##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139129_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8564386
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64587852532569	33.0	33.0	33.0	33.0	33.0
2	32.654644127436576	33.0	33.0	33.0	33.0	33.0
3	32.62171742375927	33.0	33.0	33.0	33.0	33.0
4	36.56155397479749	37.0	37.0	37.0	37.0	37.0
5	36.58907176766671	37.0	37.0	37.0	37.0	37.0
6	36.56645777058624	37.0	37.0	37.0	37.0	37.0
7	36.47061447253779	37.0	37.0	37.0	37.0	37.0
8	36.50752920291075	37.0	37.0	37.0	37.0	37.0
9	36.56035820898311	37.0	37.0	37.0	37.0	37.0
10-11	36.5415941084393	37.0	37.0	37.0	37.0	37.0
12-13	36.52915626409178	37.0	37.0	37.0	37.0	37.0
14-15	38.58348438522037	40.0	37.0	40.0	37.0	40.0
16-17	38.524741411701896	40.0	37.0	40.0	37.0	40.0
18-19	38.56280485256036	40.0	37.0	40.0	37.0	40.0
20-21	38.57765009076553	40.0	37.0	40.0	37.0	40.0
22-23	38.56066495871022	40.0	37.0	40.0	37.0	40.0
24-25	38.57856698878156	40.0	37.0	40.0	37.0	40.0
26-27	38.497416808734485	40.0	37.0	40.0	37.0	40.0
28-29	38.38187420850555	40.0	37.0	40.0	37.0	40.0
30-31	38.227986123585524	40.0	37.0	40.0	37.0	40.0
32-33	38.13071802996464	40.0	37.0	40.0	37.0	40.0
34-35	38.10295957629268	40.0	37.0	40.0	37.0	40.0
36-37	38.08121395335388	40.0	37.0	40.0	37.0	40.0
38-39	38.05333680277839	40.0	37.0	40.0	37.0	40.0
40-41	38.03191462759149	40.0	37.0	40.0	35.0	40.0
42-43	37.98829431947739	40.0	37.0	40.0	33.0	40.0
44-45	37.890812814560356	40.0	37.0	40.0	33.0	40.0
46-47	37.805204276276584	40.0	37.0	40.0	33.0	40.0
48-49	37.752805100767915	40.0	37.0	40.0	33.0	40.0
50-51	37.723913660025644	40.0	37.0	40.0	33.0	40.0
52-53	37.66838997567865	40.0	37.0	40.0	33.0	40.0
54-55	37.581542682718776	40.0	37.0	40.0	33.0	40.0
56-57	37.42214537393525	40.0	37.0	40.0	33.0	40.0
58-59	37.22299782873619	38.5	37.0	40.0	33.0	40.0
60-61	37.06731695676875	37.0	37.0	40.0	33.0	40.0
62-63	36.9391178093169	37.0	37.0	40.0	33.0	40.0
64-65	36.7774539258303	37.0	37.0	40.0	33.0	40.0
66-67	36.571482276141765	37.0	37.0	40.0	33.0	40.0
68-69	36.3703241952066	37.0	37.0	40.0	33.0	40.0
70-71	36.15445186151553	37.0	37.0	38.5	33.0	40.0
72-73	35.86284122151617	37.0	37.0	37.0	33.0	40.0
74-75	35.51983603711564	37.0	33.0	37.0	33.0	40.0
76-77	33.36566982212292	35.0	33.0	37.0	27.0	37.0
78-79	35.021058447786835	37.0	33.0	37.0	33.0	37.0
80-81	35.23550512624863	37.0	33.0	37.0	33.0	37.0
82-83	35.10301609757917	37.0	33.0	37.0	33.0	37.0
84-85	34.93415017439142	37.0	33.0	37.0	33.0	37.0
86-87	34.82282774108556	37.0	33.0	37.0	33.0	37.0
88-89	34.7299973737674	37.0	33.0	37.0	33.0	37.0
90-91	34.64650205103193	37.0	33.0	37.0	33.0	37.0
92-93	34.6360155935984	37.0	33.0	37.0	33.0	37.0
94-95	34.59954835906969	37.0	33.0	37.0	33.0	37.0
96-97	34.60806970210305	37.0	33.0	37.0	33.0	37.0
98-99	34.62788309259366	37.0	33.0	37.0	33.0	37.0
100	34.587471482061325	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	3.0
19	17.0
20	45.0
21	271.0
22	980.0
23	2900.0
24	6764.0
25	13522.0
26	24035.0
27	38643.0
28	58585.0
29	83383.0
30	114781.0
31	154776.0
32	208615.0
33	281309.0
34	392810.0
35	576277.0
36	1026070.0
37	2525328.0
38	2963532.0
39	91737.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3711083749738	20.601782848388996	15.041161741327603	22.985947035309596
2	14.12923187003626	20.99215142571107	43.074040109019265	21.804576595233407
3	17.955731789264494	27.027217949683486	31.133817547903327	23.883232713148697
4	12.054525806581557	18.18968904363828	39.824342319968096	29.931442829812067
5	12.592801467939612	38.09559005112451	35.93624060571775	13.375367875218128
