ATACACA227800.020.7317924
ACATGGG255350.020.6077733
GAGTACT330600.020.4385812-13
GTACATG277150.020.3000931
ACACATC209850.020.1818226
TACATGG269950.020.0643332
Produced by FastQC (version 0.11.5)