##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139128_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8471872
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73436201585671	33.0	33.0	33.0	33.0	33.0
2	32.781463648175986	33.0	33.0	33.0	33.0	33.0
3	33.44965540083703	33.0	33.0	33.0	33.0	37.0
4	36.57323446341021	37.0	37.0	37.0	37.0	37.0
5	36.59645931855439	37.0	37.0	37.0	37.0	37.0
6	36.5589860186745	37.0	37.0	37.0	37.0	37.0
7	36.49857162620021	37.0	37.0	37.0	37.0	37.0
8	36.51800334093811	37.0	37.0	37.0	37.0	37.0
9	36.57557137312745	37.0	37.0	37.0	37.0	37.0
10-11	36.573618262882164	37.0	37.0	37.0	37.0	37.0
12-13	36.80685071729129	37.0	37.0	37.0	37.0	38.5
14-15	38.59536912266852	40.0	37.0	40.0	37.0	40.0
16-17	38.53727824263634	40.0	37.0	40.0	37.0	40.0
18-19	38.58611744842226	40.0	37.0	40.0	37.0	40.0
20-21	38.60311244534853	40.0	37.0	40.0	37.0	40.0
22-23	38.59164361108673	40.0	37.0	40.0	37.0	40.0
24-25	38.59391266282961	40.0	37.0	40.0	37.0	40.0
26-27	38.50521612294172	40.0	37.0	40.0	37.0	40.0
28-29	38.39310521548569	40.0	37.0	40.0	37.0	40.0
30-31	38.23328516931043	40.0	37.0	40.0	37.0	40.0
32-33	38.13644367208194	40.0	37.0	40.0	37.0	40.0
34-35	38.11636406354772	40.0	37.0	40.0	37.0	40.0
36-37	38.09402521746662	40.0	37.0	40.0	37.0	40.0
38-39	38.06631964627664	40.0	37.0	40.0	37.0	40.0
40-41	38.04663312324354	40.0	37.0	40.0	37.0	40.0
42-43	37.999591066113766	40.0	37.0	40.0	33.0	40.0
44-45	37.897244567640996	40.0	37.0	40.0	33.0	40.0
46-47	37.81802127402725	40.0	37.0	40.0	33.0	40.0
48-49	37.76621948629419	40.0	37.0	40.0	33.0	40.0
50-51	37.735876624013414	40.0	37.0	40.0	33.0	40.0
52-53	37.6829242364453	40.0	37.0	40.0	33.0	40.0
54-55	37.591444046946556	40.0	37.0	40.0	33.0	40.0
56-57	37.42412026375236	40.0	37.0	40.0	33.0	40.0
58-59	37.223949273034	37.0	37.0	40.0	33.0	40.0
60-61	37.075790364744456	37.0	37.0	40.0	33.0	40.0
62-63	36.947781414391855	37.0	37.0	40.0	33.0	40.0
64-65	36.78454486495169	37.0	37.0	40.0	33.0	40.0
66-67	36.58784063980846	37.0	37.0	40.0	33.0	40.0
68-69	36.385156258296995	37.0	37.0	40.0	33.0	40.0
70-71	36.169675450555495	37.0	37.0	38.5	33.0	40.0
72-73	35.863169618103996	37.0	37.0	37.0	33.0	40.0
74-75	35.255783271877874	37.0	33.0	37.0	30.0	40.0
76-77	33.65601533363575	35.0	33.0	37.0	27.0	37.0
78-79	35.11392384097964	37.0	33.0	37.0	33.0	37.0
80-81	35.282521158235326	37.0	33.0	37.0	33.0	37.0
82-83	35.14118683192656	37.0	33.0	37.0	33.0	37.0
84-85	34.97920393223321	37.0	33.0	37.0	33.0	37.0
86-87	34.87295178691906	37.0	33.0	37.0	33.0	37.0
88-89	34.78316069348064	37.0	33.0	37.0	33.0	37.0
90-91	34.700573720946686	37.0	33.0	37.0	33.0	37.0
92-93	34.68266819229422	37.0	33.0	37.0	33.0	37.0
94-95	34.65740756527218	37.0	33.0	37.0	33.0	37.0
96-97	34.65707638994026	37.0	33.0	37.0	33.0	37.0
98-99	34.6650033417414	37.0	33.0	37.0	33.0	37.0
100	34.600662902069615	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	1.0
18	8.0
19	14.0
20	69.0
21	234.0
22	918.0
23	2654.0
24	6046.0
25	12337.0
26	21830.0
27	35754.0
28	54076.0
29	77312.0
30	107099.0
31	146485.0
32	198237.0
33	270177.0
34	378909.0
35	561666.0
36	1010502.0
37	2491348.0
38	2967298.0
39	128897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.33054244745724	0.022533417092960202	25.939704872435364	28.707219263014434
2	14.912801207859111	23.883558358983972	39.71344390108936	21.490196532067557
3	16.2129376395058	25.973262101519502	33.62961188025784	24.184188378716858