6	28.318367284983104	38.57275215908673	19.225466860726133	13.883413695204034
7	25.2391502717358	33.54418326593211	23.97755355463352	17.239112907698566
8	23.608793859687534	36.94658752496531	21.50336539050965	17.94125322483751
9	25.296305572437483	17.264111783858386	22.288103582452212	35.15147906125192
10-11	23.799411890122656	27.03352581259182	29.071021553675884	20.09604074360964
12-13	24.857143557762974	24.70061334620606	29.628448306262538	20.813794789768426
14-15	21.9136141224835	25.87638506718403	28.07113668160216	24.138864128730305
16-17	20.487545750506808	29.092400786232663	28.69648215295294	21.723571310307594
18-19	20.34196613744406	27.940304185262082	31.150061428805287	20.567668248488566
20-21	21.339766505668038	27.00797348415404	31.08167046840162	20.570589541776307
22-23	21.104785557181327	26.757461023562456	31.06513288335006	21.07262053590616
24-25	20.736915477136268	27.07990222498499	31.2211169919866	20.96206530589214
26-27	20.714958017726538	27.224206109651227	31.256389616816428	20.804446255805807
28-29	20.547477495383962	27.500268194119432	31.097772052663615	20.85448225783299
30-31	20.847097631584486	27.294722837056355	30.947866095952648	20.91031343540651
32-33	20.61867763376961	27.46134925871332	30.878663193818678	21.04130991369839
34-35	20.77422384775768	27.59218505408007	30.774732811663043	20.858858286499206
36-37	20.840774200169673	27.484285388137074	30.773877075494045	20.90106333619921
38-39	20.786638791986455	27.680513329812463	30.6830167086372	20.849831169563878
40-41	20.932402564227655	27.718564401521522	30.496483741676812	20.852549292574007
42-43	20.95109105654976	27.838081042210266	29.954466630674048	21.25636127056593
44-45	21.027201382874395	28.102762988821013	29.562898007518108	21.307137620786484
46-47	21.25406939710678	28.06632844884614	29.148461826130756	21.53114032791632
48-49	21.333449736487704	28.17878636260911	28.92989759610144	21.557866304801745
50-51	21.099273965695946	28.33445212879335	28.88533323956925	21.68094066594145
52-53	21.323566466569705	28.364965512130897	28.554767653341568	21.756700367957833
54-55	21.31251262708873	28.287332439227058	28.62416809912566	21.77598683455855
56-57	21.399149189229014	28.325437826631227	28.42200203678062	21.853410947359137
58-59	21.41023956437263	28.317693426703656	28.547926547910542	21.724140461013178
60-61	21.550600202915685	28.34303792323057	28.53150103881209	21.574860835041658
62-63	21.393215501388983	28.36769086199779	28.579952913445645	21.65914072316758
64-65	21.352445067623908	28.390475047059716	28.469256704288743	21.787823181027637
66-67	21.413331945673104	28.44835831504889	28.182599089006633	21.955710650271378
68-69	21.422772588335448	28.347787069325353	28.25782096802228	21.97161937431692
70-71	21.562242772929714	28.055293199868792	28.299375383770858	22.083088643430635
72-73	21.591295201139346	28.024224084660908	28.437787406712495	21.946693307487244
74-75	21.44572864219168	28.219124285665607	28.34217341707751	21.992973655065203
76-77	21.509420040716805	28.092553959115158	28.190881347862096	22.207144652305942
78-79	21.660110683280205	28.008726449678623	28.14563763532398	22.185525231717193
80-81	21.616937098215097	28.04497540979714	28.227243160134876	22.110844331852896
82-83	21.83254686693807	27.7869243338551	28.38351952453789	21.99700927466894
84-85	22.022073782851937	27.858628550432744	28.1019444064825	22.017353260232817
86-87	21.626345734600747	28.00637017599989	28.23242133626009	22.134862753139277
88-89	21.83303064627782	27.812215770403142	28.180553830890265	22.174199752428777
