FastQCFastQC Report
Fri 27 May 2016
SRR2139127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17534262
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA424800.044.8164941
GTATCAA758050.034.1131481
ATCAACG897150.028.2788933
ACGCAGA934950.027.1701937
AACGCAG939450.027.0482586
TCAACGC940650.027.0276154
TATCAAC947100.027.006972
CAACGCA943000.026.922295
CGCAGAG1061050.023.9718918
TGCGAGT32600.022.71428394
GCAGAGT1177200.021.4785759
ACATGGG715400.020.7846763
GTACATG753950.020.6408061
GGTTCAC73900.020.5703146
GTGGTAT205000.020.553521
TACATGG752100.020.296062
GAGTACT773550.019.77135312-13
TGATCGC26350.019.3336289
ACGGTAC20300.019.069793
CAGAGTA1159100.019.02212710-11
Produced by FastQC (version 0.11.5)