##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139130_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8612248
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68275762611574	33.0	33.0	33.0	33.0	33.0
2	32.696964950382295	33.0	33.0	33.0	33.0	33.0
3	32.66504471306446	33.0	33.0	33.0	33.0	33.0
4	36.61128116607882	37.0	37.0	37.0	37.0	37.0
5	36.63879837180722	37.0	37.0	37.0	37.0	37.0
6	36.61266942150296	37.0	37.0	37.0	37.0	37.0
7	36.512034836897406	37.0	37.0	37.0	37.0	37.0
8	36.551585137817675	37.0	37.0	37.0	37.0	37.0
9	36.60452416140362	37.0	37.0	37.0	37.0	37.0
10-11	36.5875430549608	37.0	37.0	37.0	37.0	37.0
12-13	36.57347529936435	37.0	37.0	37.0	37.0	37.0
14-15	38.68504715609676	40.0	37.0	40.0	37.0	40.0
16-17	38.621029027496654	40.0	37.0	40.0	37.0	40.0
18-19	38.65572676262922	40.0	37.0	40.0	37.0	40.0
20-21	38.67042884191983	40.0	37.0	40.0	37.0	40.0
22-23	38.64799534897976	40.0	37.0	40.0	37.0	40.0
24-25	38.66963175217833	40.0	37.0	40.0	37.0	40.0
26-27	38.59255250218304	40.0	37.0	40.0	37.0	40.0
28-29	38.471915075418785	40.0	37.0	40.0	37.0	40.0
30-31	38.32746597691944	40.0	37.0	40.0	37.0	40.0
32-33	38.22366400690332	40.0	37.0	40.0	37.0	40.0
34-35	38.177203727993245	40.0	37.0	40.0	37.0	40.0
36-37	38.15537140181701	40.0	37.0	40.0	37.0	40.0
38-39	38.10893946020343	40.0	37.0	40.0	37.0	40.0
40-41	38.10155142873245	40.0	37.0	40.0	37.0	40.0
42-43	38.07275203845642	40.0	37.0	40.0	37.0	40.0
44-45	37.97982484604563	40.0	37.0	40.0	33.0	40.0
46-47	37.900106841851915	40.0	37.0	40.0	33.0	40.0
48-49	37.847587852132634	40.0	37.0	40.0	33.0	40.0
50-51	37.77895299476765	40.0	37.0	40.0	33.0	40.0
52-53	37.72009486219666	40.0	37.0	40.0	33.0	40.0
54-55	37.63634404432095	40.0	37.0	40.0	33.0	40.0
56-57	37.47502590767091	40.0	37.0	40.0	33.0	40.0
58-59	37.27319595710776	38.5	37.0	40.0	33.0	40.0
60-61	37.12095735235636	37.0	37.0	40.0	33.0	40.0
62-63	36.99047485342183	37.0	37.0	40.0	33.0	40.0
64-65	36.85171410247483	37.0	37.0	40.0	33.0	40.0
66-67	36.66221818069638	37.0	37.0	40.0	33.0	40.0
68-69	36.46735912543021	37.0	37.0	40.0	33.0	40.0
70-71	36.25846917181623	37.0	37.0	40.0	33.0	40.0
72-73	35.96684796128383	37.0	37.0	37.0	33.0	40.0
74-75	35.62777416810782	37.0	35.0	37.0	33.0	40.0
76-77	33.49022778324398	35.0	33.0	37.0	27.0	37.0
78-79	35.11705085273259	37.0	33.0	37.0	33.0	37.0
80-81	35.32101272427022	37.0	33.0	37.0	33.0	37.0
82-83	35.18317948689965	37.0	33.0	37.0	33.0	37.0
84-85	35.010833108942464	37.0	33.0	37.0	33.0	37.0
86-87	34.89561319838377	37.0	33.0	37.0	33.0	37.0
88-89	34.79785763844329	37.0	33.0	37.0	33.0	37.0
90-91	34.712093818400334	37.0	33.0	37.0	33.0	37.0
92-93	34.695741953836816	37.0	33.0	37.0	33.0	37.0
94-95	34.653820151061325	37.0	33.0	37.0	33.0	37.0
96-97	34.65919382102433	37.0	33.0	37.0	33.0	37.0
98-99	34.67824614569875	37.0	33.0	37.0	33.0	37.0
100	34.63271422590914	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	4.0
18	2.0
19	18.0
20	52.0
21	264.0
22	909.0
23	2698.0
24	6287.0
25	13156.0
26	23460.0
27	37990.0
28	56408.0
29	80282.0
30	109496.0
31	147041.0
32	198321.0
33	268479.0
34	377910.0
35	564973.0
36	1026697.0
37	2569526.0
38	3041257.0
39	87017.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.275800296461576	21.51984511357432	15.191635259838144	23.012719330125964
2	14.132954460480072	20.88180133048281	43.54500330460978	21.44024090442734
3	17.66330176820309	27.176569271244688	31.414801667300264	23.745327293251957