90-91	21.980653336007688	27.56752302705972	28.300429208172197	22.151394428760398
92-93	22.005775940140644	27.718804671712903	28.1675820445295	22.10783734361695
94-95	21.811907510313223	27.54478930187861	28.342017300590143	22.30128588721803
96-97	21.951257624401567	27.714956400458462	28.35639108286248	21.977394892277484
98-99	23.04894488775023	29.64771460299669	28.57788726985906	18.72545323939402
100	22.09276539190573	30.444591940589316	22.127508255807076	25.33513441169788
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1819.0
1	1885.5
2	3710.0
3	5792.5
4	8318.5
5	15196.5
6	20774.0
7	23378.5
8	24073.5
9	22244.0
10	19495.0
11	16676.5
12	17765.5
13	24298.5
14	40209.5
15	63453.0
16	83580.0
17	95254.0
18	96982.5
19	90421.0
20	79373.5
21	66485.5
22	56295.0
23	52811.5
24	56099.5
25	66279.5
26	80580.0
27	95244.5
28	110257.5
29	128516.0
30	147659.5
31	165316.5
32	187885.5
33	211754.0
34	232552.0
35	253506.5
36	280010.5
37	305573.5
38	317506.5
39	323972.5
40	335454.0
41	346142.5
42	357705.0
43	373995.5
44	391744.5
45	409805.5
46	420427.5
47	423987.5
48	419986.0
49	403440.5
50	379428.5
51	351055.0
52	319840.5
53	288501.5
54	256629.5
55	221958.5
56	187571.5
57	155558.0
58	126962.0
59	103319.0
60	80337.5
61	60768.0
62	45843.0
63	33831.5
64	24923.5
65	18172.5
66	13527.5
67	10298.5
68	7679.5
69	5581.0
70	4071.5
71	2967.5
72	2159.0
73	1510.5
74	1054.5
75	734.0
76	512.0
77	347.5
78	221.0
79	144.0
80	101.0
81	71.5
82	56.0
83	36.5
84	27.0
85	20.5
86	14.0
87	12.0
88	9.0
89	5.5
90	3.5
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.1676260271314254E-5
2	3.269352875967991E-4
3	1.1676260271314254E-5
4	1.1676260271314254E-5
5	1.1676260271314254E-5
6	1.1676260271314254E-5
7	1.1676260271314254E-5
8	1.1676260271314254E-5
9	1.1676260271314254E-5
10-11	0.0
12-13	0.006351885587594953
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.1682438835277203E-5
26-27	0.0
28-29	5.849380670499298E-6
30-31	1.1720737260159286E-5
32-33	1.175987395767092E-5
34-35	0.0017417834906439363
36-37	0.0362517990831014
38-39	0.11632512624521575
40-41	0.1532325605609956
42-43	0.0540755942415712
44-45	0.0015559317644889816
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.009227933219456324
64-65	0.07473298641378522
66-67	0.18352059716752614
68-69	0.20395227791024845
70-71	0.07800722773043799
72-73	0.005405905393776299
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	394.0
20-21	1458.0
22-23	2026.0
24-25	3427.0
26-27	6739.0
28-29	12257.0
30-31	26700.0
32-33	33325.0
34-35	42786.0
36-37	59692.0
38-39	72609.0
40-41	81085.0
42-43	96218.0
44-45	112949.0
46-47	107763.0
48-49	91961.0
50-51	76965.0
52-53	67837.0
54-55	74740.0
56-57	77712.0
58-59	78409.0
60-61	76749.0
62-63	73286.0
64-65	74741.0
66-67	83208.0
68-69	82291.0
70-71	81650.0
72-73	78461.0
74-75	78755.0
76-77	74510.0
78-79	80035.0
80-81	83118.0
82-83	87051.0
84-85	90397.0
86-87	91309.0
88-89	98988.0
90-91	99182.0
92-93	98421.0
94-95	107808.0
96-97	265012.0
98-99	491738.0
100-101	5140624.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.28239873694625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.99164059707083	68.9690593659809
2	8.164871578376932	12.946612082256792
3	2.348732609406454	5.58639465796292
4	1.0092424920539724	3.2006066270916933
5	0.5350164628153777	2.1208694267879675
6	0.3006255043791706	1.430058666521099
7	0.1812713297427849	1.0060138090970754
8	0.12522334709045932	0.794240586816065
9	0.08516403815716479	0.6076808308102359
>10	0.2545512650265897	3.036829195524069
>50	0.002756252078376196	0.14424429655281243