4	12.445268872161636	23.374213721409795	38.62364507481819	25.556872331610375
5	16.490951820554375	37.06832139775516	32.65221255755813	13.788514224132333
6	26.08409641742656	38.39962860624294	20.81331266729628	14.702962309034215
7	24.755924635774083	34.025730561761385	23.278399777333718	17.939945025130815
8	23.117724158249793	32.21756655435776	23.26173011112538	21.402979176267063
9	23.595598324052418	21.036137973886266	24.282696228362493	31.085567473698823
10-11	24.2469077599005	26.162847177998554	29.09461315703647	20.495631905064478
12-13	24.81623411450078	24.738992269854332	29.17242744138854	21.272346174256352
14-15	21.929462579226882	26.9205436531619	28.17148323298558	22.97851053462564
16-17	19.9860845395209	29.147170778784194	29.33689271981446	21.529851961880446
18-19	19.939725246084926	27.926124237948823	31.807155490545657	20.32699502542059
20-21	21.075479290787392	26.981381994500808	31.727379213622083	20.21575950108972
22-23	20.849070036129383	26.811185776248113	31.60383673702621	20.735907450596297
24-25	20.482067074365702	27.065689434471558	31.97008784733033	20.482155643832414
26-27	20.382536295085316	27.399342916000336	31.704252767079133	20.513868021835215
28-29	20.18976763576387	27.586690298451842	31.594641537665463	20.62890052811882
30-31	20.612342601900234	27.319232115748836	31.590749081112584	20.477676201238346
32-33	20.298383526264953	27.771380747380665	31.345339832295632	20.58489589405875
34-35	20.388676569499516	27.740598374822095	31.297597283534657	20.573127772143728
36-37	20.63991465924791	27.602595311271305	31.245133429875484	20.5123565996053
38-39	20.40673405082417	27.98750349199171	31.105283907520924	20.500478549663196
40-41	20.78453927910605	27.78177451840505	30.781691140246735	20.651995062242165
42-43	20.779978442510203	27.85988936639064	30.375634132920037	20.98449805817912
44-45	20.721320673605344	28.493897012775886	29.820058007019167	20.9647243065996
46-47	21.166158828463306	28.12945500428859	29.301071102941073	21.403315064307034
48-49	21.13284063752272	28.415112689864287	29.19766168856667	21.25438498404633
50-51	20.960836042768996	28.618850534691454	28.814342087303274	21.605971335236276
52-53	21.166069617412223	28.460880568533454	28.691533369755934	21.68151644429839
54-55	21.175165379623774	28.51950829944351	28.59478437958091	21.710541941351806
56-57	21.38733969141039	28.669578909885256	28.30354527407501	21.639536124629345
58-59	21.4022513085942	28.671258490934225	28.48863177609849	21.437858424373083
60-61	21.379171336826698	28.447079222061305	28.580780822916996	21.592968618195
62-63	21.334090267176016	28.589408129770703	28.45515466105169	21.621346942001587
64-65	21.252230831934405	28.59535628087776	28.331230906556552	21.82118198063128
66-67	21.432759798099976	28.58032679547126	28.16942651430428	21.81748689212448
68-69	21.358700353119808	28.53651651574428	28.393189439835798	21.711593691300116
70-71	21.59457386376655	27.94194970229688	28.403933149060283	22.05954328487629
72-73	21.507691902527288	28.256057179650206	28.46083241544848	21.77541850237403
74-75	21.365583249348912	28.41761535663725	28.505752594968936	21.7110487990449
76-77	21.533230232981847	28.20714275636193	28.153151564264622	22.1064754463916
78-79	21.440162566359223	28.142903147975968	28.3799221414117	22.037012144253104
80-81	21.47227919518178	28.249629114033013	28.370175905466137	21.90791578531907
82-83	21.915647551964046	27.756975486871678	28.50445472174353	21.822922239420745