4	11.799523608591764	18.2661874730471	39.36456297230711	30.569725946054028
5	12.481262146947497	38.23538017841106	36.154715041813716	13.12864263282772
6	28.510201809121362	38.64942563769943	18.92745296320461	13.912919589974601
7	25.12477578444095	33.581708283365735	23.374675229974798	17.918840702218514
8	23.478666661712484	37.45140641560717	21.696298109390256	17.373628813290097
9	24.713942283129793	17.260835962921643	21.641341494113963	36.3838802598346
10-11	23.621387818836613	27.139505272026536	29.21431779484288	20.024789114293966
12-13	24.55197545701235	24.514015320265283	29.50838603752085	21.425623185201516
14-15	21.638113533191333	26.09051666881864	27.37286478512927	24.898505012860753
16-17	20.752200819112502	29.40591120924525	27.996807569870256	21.845080401771988
18-19	20.596579429668072	27.862928471172683	30.412408003113704	21.128084096045537
20-21	21.481544313555688	27.184619451381938	30.469226124113387	20.86461011094899
22-23	21.17670427763563	27.380203220701716	30.360021780863107	21.08307072079955
24-25	20.944157295938577	27.79994765180237	30.46989156884482	20.786003483414234
26-27	20.966731950306976	27.93573779992486	30.41811757828968	20.67941267147848
28-29	20.73696943656797	27.753203333583855	30.453551801081915	21.056275428766266
30-31	21.54459245815048	27.647421238029914	30.38385816289779	20.42412814092182
32-33	21.266355990315855	27.741106539597236	30.50876751053081	20.4837699595561
34-35	20.927495906940745	27.849612957417747	30.683679858851924	20.539211276789583
36-37	21.072547331726636	27.49450999647732	30.5148431973363	20.918099474459748
38-39	20.845222078701493	27.52397063132477	30.5524680540756	21.07833923589815
40-41	20.98900270475696	27.53566161004139	30.600063443091457	20.875272242110192
42-43	21.178166674197026	27.476779427523628	30.35300684898608	20.992047049293262
44-45	20.956091793411083	27.935406627135546	29.866878056645348	21.241623522808023
46-47	21.40128989883984	27.571001274501374	29.282826830810095	21.744881995848697
48-49	21.327737370683245	27.667543345937677	29.334940662820426	21.66977862055865
50-51	21.00809555226942	27.95777474595483	29.43923442550256	21.594895276273192
52-53	21.24969224079325	28.121499047630717	29.382687090037173	21.246121621538858
54-55	21.204585663232766	28.203525779363137	29.0074834672524	21.5844050901517
56-57	21.47145459098369	28.55091233480428	28.4951098020617	21.48252327215033
58-59	21.031414773390022	28.918109315793572	29.035072330250866	21.01540358056554
60-61	21.42556874319442	29.197701020149296	28.348242767995345	21.028487468660934
62-63	21.120371030120406	29.29005327801706	28.371991112668194	21.21758457919434
64-65	21.19077302395505	29.023837029069664	28.483881408313007	21.30150853866228
66-67	21.39650234683955	28.858778409716514	28.27579483102618	21.468924412417753
68-69	21.38814701346609	28.53669918849529	28.58575083459329	21.489402963445333
70-71	21.468913739199134	28.12004048702183	28.6370676241387	21.77397814964033
72-73	21.491712356412982	28.233581814949638	28.667976616503854	21.606729212133523
74-75	21.388913428089136	28.374990662786935	28.587193539023755	21.64890237010018
76-77	21.385462158475303	28.310106276283886	28.37716590650363	21.92726565873718
78-79	21.5223087798245	28.258826923554654	28.42808012399051	21.79078417263033
80-81	21.584880622616204	28.152658313046032	28.47677937505775	21.785681689280022
82-83	21.70091977348047	28.05978811995275	28.49835405364056	21.740938052926218
