FastQCFastQC Report
Fri 27 May 2016
SRR2139128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8471872
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA281850.033.446581
GTATCAA446300.029.2652931
ATCAACG525400.024.5035823
TATCAAC549700.023.6125132
ACGCAGA547000.023.5832187
AACGCAG548450.023.5131766
CAACGCA549900.023.4642985
TCAACGC553000.023.368834
ACGGTAC8250.022.173293
ACGTGCG8400.021.2957888
CGCAGAG609600.021.2281428
ACATGGG313150.020.017213
TACATGG325850.019.7983152
GAGTACT399400.019.72582812-13
GCAGAGT654400.019.6257679
GTACATG336700.019.5828881
TGCGAGT16600.019.45364494
GTACTAG22650.019.383161
GGGCACC30250.018.97846894
AGAGTAC555050.018.83162110-11
Produced by FastQC (version 0.11.5)