>100	8.171618905279897E-4	0.11735666608252612
>500	8.736191131119813E-5	0.04003378851586452
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1676260271314254E-5
2	0.0	0.0	0.0	0.0	1.1676260271314254E-5
3	0.0	0.0	0.0	0.0	1.1676260271314254E-5
4	0.0	0.0	0.0	0.0	1.1676260271314254E-5
5	0.0	0.0	0.0	0.0	1.1676260271314254E-5
6	0.0	0.0	0.0	0.0	1.1676260271314254E-5
7	0.0	0.0	0.0	0.0	1.1676260271314254E-5
8	0.0	0.0	0.0	0.0	1.1676260271314254E-5
9	0.0	0.0	0.0	2.335252054262851E-5	1.1676260271314254E-5
10-11	0.0	0.0	0.0	7.589569176354265E-5	1.1676260271314254E-5
12-13	0.0	0.0	0.0	1.4011512325577105E-4	2.9190650678285636E-5
14-15	0.0	0.0	0.0	3.969928492246846E-4	4.086691094959989E-5
16-17	0.0	0.0	0.0	8.406907395346263E-4	4.670504108525702E-5
18-19	0.0	0.0	0.0	0.0016288383078483385	4.670504108525702E-5
20-21	0.0	0.0	0.0	0.0038706802799406753	5.2543171220914144E-5
22-23	0.0	0.0	0.0	0.007828932511916208	5.838130135657127E-5
24-25	0.0	0.0	0.0	0.013883073462592647	5.838130135657127E-5
26-27	0.0	0.0	0.0	0.01906149489292052	5.838130135657127E-5
28-29	0.0	0.0	0.0	0.031012147280610657	5.838130135657127E-5
30-31	0.0	0.0	0.0	0.03396040299911751	5.838130135657127E-5
32-33	0.0	0.0	0.0	0.0396817705320615	7.005756162788553E-5
34-35	0.0	0.0	0.0	0.04638394392779588	7.005756162788553E-5
36-37	0.0	0.0	0.0	0.05622119320637814	7.005756162788553E-5
38-39	0.0	0.0	0.0	0.08134266718011075	7.005756162788553E-5
40-41	0.0	0.0	0.0	0.10832066653698233	7.005756162788553E-5
42-43	0.0	0.0	0.0	0.12620869727263578	7.005756162788553E-5
44-45	0.0	0.0	0.0	0.1476638255211757	7.589569176354265E-5
46-47	0.0	0.0	0.0	0.16944588905731245	8.173382189919978E-5
48-49	0.0	0.0	0.0	0.18940645599112418	8.173382189919978E-5
50-51	0.0	0.0	0.0	0.20940205170574983	1.0508634244182829E-4
52-53	0.0	0.0	0.0	0.2531588370725	1.0508634244182829E-4
54-55	0.0	0.0	0.0	0.3008797128013614	1.0508634244182829E-4
56-57	0.0	0.0	0.0	0.3479467179550291	1.0508634244182829E-4
58-59	0.0	0.0	0.0	0.3848904054534674	1.0508634244182829E-4
60-61	0.0	0.0	0.0	0.40042566974445104	1.1676260271314254E-4
62-63	0.0	0.0	0.0	0.4200184344797163	1.284388629844568E-4
64-65	0.0	0.0	0.0	0.4270884100739971	1.4011512325577105E-4
66-67	0.0	0.0	0.0	0.4327338819151776	1.4011512325577105E-4
68-69	0.0	0.0	0.0	0.43857785018097034	1.4011512325577105E-4
70-71	0.0	0.0	0.0	0.44335927876207354	1.4595325339142816E-4
72-73	0.0	0.0	0.0	0.44766781880218853	1.6930577393405667E-4
74-75	0.0	0.0	0.0	0.45124075444521067	1.8682016434102807E-4
76-77	0.0	0.0	0.0	0.45434663967738026	1.8682016434102807E-4
78-79	0.0	0.0	0.0	0.4564250140056742	1.8682016434102807E-4
80-81	0.0	0.0	0.0	0.4570555320603252	1.8682016434102807E-4
82-83	0.0	0.0	0.0	0.45762766881361955	2.3352520542628508E-4
84-85	0.0	0.0	0.0	0.45801298540257296	2.3352520542628508E-4
86-87	0.0	0.0	0.0	0.4581706149162357	2.568777259689136E-4
88	0.0	0.0	0.0	0.45818812930664266	2.802302465115421E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	27930	0.0	31.863054	1
GTATCAA	43395	0.0	28.115967	1
CAACGCA	49525	0.0	23.96757	5
AACGCAG	49725	0.0	23.940527	6
ATCAACG	49865	0.0	23.779259	3
TCAACGC	50345	0.0	23.610065	4
TATCAAC	52110	0.0	23.080324	2
CGCAGAG	53865	0.0	22.03942	8
ACGCAGA	54675	0.0	21.697773	7
GAGTACT	35910	0.0	19.927649	12-13
GCAGAGT	59575	0.0	19.822853	9
GTACATG	31940	0.0	19.494833	1
TACATGG	32090	0.0	18.913778	2
AGAGTAC	50820	0.0	18.6782	10-11
ACATGGG	31575	0.0	18.396774	3
GTACTTT	39260	0.0	18.259027	14-15
CAGAGTA	56860	0.0	18.000364	10-11
AGTACTT	38255	0.0	17.629917	12-13
GTGCGCT	970	0.0	17.063358	9
CATGGGG	19585	0.0	16.667534	4
>>END_MODULE