84-85	21.978638363400663	28.03026789166686	28.30038953809508	21.6907042068374
86-87	21.48413179490087	28.168923267329433	28.468050244773664	21.87889469299603
88-89	21.730496319996366	27.97766700882679	28.46215128394122	21.829685387235624
90-91	21.86396192633815	27.902574281723254	28.461251848953328	21.772211942985276
92-93	21.9828887365587	27.97470009898595	28.244863960544674	21.79754720391068
94-95	21.833786489093406	27.96073127774823	28.35980729149692	21.845674941661443
96-97	21.87309625245442	27.9881975804427	28.55032195310227	21.588384214000612
98-99	22.979106644497254	29.91140380848088	28.702359826426083	18.40712972059578
100	22.127574682951085	30.73141999051282	22.44185531482	24.699150011716092
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1673.0
1	1647.0
2	3140.0
3	4915.0
4	6659.5
5	11575.0
6	15791.0
7	18409.5
8	19844.5
9	18859.0
10	16620.0
11	14205.5
12	15045.5
13	20301.5
14	33519.0
15	53275.0
16	70141.5
17	79713.5
18	81114.0
19	75592.0
20	66586.0
21	56022.0
22	47583.0
23	45064.5
24	48360.5
25	57656.5
26	72199.5
27	89125.0
28	103617.0
29	121355.0
30	140108.0
31	157441.0
32	180356.5
33	203963.0
34	227803.5
35	251339.0
36	279573.5
37	305679.0
38	316034.5
39	322832.0
40	337674.5
41	347710.0
42	348852.0
43	357130.0
44	368892.5
45	378140.5
46	387485.5
47	393169.0
48	404600.5
49	398141.0
50	370335.5
51	346889.0
52	316669.0
53	280432.5
54	242033.5
55	202431.5
56	168314.0
57	139905.5
58	114330.0
59	92799.0
60	72312.0
61	54380.0
62	40741.0
63	29915.0
64	22383.5
65	16657.5
66	12446.5
67	9478.0
68	7152.0
69	5243.5
70	3886.0
71	2806.5
72	2048.5
73	1577.5
74	1203.0
75	843.5
76	550.5
77	369.5
78	242.0
79	155.0
80	91.5
81	60.0
82	48.5
83	38.0
84	31.5
85	26.5
86	21.0
87	17.5
88	12.0
89	8.0
90	6.0
91	4.0
92	3.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.32227404505769E-5
2	4.180093281103842E-4
3	2.32227404505769E-5
4	2.32227404505769E-5
5	2.32227404505769E-5
6	1.161137022528845E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.006763623156230522
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.9037154665475136E-5
26-27	0.0
28-29	1.162507573010271E-5
30-31	1.1638888809679784E-5
32-33	1.1662023820616614E-5
34-35	0.00168915660819691
36-37	0.037148213104707783
38-39	0.11889314044511734
40-41	0.15530339211606375
42-43	0.05522708615912023
44-45	0.0016361555503830215
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.008805560988728882
64-65	0.07177931985650315
66-67	0.17638822970429713
68-69	0.19550569581545363
70-71	0.07538793766254605
72-73	0.005166677348969072
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	132.0
20-21	569.0
22-23	1399.0
24-25	2467.0
26-27	3994.0
28-29	7827.0
30-31	16453.0
32-33	19108.0
34-35	24697.0
36-37	27193.0
38-39	29403.0
40-41	41263.0
42-43	50092.0
44-45	59473.0
46-47	62158.0
48-49	50098.0
50-51	42769.0
52-53	38545.0
54-55	58405.0
56-57	63748.0
58-59	64254.0
60-61	76368.0
62-63	82740.0
64-65	94895.0
66-67	106188.0
68-69	89021.0
70-71	77842.0
72-73	73278.0
74-75	74422.0
76-77	69690.0
78-79	73427.0
80-81	77775.0
82-83	79430.0
84-85	81953.0
86-87	88312.0
88-89	95604.0
90-91	95953.0
92-93	97437.0
94-95	106333.0
96-97	280740.0
98-99	527657.0
100-101	5599136.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.97984908044944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.79549826640034	66.0452450832946
2	8.72119039989378	13.427118415713753
3	2.662787263062558	6.149428849316962
4	1.131545483678034	3.484248022447969
5	0.589216396766541	2.267889464940727
