FastQCFastQC Report
Fri 27 May 2016
SRR2139129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8564386
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA279300.031.8630541
GTATCAA433950.028.1159671
CAACGCA495250.023.967575
AACGCAG497250.023.9405276
ATCAACG498650.023.7792593
TCAACGC503450.023.6100654
TATCAAC521100.023.0803242
CGCAGAG538650.022.039428
ACGCAGA546750.021.6977737
GAGTACT359100.019.92764912-13
GCAGAGT595750.019.8228539
GTACATG319400.019.4948331
TACATGG320900.018.9137782
AGAGTAC508200.018.678210-11
ACATGGG315750.018.3967743
GTACTTT392600.018.25902714-15
CAGAGTA568600.018.00036410-11
AGTACTT382550.017.62991712-13
GTGCGCT9700.017.0633589
CATGGGG195850.016.6675344
Produced by FastQC (version 0.11.5)