84-85	22.02530790263147	27.8808832395137	28.37238459263565	21.721424265219177
86-87	21.57390099926315	28.218049098299993	28.231448644372932	21.976601258063933
88-89	21.866200290950907	27.75040623507225	28.229045336317927	22.15434813765892
90-91	22.027763052738937	27.630777797536915	28.311531551643714	22.02992759808043
92-93	21.845309569865353	27.907934751196695	28.322088149588414	21.924667529349538
94-95	21.884416011343856	27.574789357855888	28.352657234613492	22.188137396186765
96-97	21.79085121082402	28.001611585359633	28.517929157950263	21.689608045866088
98-99	23.09209165622923	29.95430675837093	27.885792686371836	19.067808899028
100	22.341571768060962	29.940049176204024	22.28188260249943	25.436496453235584
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1968.0
1	2103.0
2	4535.5
3	7338.5
4	10922.5
5	20040.5
6	26962.0
7	29711.0
8	29972.0
9	27020.0
10	23006.0
11	19113.5
12	20261.5
13	27490.5
14	45991.5
15	75242.0
16	101654.0
17	116428.0
18	117091.0
19	107312.0
20	92607.5
21	76537.0
22	64371.5
23	59524.5
24	61364.0
25	70425.0
26	85503.5
27	102229.0
28	116754.5
29	133955.0
30	151957.0
31	168255.5
32	190642.0
33	215000.0
34	240149.5
35	265816.0
36	294053.0
37	322454.0
38	333622.5
39	335736.5
40	344602.0
41	352115.5
42	356437.5
43	366290.5
44	379030.5
45	393483.5
46	402610.0
47	408084.5
48	410812.0
49	393223.5
50	363346.0
51	335327.5
52	307207.5
53	281261.5
54	252945.5
55	215889.5
56	179899.0
57	147901.0
58	121056.0
59	100546.5
60	79895.0
61	59698.0
62	43247.0
63	31378.0
64	22634.0
65	15739.5
66	11415.5
67	8739.0
68	6640.5
69	4825.5
70	3537.5
71	2550.0
72	1792.5
73	1338.5
74	1051.5
75	736.5
76	508.0
77	348.5
78	223.0
79	156.0
80	110.0
81	68.0
82	45.5
83	30.5
84	19.5
85	13.5
86	11.0
87	9.0
88	7.0
89	5.0
90	2.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.1803766629146427E-4
2	2.7148663247036784E-4
3	2.3607533258292856E-5
4	2.3607533258292856E-5
5	2.3607533258292856E-5
6	1.1803766629146428E-5
7	1.1803766629146428E-5
8	0.0
9	9.443013303317142E-5
10-11	0.0011921804295437891
12-13	0.005046110233960097
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.903107921386423E-6
24-25	2.9523146855690384E-5
26-27	0.0
28-29	2.3660171791775345E-5
30-31	4.150147104928525E-5
32-33	2.2021051649245832E-4
34-35	0.004892460664287304
36-37	0.04555118377674476
38-39	0.12248555610624288
40-41	0.13956788113851532
42-43	0.04560475904481447
44-45	0.001083854686341906
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	1.3612003718254934E-5
58-59	5.506606792413245E-5
60-61	7.864738195741362E-4
62-63	0.016199288117991054
64-65	0.0889475976074142
66-67	0.18500481666362548
68-69	0.18472896076560466
70-71	0.06756003680903022
72-73	0.004466399545549457
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	230.0
20-21	1122.0
22-23	1883.0
24-25	3817.0
26-27	8735.0
28-29	15606.0
30-31	30332.0
32-33	38663.0
34-35	52396.0
36-37	71003.0
38-39	83622.0
40-41	89472.0
42-43	109323.0
44-45	126602.0
46-47	120994.0
48-49	102275.0
50-51	86206.0
52-53	77588.0
54-55	83792.0
56-57	84074.0
58-59	80192.0
60-61	79464.0
62-63	76206.0
64-65	81771.0
66-67	88038.0
68-69	86315.0
70-71	88272.0
72-73	83969.0
74-75	80456.0
76-77	79587.0
78-79	84182.0
80-81	84028.0
82-83	83763.0
84-85	91299.0
86-87	97859.0
88-89	102767.0
90-91	100439.0
92-93	99389.0
94-95	124996.0
96-97	370814.0
98-99	1360334.0
100-101	3859997.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.9473407632525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.43395175351426	66.50862739092189