6	0.33507608942665273	1.5476464076718544
7	0.2082975552639338	1.122430005763094
8	0.13660601761947788	0.8412728495862897
9	0.09214357599122001	0.6383878716183337
>10	0.3189373689223435	3.693731854012605
>50	0.006220069386423394	0.320170323253235
>100	0.0022851122090641433	0.31602905363166717
>500	9.820071808236964E-5	0.05362187784664571
>1k	9.820066147031044E-5	0.0927799209022371
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.161137022528845E-5	0.0
10-11	0.0	0.0	0.0	5.2251166013798023E-5	0.0
12-13	0.0	0.0	0.0	6.96682213517307E-5	0.0
14-15	0.0	0.0	0.0	8.708527668966338E-5	0.0
16-17	0.0	0.0	0.0	1.3353075759081715E-4	0.0
18-19	0.0	0.0	0.0	2.0319897894254788E-4	0.0
20-21	0.0	0.0	0.0	3.1931268119543235E-4	5.805685112644225E-6
22-23	0.0	0.0	0.0	8.476300264460568E-4	1.161137022528845E-5
24-25	0.0	0.0	0.0	0.0019855443085243247	1.161137022528845E-5
26-27	0.0	0.0	0.0	0.00366919299119115	1.161137022528845E-5
28-29	0.0	0.0	0.0	0.00813376484281456	1.161137022528845E-5
30-31	0.0	0.0	0.0	0.008923338018134174	1.161137022528845E-5
32-33	0.0	0.0	0.0	0.010444427517646961	1.161137022528845E-5
34-35	0.0	0.0	0.0	0.012006156812948258	1.161137022528845E-5
36-37	0.0	0.0	0.0	0.01448518435604734	1.161137022528845E-5
38-39	0.0	0.0	0.0	0.02047665139229618	1.161137022528845E-5
40-41	0.0	0.0	0.0	0.026619066241473772	1.161137022528845E-5
42-43	0.0	0.0	0.0	0.030874633429041988	1.161137022528845E-5
44-45	0.0	0.0	0.0	0.035942996532380395	1.161137022528845E-5
46-47	0.0	0.0	0.0	0.041319060946688954	1.161137022528845E-5
48-49	0.0	0.0	0.0	0.04779239984728726	1.161137022528845E-5
50-51	0.0	0.0	0.0	0.05448054909705341	1.161137022528845E-5
52-53	0.0	0.0	0.0	0.0740399022415518	1.161137022528845E-5
54-55	0.0	0.0	0.0	0.09854569910202307	1.161137022528845E-5
56-57	0.0	0.0	0.0	0.12796310556779136	1.161137022528845E-5
58-59	0.0	0.0	0.0	0.14469509006243203	1.161137022528845E-5
60-61	0.0	0.0	0.0	0.15691025153943547	1.161137022528845E-5
62-63	0.0	0.0	0.0	0.1698453179704068	1.161137022528845E-5
64-65	0.0	0.0	0.0	0.17465823092878885	1.161137022528845E-5
66-67	0.0	0.0	0.0	0.17999946123242155	1.161137022528845E-5
68-69	0.0	0.0	0.0	0.18616509882204973	1.161137022528845E-5
70-71	0.0	0.0	0.0	0.19102445726133294	1.161137022528845E-5
72-73	0.0	0.0	0.0	0.19490265491657927	1.161137022528845E-5
74-75	0.0	0.0	0.0	0.1980551419327451	1.161137022528845E-5
76-77	0.0	0.0	0.0	0.20117279483823503	1.161137022528845E-5
78-79	0.0	0.0	0.0	0.20336734381081456	1.161137022528845E-5
80-81	0.0	0.0	0.0	0.20411627719034564	1.161137022528845E-5
82-83	0.0	0.0	0.0	0.20477812529318712	1.161137022528845E-5
84-85	0.0	0.0	0.0	0.205155494825509	1.161137022528845E-5
86-87	0.0	0.0	0.0	0.2054109449704653	1.161137022528845E-5
88	0.0	0.0	0.0	0.20542836202580325	1.161137022528845E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14570	0.0	52.015324	1
CTTATAC	10250	0.0	40.85381	1
GTATCAA	27700	0.0	38.3963	1
TATACAC	13175	0.0	34.15601	3
ATCAACG	34525	0.0	30.222954	3
TCAACGC	34870	0.0	29.997602	4
TATCAAC	35445	0.0	29.716501	2
CAACGCA	35450	0.0	29.50681	5
TTATACA	15070	0.0	29.264528	2
AACGCAG	35985	0.0	29.143406	6
ACGCAGA	40235	0.0	26.054514	7
CGCAGAG	40460	0.0	25.877869	8
GCAGAGT	46275	0.0	22.598248	9
TACACAT	20755	0.0	21.764303	5
ATACACA	22780	0.0	20.731792	4
ACATGGG	25535	0.0	20.607773	3
GAGTACT	33060	0.0	20.43858	12-13
GTACATG	27715	0.0	20.300093	1
ACACATC	20985	0.0	20.181822	6
TACATGG	26995	0.0	20.064333	2
>>END_MODULE