2	8.078607270527971	12.4325469307561
3	2.5410795340678503	5.865879384433373
4	1.1597963651392307	3.5697298449740003
5	0.6159966148989857	2.3699650717821137
6	0.3459004456942004	1.5969671678995534
7	0.2169688402041793	1.1686622697539064
8	0.1568562074537012	0.9655734436617099
9	0.10407126802320277	0.7207206591820651
>10	0.34016161784826693	3.867664568355822
>50	0.004352680281967426	0.21947893226428047
>100	0.0017284759426418861	0.24611320380951207
>500	3.1735586086479374E-4	0.17544189542146826
>1k	2.1157054266206108E-4	0.2926292367842675
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	1.1803766629146428E-5	0.0
20-21	1.1803766629146428E-5	0.0	0.0	3.541129988743928E-5	0.0
22-23	2.3607533258292856E-5	0.0	0.0	5.9018833145732136E-5	0.0
24-25	2.3607533258292856E-5	0.0	0.0	1.0033201634774462E-4	0.0
26-27	2.3607533258292856E-5	0.0	0.0	2.3017344926835534E-4	0.0
28-29	2.3607533258292856E-5	0.0	0.0	7.259316476925053E-4	0.0
30-31	2.3607533258292856E-5	0.0	0.0	0.0020066403269548925	0.0
32-33	2.3607533258292856E-5	0.0	0.0	0.004225748453234421	0.0
34-35	2.3607533258292856E-5	0.0	0.0	0.0066691281454677315	0.0
36-37	2.3607533258292856E-5	0.0	0.0	0.01067060503274837	0.0
38-39	2.3607533258292856E-5	0.0	0.0	0.016814465563219087	0.0
40-41	2.3607533258292856E-5	0.0	0.0	0.023094069409924985	0.0
42-43	3.541129988743928E-5	0.0	0.0	0.029686473072303265	0.0
44-45	3.541129988743928E-5	0.0	0.0	0.036869065066138865	0.0
46-47	3.541129988743928E-5	0.0	0.0	0.0441106758931202	0.0
48-49	3.541129988743928E-5	0.0	0.0	0.05142901120319098	0.0
50-51	3.541129988743928E-5	0.0	0.0	0.05790927908259237	0.0
52-53	3.541129988743928E-5	0.0	0.0	0.06480267879401388	0.0
54-55	3.541129988743928E-5	0.0	0.0	0.071341965506561	0.0
56-57	3.541129988743928E-5	0.0	0.0	0.07713761492147189	0.0
58-59	3.541129988743928E-5	0.0	0.0	0.08316933966896572	0.0
60-61	3.541129988743928E-5	0.0	0.0	0.08885285330089973	0.0
62-63	3.541129988743928E-5	0.0	0.0	0.09490818558165184	0.0
64-65	3.541129988743928E-5	0.0	0.0	0.10089269526262909	0.0
66-67	4.1313183202012496E-5	0.0	0.0	0.10748509892500736	0.0
68-69	4.721506651658571E-5	0.0	0.0	0.11437849863642888	0.0
70-71	4.721506651658571E-5	0.0	0.0	0.12015054051808148	0.0
72-73	4.721506651658571E-5	0.0	0.0	0.12417562493862042	0.0
74-75	4.721506651658571E-5	0.0	0.0	0.12781118506039751	0.0
76-77	4.721506651658571E-5	0.0	0.0	0.13128739433268113	0.0
78-79	4.721506651658571E-5	0.0	0.0	0.13342387609255663	0.0
80-81	4.721506651658571E-5	0.0	0.0	0.13407898514047428	0.0
82-83	4.721506651658571E-5	0.0	0.0	0.13459244898884215	0.0
84-85	4.721506651658571E-5	0.0	0.0	0.13485803373799793	0.0
86-87	4.721506651658571E-5	0.0	0.0	0.1349996789375477	0.0
88	5.9018833145732136E-5	0.0	0.0	0.13501148270417684	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	28185	0.0	33.44658	1
GTATCAA	44630	0.0	29.265293	1
ATCAACG	52540	0.0	24.503582	3
TATCAAC	54970	0.0	23.612513	2
ACGCAGA	54700	0.0	23.583218	7
AACGCAG	54845	0.0	23.513176	6
CAACGCA	54990	0.0	23.464298	5
TCAACGC	55300	0.0	23.36883	4
ACGGTAC	825	0.0	22.17329	3
ACGTGCG	840	0.0	21.295788	8
CGCAGAG	60960	0.0	21.228142	8
ACATGGG	31315	0.0	20.01721	3
TACATGG	32585	0.0	19.798315	2
GAGTACT	39940	0.0	19.725828	12-13
GCAGAGT	65440	0.0	19.625767	9
GTACATG	33670	0.0	19.582888	1
TGCGAGT	1660	0.0	19.453644	94
GTACTAG	2265	0.0	19.38316	1
GGGCACC	3025	0.0	18.978468	94
AGAGTAC	55505	0.0	18.831621	10-11
>>END_MODULE